LOCUS       sequence-311121-       11666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Venus under vAChT gene promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11666)
  AUTHORS   Nagai Y, Nishitani N, Yasuda M, Ueda Y, Fukui Y, Andoh C, Shirakawa 
            H, Nakagawa T, Inoue KI, Nagayasu K, Kasparov S, Nakamura K, Kaneko 
            S
  TITLE     Identification of neuron-type specific promoters in monkey genome 
            and their functional validation in mice.
  JOURNAL   Biochem Biophys Res Commun. 2019 Oct 22;518(4):619-624. doi: 
            10.1016/j.bbrc.2019.08.101. Epub 2019 Aug 23.
  PUBMED    31451217
REFERENCE   2  (bases 1 to 11666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2019.08.101"; journalName: "Biochem Biophys Res 
            Commun"; date: "2019-10-22- 22"; volume: "518"; issue: "4"; pages: 
            "619-624"
COMMENT     Sequence Label: pTYF-super-mfvAChT-1.1k-Venus-WPRE
FEATURES             Location/Qualifiers
     source          1..11666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    496..613
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    726..820
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             join(2003..2005,2006..2008,2009..2722)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2048..2069)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2309..2328)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2656..2677
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2751..3325,3326..3328,3329..3337)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2918..2935)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3145..3164
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3338..3778
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3803..4345
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     misc_feature    4429..5017
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4482..4502)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4900..4911)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5222..5446
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5579..5598)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(6620..6637)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6791..7379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6871..6890)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7550..8341,8342..8410))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8173..8192
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8411..8515)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8583..8601
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8639..8661)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8761..8780
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10042..10062
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10105..10285
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
       61 gacgctgacg gtacaggcca gacaattatt gtctgatata gtgcagcagc agaacaattt
      121 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaaaca
      181 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
      241 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
      301 taataaatct ctggaacaga tttggaataa catgacctgg atggagtggg acagagaaat
      361 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
      421 gaatgaacaa gaattattgg aattagataa atgggcttgc ggccggaatt cacaaatggc
      481 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
      541 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
      601 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggg cggcctaggg
      661 ataacagggt aatagatctt gctagctctt atcatgtctg gatccaagct tgcatgcctg
      721 caggtcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
      781 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagactct agagggtccc
      841 gggatacgcg tgggggcgag gcaacttctg ccctcagtcc ccacaagctc ccggccggga
      901 ttcgctgcag agaaattctg cagaggggcc ggtgggtcag agccaggcca ggcagttgct
      961 gagccttccc ctcccctccc ggcggggtgt gtgtgcaagg ggccaggggg cggagtcgga
     1021 gaaagggttg ggggtgtccg tgtccagcgc tcctccggat gccgctccac aatccagctg
     1081 cgaaaacaga tggacgcagc ggcggctcac ctcgggggct tcctgcccgc tgtgcgccga
     1141 ggtccaggct gaggcggaga tcgcaagcct ccggctcccg cctctctcga ggggccgcga
     1201 ggaacaggct gccgcgcctc cctccccagg agaagagtgg gcgctgaccc cgcgggtcct
     1261 agaaccgcgg gcggggggcg ggcaggggga gggcagaggg tcggctccgg gcgcggaaca
     1321 cccatcccag gggctggttc aggacttccg caccgagacc ccttcctggg ccttcagccc
     1381 tggagcatct ggggagcgga cccctgtcct gccacgcccc gcccccggcc cccgccccgc
     1441 cgacgtcgca ttagcatgag cgacgcaagt ggcccgggca ccactcgggg gctgggactc
     1501 gccgcgtcac agccccgagt ggaagggaaa aaaaaaagag gaggcggcgg aggaggcaag
     1561 agccgacgcg aggggagggg agcgcggcgg cggggctaac gggcgggcaa ccgggcgggc
     1621 ggcaacagca tgcccctcgg ccagcgcggg cggcctctca gcgcggtggg ggctgctctg
     1681 ggcgcgctca gggcacagtg cgcccagtct ccggccccgg cccctcgtcg cgcccgcctt
     1741 cccggccgcc cctgagccca gcagccgcgg gtcccgggaa ccgctaagag tagcggcaac
     1801 gcctagccgg acagagtcct ttcctttgcc gggacgctgg gtcatgagcc ccgcggccac
     1861 ctgaggcacg gggggtctgc tcggccagga ccgcctcccc gaagtaccgt gccctcgcct
     1921 ctgcactgcg ggacgccagc gctcggccct ggcggaggcg tcctcggaag agcatcgggg
     1981 tgggggcggc cgcggatcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     2041 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     2101 gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa
     2161 gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc
     2221 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     2281 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     2341 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     2401 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     2461 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     2521 ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     2581 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
     2641 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2701 catggacgag ctgtacaagt aaggcgcgcc ctagtcggat atccgtcgag cccctctccc
     2761 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     2821 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     2881 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     2941 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     3001 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     3061 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     3121 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     3181 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt
     3241 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     3301 ttcctttgaa aaacacgatg ataatatggc cacaaccatg aagctactgt cttctatcga
     3361 acaagcatgc gatatttgcc gacttaaaaa gctcaagtgc tccaaagaaa aaccgaagtg
     3421 cgccaagtgt ctgaagaaca actgggagtg tcgctactct cccaaaacca aaaggtctcc
     3481 gctgactagg gcacatctga cagaagtgga atcaaggcta gaaagactgg aacagctatt
     3541 tctactgatt tttcctcgag aagaccttga catgattttg aaaatggatt ctttacagga
     3601 tataaaagca ttgttaacag gattatttgt acaagataat gtgaataaag atgccgtcac
     3661 agatagattg gcttcagtgg agactgatat gcctctaaca ttgagacagc atagaataag
     3721 tgcgacatca tcatcggaag agagtagtaa caaaggtcaa agacagttga ctgtatcgcc
     3781 ggaattggga tccatggtgt ttccttctgg gcagatctca aaccaggccc tggccttagc
     3841 accgtcctct gccccagtcc ttgcccagac catggtccct tcctcagcca tggtacctct
     3901 ggctcagccc ccagctcctg ccccagttct aaccccgggt cctccccagt ccctgtctgc
     3961 acctgttcca aagagcaccc aggctgggga aggcacgctg tcggaagccc tgctgcacct
     4021 gcagtttgat gctgatgaag acttgggggc cttgcttggc aacagcacag acccaggagt
     4081 gttcacagac ctggcatctg tggacaactc agagtttcag cagctcctga accagggtgt
     4141 gtccatgtct cactccacag ctgagcccat gctgatggag taccctgaag ctataactcg
     4201 cctggtgaca gggtcccaga ggccccctga cccagctccc acacccctgg ggacctcggg
     4261 gcttcccaat ggtctctccg gagatgaaga cttctcctcc attgcggaca tggacttctc
     4321 tgctctgctg agtcagatca gctccagcgg ccaatctaga ctgcagctcg acctcgaggg
     4381 ggggcccggt accaggtaag ttaacatcga gggatcaagc ttatcgataa tcaacctctg
     4441 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     4501 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     4561 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     4621 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     4681 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     4741 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4801 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     4861 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4921 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     4981 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gtcgactagg gataacaggg
     5041 taacggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5101 aagaaaaggg gggactggaa gggctaattc actcccaggg tctctctggt tagaccagat
     5161 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc ataaagctcg
     5221 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5281 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5341 ctgagtaggt gtcattctat tctggggggt agggtggggc aggacagcaa gggggaggat
     5401 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     5461 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     5521 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5581 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctgtgcggg agaacggagt
     5641 tctattatga ctcaaatcag tctccccaag cattcgggga tcagagtttt taaggataac
     5701 ttagtgtgta gggggccagt gagttggaga tgaaagcgta gggagtcgaa ggtgtccttt
     5761 tgcgccgagt cagttcctgg gtgggggcca caagatcgga tgagccagtt tatcaatccg
     5821 ggggtgccag ctgatccatg gagtgcaggg tctgcaaaat atctcaagca ctgattgatc
     5881 ttaggtttta caatagtgat gttaccccag gaacaatttg gggaaggtca gaatcttgta
     5941 gcctgtagct gcatgactcc taaaccataa tttctttttt gttttttttt ttttattttt
     6001 gagacagggt ctcactctgt cacctaggct ggagtgcagt ggtgcaatca cagctcactg
     6061 cagcctcaac gtcgtaagct caagcgatcc tcccacctca gcctgcctgg tagctgagac
     6121 tacaagcgac gccccagtta atttttgtat ttttggtaga ggcagcgttt tgccgtgtgg
     6181 ccctggctgg tctcgaactc ctgggctcaa gtgatccagc ctcagcctcc caaagtgctg
     6241 ggacaaccgg ggccagtcac tgcacctggc cctaaaccat aatttctaat cttttggcta
     6301 atttgttagt cctacaaagg cagtctagtc cccaggcaaa aagggggttt gtttcgggaa
     6361 agggctgtta ctgtctttgt ttcaaactat aaactaagtt cctcctaaac ttagttcggc
     6421 ctacacccag gaatgaacaa ggagagcttg gaggttagaa gcacgatgga attggttagg
     6481 tcagatctct ttcactgtct gagttataat tttgcaatgg tggttcaaag actgcccgct
     6541 tctgacacca gtcgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6601 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6661 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6721 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6781 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6841 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6901 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6961 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7021 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7081 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7141 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7201 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7261 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7321 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7381 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7441 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7501 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7561 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7621 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7681 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7741 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7801 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7861 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7921 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7981 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8041 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8101 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8161 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8221 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8281 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8341 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8401 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8461 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8521 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     8581 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     8641 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     8701 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     8761 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     8821 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     8881 aagggcgatc ggtgcgggcc tcttcgctat tacgccaggg gaggcagaga ttgcagtaag
     8941 ctgagatcgc agcactgcac tccagcctgg gcgacagagt aagactctgt ctcaaaaata
     9001 aaataaataa atcaatcaga tattccaatc ttttccttta tttatttatt tattttctat
     9061 tttggaaaca cagtccttcc ttattccaga attacacata tattctattt ttctttatat
     9121 gctccagttt tttttagacc ttcacctgaa atgtgtgtat acaaaatcta ggccagtcca
     9181 gcagagccta aaggtaaaaa ataaaataat aaaaaataaa taaaatctag ctcactcctt
     9241 cacatcaaaa tggagataca gctgttagca ttaaatacca aataacccat cttgtcctca
     9301 ataattttaa gcgcctctct ccaccacatc taactcctgt caaaggcatg tgccccttcc
     9361 gggcgctctg ctgtgctgcc aaccaactgg catgtggact ctgcagggtc cctaactgcc
     9421 aagccccaca gtgtgccctg aggctgcccc ttccttctag cggctgcccc cactcggctt
     9481 tgctttccct agtttcagtt acttgcgttc agccaaggtc tgaaactagg tgcgcacaga
     9541 gcggtaagac tgcgagagaa agagaccagc tttacagggg gtttatcaca gtgcaccctg
     9601 acagtcgtca gcctcacagg gggtttatca cattgcaccc tgacagtcgt cagcctcaca
     9661 gggggtttat cacagtgcac ccttacaatc attccatttg attcacaatt tttttagtct
     9721 ctactgtgcc taacttgtaa gttaaatttg atcagaggtg tgttcccaga ggggaaaaca
     9781 gtatatacag ggttcagtac tatcgcattt caggcccgag agctgcatcc ggagtatcta
     9841 gatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     9901 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     9961 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
    10021 gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtactctag aaggtctata
    10081 taagcagagc tcgtttagtg aaccgggtct ctctggttag accagatctg agcctgggag
    10141 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctc
    10201 aaagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
    10261 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaagtaa
    10321 agccagagga gatctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
    10381 aggggcggcg actgcagagt acgccaaaaa ttttgactag cggaggctag aaggagagag
    10441 atgggtgcga gagcgtcggt attaagcggg ggagaattag ataaatggga aaaaattcgg
    10501 ttaaggccag ggggaaagaa acaatataaa ctaaaacata tagtatgggc aagcagggag
    10561 ctagaacgat tcgcagttaa tcctggcctt ttagagacat cagaaggctg tagacaaata
    10621 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
    10681 acaatagcag tcctctattg tgtgcatcaa aggatagatg taaaagacac caaggaagcc
    10741 ttagataaga tagaggaaga gcaaaacaaa agtaagaaaa aggcacagca agcagcagct
    10801 gacacaggaa acaacagcca ggtcagccaa aattacccta tagtgcagaa cctccagggg
    10861 caaatggtac atcaggccat atcacctaga actttaaatg catgggtaaa agtagtagaa
    10921 gagaaggctt tcagcccaga agtaataccc atgttttcag cattatcaga aggagccacc
    10981 ccacaagatt taaataccat gctaaacaca gtggggggac atcaagcagc catgcaaatg
    11041 ttaaaagaga ccatcaatga ggaagctgca gaatgggata gattgcatcc agtgcatgca
    11101 gggcctattg caccaggcca gatgagagaa ccaaggggaa gtgacatagc aggaactact
    11161 agcaaattaa gagaacaatt tggaaataat aaaacaataa tctttaagca atcctcagga
    11221 ggggacccag aaattgtaac gcacagtttt aattgtggag gggaattttt ctactgtaat
    11281 tcaacacaac tgtttaatag tacttggttt aatagtactt ggagtactga agggtcaaat
    11341 aacactgaag gaagtgacac aatcacactc ccatgcagaa taaaacaatt tataaacatg
    11401 tggcaggaag taggaaaagc aatgtatgcc cctcccatca gtggacaaat tagatgttca
    11461 tcaaatatta ctgggctgct attaacaaga gatggtggta ataacaacaa tgggtccgag
    11521 atcttcagac ctggaggagg cgatatgagg gacaattgga gaagtgaatt atataaatat
    11581 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
    11641 cagagagaaa aaagagcagt gggaat
//
