LOCUS       sequence-31111-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Paprotka K, Giese B, Fraunholz MJ
  TITLE     Codon-improved fluorescent proteins in investigation of 
            Staphylococcus aureus host pathogen interactions.
  JOURNAL   J Microbiol Methods. 2010 Oct;83(1):82-6. doi: 
            10.1016/j.mimet.2010.07.022. Epub 2010 Aug 11.
  PUBMED    20708040
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.mimet.2010.07.022"; journalName: "J Microbiol Methods"; 
            date: "2010-10"; volume: "83"; issue: "1"; pages: "82-6"
COMMENT     Sequence Label: pCerulean
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    6..24
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    91..109
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 ttgacactct atcattgata gagttatttg tcaaactagt tttttatttg gatcccctcg
       61 agttcatgaa aaactaaaaa aaatattgac actctatcat tgatagagta taattaaaat
      121 aagcttgatg gtaccagatc ctaggaaagg aggatgataa aatggtttca aaaggtgaag
      181 aattattcac tggagtagta cctatcttag tagaattaga tggtgatgta aatggtcata
      241 aattctcagt atcaggtgaa ggtgaaggtg atgctacata tggtaaatta acattaaaat
      301 tcatctgtac aacaggtaaa ttacctgtac cttggccaac attagtaaca acattaacat
      361 ggggagtaca atgtttcgca agatatcctg atcatatgaa acaacatgat ttcttcaaat
      421 cagcaatgcc tgaaggttac gtacaagaaa gaacaatctt cttcaaagat gatggtaatt
      481 ataaaacaag agctgaagta aaattcgaag gtgatacatt agtaaataga atcgagttaa
      541 aaggtatcga tttcaaagaa gatggtaata tcttaggtca taaattagaa tataatgcta
      601 tttcagataa tgtatatatc acagctgata aacaaaaaaa tggtatcaaa gcaaatttca
      661 aaatcagaca taatatcgaa gacggttcag tacaattagc agatcattat caacaaaata
      721 cacctatcgg tgatggtcct gtattattac ctgataatca ttacttaagt acacaatcag
      781 ctttatcaaa agatcctaat gaaaaaagag atcatatggt attattagaa tttgtaacag
      841 ctgctggtat cacacttggt atggatgaat tatataataa ata
//
