LOCUS       sequence-311135-       12803 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Venus under PENK gene promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12803)
  AUTHORS   Nagai Y, Nishitani N, Yasuda M, Ueda Y, Fukui Y, Andoh C, Shirakawa 
            H, Nakagawa T, Inoue KI, Nagayasu K, Kasparov S, Nakamura K, Kaneko 
            S
  TITLE     Identification of neuron-type specific promoters in monkey genome 
            and their functional validation in mice.
  JOURNAL   Biochem Biophys Res Commun. 2019 Oct 22;518(4):619-624. doi: 
            10.1016/j.bbrc.2019.08.101. Epub 2019 Aug 23.
  PUBMED    31451217
REFERENCE   2  (bases 1 to 12803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2019.08.101"; journalName: "Biochem Biophys Res 
            Commun"; date: "2019-10-22- 22"; volume: "518"; issue: "4"; pages: 
            "619-624"
COMMENT     Sequence Label: pTYF-super-mfPENK-2.2k-Venus-WPRE
FEATURES             Location/Qualifiers
     source          1..12803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     148..167
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             341..781
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             806..1348
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     misc_feature    1432..2020
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1485..1505)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1903..1914)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2225..2449
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2582..2601)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(3623..3640)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3794..4382)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3874..3893)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4553..5344,5345..5413))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5176..5195
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5414..5518)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5586..5604
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5642..5664)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5764..5783
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7045..7065
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7108..7288
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8670..8903
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9165..9282
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    9395..9489
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             join(11809..11811,11812..11814,11815..12528)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(11854..11875)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(12115..12134)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12462..12483
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(12557..12803,1..328,329..331,332..340)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(12724..12741)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
       61 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
      121 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
      181 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtacacatgc
      241 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
      301 gttttccttt gaaaaacacg atgataatat ggccacaacc atgaagctac tgtcttctat
      361 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa
      421 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc
      481 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct
      541 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca
      601 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt
      661 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat
      721 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc
      781 gccggaattg ggatccatgg tgtttccttc tgggcagatc tcaaaccagg ccctggcctt
      841 agcaccgtcc tctgccccag tccttgccca gaccatggtc ccttcctcag ccatggtacc
      901 tctggctcag cccccagctc ctgccccagt tctaaccccg ggtcctcccc agtccctgtc
      961 tgcacctgtt ccaaagagca cccaggctgg ggaaggcacg ctgtcggaag ccctgctgca
     1021 cctgcagttt gatgctgatg aagacttggg ggccttgctt ggcaacagca cagacccagg
     1081 agtgttcaca gacctggcat ctgtggacaa ctcagagttt cagcagctcc tgaaccaggg
     1141 tgtgtccatg tctcactcca cagctgagcc catgctgatg gagtaccctg aagctataac
     1201 tcgcctggtg acagggtccc agaggccccc tgacccagct cccacacccc tggggacctc
     1261 ggggcttccc aatggtctct ccggagatga agacttctcc tccattgcgg acatggactt
     1321 ctctgctctg ctgagtcaga tcagctccag cggccaatct agactgcagc tcgacctcga
     1381 gggggggccc ggtaccaggt aagttaacat cgagggatca agcttatcga taatcaacct
     1441 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     1501 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     1561 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     1621 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     1681 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     1741 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     1801 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     1861 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     1921 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     1981 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgtcgact agggataaca
     2041 gggtaacggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     2101 taaaagaaaa ggggggactg gaagggctaa ttcactccca gggtctctct ggttagacca
     2161 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcataaagc
     2221 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     2281 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     2341 tgtctgagta ggtgtcattc tattctgggg ggtagggtgg ggcaggacag caagggggag
     2401 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     2461 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     2521 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     2581 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctgtgc gggagaacgg
     2641 agttctatta tgactcaaat cagtctcccc aagcattcgg ggatcagagt ttttaaggat
     2701 aacttagtgt gtagggggcc agtgagttgg agatgaaagc gtagggagtc gaaggtgtcc
     2761 ttttgcgccg agtcagttcc tgggtggggg ccacaagatc ggatgagcca gtttatcaat
     2821 ccgggggtgc cagctgatcc atggagtgca gggtctgcaa aatatctcaa gcactgattg
     2881 atcttaggtt ttacaatagt gatgttaccc caggaacaat ttggggaagg tcagaatctt
     2941 gtagcctgta gctgcatgac tcctaaacca taatttcttt tttgtttttt tttttttatt
     3001 tttgagacag ggtctcactc tgtcacctag gctggagtgc agtggtgcaa tcacagctca
     3061 ctgcagcctc aacgtcgtaa gctcaagcga tcctcccacc tcagcctgcc tggtagctga
     3121 gactacaagc gacgccccag ttaatttttg tatttttggt agaggcagcg ttttgccgtg
     3181 tggccctggc tggtctcgaa ctcctgggct caagtgatcc agcctcagcc tcccaaagtg
     3241 ctgggacaac cggggccagt cactgcacct ggccctaaac cataatttct aatcttttgg
     3301 ctaatttgtt agtcctacaa aggcagtcta gtccccaggc aaaaaggggg tttgtttcgg
     3361 gaaagggctg ttactgtctt tgtttcaaac tataaactaa gttcctccta aacttagttc
     3421 ggcctacacc caggaatgaa caaggagagc ttggaggtta gaagcacgat ggaattggtt
     3481 aggtcagatc tctttcactg tctgagttat aattttgcaa tggtggttca aagactgccc
     3541 gcttctgaca ccagtcgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3601 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3661 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3721 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3781 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3841 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3901 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3961 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4021 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4081 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4141 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4201 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4261 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4321 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4381 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4441 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4501 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4561 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4621 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4681 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4741 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4801 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4861 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4921 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4981 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5041 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5101 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5161 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5221 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5281 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5341 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5401 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5461 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5521 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     5581 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     5641 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     5701 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     5761 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     5821 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     5881 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca ggggaggcag agattgcagt
     5941 aagctgagat cgcagcactg cactccagcc tgggcgacag agtaagactc tgtctcaaaa
     6001 ataaaataaa taaatcaatc agatattcca atcttttcct ttatttattt atttattttc
     6061 tattttggaa acacagtcct tccttattcc agaattacac atatattcta tttttcttta
     6121 tatgctccag ttttttttag accttcacct gaaatgtgtg tatacaaaat ctaggccagt
     6181 ccagcagagc ctaaaggtaa aaaataaaat aataaaaaat aaataaaatc tagctcactc
     6241 cttcacatca aaatggagat acagctgtta gcattaaata ccaaataacc catcttgtcc
     6301 tcaataattt taagcgcctc tctccaccac atctaactcc tgtcaaaggc atgtgcccct
     6361 tccgggcgct ctgctgtgct gccaaccaac tggcatgtgg actctgcagg gtccctaact
     6421 gccaagcccc acagtgtgcc ctgaggctgc cccttccttc tagcggctgc ccccactcgg
     6481 ctttgctttc cctagtttca gttacttgcg ttcagccaag gtctgaaact aggtgcgcac
     6541 agagcggtaa gactgcgaga gaaagagacc agctttacag ggggtttatc acagtgcacc
     6601 ctgacagtcg tcagcctcac agggggttta tcacattgca ccctgacagt cgtcagcctc
     6661 acagggggtt tatcacagtg cacccttaca atcattccat ttgattcaca atttttttag
     6721 tctctactgt gcctaacttg taagttaaat ttgatcagag gtgtgttccc agaggggaaa
     6781 acagtatata cagggttcag tactatcgca tttcaggccc gagagctgca tccggagtat
     6841 ctagatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     6901 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     6961 tttccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     7021 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtactc tagaaggtct
     7081 atataagcag agctcgttta gtgaaccggg tctctctggt tagaccagat ctgagcctgg
     7141 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7201 ctcaaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7261 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     7321 taaagccaga ggagatctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     7381 gcgaggggcg gcgactgcag agtacgccaa aaattttgac tagcggaggc tagaaggaga
     7441 gagatgggtg cgagagcgtc ggtattaagc gggggagaat tagataaatg ggaaaaaatt
     7501 cggttaaggc cagggggaaa gaaacaatat aaactaaaac atatagtatg ggcaagcagg
     7561 gagctagaac gattcgcagt taatcctggc cttttagaga catcagaagg ctgtagacaa
     7621 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     7681 aatacaatag cagtcctcta ttgtgtgcat caaaggatag atgtaaaaga caccaaggaa
     7741 gccttagata agatagagga agagcaaaac aaaagtaaga aaaaggcaca gcaagcagca
     7801 gctgacacag gaaacaacag ccaggtcagc caaaattacc ctatagtgca gaacctccag
     7861 gggcaaatgg tacatcaggc catatcacct agaactttaa atgcatgggt aaaagtagta
     7921 gaagagaagg ctttcagccc agaagtaata cccatgtttt cagcattatc agaaggagcc
     7981 accccacaag atttaaatac catgctaaac acagtggggg gacatcaagc agccatgcaa
     8041 atgttaaaag agaccatcaa tgaggaagct gcagaatggg atagattgca tccagtgcat
     8101 gcagggccta ttgcaccagg ccagatgaga gaaccaaggg gaagtgacat agcaggaact
     8161 actagcaaat taagagaaca atttggaaat aataaaacaa taatctttaa gcaatcctca
     8221 ggaggggacc cagaaattgt aacgcacagt tttaattgtg gaggggaatt tttctactgt
     8281 aattcaacac aactgtttaa tagtacttgg tttaatagta cttggagtac tgaagggtca
     8341 aataacactg aaggaagtga cacaatcaca ctcccatgca gaataaaaca atttataaac
     8401 atgtggcagg aagtaggaaa agcaatgtat gcccctccca tcagtggaca aattagatgt
     8461 tcatcaaata ttactgggct gctattaaca agagatggtg gtaataacaa caatgggtcc
     8521 gagatcttca gacctggagg aggcgatatg agggacaatt ggagaagtga attatataaa
     8581 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     8641 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     8701 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     8761 tctgatatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     8821 ttgcaactca cagtctgggg catcaaacag ctccaggcaa gaatcctggc tgtggaaaga
     8881 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     8941 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaataac
     9001 atgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     9061 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     9121 tgggcttgcg gccggaattc acaaatggca gtattcatcc acaattttaa aagaaaaggg
     9181 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     9241 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     9301 agcagagatc cactttgggc ggcctaggga taacagggta atagatcttg ctagctctta
     9361 tcatgtctgg atccaagctt gcatgcctgc aggtcggagt actgtcctcc gagcggagta
     9421 ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg
     9481 tcctccgagc ggagactcta gagggtcccg ggatacgcgt tcccgacctt gctactgtga
     9541 tgccccggcc gggaggcagg cggtgctcag gtacagctct ggtgctaatc cgggtgctgc
     9601 gtgtcaagcc cgcacagtga ggcctgtgga gaactgagac cccagtatcc gcaagctcag
     9661 cctacgcagg atagcgctgc cctctagcga ctataatggg acatgcagcg cggctgcagg
     9721 ttctagcgac ggtagaaatc cgccctttgc agagggcgca gagggccggg aaagccctgg
     9781 gaccttttcc ctatgtatgg gaccaggaac tgtttatggt ttccccctag gtctaggaga
     9841 gatagatgca taggtggatt ggatacatcc atggcagatg taaggtaagc agacaatggg
     9901 caccgatgga aaggtggacg acggataact gatggttagg aacacgtttt gagggcttgc
     9961 tatagtgcca ggcacaaggc taagagattt tgtccattat ttcatttgat tctctccaga
    10021 actttatgaa tggcatatta cctatgttcc tatttttcaa atagggaaac tgaggcctca
    10081 ggcaatgtaa gcagcttgcc acttagcaat cttttgcaga gccaggaagc gggaaagcgt
    10141 gtcttaatgg acagtctcag cctccacagt gtgccctcgg ccccctcccg gtggagatgt
    10201 tccaagccct ggcgtcccgg gtagggggtc cctcatcctt ccctccccac cacactcctg
    10261 gcgcgctgac attacacccg ccccggcacc cccttctcac tcatccaaca cccccaggaa
    10321 accctggaca gcgctctcaa ggcagtggat cttcgccttg ggagccctgg ggagctggat
    10381 aaacacggat cctctcccac agtggctgaa aagcgcgcag tgccggaacc tgagggttta
    10441 ccggcttcta cgcttggcca agggtctctc taactggaaa ggtgaaaatt ctgtgccgag
    10501 attttaagat tcccagaaag tttcaattgt taagtttctg taaccattaa ctgagcgcct
    10561 actgcgcacc ttgaagctgt tagatgctgc ggcaaggaac tcggagtcaa ctgtggggga
    10621 caagcgggtc gataaatgac gacattccga accagacggc tgtgcttggt gcccacgggg
    10681 accccgaggg ggtccaggga ggaggcggga aaggggcagg ttcatcggcc cgctgggtct
    10741 ccagcacatt ccagaagtct aagccagtcc atctatcctt ccaaacgcct ccacctcgct
    10801 tccctccttg gagcccgcac cccacggtgc aatttcagtg actttatgcg gagaaacttg
    10861 atcctatctc actctcccca aacttcctaa ctgccttggg tttgtcacct ggccgtgtcg
    10921 ggagccaccg agcgcccccc gtggccgtca ccctcggtgg gggaagcggc gcgcgggtac
    10981 tgggggaccg acccctcccg cgaaggcgtc ggcgcggggc tggcgtaggg cctgcgtcag
    11041 ctgcagcccg ccggcgattg gggagcgcgc gcctccttcg gtttggggct aattataaag
    11101 tggctccagc cgccgttaag ccccgggacg gcgaggcagg cgctcagagc cccgcggcga
    11161 cgctgaggac cgcgacggtg aggcccacgt ccgccagcgc acccaggccc gctcctcccc
    11221 gacgcccgtc ctcctcaccc ttgccttctt ctcttccctc tagagtcgtg tctggaaccc
    11281 ggcttttcca attggcctgc tgcatccgaa cagcgtgaac gtgagtgaat ttgcccgaag
    11341 cttgtctttg ctgcgcgggt ttggggacgg ctgcccgccc tcttttcctt cacatttcat
    11401 tgcatgggtt ccccaaccgc gttctctggt tcttctttgt gaccccagtc aatgtccttc
    11461 ctcccccggc gcccggtccc tcgccctggt ctgcggcgtt ctctctggaa tcttgccctg
    11521 ggccgtggac gcccaggaaa agagttgggt gcccccaggc agcctcgcgt tgtgggcgac
    11581 cgcgccatcc cggaagccgc aaggcgatct gagtcgcctc caggtctacc tgaaagctgt
    11641 cggccgggag ggcggggccc caaaaaggag cattcctgcg ggcttttgct cgacgatccc
    11701 ttgctgaggc tgttgtggcg agggtcctgc agaggtaccc gttctgcgcc cagacaggct
    11761 cgaagcacgc gtccctcttt cctcgcagtc cgcggccgcg gatccaccat ggtgagcaag
    11821 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
    11881 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
    11941 ctgaagctga tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
    12001 ctgggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
    12061 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
    12121 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
    12181 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
    12241 aactacaaca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
    12301 aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag
    12361 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
    12421 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
    12481 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagg cgcgccctag
    12541 tcggatatcc gtcgagcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
    12601 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
    12661 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
    12721 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
    12781 aagcttcttg aagacaaaca acg
//
