LOCUS       sequence-311109-        7557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of FLuc under the control of AtAP1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Ochoa-Fernandez R, Abel NB, Wieland FG, Schlegel J, Koch LA, Miller 
            JB, Engesser R, Giuriani G, Brandl SM, Timmer J, Weber W, Ott T, 
            Simon R, Zurbriggen MD
  TITLE     Optogenetic control of gene expression in plants in the presence of 
            ambient white light.
  JOURNAL   Nat Methods. 2020 Jul;17(7):717-725. doi: 10.1038/s41592-020-0868-y.
            Epub 2020 Jun 29.
  PUBMED    32601426
REFERENCE   2  (bases 1 to 7557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0868-y"; journalName: "Nat Methods"; date: 
            "2020-07"; volume: "17"; issue: "7"; pages: "717-725"
COMMENT     Sequence Label: pROF366
FEATURES             Location/Qualifiers
     source          1..7557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3061..4713
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3127..3145)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    5457..5578
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5494..5513)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5548..5567
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5612..5629)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5783..6371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5863..5882)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6542..7333,7334..7402))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7165..7184
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7403..7507)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgtttaacat ccaagatttg ttttacataa tcgttacggt tactaaagtt taatttatga
       61 tgttttaaaa caaattgaga ctaaatttct aaaagaaaca tatacgtaca tgtgtgtagc
      121 tgcgtatata tatagaatgg tggggctaaa agctaatgat gtgtacatta attggacatt
      181 tgatgtggct ggattggacc caacttgctc tttgatagag acctaactaa gacaattttg
      241 ctcttcattc atttctcccg tatacataat tgaattaact gtacataatg tttcacaaca
      301 agcgatctag ctatatattt caaaataaca gagactgata ttttaatctg gtcttctaag
      361 ctctaacgtc aaattaaaaa aaaaatccga tcttctaatt aattagaaga aatcaattat
      421 agaacctctc tctttaattt catttattta aaactgcttg gaaatttaat tattcactaa
      481 agactcacta ttctccttaa tttatgataa tttgtagatc atatgttcag tttttattta
      541 tttgccattc gaatgttgag ttttaattaa accaatatgt taatattcga attaaaaaaa
      601 cttacctata attcacttat ttaaaaacat aaaataataa taattgcatc accgtgatac
      661 aaagcaacct cacaagtcac aactctcgtg actacaaaga tcactcatta aacaaacctt
      721 cctgccttct ttttttctac ttgggcacct cgaccgatcg aagactattc ttgggatctg
      781 cttcaaaaac gactatatgt tctaaatcca cttcgtatga tgacgaacat ttggtttact
      841 actgaagata gagattacgt ccttctaatt agaagtaatt aattatttta gtatttggaa
      901 gctaatggtg gagatgtaac cgtatcttag tggatcgaga tattgtatat aaaatatgta
      961 tgctacatcg aataataaac tgaaagagag taaaaaggga tatttaatgg gaagaaaaga
     1021 agggtggaga tgtaacaaag gcgaagataa tggatattct tgggatgttg tcttcaaggc
     1081 cacgagctta gattctttta gttttgctca atttgttaag tttctacttt tccttttgtt
     1141 gcttactact tttgctcatg atctccatat acatatcata catatatata gtatactatc
     1201 tttagactga tttctctata cactatcttt taacttatgt atcgtttcaa aactcaggac
     1261 gtacatgttt aaatttggtt atataaccac gaccatttca agtatatatg tcataccata
     1321 ccagatttaa tataacttct atgaagaaaa tacataaagt tggattaaaa tgcaagtgac
     1381 atctttttag cataggttca tttggcatag aagaaatata taactaaaaa tgaactttaa
     1441 cttaaataga ttttactata ttacaatttt ttctttttac atggtctaat ttatttttct
     1501 aaaattagta taattgttgt tttgatgaaa caataatacc gtaagcaata gttgctaaaa
     1561 gatgtccaaa tatttataaa ttacaaagta aatcaaataa ggaagaagac acgtggaaaa
     1621 caccaaataa gagaagaaat ggaaaaaaca gaaagaaatt ttttaacaag aaaaatcaat
     1681 tagtcctcaa acctgagata tttaaagtaa tcaactaaaa caggaacact tgactaacaa
     1741 agaaatttga aacgtggtcc aactttcact taattatatt gttttctcta aggcttatgc
     1801 aatatatgcc ttaagcaaat gccgaatctg tttttttttt tttgttattg gatattgact
     1861 gaaaataagg ggttttttca cacttgaaga tctcaaaaga gaaaactatt acaacggaaa
     1921 ttcattgtaa aagaagtgat taagcaaatt gagcaaaggt ttttatgtgg tttatttcat
     1981 tatatgattg acatcaaatt gtatatatat ggttgtttta tttaacaata tatatggata
     2041 taacgtacaa actaaatatg tttgattgac gaaaaaaaat atatgtatgt ttgattaaca
     2101 acatagcaca tattcaactg atttttgtcc tgatcatcta caacttaata agaacacaca
     2161 acattgaaca aatctttgac aaaatactat ttttgggttt gaaattttga atacttacaa
     2221 ttattcttct cgatcttcct ctctttcctt aaatcctgcg tacaaatccg tcgacgcaat
     2281 acattacaca gttgtcaatt ggttctcagc tctaccaaaa acatctattg ccaaaagaaa
     2341 ggtctatttg tacttcactg ttacagctga gaacattaaa tataataagc aaatttgata
     2401 aaacaaaggg ttctcacctt attccaaaag aatagtgtaa aatagggtaa tagagaaatg
     2461 ttaataaaag gaaattaaaa atagatattt tggttggttc agattttgtt tcgtagatct
     2521 acagggaaat ctccgccgtc aatgcaaagc gaaggtgaca cttggggaag gaccagtggt
     2581 ccgtacaatg ttacttaccc atttctcttc acgagacgtc gataatcaaa ttgtttattt
     2641 tcatattttt aagtccgcag ttttattaaa aaatcatgga cccgacatta gtacgagata
     2701 taccaatgag aagtcgacac gcaaatccta aagaaaccac tgtggttttt gcaaacaaga
     2761 gaaaccagct ttagcttttc cctaaaacca ctcttaccca aatctctcca taaataaaga
     2821 tcccgagact caaacacaag tctttttata aaggaaagaa agaaaaactt tcctaattgg
     2881 ttcataccaa agtctgagct cttctttata tctctcttgt agtttcttat tgggggtctt
     2941 tgttttgttt ggttctttta gagtaagaag tttcttaaaa aaggatcaaa aatggaattc
     3001 gagctcgccc ggggatcctc tagagtcagc ttccgctcaa ttggctgcgc tggcgctagc
     3061 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga
     3121 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
     3181 gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc
     3241 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
     3301 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
     3361 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt
     3421 tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
     3481 aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga
     3541 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
     3601 tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga
     3661 tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg
     3721 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
     3781 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
     3841 cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac
     3901 aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg
     3961 attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct
     4021 aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat
     4081 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
     4141 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
     4201 acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt
     4261 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
     4321 ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct
     4381 ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa
     4441 caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt
     4501 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
     4561 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
     4621 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
     4681 aaggccaaga agggcggaaa gatcgccgtg taagcggccg cggccggccc gggcgcgcct
     4741 gatcaatcga tgtttaaact taaaacagct ctggggttgt acccacccca gaggcccacg
     4801 tggcggctag tactccggta ttgcggtacc cttgtacgcc tgttttatac tcccttcccg
     4861 taacttagac gcgcaaaacc aagttcaata gaagggggta caaaccagta ccaccacgaa
     4921 caagcacttc tgtttccccg gtgatgtcgt atagactgct tgcgtggttg aaagcgacgg
     4981 atccgttatc cgcttatgta cttcgagaag cccagtacca cctcggaatc ttcgatgcgt
     5041 tgcgctcagc actcaacccc agagtgtagc ttaggctgat gagtctggac atccctcacc
     5101 ggtgacggtg gtccaggctg cgttggcggc ctacctatgg ctaacgccat gggacgctag
     5161 ttgtgaacaa ggtgtgaaga gcctattgag ctacataaga atcctccggc ccctgaatgc
     5221 ggctaatccc aacctcggag caggtggtca caaaccagtg attggcctgt cgtaacgcgc
     5281 aagtccgtgg cggaaccgac tactttgggt gtccgtgttt ccttttattt tattgtggct
     5341 gcttatggtg acaatcacag attgttatca taaagcgaat tggattctcg agactagtgc
     5401 gatcgcttaa ttaatttaaa tacgcgttta aacagatcca gatcttatta aagcagaact
     5461 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5521 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     5581 atgtctggtc gactctagac tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     5641 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5701 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5761 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5821 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5881 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5941 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6001 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6061 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6121 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6181 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     6241 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6301 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6361 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6421 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6481 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6541 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6601 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6661 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6721 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6781 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6841 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6901 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6961 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7021 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7081 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7141 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7201 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7261 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7321 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7381 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7441 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7501 ttccgcgcac atttccccga aaagtgccac ctgacgtcgt cgacgatcga cctgcag
//
