LOCUS       sequence-311099-        7270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector encoding PPARD cDNA for retroviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Legrand N, Bretscher CL, Zielke S, Wilke B, Daude M, Fritz B, 
            Diederich WE, Adhikary T
  TITLE     PPARbeta/delta recruits NCOR and regulates transcription 
            reinitiation of ANGPTL4.
  JOURNAL   Nucleic Acids Res. 2019 Oct 10;47(18):9573-9591. doi: 
            10.1093/nar/gkz685.
  PUBMED    31428774
REFERENCE   2  (bases 1 to 7270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-10-10- 10"; volume: "47"; issue: 
            "18"; pages: "9573-9591"
COMMENT     Sequence Label: pMSCVbsd-PPARD-T423D-T427A
FEATURES             Location/Qualifiers
     source          1..7270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        2891..3390
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2926..2949)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3323..3342
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3411..3809
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    4341..4357
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4365..4371,4372..4389,4390..4395))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4410..4431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4548..4565)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4719..5307)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4799..4818)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5478..6269,6270..6338))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6101..6120
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6339..6443)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6511..6529
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6567..6589)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6689..6708
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6902..6924
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7052..7071
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatccatg gagcagccac aggaggaagc
     1441 ccctgaggtc cgggaagagg aggagaaaga ggaagtggca gaggcagaag gagccccaga
     1501 gctcaatggg ggaccacagc atgcacttcc ttccagcagc tacacagacc tctcccggag
     1561 ctcctcgcca ccctcactgc tggaccaact gcagatgggc tgtgacgggg cctcatgcgg
     1621 cagcctcaac atggagtgcc gggtgtgcgg ggacaaggca tcgggcttcc actacggtgt
     1681 tcatgcatgt gaggggtgca agggcttctt ccgtcgtacg atccgcatga agctggagta
     1741 cgagaagtgt gagcgcagct gcaagattca gaagaagaac cgcaacaagt gccagtactg
     1801 ccgcttccag aagtgcctgg cactgggcat gtcacacaac gctatccgtt ttggtcggat
     1861 gccggaggct gagaagagga agctggtggc agggctgact gcaaacgagg ggagccagta
     1921 caacccacag gtggccgacc tgaaggcctt ctccaagcac atctacaatg cctacctgaa
     1981 aaacttcaac atgaccaaaa agaaggcccg cagcatcctc accggcaaag ccagccacac
     2041 ggcgcccttt gtgatccacg acatcgagac attgtggcag gcagagaagg ggctggtgtg
     2101 gaagcagttg gtgaatggcc tgcctcccta caaggagatc agcgtgcacg tcttctaccg
     2161 ctgccagtgc accacagtgg agaccgtgcg ggagctcact gagttcgcca agagcatccc
     2221 cagcttcagc agcctcttcc tcaacgacca ggttaccctt ctcaagtatg gcgtgcacga
     2281 ggccatcttc gccatgctgg cctctatcgt caacaaggac gggctgctgg tagccaacgg
     2341 cagtggcttt gtcacccgtg agttcctgcg cagcctccgc aaacccttca gtgatatcat
     2401 tgagcctaag tttgaatttg ctgtcaagtt caacgccctg gaacttgatg acagtgacct
     2461 ggccctattc attgcggcca tcattctgtg tggagaccgg ccaggcctca tgaacgttcc
     2521 acgggtggag gctatccagg acaccatcct gcgtgccctc gaattccacc tgcaggccaa
     2581 ccaccctgat gcccagtacc tcttccccaa gctgctgcag aagatggctg acctgcggca
     2641 actggtcacc gagcacgccc agatgatgca gcggatcaag aaggacgaaa ccgaggcctc
     2701 gctgcaccct ctgctccagg agatctacaa ggacatgtac taacggcggc acccaggcct
     2761 ccctgcagac tccaatgggg ccagcactgg aggggcccac ccacatgact tttccattga
     2821 ccagctctca agggcgaatt ctgcagatat ccatcacact ggcggccgct cgaggttaac
     2881 gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     2941 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     3001 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     3061 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     3121 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     3181 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     3241 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     3301 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     3361 ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc atggccaagc
     3421 ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc
     3481 ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca
     3541 ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca
     3601 ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca
     3661 ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga
     3721 tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc
     3781 tgccctctgg ttatgtgtgg gagggctaat cgataaaata aaagatttta tttagtctcc
     3841 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagag aaccatcaga
     3901 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
     3961 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
     4021 aacccctcac tcggcgcgcc agtcctccga tagactgcgt cgcccgggta cccgtgtatc
     4081 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
     4141 ctctgagtga ttgactaccc gtcagcgggg gtctttcatg ggtaacagtt tcttgaagtt
     4201 ggagaacaac attctgaggg taggagtcga atattaagta atcctgactc aattagccac
     4261 tgttttgaat ccacatactc caatactcct gaaatagttc attatggaca gcgcagaaag
     4321 agctggggag aattgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     4381 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     4441 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     4501 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     4561 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4621 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4681 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4741 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4801 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4861 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4921 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4981 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5041 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     5101 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     5161 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     5221 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     5281 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5341 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5401 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5461 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5521 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5581 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5641 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5701 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5761 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5821 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5881 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5941 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     6001 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     6061 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     6121 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     6181 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     6241 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     6301 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     6361 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6421 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     6481 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     6541 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     6601 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     6661 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     6721 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
     6781 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     6841 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     6901 tcccagtcac gacgttgtaa aacgacggcg caaggaatgg tgcatgcaag gagatggcgc
     6961 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     7021 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     7081 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg cgattagtcc
     7141 aatttgttaa agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc
     7201 agctgaagcc tatagagtac gagccataga taaaataaaa gattttattt agtctccaga
     7261 aaaagggggg
//
