LOCUS       sequence-311118-        6991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for expression of FLuc controlled by the blue 
            light-repressible system .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Ochoa-Fernandez R, Abel NB, Wieland FG, Schlegel J, Koch LA, Miller 
            JB, Engesser R, Giuriani G, Brandl SM, Timmer J, Weber W, Ott T, 
            Simon R, Zurbriggen MD
  TITLE     Optogenetic control of gene expression in plants in the presence of 
            ambient white light.
  JOURNAL   Nat Methods. 2020 Jul;17(7):717-725. doi: 10.1038/s41592-020-0868-y.
            Epub 2020 Jun 29.
  PUBMED    32601426
REFERENCE   2  (bases 1 to 6991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0868-y"; journalName: "Nat Methods"; date: 
            "2020-07"; volume: "17"; issue: "7"; pages: "717-725"
COMMENT     Sequence Label: pROF402
FEATURES             Location/Qualifiers
     source          1..6991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     156..175
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    346..370
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(620..2272)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     2188..2206
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(2342..2366)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3459..3481
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     rep_origin      3608..4043
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     primer_bind     complement(4323..4342)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4334..5149
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             4334..5149
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4407..4426)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(6658..6991,1..255)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
       61 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      121 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      181 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      241 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      301 ccttttgctc acatgagatc tcaaacaaac acatacagcg acttagttta cccgccaata
      361 tatcctgtca agcttcgtct caagcgaggt cactggattt tggttttagg aattagaaat
      421 tttattgata gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa
      481 cacatgagcg aaaccctata agaaccctaa ttcccttatc tgggaactac tcacacatta
      541 ttctggagaa aaatagagag agatagattt gtagagagag actggtgatt tttgcggact
      601 ctagcatggc cgaagctcat tacacggcga tctttccgcc cttcttggcc tttatgagga
      661 tctctctgat ttttcttgcg tcgagttttc cggtaagacc tttcggtact tcgtccacaa
      721 acacaactcc tccgcgcaac tttttcgcgg ttgttacttg actggcgacg taatccacga
      781 tctctttttc cgtcatcgtc tttccgtgct ccaaaacaac aacggcggcg ggaagttcac
      841 cggcgtcatc gtcgggaaga cctgcgacac ctgcgtcgaa gatgttgggg tgttggagca
      901 agatggattc caattcagcg ggagccacct gatagccttt gtacttaatc agagacttca
      961 ggcggtcaac gatgaagaag tgttcgtctt cgtcccagta agctatgtct ccagaatgta
     1021 gccatccatc cttgtcaatc aaggcgttgg tcgcttccgg attgtttaca taaccggaca
     1081 taatcatagg acctctcaca cacagttcgc ctctttgatt aacgcccagc gttttcccgg
     1141 tatccagatc cacaaccttc gcttcaaaaa atggaacaac tttaccgacc gcgcccggtt
     1201 tatcatcccc ctcgggtgta atcagaatag ctgatgtagt ctcagtgagc ccatatcctt
     1261 gcctgatacc tggcagatgg aacctcttgg caaccgcttc cccgacttcc ttagagaggg
     1321 gagcgccacc agaagcaatt tcgtgtaaat tagataaatc gtatttgtca atcagagtgc
     1381 ttttggcgaa gaaggagaat agggttggca ccagcagcgc actttgaatc ttgtaatcct
     1441 gaaggctcct cagaaacagc tcttcttcaa atctatacat taagacgact cgaaatccac
     1501 atatcaaata tccgagtgta gtaaacattc caaaaccgtg atggaatgga acaacactta
     1561 aaatcgcagt atccggaatg atttgattgc caaaaatagg atctctggca tgcgagaatc
     1621 tcacgcaggc agttctatga ggcagagcga cacctttagg cagaccagta gatccagagg
     1681 agttcatgat cagtgcaatt gtcttgtccc tatcgaagga ctctggcaca aaatcgtatt
     1741 cattaaaacc gggaggtaga tgagatgtga cgaacgtgta catcgactga aatccctggt
     1801 aatccgtttt agaatccatg ataataattt tttggatgat tgggagcttt ttttgcacgt
     1861 tcaaaatttt ttgcaacccc tttttggaaa cgaacaccac ggtaggctgc gaaatgccca
     1921 tactgttgag caattcacgt tcattataaa tgtcgttcgc gggcgcaact gcaactccga
     1981 taaataacgc gcccaacacc ggcataaaga attgaagaga gttttcactg catacgacga
     2041 ttctgtgatt tgtattcagc ccatatcgtt tcatagcttc tgccaaccga acggacattt
     2101 cgaagtactc agcgtaagtg atgtccacct cgatatgtgc atctgtaaaa gcaattgttc
     2161 caggaaccag ggcgtatctc ttcatagcct tatgcagttg ctctccagcg gttccatctt
     2221 ccagcggata gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc attaccggta
     2281 ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg
     2341 cgatctgacg gttcactaaa cgagctctgc ttatataggg cggccgcggg aaagcttcat
     2401 ggactaaagg ctacctataa cgcatggact aaaggctacc tataacgcat ggactaaagg
     2461 ctacctataa cgcatggact aaaggctacc tataacgcat ggactaaagg ctacctactc
     2521 gaggctagcg tcaatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc
     2581 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
     2641 gtgggggtcc atctttggga ccactgtcgg caggggcatc ttgaacgata gcctttcctt
     2701 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
     2761 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
     2821 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
     2881 tgtcgtgctc caccatgttg acgaagattt tcttcttgtc attgagtcgt aaaagactct
     2941 gtatgaactg ttcgccagtc ttcacggcga gttctgttag atcctcgatc tgaatttttg
     3001 actccatggc ctttgattca gtaggaacta ctttcttaga gactccaatc tctattactt
     3061 gccttggttt atgaagcaag ccttgaatcg tccatactgg aatagtactt ctgatcttga
     3121 gaaatatatc tttctctgtg ttcttgatgc agttagtcct gaatcttttg actgcatctt
     3181 taaccttctt gggaaggtat ttgatctcct ggagattatt actcgggtag atcgtcttga
     3241 tcagacctgc cgcgtaggcc tctctaacca tctgtgggtc agcattcttt ctgaaattga
     3301 agaggctaat cttctcatta tcggtggtga acatggtatc gtcaccttct ccgtcgaact
     3361 ttcttcctag atcgtagaga tagagaaagt cgtccatggt gatctccggg gcaaaggaga
     3421 tcagcttggc tctagtctcc tgactgagac gaagcttgtt acaccacaat atatcctgcc
     3481 aagatctaat tccggggatc ggaaatccag aagcccgaga ggttgccgcc tttcgggctt
     3541 tttctttttc aaaaaaaaaa atttataaaa cgatctgttg cggccggccg ccgggttgtg
     3601 ggcaaaggcg ctcgacggtg ggcaaccgct tgcggttgtc cacgggcgga gccggtgcgc
     3661 gtagcgcatt gtccacaagc caagggcgac caataattga tatatatatt cataattgaa
     3721 aagctaattg aacatactac ttgctgtaac tacttgccgg agcgaggggt gtttgcaagc
     3781 tgttgatctg aaagggctat tagcgttctc acgtgccttt ttgattagcg atttcacgtg
     3841 accttattag cgatttcacg tactccgatt agcgatttca cgtaccctga ttagcgattt
     3901 cacgtggata gtttttggag cgggccggaa agccccgtga atcaaggctt tgcggggcat
     3961 tagcggtttc acgtggataa ctaccctcta tccacaggct tccggggata aaaaagcccg
     4021 ctcgacggcg ggctgttgga tggggatcgc ctgaatcgcc ccatcatcca gccagaaagt
     4081 gagggagcca cggttgatga gagctttgtt gtaggtggac cagttggtga ttttgaactt
     4141 ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc
     4201 agcaaaagtt cgatttattc aacaaagcca cgttgtgtct caaaatctct gatgttacat
     4261 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     4321 tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctcaaggc cgcgattaaa
     4381 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     4441 aggtgcgaca atctaccgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     4501 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     4561 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     4621 actcaccact gcgatcccag ggaaaacagc attccaggta ttagaagaat atcctgattc
     4681 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     4741 ttgtaattgt ccttttaaca gcgatcgcgt atttcgactc gctcaggcgc aatcacgaat
     4801 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     4861 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     4921 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     4981 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     5041 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     5101 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cactagacca
     5161 atgttacaca tatatacttt agactgatga atcccctaat gattttggta aaaatcatta
     5221 agttaaggtg gatacacatc ttgtcatatg atcaaatggt ttcgcgaaaa atcaataatc
     5281 agacaacaag atgtgcgaac tcgatatttt acacgactct ctttaccaat tctgccccga
     5341 attacactta aaacgactca acagcttaac gttggcttgc cacgcattac ttgactgtaa
     5401 aactctcact cttaccgaac ttggccgtaa cctgccaacc aaagcgagaa caaaacataa
     5461 catcaaacga atcgaccgat tgttaggtaa tcgtcacctc cacaaagagc gactcgctgt
     5521 ataccgttgg catgctagct ttatctgttc gggcaatacg atgcccattg tacttgttga
     5581 ctggtctgat attcgtgagc aaaaacgact tatggtattg cgagcttcag tcgcactaca
     5641 cggtcgttct gttactcttt atgagaaagc gttcccgctt tcagagcaat gttcaaagaa
     5701 agctcatgac caatttctag ccgaccttgc gagcattcta ccgagtaaca ccacaccgct
     5761 cattgtcagt gatgctggct ttaaagtgcc atggtataaa tccgttgaga agctgggttg
     5821 gtactggtta agtcgagtaa gaggaaaagt acaatatgca gacctaggag cggaaaactg
     5881 gaaacctatc agcaacttac atgatatgtc atctagtcac tcaaagactt taggctataa
     5941 gaggctgact aaaagcaatc caatctcatg ccaaattcta ttgtataaat ctcgctctaa
     6001 aggccgaaaa aatcagcgct cgacacggac tcattgtcac cacccgtcac ctaaaatcta
     6061 ctcagcgtcg gcaaaggagc catgggttct agcaactaac ttacctgttg aaattcgaac
     6121 acccaaacaa cttgttaata tctattcgaa gcgaatgcag attgaagaaa ccttccgaga
     6181 cttgaaaagt cctgcctacg gactaggcct acgccatagc cgaacgagca gctcagagcg
     6241 ttttgatatc atgctgctaa tcgccctgat gcttcaacta acatgttggc ttgcgggcgt
     6301 tcatgctcag aaacaaggtt gggacaagca cttccaggct aacacagtca gaaatcgaaa
     6361 cgtactctca acagttcgct taggcatgga agttttgcgg cattctggct acacaataac
     6421 aagggaagac ttactcgtgg ctgcaaccct actagctcaa aatttattca cacatggtta
     6481 cgctttgggg aaattatgag gggatctctc agtactttag attgatttaa aacttcattt
     6541 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6601 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6661 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6721 ggtggtttgt ttgccggatc aagagctacc aactcttctt ccgaaggtaa ctggcttcag
     6781 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6841 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6901 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6961 gcagcggtcg ggctgaacgg ggggttcgtg c
//
