LOCUS       sequence-311093-       10950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi flaB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10950)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_P0026-sgRNAflaB
FEATURES             Location/Qualifiers
     source          1..10950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        1322..1397
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(2840..2854,2855..2860,2861..2877,2878..2884)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2860..2876
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2892..2908
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2927..2938
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2945..2971,2972..2974,2975..5461,5462..5464,
                     5465..7051)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7221..8012
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8135..8723
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8624..8643
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatctttatc aatatttaaa ttgaataaaa ttgtaagaaa aattgaaaga atttttttac
       61 ataaaaagtt acatttattg aagtaatttc ttacttgata cttttctatt tcattaatct
      121 ttctttcttc ttttatatta tttttattat ttaaacactc ctttaaatct acactcccat
      181 ttttagtaaa tctatcttta aggccccttg aaactctatt ttgaaatctt aaatcttttt
      241 tttcttgaaa gtatttatag attttttggt aacattcttt ttttgaaaaa ttaagttgat
      301 agtatatttc ggtccccaaa tttaccccta agtgtttgtg ataattagtt gtgactttaa
      361 attctttttc taatttgtaa aggtaatttt gcagtgtttt tagtttaacg ggtgtttgac
      421 tatttctttt aaggttttca ttaaagtaat agagtatgtt tttttgtgtg tatttcttat
      481 atgttttatt tatgtacgct agtgttgaga taaggactac taatttatac tgtaatttat
      541 tgtagcaagt tgtggtttta ctggttttgt ttatattaga taagttttcc atttatagct
      601 ccttgaatac cggatttttt atctataaag tatatatatc acaattttga tttaaaagta
      661 aattcttttt ttaaaaatac aaaaattttt tataaattat atagaccatg atattgtatt
      721 aatactaatt gattttaatt taaggttgaa ctaaaataaa catagttttg tagaaaataa
      781 tttttcatta ttttgctact tgatattgga tcgtaaaaat attagggctt tacaaagttc
      841 ttttaaaaga gaatttagta aagccctaag tcttttaata aaaaattttt tgtaaaaaag
      901 ttggcaaaaa tagtttttgc tatatattta tatataagaa aaaattcaat actcaaggag
      961 ataaaaaatg gaaaacttaa acaataatcc acaaaaaatt aatcaaataa ataagcaaac
     1021 taattttatt ggttttgaaa attttataga acttgataat caagttaatc aaactaaaga
     1081 aaaaagtaaa ttaagtaaga taggtaagaa attacccgga ataagcagtc aagtctagtg
     1141 cggccggaaa cagctatgac atgattacga attcgagctc tattctccat tcttttaaaa
     1201 ttattatcct tgtttattat agtttaaaat aaatttaatt acaatttgaa atttggtcgt
     1261 ttgactttcc ttatattgac aatctaagta taatattaag gaaaatttaa atttctgact
     1321 tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
     1381 tggcaccgag tcggtgcttt ttttggcgcg ccaaataatg cccgtgtccc tgttgaatag
     1441 ggacacggtt ttttaacaat ttttttattc ctaggcctta gtggccggta cccggggatc
     1501 cttattactg gccgctttct agcctggata cttgtctggc tagttgcatt agagagtcag
     1561 ctagagccct agggctagct gtttgtgtat tcggagccag tgtagttttt ctttttacta
     1621 acgatacggg taataattga ttgcctttta cagcctgacc ttgagacaat tgcaaaagtc
     1681 tgtccacaga ggtttgacct aataatctaa aatcttgttt gatagtagta agtggaggta
     1741 tgtagcaact agaatcttct gtatcatcat aacccacaac tgaaatatca gccccaactc
     1801 taagtccact ttcagtaata gctcgcatag ctcctaaagc catttgatca ttagcaacta
     1861 acatagcagt agggacaatg ccttcattta acatttgcat tgtttgctga aatcctgaca
     1921 tagcagacca atctccttct ctttctgcta ttggttgtat ttgatttctt gttaaatact
     1981 tgtgccatcc tgctaatctc aatctggcac ttactgaact taagggacct gcaagtagtg
     2041 caatttgttg atggcctaat gcaacaaggt gttcaactcc taaccttgtt ccatcttcgt
     2101 gagaaaaaat tatagagttt attggggttt ggtccgacac atccaaaaac aatgctggaa
     2161 cattagtaca tgctgcttca actgcaatcg catcttggtc atcaagagga taattaatta
     2221 taagtccact aacacgttga gctaataaat tatgtactgc tgccttacaa gcctctacac
     2281 ctgatctctc aaccattgat acaacaacgc ttgcaccaag ttgatcagct ctcgatttaa
     2341 ttgcggctac aatttgacta ggcgcatgta atgcaagtga tgatgtggca actccaatta
     2401 aaagtgattg tttccccgca agttgctgag caacgcgatt tggtatataa ttcaactctg
     2461 ccatagctgc ttcaaccttc tctcttgttt ttgcagatac atgacttgct tggttcacaa
     2521 ctctcgaaac cgtttggtag ctaaccccgg catattcagc aacatcatac aaagtaacag
     2581 gtttcatgga tcattcctcc atgataaaat ttaaatttct gactttggca aatccttttg
     2641 cctgttaaaa agaatagcac aaaaattaaa aaaaaagaaa cgcaaatagc aatagcatta
     2701 aaaataatta ttattattta tttctttgat taaaatcaaa agcctttaat gtattttctt
     2761 gatctttcat gcgcttaacg gtaaaatcca aggtattaag ttcaccttcc acttttctct
     2821 ctctatcttc tctagaaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat
     2881 aatagattca attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt
     2941 acatatggat aagaaatact caataggctt agctatcggc acaaatagcg tcggatgggc
     3001 ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga
     3061 ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc
     3121 ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat
     3181 ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca
     3241 tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt
     3301 tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg
     3361 aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc
     3421 gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag
     3481 tgatgtggac aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa
     3541 ccctattaac gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc
     3601 aagacgatta gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcttatttgg
     3661 gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc
     3721 agaagatgct aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt
     3781 ggcgcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc
     3841 tattttactt tcagatatcc taagagtaaa tactgaaata actaaggctc ccctatcagc
     3901 ttcaatgatt aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt
     3961 tcgacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata
     4021 tgcaggttat attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat
     4081 tttagaaaaa atggatggta ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct
     4141 gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact tgggtgagct
     4201 gcatgctatt ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa
     4261 gattgaaaaa atcttgactt ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa
     4321 tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga
     4381 agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga
     4441 taaaaatctt ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac
     4501 ggtttataac gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt
     4561 tctttcaggt gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt
     4621 aaccgttaag caattaaaag aagattattt caaaaaaata gaatgttttg atagtgttga
     4681 aatttcagga gttgaagata gatttaatgc ttcattaggt acctaccatg atttgctaaa
     4741 aattattaaa gataaagatt ttttggataa tgaagaaaat gaagatatct tagaggatat
     4801 tgttttaaca ttgaccttat ttgaagatag ggagatgatt gaggaaagac ttaaaacata
     4861 tgctcacctc tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt atactggttg
     4921 gggacgtttg tctcgaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat
     4981 attagatttt ttgaaatcag atggttttgc caatcgcaat tttatgcagc tgatccatga
     5041 tgatagtttg acatttaaag aagacattca aaaagcacaa gtgtctggac aaggcgatag
     5101 tttacatgaa catattgcaa atttagctgg tagccctgct attaaaaaag gtattttaca
     5161 gactgtaaaa gttgttgatg aattggtcaa agtaatgggg cggcataagc cagaaaatat
     5221 cgttattgaa atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgaga
     5281 gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga agtcagattc ttaaagagca
     5341 tcctgttgaa aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg
     5401 aagagacatg tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga
     5461 tgccattgtt ccacaaagtt tccttaaaga cgattcaata gacaataagg tcttaacgcg
     5521 ttctgataaa aatcgtggta aatcggataa cgttccaagt gaagaagtag tcaaaaagat
     5581 gaaaaactat tggagacaac ttctaaacgc caagttaatc actcaacgta agtttgataa
     5641 tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg
     5701 ccaattggtt gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat
     5761 gaatactaaa tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa
     5821 atctaaatta gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa
     5881 caattaccat catgcccatg atgcgtatct aaatgccgtc gttggaactg ctttgattaa
     5941 gaaatatcca aaacttgaat cggagtttgt ctatggtgat tataaagttt atgatgttcg
     6001 taaaatgatt gctaagtctg agcaagaaat aggcaaagca accgcaaaat atttctttta
     6061 ctctaatatc atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa
     6121 acgccctcta atcgaaacta atggggaaac tggagaaatt gtctgggata aagggcgaga
     6181 ttttgccaca gtgcgcaaag tattgtccat gccccaagtc aatattgtca agaaaacaga
     6241 agtccagaca ggcggattct ccaaggagtc aattttacca aaaagaaatt cggacaagct
     6301 tattgctcgt aaaaaagact gggatccaaa aaaatatggt ggttttgata gtccaacggt
     6361 agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc
     6421 cgttaaagag ttactaggga tcacaattct ggaaagaagt tcctttgaaa aaaatccgat
     6481 tgacttttta gaagctaaag gatataagga agttaaaaaa gacttaatca ttaaactacc
     6541 taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga
     6601 attacaaaaa ggaaatgagc tggctctgcc aagcaaatat gtgaattttt tatatttagc
     6661 tagtcattat gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt
     6721 ggagcagcat aagcattatt tagatgagat tattgagcaa atcagtgaat tttctaagcg
     6781 tgttatttta gcagatgcca atttagataa agttcttagt gcatataaca aacatagaga
     6841 caaaccaata cgtgaacaag cagaaaatat tattcattta tttacgttga cgaatcttgg
     6901 agctcccgct gcttttaaat attttgatac aacaattgat cgtaaacgat atacgtctac
     6961 aaaagaagtt ttagatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg
     7021 cattgatttg agtcagctag gaggtgacta ataaaagctt gatcagatct cagctttttt
     7081 ttgaagtgcc tggcagtaag ttgaaaaaat ttgaaataaa cttggactat gttaataaaa
     7141 aaatacaaat taacgaacaa aaagatttga attattatat aatttagtta aaagcaattt
     7201 taaatgaggg aggtttccat atgagggaag cggtgatcgc cgaagtatcg actcaactat
     7261 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     7321 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     7381 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     7441 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     7501 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     7561 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     7621 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     7681 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     7741 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     7801 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     7861 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     7921 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     7981 aaggcgagat caccaaggta gtcggcaaat aatgacgtcg ccggagcggt cgagttctgg
     8041 accgaccggc tcgggttctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     8101 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     8161 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8221 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     8281 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8341 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8401 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8461 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8521 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8581 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8641 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8701 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8761 tttgctggcc ttttgctcac atgttctttc ctgcactaga tccccggcaa gttcatcctt
     8821 agggttttca tatctcttaa aaagtcaatt tctgcttgat taatttcttg tggattattg
     8881 tttttgcggt ttttcatttt ttatctcctt gagtattgaa ttttttctta tatataaata
     8941 tatagcaaaa actatttttg ccaacttttt tacaaaaaat tttttattaa aacacttagg
     9001 gctttgctaa attctctttt aaaagaactt agtaaagccc tatggattta agaactgatt
     9061 atacttacgt agtaaaaata ctacagatat taatagtata gaccatatca aaatcataat
     9121 caatttatat ttatctaaaa ttgtagtcca tacaaaatac aaaaaaatta ggtttttcta
     9181 aattctattt taaaagaact tagtaacacc taatctctaa attacttaca aaattctaaa
     9241 attagatttt tagttcttca tatttcttta aaagtttttt aagaaaatct ttttgatttt
     9301 caaaaatttc atgcatcata aaactagtaa atttggattt ttttttgtaa aaatcataac
     9361 tttcttgggt tttaagttga aatcttaatg gttttattgg attttgccta gattttttta
     9421 acgccggact ttctttatct tttataaaat tataagaatt tttaatacca ttttctatta
     9481 aataatcttc ttttataagc ccctcttgca gtgcagttgc taatcttaaa tacttatacg
     9541 cttgagtttt tgctacatca aagtctttta taaaatcaag aaaagtttta taaccatcaa
     9601 atttataata ttgattatct tttatttctt ttaaaatttt aatattttcc accctgtgga
     9661 atatttcttt tttgaaattg tcatttaact tttcctttag tgtcttgtaa taagaaattt
     9721 tactttcatt aatcattaaa ttttgttcaa cataagtatc tatctttttt gttaaaactt
     9781 taacaacacg ttttttttcc ataaaaattt tcctttattt ttgcaacatt ttccttaatt
     9841 gatataattg ttactatttt agatttttta atatccactt aagaattaat ccctcactag
     9901 aatatttttt attatagaat tcatatacat gtttttctaa atctataaat ttatcaacaa
     9961 ttgctttatt atagggtgta tctatctttt cttttcttaa taatcgtctc atattcttta
    10021 aataacaaca aacactgccg gttttaaacc taaattctat gtaatatatt tttgaaaaag
    10081 tatatacatt ttcaaccccg tttatttcat actttacaaa aacattattt attggttttc
    10141 tataaccata gtaaatgcct aaaaatttgt ccccgtcttt cataggatat agataaagtt
    10201 tttgaatctc tgatttatct aataattttt caaaaaaaat tatacaccgc ttttgtttgt
    10261 tactaactac tctatgcaat gcctttaata tttttgtgtg atatatattt ttatcacctt
    10321 ttttttcttt cagcaggaag atactttctt ttggaaattt aattttttgt tcatttattc
    10381 ttgcagcctt aagtccgatt ttcatattta atccttatat agcctttcta tttaattctt
    10441 ttaaactcaa taaacttttt tggtttttca ttagttctaa taattcgcaa taatatgtgc
    10501 aaaacacttt attgtattct attttctttt ggttattcaa atattctttt ataattggct
    10561 ttagaagttc aatatttact tcttttttta gttgttcaat aaggatattg aaaatgtttg
    10621 tttttaaatc ttgataattt tgttgagaat tttctttttt tctttcaatt gacttctcta
    10681 atttacgctt tatattgttt aaatcgctat atttatgact ttcaataata aaatgaggct
    10741 taaatttgta attttcgtat attttttgga aacttgtttc taattgttta gaattatatt
    10801 catttttttc aaattctttt tgagtgttgc atagaatttc ctttaatttt tcttgttttt
    10861 ctttcataca agatttagta aaatgtatat ttttattttt tagtaattta atttctatta
    10921 tttttatgat tttaagtatt ttgattatat
//
