LOCUS       sequence-311120-       14244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Venus under CCK gene promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14244)
  AUTHORS   Nagai Y, Nishitani N, Yasuda M, Ueda Y, Fukui Y, Andoh C, Shirakawa 
            H, Nakagawa T, Inoue KI, Nagayasu K, Kasparov S, Nakamura K, Kaneko 
            S
  TITLE     Identification of neuron-type specific promoters in monkey genome 
            and their functional validation in mice.
  JOURNAL   Biochem Biophys Res Commun. 2019 Oct 22;518(4):619-624. doi: 
            10.1016/j.bbrc.2019.08.101. Epub 2019 Aug 23.
  PUBMED    31451217
REFERENCE   2  (bases 1 to 14244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2019.08.101"; journalName: "Biochem Biophys Res 
            Commun"; date: "2019-10-22- 22"; volume: "518"; issue: "4"; pages: 
            "619-624"
COMMENT     Sequence Label: pTYF-super-mfCCK-3.9k-Venus-WPRE
FEATURES             Location/Qualifiers
     source          1..14244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(2679..2681,2682..2684,2685..3398)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2724..2745)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2985..3004)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3332..3353
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(3427..4001,4002..4004,4005..4013)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3594..3611)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3821..3840
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4014..4454
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             4479..5021
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     misc_feature    5105..5693
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5158..5178)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5576..5587)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5898..6122
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6255..6274)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(7296..7313)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7467..8055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7547..7566)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8226..9017,9018..9086))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8849..8868
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9087..9191)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9259..9277
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9315..9337)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9437..9456
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10718..10738
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10781..10961
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12343..12576
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    12838..12955
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    13068..13162
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 ccttagcagg ctcgtgggga aaccactgga aatcatttaa tcagctcttt caaacatatt
       61 gcccataagt tgcagaaacg cagctgcctc tcaggattag aaggtggagc agcaaccgga
      121 ggtgtctccg ggccgccccc cgccccacct tccctacccc cgcctcaccc actcgacccc
      181 cacactgtca tcgccacagt ccctccattc cagagggaat tccagagcgg aatacggatt
      241 ccgattttaa aataagcgct tctaaatctt tctccttccc tctaagacga atgagggaaa
      301 gaaagggaga ggagttgagg tcccaaggtt cagggatgcc tggtgatctg caggtcccag
      361 gctgggctgc ctcggtgcac ggagagggcg aggggcgcat tgtggagcct tggcaagcgc
      421 tgctcttcct cgatcctcgg tttgcggtct gtgaaatggg gttcgcagtg cctttgcggg
      481 gacgtcgcga actcaagcac cagcaagccg attcgaactc aaagggcttc agggtgtaga
      541 cgaccaccgc gcactgaggg actacgggtc ggcccgctcc ggagccagga gagtaaccgc
      601 gcgcagggaa aggcgcactg cagccttggt gcctcccttc cctccaccgg acgggtgagt
      661 gacgacgggc gaggtcctca tctccaggct gcgcagctga gttctggcag gtacggtcct
      721 aggggtgtga gtgtgtgggc cctccggaaa ggtgggaggg gcgaatgccg tggtctaccc
      781 aagcttgcag gggtgcggtg ggcaagttcc ccaggaccct cgctggcgcc gcttgtcggc
      841 agctgggaac agtgggcgcc gcgggtggcg gtaagttggc cagggaagga aacacatcgc
      901 tttcccaagc cggattcctc tgagcaaagc cagatggtgt catccccatt ttacagctgc
      961 gtaaagaaag gcccagaaca ccaacagcca gctgtggccg gagccctttc actgacccgg
     1021 aacactagct ctcagaaacc tggagaactg cgagggcttt taatgcggct gagaagaaag
     1081 taaagacccc gactcctctc tccttcgaaa gagcccacac aagtatgaag accagcgcct
     1141 cggacgctcc ggggtagggg ggcccactcc ccatccctct cagaatcgcg ccttactgac
     1201 gctcagtccc gcccagtgtc caccccccac ccccgcccca gtttcctttc tggaccctcc
     1261 ggtccccaaa gcgaactaag ctagcggcag cttctccatc ccagggaaga ctccaaactc
     1321 ctaggtttcc caccttggag agcactgctc tggaatgcgc aggggagcgg cagctggaaa
     1381 gggttggaag gaagcttctc ggacccagag gggggcagac accgggccca gccagggagg
     1441 ggttaaatcc gccccactgg accacctccc cctctccccc aggagccact tcaaccggtt
     1501 gtcgtcccag tggccaccct ctgagcacgt gttgctgcca gtctgcgtca gcgctgggta
     1561 aatacatgac tgggcgacgc cgccgggcgg ggctatttaa gaggcagccg ccctctggtc
     1621 ctccctgaac ttggctcagc tgccgggctg ctccggttgg aaacgccaag ccagctgcgt
     1681 tctaatccaa aaggtaggtc cctcggttcg tcagcggggg atccaagtgt cgtgtgtctt
     1741 tgaagaatgc tgtcgtcagc tttgtgccca tttccatttt gcttttctat tttctgtgtc
     1801 tttcctccga gagctcgaaa gacatttttt gaagtcagaa acaacggttc cgggtctaaa
     1861 agtgaggtct gggtaggtag agctgcttta gaggctttca cgcgccggca tacccattcg
     1921 ctaagattcg catgcagatt gagatttgct agctgttaca cagcggtgct ggggacgttg
     1981 caatattttc taataaaata ccagagggtt ttaagattca aattcatttt ctaaatactg
     2041 cagaaaattt cgaggcttta cggatagaaa tgtgagggtg ttttatccac tagcgatcta
     2101 aaatgcacgg ggctttggaa gcctgagtgc tgggcacata tttctgattt taaaacctgc
     2161 ctatgttttg cgtagtggct tattgccatt tcccacctac actttgagaa aactttataa
     2221 atacggcttt atttattgct cctacatttt aaactgtgct tctctttttc gtcatttact
     2281 gtatttccat attgaaagct tgttagctat ctggaaaggt gctttcccta ggcttctcat
     2341 ttctcccacg gagtcttccc tttaggatat ttttttcaca ctatttttaa tctcgtgggg
     2401 ttttattttt cttctttcag ttagaagaga ggcactctgc aaacacttcc tcaaacttta
     2461 gggaagcaga aaacattcct tctgctcaac acggcaaaaa acagggtctc tgtctgcccc
     2521 tcggcactgg gtgtggagtt cccaaagacc agccgtgtgc gcccgggggc gcgcgccatg
     2581 gcactcggct tgagccctcg tgcttgtggt ggcctgggga gtctggagca cctgtgtttg
     2641 tcctaagccg gtttctgggt ggcggccgcg gatccaccat ggtgagcaag ggcgaggagc
     2701 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     2761 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga
     2821 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg
     2881 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     2941 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     3001 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     3061 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     3121 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     3181 tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc
     3241 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc
     3301 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     3361 ccgggatcac tctcggcatg gacgagctgt acaagtaagg cgcgccctag tcggatatcc
     3421 gtcgagcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     3481 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     3541 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     3601 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     3661 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     3721 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     3781 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     3841 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     3901 tcggtacaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     3961 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatgaagc
     4021 tactgtcttc tatcgaacaa gcatgcgata tttgccgact taaaaagctc aagtgctcca
     4081 aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg ggagtgtcgc tactctccca
     4141 aaaccaaaag gtctccgctg actagggcac atctgacaga agtggaatca aggctagaaa
     4201 gactggaaca gctatttcta ctgatttttc ctcgagaaga ccttgacatg attttgaaaa
     4261 tggattcttt acaggatata aaagcattgt taacaggatt atttgtacaa gataatgtga
     4321 ataaagatgc cgtcacagat agattggctt cagtggagac tgatatgcct ctaacattga
     4381 gacagcatag aataagtgcg acatcatcat cggaagagag tagtaacaaa ggtcaaagac
     4441 agttgactgt atcgccggaa ttgggatcca tggtgtttcc ttctgggcag atctcaaacc
     4501 aggccctggc cttagcaccg tcctctgccc cagtccttgc ccagaccatg gtcccttcct
     4561 cagccatggt acctctggct cagcccccag ctcctgcccc agttctaacc ccgggtcctc
     4621 cccagtccct gtctgcacct gttccaaaga gcacccaggc tggggaaggc acgctgtcgg
     4681 aagccctgct gcacctgcag tttgatgctg atgaagactt gggggccttg cttggcaaca
     4741 gcacagaccc aggagtgttc acagacctgg catctgtgga caactcagag tttcagcagc
     4801 tcctgaacca gggtgtgtcc atgtctcact ccacagctga gcccatgctg atggagtacc
     4861 ctgaagctat aactcgcctg gtgacagggt cccagaggcc ccctgaccca gctcccacac
     4921 ccctggggac ctcggggctt cccaatggtc tctccggaga tgaagacttc tcctccattg
     4981 cggacatgga cttctctgct ctgctgagtc agatcagctc cagcggccaa tctagactgc
     5041 agctcgacct cgaggggggg cccggtacca ggtaagttaa catcgaggga tcaagcttat
     5101 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     5161 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     5221 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     5281 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     5341 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     5401 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     5461 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     5521 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     5581 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     5641 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgtcg
     5701 actagggata acagggtaac ggtaccttta agaccaatga cttacaaggc agctgtagat
     5761 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccagggtctc
     5821 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5881 agcctcataa agctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     5941 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     6001 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtaggg tggggcagga
     6061 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     6121 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     6181 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6241 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctg
     6301 tgcgggagaa cggagttcta ttatgactca aatcagtctc cccaagcatt cggggatcag
     6361 agtttttaag gataacttag tgtgtagggg gccagtgagt tggagatgaa agcgtaggga
     6421 gtcgaaggtg tccttttgcg ccgagtcagt tcctgggtgg gggccacaag atcggatgag
     6481 ccagtttatc aatccggggg tgccagctga tccatggagt gcagggtctg caaaatatct
     6541 caagcactga ttgatcttag gttttacaat agtgatgtta ccccaggaac aatttgggga
     6601 aggtcagaat cttgtagcct gtagctgcat gactcctaaa ccataatttc ttttttgttt
     6661 tttttttttt atttttgaga cagggtctca ctctgtcacc taggctggag tgcagtggtg
     6721 caatcacagc tcactgcagc ctcaacgtcg taagctcaag cgatcctccc acctcagcct
     6781 gcctggtagc tgagactaca agcgacgccc cagttaattt ttgtattttt ggtagaggca
     6841 gcgttttgcc gtgtggccct ggctggtctc gaactcctgg gctcaagtga tccagcctca
     6901 gcctcccaaa gtgctgggac aaccggggcc agtcactgca cctggcccta aaccataatt
     6961 tctaatcttt tggctaattt gttagtccta caaaggcagt ctagtcccca ggcaaaaagg
     7021 gggtttgttt cgggaaaggg ctgttactgt ctttgtttca aactataaac taagttcctc
     7081 ctaaacttag ttcggcctac acccaggaat gaacaaggag agcttggagg ttagaagcac
     7141 gatggaattg gttaggtcag atctctttca ctgtctgagt tataattttg caatggtggt
     7201 tcaaagactg cccgcttctg acaccagtcg ctgcattaat gaatcggcca acgcgcgggg
     7261 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7321 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7381 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7441 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7501 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7561 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7621 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7681 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7741 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7801 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7861 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7921 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7981 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8041 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8101 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8161 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8221 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8281 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8341 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8401 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8461 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8521 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8581 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8641 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8701 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8761 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8821 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8881 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8941 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9001 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9061 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9121 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9181 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     9241 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     9301 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     9361 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     9421 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     9481 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     9541 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccaggggagg
     9601 cagagattgc agtaagctga gatcgcagca ctgcactcca gcctgggcga cagagtaaga
     9661 ctctgtctca aaaataaaat aaataaatca atcagatatt ccaatctttt cctttattta
     9721 tttatttatt ttctattttg gaaacacagt ccttccttat tccagaatta cacatatatt
     9781 ctatttttct ttatatgctc cagttttttt tagaccttca cctgaaatgt gtgtatacaa
     9841 aatctaggcc agtccagcag agcctaaagg taaaaaataa aataataaaa aataaataaa
     9901 atctagctca ctccttcaca tcaaaatgga gatacagctg ttagcattaa ataccaaata
     9961 acccatcttg tcctcaataa ttttaagcgc ctctctccac cacatctaac tcctgtcaaa
    10021 ggcatgtgcc ccttccgggc gctctgctgt gctgccaacc aactggcatg tggactctgc
    10081 agggtcccta actgccaagc cccacagtgt gccctgaggc tgccccttcc ttctagcggc
    10141 tgcccccact cggctttgct ttccctagtt tcagttactt gcgttcagcc aaggtctgaa
    10201 actaggtgcg cacagagcgg taagactgcg agagaaagag accagcttta cagggggttt
    10261 atcacagtgc accctgacag tcgtcagcct cacagggggt ttatcacatt gcaccctgac
    10321 agtcgtcagc ctcacagggg gtttatcaca gtgcaccctt acaatcattc catttgattc
    10381 acaatttttt tagtctctac tgtgcctaac ttgtaagtta aatttgatca gaggtgtgtt
    10441 cccagagggg aaaacagtat atacagggtt cagtactatc gcatttcagg cccgagagct
    10501 gcatccggag tatctagatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
    10561 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
    10621 cgccaatagg gactttccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
    10681 gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta
    10741 ctctagaagg tctatataag cagagctcgt ttagtgaacc gggtctctct ggttagacca
    10801 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    10861 cttgccttga gtgctcaaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    10921 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
    10981 ttgaaagcga aagtaaagcc agaggagatc tctcgacgca ggactcggct tgctgaagcg
    11041 cgcacggcaa gaggcgaggg gcggcgactg cagagtacgc caaaaatttt gactagcgga
    11101 ggctagaagg agagagatgg gtgcgagagc gtcggtatta agcgggggag aattagataa
    11161 atgggaaaaa attcggttaa ggccaggggg aaagaaacaa tataaactaa aacatatagt
    11221 atgggcaagc agggagctag aacgattcgc agttaatcct ggccttttag agacatcaga
    11281 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
    11341 tagatcatta tataatacaa tagcagtcct ctattgtgtg catcaaagga tagatgtaaa
    11401 agacaccaag gaagccttag ataagataga ggaagagcaa aacaaaagta agaaaaaggc
    11461 acagcaagca gcagctgaca caggaaacaa cagccaggtc agccaaaatt accctatagt
    11521 gcagaacctc caggggcaaa tggtacatca ggccatatca cctagaactt taaatgcatg
    11581 ggtaaaagta gtagaagaga aggctttcag cccagaagta atacccatgt tttcagcatt
    11641 atcagaagga gccaccccac aagatttaaa taccatgcta aacacagtgg ggggacatca
    11701 agcagccatg caaatgttaa aagagaccat caatgaggaa gctgcagaat gggatagatt
    11761 gcatccagtg catgcagggc ctattgcacc aggccagatg agagaaccaa ggggaagtga
    11821 catagcagga actactagca aattaagaga acaatttgga aataataaaa caataatctt
    11881 taagcaatcc tcaggagggg acccagaaat tgtaacgcac agttttaatt gtggagggga
    11941 atttttctac tgtaattcaa cacaactgtt taatagtact tggtttaata gtacttggag
    12001 tactgaaggg tcaaataaca ctgaaggaag tgacacaatc acactcccat gcagaataaa
    12061 acaatttata aacatgtggc aggaagtagg aaaagcaatg tatgcccctc ccatcagtgg
    12121 acaaattaga tgttcatcaa atattactgg gctgctatta acaagagatg gtggtaataa
    12181 caacaatggg tccgagatct tcagacctgg aggaggcgat atgagggaca attggagaag
    12241 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
    12301 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
    12361 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
    12421 cagacaatta ttgtctgata tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
    12481 gcaacagcat ctgttgcaac tcacagtctg gggcatcaaa cagctccagg caagaatcct
    12541 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
    12601 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
    12661 gatttggaat aacatgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
    12721 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
    12781 ggaattagat aaatgggctt gcggccggaa ttcacaaatg gcagtattca tccacaattt
    12841 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
    12901 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
    12961 ttattacagg gacagcagag atccactttg ggcggcctag ggataacagg gtaatagatc
    13021 ttgctagctc ttatcatgtc tggatccaag cttgcatgcc tgcaggtcgg agtactgtcc
    13081 tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
    13141 gagcggagta ctgtcctccg agcggagact ctagagggtc ccgggatacg cgttctacct
    13201 tgattgctcc tgcttgtgca tccccgaggc cctcctaacc agccttcccc ttccagtcca
    13261 tctgcctttt gggcagtttc catatcacca cctagctcgc ctgaccaaaa catttctcca
    13321 agatttcctc ctaccatctc cactccatag tccccaaata tcagaattgt tgggaggtgt
    13381 ttgtccactt gtttgcttct gctcccctat tgtattgtca gctcttgttc atactgaatc
    13441 cctggggccc aacacaaagt aagtgttgaa taaacatgaa ttacatggac caagggccgg
    13501 gagttgagta actggtataa ggtagggaat ttctatagaa atggtctcaa agtgtgcttg
    13561 gtggaccagc attttcagta tggccaggga acttgttaga aatgtggatt cttggtgggg
    13621 tgcagtggct cacacctata atcccagcat tttcggaggc tgaggtgggt ggatcacctg
    13681 aggtcagaag ttcgagacca acctggccac tggggaggct gaggcaggag aatcttttga
    13741 acccaggagg cagaggttgc agtgagccga gatgacgcca ctgtactcca gcctgggtga
    13801 cagaatgaga ctctgtctga acgaaggaag gaaaagaaag aaagaaagaa agacagaaag
    13861 agagagagag agagagaaag aaagaaagaa agaaaggaag gaaggaagga aggaaggaag
    13921 gagaaagaaa gagaaagaaa gagagagaga ggaaaagagg gaaagaggga tagaggaaaa
    13981 gggaaagaga gagaggaagg gagggaagga gggagggagg gaggaaagga ggaaggaagg
    14041 aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaaag
    14101 aaggaacgaa ttcagattat tgggcccttc ctcaagcccg ctgaattaga agttctgggg
    14161 atattttgat aacaaggtta tgttttcaaa tatgtcttgc cacagtcaca aataagacta
    14221 gttttcccaa ctgaactacg aaaa
//
