LOCUS       sequence-311083-       11504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for expression of the PULSE system under the control 
            of AtUbi10 promoter. It contains a plant selection cassette. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11504)
  AUTHORS   Ochoa-Fernandez R, Abel NB, Wieland FG, Schlegel J, Koch LA, Miller 
            JB, Engesser R, Giuriani G, Brandl SM, Timmer J, Weber W, Ott T, 
            Simon R, Zurbriggen MD
  TITLE     Optogenetic control of gene expression in plants in the presence of 
            ambient white light.
  JOURNAL   Nat Methods. 2020 Jul;17(7):717-725. doi: 10.1038/s41592-020-0868-y.
            Epub 2020 Jun 29.
  PUBMED    32601426
REFERENCE   2  (bases 1 to 11504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0868-y"; journalName: "Nat Methods"; date: 
            "2020-07"; volume: "17"; issue: "7"; pages: "717-725"
COMMENT     Sequence Label: pROF148
FEATURES             Location/Qualifiers
     source          1..11504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..23
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      complement(69..321)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(361..1155)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(472..491)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1082..1101
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1189..1368)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2129..2149
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      2815..3067
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             4876..4896
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4923..5175
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             8193..8426
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             8427..8447
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8474..8726
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8963..8987
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(9078..9666)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9158..9177)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9837..10652)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9837..10652)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10560..10579
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10644..10663
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10943..11378
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tggcaggata tattgtggtg taacaagctt cgtctcagtc aggagagcga tcagcttgca
       61 tgccggtcga tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg
      121 ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac
      181 ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa
      241 cagaaattat atgataatca tggcaagacc ggcaacagga ttcaatctta agaaacttta
      301 ttgccaaatg tttgaacgat ctgcttgact ctagctagag tccgaacccc agagtcccgc
      361 tcagaagaac tcgtcaagaa ggcgatagaa ggctatgcgc tgcgaatcgg gagcggcgat
      421 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
      481 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
      541 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcgtcgc cgtgggtcac
      601 gacgagatcc tcgccgtcgg gcatccgcgc cttgagcctg gcgaacagtt cggctggcgc
      661 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
      721 acgtgctcgc tcgatgcggt gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
      781 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
      841 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
      901 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
      961 cgctgcctcg tcttggagtt cattcagggc accggacagg tcggtcttga caaaaagaac
     1021 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
     1081 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
     1141 atcttgttca atcatgcctc gatcgagttg agagtgaata tgagactcta attggatacc
     1201 gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata
     1261 gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat
     1321 taaactccag aaacccgcgg ctcagtggct ccttcaacgt tgcggttctg tcagttccaa
     1381 acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata ccgtgactcc cttaattctc
     1441 atgtatctcc gtcaggaggt cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc
     1501 ggcacacacg agtcgtgttt atcaactcaa agcacaaata cttttcctca acctaaaaat
     1561 aaggcaatta gccaaaaaca actttgcgtg taaacaacgc tcaatacacg tgtcatttta
     1621 ttattagcta ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct cgtcttttct
     1681 tcttcttctt ctataaaaca atacccaaag agctcttctt cttcacaatt cagatttcaa
     1741 tttctcaaaa tcttaaaaac tttctctcaa ttctctctac cgtgatcaag gtaaatttct
     1801 gtgttcctta ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat
     1861 gttctttggt ttagattctg ttaatcttag atcgaagacg attttctggg tttgatcgtt
     1921 agatatcatc ttaattctcg attagggttt catagatatc atccgatttg ttcaaataat
     1981 ttgagttttg tcgaataatt actcttcgat ttgtgatttc tatctagatc tggtgttagt
     2041 ttctagtttg tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta acagaatgct
     2101 tgatcttgaa cttagacttg gttttgctcc aaagaagaag aggaaggtgg gagctggagc
     2161 tggggcagac gacacacgcg ttgaggtgca accgccggcg cagtgggtcc tcgacctgat
     2221 cgaggccagc ccgatcgcat cggtcgtgtc cgatccgcgt cttgccgaca atccgctgat
     2281 cgccatcaac caggccttca ccgacctgac cggctattcc gaagaagaat gcgtcggccg
     2341 caattgccga ttcctggcag gttccggcac cgagccgtgg ctgaccgaca agatccgcca
     2401 aggcgtgcgc gagcacaagc cggtgctggt cgagatcctg aactacaaga aggacggcac
     2461 gccgttccgc aatgccgtgc tcgttgcacc gatctacgat gacgacgacg agcttctcta
     2521 tttcctcggc agccaggtcg aagtcgacga cgaccagccc aacatgggca tggcgcgccg
     2581 cgaacgcgcc gcggaaatgc tcaagacgct gtcgccgcgc cagctcgagg ttacgacgct
     2641 ggtggcatcg ggcttgcgca acaaggaagt ggcggcccgg ctcggcctgt cggagaaaac
     2701 cgtcaagatg caccgcgggc tggtgatgga aaagctcaac ctgaagacca gtgccgatct
     2761 ggtgcgcatt gccgtcgaag ccggaatcta agcttggaat ggatcttcga tcccgatcgt
     2821 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgacgatt
     2881 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     2941 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     3001 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     3061 ctagatcggg aattgccaag ctaattcttg aagacgaaag ggcctcgtga tacgcctatt
     3121 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     3181 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3241 catgagacaa taaccctgat aaatgcttca ataatgggac cgactcgcgc tgtcaggagg
     3301 tcgacgagtc agtaataaac ggcgtcaaag tggttgcagc cggcacacac gagtcgtgtt
     3361 tatcaactca aagcacaaat acttttcctc aacctaaaaa taaggcaatt agccaaaaac
     3421 aactttgcgt gtaaacaacg ctcaatacac gtgtcatttt attattagct attgcttcac
     3481 cgccttagct ttctcgtgac ctagtcgtcc tcgtcttttc ttcttcttct tctataaaac
     3541 aatacccaaa gagctcttct tcttcacaat tcagatttca atttctcaaa atcttaaaaa
     3601 ctttctctca attctctcta ccgtgatcaa ggtaaatttc tgtgttcctt attctctcaa
     3661 aatcttcgat tttgttttcg ttcgatccca atttcgtata tgttctttgg tttagattct
     3721 gttaatctta gatcgaagac gattttctgg gtttgatcgt tagatatcat cttaattctc
     3781 gattagggtt tcatagatat catccgattt gttcaaataa tttgagtttt gtcgaataat
     3841 tactcttcga tttgtgattt ctatctagat ctggtgttag tttctagttt gtgcgatcga
     3901 atttgtcgat taatctgagt ttttctgatt aacagaatga caacaatgcc ccgccccaag
     3961 ctcaagtccg atgacgaggt actcgaggcc gccaccgtag tgctgaagcg ttgcggtccc
     4021 atagagttca cgctcagcgg agtagcaaag gaggtggggc tctcccgcgc agcgttaatc
     4081 cagcgcttca ccaaccgcga tacgctgctg gtgaggatga tggagcgcgg cgtcgagcag
     4141 gtgcggcatt acctgaatgc gataccgata ggcgcagggc cgcaagggct ctgggaattt
     4201 ttgcaggtgc tcgttcggag catgaacact cgcaacgact tctcggtgaa ctatctcatc
     4261 tcctggtacg agctccaggt gccggagcta cgcacgcttg cgatccagcg gaaccgcgcg
     4321 gtggtggagg ggatccgcaa gcgactgccc ccaggtgctc ctgcggcagc tgagttgctc
     4381 ctgcactcgg tcatcgctgg cgcgacgatg cagtgggccg tcgatccgga tggtgagcta
     4441 gctgatcatg tgctggctca gatcgctgcc atcctgtgtt taatgtttcc cgaacacgac
     4501 gatttccaac tcctccaggc acatgcgtcc gcgtacagct taattaacag tgctggtagt
     4561 gctggtagtg ctggtatgat gttcttacca accgattatt gttgcaggtt aagcgatcaa
     4621 gagtatatgg agcttgtgtt tgagaatggc cagattcttg caaagggcca aagatccaac
     4681 gtttctctgc ataatcaacg taccaaatcg atcatggatt tgtatgaggc agagtataac
     4741 gaggatttca tgaagagtat catccatggt ggtggtggtg ccatcacaaa tctcggggac
     4801 acgcaggttg ttccacaaag tcatgttgct gctgcccatg aaacaaacat gttggaaagc
     4861 aataaacatg ttgacccaaa gaagaagagg aaggtgtagg cttggaatgg atcttcgatc
     4921 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     4981 cgacgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     5041 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     5101 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     5161 ctatgttact agatcgggaa ttgccaagct aattcttgaa gacgaaaggg cctcgtgata
     5221 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     5281 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5341 tatccgctca tgagacaata accctgataa atgcttcaat aatgggaccg actcgcgctg
     5401 tcaggaggtc gacgagtcag taataaacgg cgtcaaagtg gttgcagccg gcacacacga
     5461 gtcgtgttta tcaactcaaa gcacaaatac ttttcctcaa cctaaaaata aggcaattag
     5521 ccaaaaacaa ctttgcgtgt aaacaacgct caatacacgt gtcattttat tattagctat
     5581 tgcttcaccg ccttagcttt ctcgtgacct agtcgtcctc gtcttttctt cttcttcttc
     5641 tataaaacaa tacccaaaga gctcttcttc ttcacaattc agatttcaat ttctcaaaat
     5701 cttaaaaact ttctctcaat tctctctacc gtgatcaagg taaatttctg tgttccttat
     5761 tctctcaaaa tcttcgattt tgttttcgtt cgatcccaat ttcgtatatg ttctttggtt
     5821 tagattctgt taatcttaga tcgaagacga ttttctgggt ttgatcgtta gatatcatct
     5881 taattctcga ttagggtttc atagatatca tccgatttgt tcaaataatt tgagttttgt
     5941 cgaataatta ctcttcgatt tgtgatttct atctagatct ggtgttagtt tctagtttgt
     6001 gcgatcgaat ttgtcgatta atctgagttt ttctgattaa cagaatgaca acaatggttt
     6061 ccggagtcgg gggtagtggc ggtggccgtg gcggtggccg tggcggagaa gaagaaccgt
     6121 cgtcaagtca cactcctaat aaccgaagag gaggagaaca agctcaatcg tcgggaacga
     6181 aatctctcag accaagaagc aacactgaat caatgagcaa agcaattcaa cagtacaccg
     6241 tcgacgcaag actccacgcc gttttcgaac aatccggcga atcagggaaa tcattcgact
     6301 actcacaatc actcaaaacg acgacgtacg gttcctctgt acctgagcaa cagatcacag
     6361 cttatctctc tcgaatccag cgaggtggtt acattcagcc tttcggatgt atgatcgccg
     6421 tcgatgaatc cagtttccgg atcatcggtt acagtgaaaa cgccagagaa atgttaggga
     6481 ttatgcctca atctgttcct actcttgaga aacctgagat tctagctatg ggaactgatg
     6541 tgagatcttt gttcacttct tcgagctcga ttctactcga gcgtgctttc gttgctcgag
     6601 agattacctt gttaaatccg gtttggatcc attccaagaa tactggtaaa ccgttttacg
     6661 ccattcttca taggattgat gttggtgttg ttattgattt agagccagct agaactgaag
     6721 atcctgcgct ttctattgct ggtgctgttc aatcgcagaa actcgcggtt cgtgcgattt
     6781 ctcagttaca ggctcttcct ggtggagata ttaagctttt gtgtgacact gtcgtggaaa
     6841 gtgtgaggga cttgactggt tatgatcgtg ttatggttta taagtttcat gaagatgagc
     6901 atggagaagt tgtagctgag agtaaacgag acgatttaga gccttatatt ggactgcatt
     6961 atcctgctac tgatattcct caagcgtcaa ggttcttgtt taagcagaac cgtgtccgaa
     7021 tgatagtaga ttgcaatgcc acacctgttc ttgtggtcca ggacgatagg ctaactcagt
     7081 ctatgtgctt ggttggttct actcttaggg ctcctcatgg ttgtcactct cagtatatgg
     7141 ctaacatggg atctattgcg tctttagcaa tggcggttat aatcaatgga aatgaagatg
     7201 atgggagcaa tgtagctagt ggaagaagct cgatgaggct ttggggtttg gttgtttgcc
     7261 atcacacttc ttctcgctgc ataccgtttc cgctaaggta tgcttgtgag tttttgatgc
     7321 aggctttcgg tttacagtta aacatggaat tgcagttagc tttgcaaatg tcagagaaac
     7381 gcgttttgag aacgcagaca ctgttatgtg atatgcttct gcgtgactcg cctgctggaa
     7441 ttgttacaca gagtcccagt atcatggact tagtgaaatg tgacggtgca gcatttcttt
     7501 accacgggaa gtattacccg ttgggtgttg ctcctagtga agttcagata aaagatgttg
     7561 tggagtggtt gcttgcgaat catgcggatt caaccggatt aagcactgat agtttaggcg
     7621 atgcggggta tcccggtgca gctgcgttag gggatgctgt gtgcggtatg gcagttgcat
     7681 atatcacaaa aagagacttt cttttttggt ttcgatctca cactgcgaaa gaaatcaaat
     7741 ggggaggcgc taagcatcat ccggaggata aagatgatgg gcaacgaatg catcctcgtt
     7801 cgtcctttca ggcttttctt gaagttgtta agagccggag tcagccatgg gaaactgcgg
     7861 aaatggatgc gattcactcg ctccagctta ttctgagaga ctcttttaaa gaatctgagg
     7921 cggctatgaa ctctaaagtt gtggatggtg tggttcagcc atgtagggat atggcggggg
     7981 aacaggggat tgatgagtta ggtgaattcg atagtgctgg tagtgctggt agtgctggtt
     8041 ccgcgtacag ccgcgcgcgt acgaaaaaca attacgggtc taccatcgag ggcctgctcg
     8101 atctcccgga cgacgacgcc cccgaagagg cggggctggc ggctccgcgc ctgtcctttc
     8161 tccccgcggg acacacgcgc agactgtcga cggccccccc gaccgatgtc agcctggggg
     8221 acgagctcca cttagacggc gaggacgtgg cgatggcgca tgccgacgcg ctagacgatt
     8281 tcgatctgga catgttgggg gacggggatt ccccgggtcc gggatttacc ccccacgact
     8341 ccgcccccta cggcgctctg gatatggccg acttcgagtt tgagcagatg tttaccgatg
     8401 cccttggaat tgacgagtac ggtgggccaa agaagaagag gaaggtgtag gcttggaatg
     8461 gatcttcgat cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     8521 tgccggtctt gcgacgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     8581 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     8641 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     8701 cgcggtgtca tctatgttac tagatcggga attgccaagc taattcttga agacgaaagg
     8761 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     8821 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     8881 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatgggacc
     8941 gactcgcgct tgagacgaag cttgacagga tatattggcg ggtaaactaa gtcgctgtat
     9001 gtgtttgttt gagatctcat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9061 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9121 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9181 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9241 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9301 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9361 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9421 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9481 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9541 ctgctgaagc cagttacctt cggaagaaga gttggtagct cttgatccgg caaacaaacc
     9601 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9661 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9721 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9781 taaaaatgaa gttttaaatc aatctaaagt atatatgtgt aacattggtc tagtgattag
     9841 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     9901 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     9961 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
    10021 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
    10081 tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca
    10141 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
    10201 tgagcgagtc gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
    10261 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
    10321 tctaatacct ggaatgctgt tttccctggg atcgcagtgg tgagtaacca tgcatcatca
    10381 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
    10441 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
    10501 tctggcgcat cgggcttccc atacaatcgg tagattgtcg cacctgattg cccgacatta
    10561 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctt
    10621 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
    10681 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga
    10741 ttttgagaca caacgtggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag
    10801 atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc
    10861 aactggtcca cctacaacaa agctctcatc aaccgtggct ccctcacttt ctggctggat
    10921 gatggggcga ttcaggcgat ccccatccaa cagcccgccg tcgagcgggc ttttttatcc
    10981 ccggaagcct gtggatagag ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc
    11041 ttgattcacg gggctttccg gcccgctcca aaaactatcc acgtgaaatc gctaatcagg
    11101 gtacgtgaaa tcgctaatcg gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta
    11161 atcaaaaagg cacgtgagaa cgctaatagc cctttcagat caacagcttg caaacacccc
    11221 tcgctccggc aagtagttac agcaagtagt atgttcaatt agcttttcaa ttatgaatat
    11281 atatatcaat tattggtcgc ccttggcttg tggacaatgc gctacgcgca ccggctccgc
    11341 ccgtggacaa ccgcaagcgg ttgcccaccg tcgagcgcct ttgcccacaa cccggcggcc
    11401 ggccgcaaca gatcgtttta taaatttttt tttttgaaaa agaaaaagcc cgaaaggcgg
    11461 caacctctcg ggcttctgga tttccgatcc ccggaattag atct
//
