LOCUS       sequence-311123-       11309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Venus under PV gene promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11309)
  AUTHORS   Nagai Y, Nishitani N, Yasuda M, Ueda Y, Fukui Y, Andoh C, Shirakawa 
            H, Nakagawa T, Inoue KI, Nagayasu K, Kasparov S, Nakamura K, Kaneko 
            S
  TITLE     Identification of neuron-type specific promoters in monkey genome 
            and their functional validation in mice.
  JOURNAL   Biochem Biophys Res Commun. 2019 Oct 22;518(4):619-624. doi: 
            10.1016/j.bbrc.2019.08.101. Epub 2019 Aug 23.
  PUBMED    31451217
REFERENCE   2  (bases 1 to 11309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2019.08.101"; journalName: "Biochem Biophys Res 
            Commun"; date: "2019-10-22- 22"; volume: "518"; issue: "4"; pages: 
            "619-624"
COMMENT     Sequence Label: pTYF-super-mfPV-0.8k-Venus-WPRE
FEATURES             Location/Qualifiers
     source          1..11309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1563..1796
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2288..2382
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             join(3208..3210,3211..3213,3214..3927)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3253..3274)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3514..3533)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3861..3882
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(3956..4530,4531..4533,4534..4542)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4123..4140)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4350..4369
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4543..4983
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             5008..5550
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     misc_feature    5634..6222
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5687..5707)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6105..6116)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6427..6651
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6784..6803)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(7825..7842)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7996..8584)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8076..8095)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8755..9546,9547..9615))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9378..9397
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9616..9720)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9788..9806
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9844..9866)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9966..9985
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11247..11267
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgctcaaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagtaaagcc agaggagatc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg cagagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcggtatta
      361 agcgggggag aattagataa atgggaaaaa attcggttaa ggccaggggg aaagaaacaa
      421 tataaactaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
      481 ggccttttag agacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
      541 cagacaggat cagaagaact tagatcatta tataatacaa tagcagtcct ctattgtgtg
      601 catcaaagga tagatgtaaa agacaccaag gaagccttag ataagataga ggaagagcaa
      661 aacaaaagta agaaaaaggc acagcaagca gcagctgaca caggaaacaa cagccaggtc
      721 agccaaaatt accctatagt gcagaacctc caggggcaaa tggtacatca ggccatatca
      781 cctagaactt taaatgcatg ggtaaaagta gtagaagaga aggctttcag cccagaagta
      841 atacccatgt tttcagcatt atcagaagga gccaccccac aagatttaaa taccatgcta
      901 aacacagtgg ggggacatca agcagccatg caaatgttaa aagagaccat caatgaggaa
      961 gctgcagaat gggatagatt gcatccagtg catgcagggc ctattgcacc aggccagatg
     1021 agagaaccaa ggggaagtga catagcagga actactagca aattaagaga acaatttgga
     1081 aataataaaa caataatctt taagcaatcc tcaggagggg acccagaaat tgtaacgcac
     1141 agttttaatt gtggagggga atttttctac tgtaattcaa cacaactgtt taatagtact
     1201 tggtttaata gtacttggag tactgaaggg tcaaataaca ctgaaggaag tgacacaatc
     1261 acactcccat gcagaataaa acaatttata aacatgtggc aggaagtagg aaaagcaatg
     1321 tatgcccctc ccatcagtgg acaaattaga tgttcatcaa atattactgg gctgctatta
     1381 acaagagatg gtggtaataa caacaatggg tccgagatct tcagacctgg aggaggcgat
     1441 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1501 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1561 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1621 atgacgctga cggtacaggc cagacaatta ttgtctgata tagtgcagca gcagaacaat
     1681 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaaa
     1741 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1801 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1861 agtaataaat ctctggaaca gatttggaat aacatgacct ggatggagtg ggacagagaa
     1921 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1981 aagaatgaac aagaattatt ggaattagat aaatgggctt gcggccggaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg ggcggcctag
     2221 ggataacagg gtaatagatc ttgctagctc ttatcatgtc tggatccaag cttgcatgcc
     2281 tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
     2341 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagact ctagagggtc
     2401 ccgggatacg cgtgagacca ggaaagcaag tagttagtag agatctgaga agtgttcttt
     2461 gaaaaatcaa gccaacaaaa aaatgaaaat aaaacaaatc aggttttaga atgtgcatcc
     2521 ctggccagat gtgggatggg gcacccgaag tgggccccag cagctactgc ttgtccctgt
     2581 ccctgtccct gccacctcct ccctacgtcc gtgggtcagg tttcacgacc tcagcgctca
     2641 gattttgcag cataaatttg cgtccaggaa agaccagagc agaggctgaa gtaggagtgg
     2701 agagagaaag agagcaggtc ctagggggtc ctggggtgca ggctggccgc tgagggttca
     2761 ggatcttccg ccacgcggca ggacatcctc ggaggttccc agatctgaga ccccgcacct
     2821 cctgcgtgtc cttgacagca ggtggacttt ccagacctgg ctggtgactc cagtgccccc
     2881 taaatcccag aggtggcccc cttccccttg tgccctggcc gcagcccctg ggatcccgcg
     2941 gacccccggc ctggcggtgc aggagcggta gcaggtccgc gcgccctctt gcccgcggcc
     3001 ggcgctgctc agccgcgcga ctggactgag ccgctcccgc tgcgctcaga gtccgggccc
     3061 ctggcgccat gccaggcccg gcgggcgtgc gcccggcgcg cagcactgca gcgctggtca
     3121 tatgagcaga aatgatgaga aaagcacttt ttaatctttt cgcacttgct ctgcccgctc
     3181 aaacagttgc gcggccgcgg atccaccatg gtgagcaagg gcgaggagct gttcaccggg
     3241 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     3301 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc
     3361 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc
     3421 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     3481 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     3541 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     3601 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     3661 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
     3721 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     3781 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac
     3841 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     3901 ctcggcatgg acgagctgta caagtaaggc gcgccctagt cggatatccg tcgagcccct
     3961 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     4021 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     4081 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     4141 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     4201 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     4261 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     4321 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     4381 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtacacat
     4441 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     4501 tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatgaagct actgtcttct
     4561 atcgaacaag catgcgatat ttgccgactt aaaaagctca agtgctccaa agaaaaaccg
     4621 aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct actctcccaa aaccaaaagg
     4681 tctccgctga ctagggcaca tctgacagaa gtggaatcaa ggctagaaag actggaacag
     4741 ctatttctac tgatttttcc tcgagaagac cttgacatga ttttgaaaat ggattcttta
     4801 caggatataa aagcattgtt aacaggatta tttgtacaag ataatgtgaa taaagatgcc
     4861 gtcacagata gattggcttc agtggagact gatatgcctc taacattgag acagcataga
     4921 ataagtgcga catcatcatc ggaagagagt agtaacaaag gtcaaagaca gttgactgta
     4981 tcgccggaat tgggatccat ggtgtttcct tctgggcaga tctcaaacca ggccctggcc
     5041 ttagcaccgt cctctgcccc agtccttgcc cagaccatgg tcccttcctc agccatggta
     5101 cctctggctc agcccccagc tcctgcccca gttctaaccc cgggtcctcc ccagtccctg
     5161 tctgcacctg ttccaaagag cacccaggct ggggaaggca cgctgtcgga agccctgctg
     5221 cacctgcagt ttgatgctga tgaagacttg ggggccttgc ttggcaacag cacagaccca
     5281 ggagtgttca cagacctggc atctgtggac aactcagagt ttcagcagct cctgaaccag
     5341 ggtgtgtcca tgtctcactc cacagctgag cccatgctga tggagtaccc tgaagctata
     5401 actcgcctgg tgacagggtc ccagaggccc cctgacccag ctcccacacc cctggggacc
     5461 tcggggcttc ccaatggtct ctccggagat gaagacttct cctccattgc ggacatggac
     5521 ttctctgctc tgctgagtca gatcagctcc agcggccaat ctagactgca gctcgacctc
     5581 gagggggggc ccggtaccag gtaagttaac atcgagggat caagcttatc gataatcaac
     5641 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     5701 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     5761 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     5821 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     5881 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     5941 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6001 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     6061 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     6121 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     6181 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgtcga ctagggataa
     6241 cagggtaacg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6301 tttaaaagaa aaggggggac tggaagggct aattcactcc cagggtctct ctggttagac
     6361 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcataaa
     6421 gctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     6481 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     6541 attgtctgag taggtgtcat tctattctgg ggggtagggt ggggcaggac agcaaggggg
     6601 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     6661 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     6721 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6781 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggctgt gcgggagaac
     6841 ggagttctat tatgactcaa atcagtctcc ccaagcattc ggggatcaga gtttttaagg
     6901 ataacttagt gtgtaggggg ccagtgagtt ggagatgaaa gcgtagggag tcgaaggtgt
     6961 ccttttgcgc cgagtcagtt cctgggtggg ggccacaaga tcggatgagc cagtttatca
     7021 atccgggggt gccagctgat ccatggagtg cagggtctgc aaaatatctc aagcactgat
     7081 tgatcttagg ttttacaata gtgatgttac cccaggaaca atttggggaa ggtcagaatc
     7141 ttgtagcctg tagctgcatg actcctaaac cataatttct tttttgtttt ttttttttta
     7201 tttttgagac agggtctcac tctgtcacct aggctggagt gcagtggtgc aatcacagct
     7261 cactgcagcc tcaacgtcgt aagctcaagc gatcctccca cctcagcctg cctggtagct
     7321 gagactacaa gcgacgcccc agttaatttt tgtatttttg gtagaggcag cgttttgccg
     7381 tgtggccctg gctggtctcg aactcctggg ctcaagtgat ccagcctcag cctcccaaag
     7441 tgctgggaca accggggcca gtcactgcac ctggccctaa accataattt ctaatctttt
     7501 ggctaatttg ttagtcctac aaaggcagtc tagtccccag gcaaaaaggg ggtttgtttc
     7561 gggaaagggc tgttactgtc tttgtttcaa actataaact aagttcctcc taaacttagt
     7621 tcggcctaca cccaggaatg aacaaggaga gcttggaggt tagaagcacg atggaattgg
     7681 ttaggtcaga tctctttcac tgtctgagtt ataattttgc aatggtggtt caaagactgc
     7741 ccgcttctga caccagtcgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7801 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7861 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7921 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7981 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8041 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8101 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8161 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8221 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8281 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8341 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8401 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     8461 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8521 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8581 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8641 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8701 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8761 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8821 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8881 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8941 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9001 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9061 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9121 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9181 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9241 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9301 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9361 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9421 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9481 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9541 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9601 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9661 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9721 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     9781 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     9841 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     9901 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     9961 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    10021 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    10081 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc caggggaggc agagattgca
    10141 gtaagctgag atcgcagcac tgcactccag cctgggcgac agagtaagac tctgtctcaa
    10201 aaataaaata aataaatcaa tcagatattc caatcttttc ctttatttat ttatttattt
    10261 tctattttgg aaacacagtc cttccttatt ccagaattac acatatattc tatttttctt
    10321 tatatgctcc agtttttttt agaccttcac ctgaaatgtg tgtatacaaa atctaggcca
    10381 gtccagcaga gcctaaaggt aaaaaataaa ataataaaaa ataaataaaa tctagctcac
    10441 tccttcacat caaaatggag atacagctgt tagcattaaa taccaaataa cccatcttgt
    10501 cctcaataat tttaagcgcc tctctccacc acatctaact cctgtcaaag gcatgtgccc
    10561 cttccgggcg ctctgctgtg ctgccaacca actggcatgt ggactctgca gggtccctaa
    10621 ctgccaagcc ccacagtgtg ccctgaggct gccccttcct tctagcggct gcccccactc
    10681 ggctttgctt tccctagttt cagttacttg cgttcagcca aggtctgaaa ctaggtgcgc
    10741 acagagcggt aagactgcga gagaaagaga ccagctttac agggggttta tcacagtgca
    10801 ccctgacagt cgtcagcctc acagggggtt tatcacattg caccctgaca gtcgtcagcc
    10861 tcacaggggg tttatcacag tgcaccctta caatcattcc atttgattca caattttttt
    10921 agtctctact gtgcctaact tgtaagttaa atttgatcag aggtgtgttc ccagagggga
    10981 aaacagtata tacagggttc agtactatcg catttcaggc ccgagagctg catccggagt
    11041 atctagatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
    11101 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
    11161 actttccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
    11221 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac tctagaaggt
    11281 ctatataagc agagctcgtt tagtgaacc
//
