LOCUS       sequence-311075-        4948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of SRDX-EL222 in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Ochoa-Fernandez R, Abel NB, Wieland FG, Schlegel J, Koch LA, Miller 
            JB, Engesser R, Giuriani G, Brandl SM, Timmer J, Weber W, Ott T, 
            Simon R, Zurbriggen MD
  TITLE     Optogenetic control of gene expression in plants in the presence of 
            ambient white light.
  JOURNAL   Nat Methods. 2020 Jul;17(7):717-725. doi: 10.1038/s41592-020-0868-y.
            Epub 2020 Jun 29.
  PUBMED    32601426
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0868-y"; journalName: "Nat Methods"; date: 
            "2020-07"; volume: "17"; issue: "7"; pages: "717-725"
COMMENT     Sequence Label: pROF051
FEATURES             Location/Qualifiers
     source          1..4948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             439..459
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      1250..1502
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(1553..1571)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1592..1608)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1592..1608)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1605..1627)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1616..1632
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1640..1646,1647..1664,1665..1670))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1685..1706
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1823..1840)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1994..2582)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2074..2093)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2753..3544,3545..3613))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3376..3395
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3614..3718)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3744..4199)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3881..3902)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4093..4112
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4326..4348
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4340..4357
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4341..4357
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4364..4383
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4364..4382
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatcacca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctagc
      361 gctaccggtt ggctaggtaa gcttggtacc acctgaacga cgcatatgct tgatcttgaa
      421 cttagacttg gttttgctcc aaagaagaag aggaaggtgg gagctggagc tggggcagac
      481 gacacacgcg ttgaggtgca accgccggcg cagtgggtcc tcgacctgat cgaggccagc
      541 ccgatcgcat cggtcgtgtc cgatccgcgt ctcgccgaca atccgctgat cgccatcaac
      601 caggccttca ccgacctgac cggctattcc gaagaagaat gcgtcggccg caattgccga
      661 ttcctggcag gttccggcac cgagccgtgg ctgaccgaca agatccgcca aggcgtgcgc
      721 gagcacaagc cggtgctggt cgagatcctg aactacaaga aggacggcac gccgttccgc
      781 aatgccgtgc tcgttgcacc gatctacgat gacgacgacg agcttctcta tttcctcggc
      841 agccaggtcg aagtcgacga cgaccagccc aacatgggca tggcgcgccg cgaacgcgcc
      901 gcggaaatgc tcaagacgct gtcgccgcgc cagctcgagg ttacgacgct ggtggcatcg
      961 ggcttgcgca acaaggaagt ggcggcccgg ctcggcctgt cggagaaaac cgtcaagatg
     1021 caccgcgggc tggtgatgga aaagctcaac ctgaagacca gtgccgatct ggtgcgcatt
     1081 gccgtcgaag ccggaatcta aactagataa gtaatgatca taatcagcca tatcacatct
     1141 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc cgaattcccc gggctcgaga
     1201 agcttggatc caccggatct agataactga tctcgaggcg aatttccccg atcgttcaaa
     1261 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     1321 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     1381 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     1441 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     1501 tcgggaatta attcgataag ctagagatcc gtcaaggtac cagcttttgt tccctttagt
     1561 gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     1621 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     1681 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1741 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1801 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1861 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1921 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1981 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2041 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2101 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2161 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2221 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2281 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2341 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2401 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2461 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2521 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2581 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2641 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2701 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2761 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2821 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2881 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2941 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     3001 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     3061 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     3121 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     3181 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     3241 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     3301 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     3361 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     3421 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3481 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     3541 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     3601 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3661 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3721 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     3781 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     3841 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     3901 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     3961 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     4021 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     4081 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     4141 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     4201 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     4261 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     4321 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
     4381 gggcgaattg gagctccacc gcggagcgga gaattaaggg agtcaggcct taattaagag
     4441 ctcgcatgcc tgcaggtccc cagattagcc ttttcaattt cagaaagaat gctaacccac
     4501 agatggttag agaggcttac gcagcaggtc tcatcaagac gatctacccg agcaataatc
     4561 tccaggaaat caaatacctt cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta
     4621 actgcatcaa gaacacagag aaagatatat ttctcaagat cagaagtact attccagtat
     4681 ggacgattca aggcttgctt cacaaaccaa ggcaagtaat agagattgga gtctctaaaa
     4741 aggtagttcc cactgaatca aaggccatgg agtcaaagat tcaaatagag gacctaacag
     4801 aactcgccgt aaagactggc gaacagttca tacagagtct cttacgactc aatgacaaga
     4861 agaaaatctt cgtcaacatg gtggagcacg acacacttgt ctactccaaa aatatcaaag
     4921 atacagtctc agaagaccaa agggcaat
//
