LOCUS       sequence-311136-       12205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Venus under SP gene promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Nagai Y, Nishitani N, Yasuda M, Ueda Y, Fukui Y, Andoh C, Shirakawa 
            H, Nakagawa T, Inoue KI, Nagayasu K, Kasparov S, Nakamura K, Kaneko 
            S
  TITLE     Identification of neuron-type specific promoters in monkey genome 
            and their functional validation in mice.
  JOURNAL   Biochem Biophys Res Commun. 2019 Oct 22;518(4):619-624. doi: 
            10.1016/j.bbrc.2019.08.101. Epub 2019 Aug 23.
  PUBMED    31451217
REFERENCE   2  (bases 1 to 12205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2019.08.101"; journalName: "Biochem Biophys Res 
            Commun"; date: "2019-10-22- 22"; volume: "518"; issue: "4"; pages: 
            "619-624"
COMMENT     Sequence Label: pTYF-super-mfSP-1.7k-Venus-WPRE
FEATURES             Location/Qualifiers
     source          1..12205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1570..1572,1573..1575,1576..2289)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1615..1636)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1876..1895)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2223..2244
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2318..2892,2893..2895,2896..2904)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2485..2502)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2712..2731
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2905..3345
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3370..3912
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     misc_feature    3996..4584
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4049..4069)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4467..4478)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4789..5013
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6187..6204)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6358..6946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6438..6457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7117..7908,7909..7977))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7740..7759
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7978..8082)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8150..8168
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8206..8228)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8328..8347
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9609..9629
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9672..9852
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11234..11467
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    11729..11846
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    11959..12053
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 aggcaatgag aaatatttga atgctaactg gatatttaag gtaaggaatt attttatgtt
       61 gttttaggtg ggaaaatggt atggttatgt ttttattttt ttaaatgctt accttttata
      121 cagtgatgct gaaatattta cagttgaaat gactaaatgt ctgggatttt ttttcaaaat
      181 aatttgagtg cagaggaaac tggaagggtt gtagatgaaa cctggaagtg gatgtacctc
      241 tctacttttg gagatgctga taattttcca tataaaattt ctgaaaacag aaaataaaat
      301 gctcacattg tgaggctaag gtcaaaagaa atcagaaatg catgagaatt tccaaatcca
      361 gggttaagaa caacatagag gaaaaaattt ctggtttaag ttgcaaaaat gtgtttcttt
      421 tatacaatat actaacaaat cataattaac tgaaaaatgt aacactttat atgtaaaggg
      481 gagaaaacta ctccaccaaa gatgccgcat tcatttagag atcaaagaaa tgtgtctgcc
      541 aggcaaccaa gggctcatgg aaagctgtgg tttgtgtaca aatgctattt gtctaatatt
      601 ttgtgcgtta atgactgtcc cattagcatc ttcactacac ttattttcat agaaaggaga
      661 aacatgattt atagagccct ttagtgacaa gggtgaggat cctatacatt atgttgctgg
      721 tttcctagtc ttcagcaaga aagtgtagga gagaagcaaa aaaagtcctg ttccaacccc
      781 tgctcctgga tgtgacaagg aagaggagtt acccagcttg aaacaaaaga aatcctaagt
      841 ctgacacaca atgtcatgtt taaattcccc tttctccaaa atgtaaaata aatgtgcttc
      901 catcttctaa aatactatgg gactaaacaa ccttttgtaa cgctaaggaa atgccagtat
      961 tctcattgat ttaggagagg gatgttctgg tcatagaaag atgctgtgtc tcagaaacac
     1021 ttaaatactc ttaagctaga aatagagggg aaaagaatgc ctccctgcat ctcccctcaa
     1081 ctgaagtcct cctttcttag cctgatttcc gatgaaatgt ctgaatgcct acagttattt
     1141 ggccatcctc aaaagtgcaa cttatcctga cgtctcaagg gacggaaaag ttaccgaagt
     1201 ccaaggaatg agtcactttg ctcaaatttg atgagtaatc tcaggtgtca tgaaacccag
     1261 tttcaaagga gaggggaggg ggcgtcagat ctgcagacgg aagcaggccg ctcccgattg
     1321 gatggcgaga cctcgatttt cctaaaattg cgtcatttag aacccaattg ggtccagatg
     1381 ttatgggcat cgacgagtta ccgtctcgga aactctcaat cacgcaagcg aaaggagagg
     1441 aggcggctaa ttaaatattg agcagaaagt cgcgtgggga gaatgtcacg tgggtttgga
     1501 ggctcgagga ggctgggata aataccgcaa ggcactcagc aggcgaaaga gcgcggccgc
     1561 ggatccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1621 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1681 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc
     1741 tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac
     1801 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1861 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1921 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1981 ctggggcaca agctggagta caactacaac agccacaacg tctatatcac cgccgacaag
     2041 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
     2101 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2161 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     2221 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2281 tacaagtaag gcgcgcccta gtcggatatc cgtcgagccc ctctccctcc ccccccccta
     2341 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     2401 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     2461 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     2521 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     2581 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     2641 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     2701 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     2761 taccccattg tatgggatct gatctggggc ctcggtacac atgctttaca tgtgtttagt
     2821 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     2881 cacgatgata atatggccac aaccatgaag ctactgtctt ctatcgaaca agcatgcgat
     2941 atttgccgac ttaaaaagct caagtgctcc aaagaaaaac cgaagtgcgc caagtgtctg
     3001 aagaacaact gggagtgtcg ctactctccc aaaaccaaaa ggtctccgct gactagggca
     3061 catctgacag aagtggaatc aaggctagaa agactggaac agctatttct actgattttt
     3121 cctcgagaag accttgacat gattttgaaa atggattctt tacaggatat aaaagcattg
     3181 ttaacaggat tatttgtaca agataatgtg aataaagatg ccgtcacaga tagattggct
     3241 tcagtggaga ctgatatgcc tctaacattg agacagcata gaataagtgc gacatcatca
     3301 tcggaagaga gtagtaacaa aggtcaaaga cagttgactg tatcgccgga attgggatcc
     3361 atggtgtttc cttctgggca gatctcaaac caggccctgg ccttagcacc gtcctctgcc
     3421 ccagtccttg cccagaccat ggtcccttcc tcagccatgg tacctctggc tcagccccca
     3481 gctcctgccc cagttctaac cccgggtcct ccccagtccc tgtctgcacc tgttccaaag
     3541 agcacccagg ctggggaagg cacgctgtcg gaagccctgc tgcacctgca gtttgatgct
     3601 gatgaagact tgggggcctt gcttggcaac agcacagacc caggagtgtt cacagacctg
     3661 gcatctgtgg acaactcaga gtttcagcag ctcctgaacc agggtgtgtc catgtctcac
     3721 tccacagctg agcccatgct gatggagtac cctgaagcta taactcgcct ggtgacaggg
     3781 tcccagaggc cccctgaccc agctcccaca cccctgggga cctcggggct tcccaatggt
     3841 ctctccggag atgaagactt ctcctccatt gcggacatgg acttctctgc tctgctgagt
     3901 cagatcagct ccagcggcca atctagactg cagctcgacc tcgagggggg gcccggtacc
     3961 aggtaagtta acatcgaggg atcaagctta tcgataatca acctctggat tacaaaattt
     4021 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4081 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4141 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4201 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4261 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4321 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4381 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     4441 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4501 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4561 tctccctttg ggccgcctcc ccgcatcgtc gactagggat aacagggtaa cggtaccttt
     4621 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     4681 actggaaggg ctaattcact cccagggtct ctctggttag accagatctg agcctgggag
     4741 ctctctggct aactagggaa cccactgctt aagcctcata aagctcgact gtgccttcta
     4801 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     4861 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     4921 attctattct ggggggtagg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     4981 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     5041 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     5101 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     5161 tcccttcctt tctcgccacg ttcgccggct gtgcgggaga acggagttct attatgactc
     5221 aaatcagtct ccccaagcat tcggggatca gagtttttaa ggataactta gtgtgtaggg
     5281 ggccagtgag ttggagatga aagcgtaggg agtcgaaggt gtccttttgc gccgagtcag
     5341 ttcctgggtg ggggccacaa gatcggatga gccagtttat caatccgggg gtgccagctg
     5401 atccatggag tgcagggtct gcaaaatatc tcaagcactg attgatctta ggttttacaa
     5461 tagtgatgtt accccaggaa caatttgggg aaggtcagaa tcttgtagcc tgtagctgca
     5521 tgactcctaa accataattt cttttttgtt tttttttttt tatttttgag acagggtctc
     5581 actctgtcac ctaggctgga gtgcagtggt gcaatcacag ctcactgcag cctcaacgtc
     5641 gtaagctcaa gcgatcctcc cacctcagcc tgcctggtag ctgagactac aagcgacgcc
     5701 ccagttaatt tttgtatttt tggtagaggc agcgttttgc cgtgtggccc tggctggtct
     5761 cgaactcctg ggctcaagtg atccagcctc agcctcccaa agtgctggga caaccggggc
     5821 cagtcactgc acctggccct aaaccataat ttctaatctt ttggctaatt tgttagtcct
     5881 acaaaggcag tctagtcccc aggcaaaaag ggggtttgtt tcgggaaagg gctgttactg
     5941 tctttgtttc aaactataaa ctaagttcct cctaaactta gttcggccta cacccaggaa
     6001 tgaacaagga gagcttggag gttagaagca cgatggaatt ggttaggtca gatctctttc
     6061 actgtctgag ttataatttt gcaatggtgg ttcaaagact gcccgcttct gacaccagtc
     6121 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6181 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6241 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6301 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6361 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6421 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6481 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6541 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6601 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6661 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6721 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6781 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6841 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6901 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6961 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7021 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7081 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7141 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7201 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7261 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7321 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7381 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7441 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7501 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7561 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7621 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7681 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     7741 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7801 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7861 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7921 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     7981 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8041 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8101 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     8161 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     8221 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     8281 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     8341 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     8401 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     8461 gcgggcctct tcgctattac gccaggggag gcagagattg cagtaagctg agatcgcagc
     8521 actgcactcc agcctgggcg acagagtaag actctgtctc aaaaataaaa taaataaatc
     8581 aatcagatat tccaatcttt tcctttattt atttatttat tttctatttt ggaaacacag
     8641 tccttcctta ttccagaatt acacatatat tctatttttc tttatatgct ccagtttttt
     8701 ttagaccttc acctgaaatg tgtgtataca aaatctaggc cagtccagca gagcctaaag
     8761 gtaaaaaata aaataataaa aaataaataa aatctagctc actccttcac atcaaaatgg
     8821 agatacagct gttagcatta aataccaaat aacccatctt gtcctcaata attttaagcg
     8881 cctctctcca ccacatctaa ctcctgtcaa aggcatgtgc cccttccggg cgctctgctg
     8941 tgctgccaac caactggcat gtggactctg cagggtccct aactgccaag ccccacagtg
     9001 tgccctgagg ctgccccttc cttctagcgg ctgcccccac tcggctttgc tttccctagt
     9061 ttcagttact tgcgttcagc caaggtctga aactaggtgc gcacagagcg gtaagactgc
     9121 gagagaaaga gaccagcttt acagggggtt tatcacagtg caccctgaca gtcgtcagcc
     9181 tcacaggggg tttatcacat tgcaccctga cagtcgtcag cctcacaggg ggtttatcac
     9241 agtgcaccct tacaatcatt ccatttgatt cacaattttt ttagtctcta ctgtgcctaa
     9301 cttgtaagtt aaatttgatc agaggtgtgt tcccagaggg gaaaacagta tatacagggt
     9361 tcagtactat cgcatttcag gcccgagagc tgcatccgga gtatctagat ggagttccgc
     9421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     9481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     9541 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
     9601 cccgttgacg caaatgggcg gtaggcgtgt actctagaag gtctatataa gcagagctcg
     9661 tttagtgaac cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     9721 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgctcaaa gtagtgtgtg
     9781 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9841 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagtaaagc cagaggagat
     9901 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     9961 gcagagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
    10021 cgtcggtatt aagcggggga gaattagata aatgggaaaa aattcggtta aggccagggg
    10081 gaaagaaaca atataaacta aaacatatag tatgggcaag cagggagcta gaacgattcg
    10141 cagttaatcc tggcctttta gagacatcag aaggctgtag acaaatactg ggacagctac
    10201 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca atagcagtcc
    10261 tctattgtgt gcatcaaagg atagatgtaa aagacaccaa ggaagcctta gataagatag
    10321 aggaagagca aaacaaaagt aagaaaaagg cacagcaagc agcagctgac acaggaaaca
    10381 acagccaggt cagccaaaat taccctatag tgcagaacct ccaggggcaa atggtacatc
    10441 aggccatatc acctagaact ttaaatgcat gggtaaaagt agtagaagag aaggctttca
    10501 gcccagaagt aatacccatg ttttcagcat tatcagaagg agccacccca caagatttaa
    10561 ataccatgct aaacacagtg gggggacatc aagcagccat gcaaatgtta aaagagacca
    10621 tcaatgagga agctgcagaa tgggatagat tgcatccagt gcatgcaggg cctattgcac
    10681 caggccagat gagagaacca aggggaagtg acatagcagg aactactagc aaattaagag
    10741 aacaatttgg aaataataaa acaataatct ttaagcaatc ctcaggaggg gacccagaaa
    10801 ttgtaacgca cagttttaat tgtggagggg aatttttcta ctgtaattca acacaactgt
    10861 ttaatagtac ttggtttaat agtacttgga gtactgaagg gtcaaataac actgaaggaa
    10921 gtgacacaat cacactccca tgcagaataa aacaatttat aaacatgtgg caggaagtag
    10981 gaaaagcaat gtatgcccct cccatcagtg gacaaattag atgttcatca aatattactg
    11041 ggctgctatt aacaagagat ggtggtaata acaacaatgg gtccgagatc ttcagacctg
    11101 gaggaggcga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
    11161 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
    11221 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
    11281 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctgat atagtgcagc
    11341 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
    11401 ggggcatcaa acagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
    11461 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
    11521 atgctagttg gagtaataaa tctctggaac agatttggaa taacatgacc tggatggagt
    11581 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
    11641 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggct tgcggccgga
    11701 attcacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
    11761 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
    11821 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
    11881 gggcggccta gggataacag ggtaatagat cttgctagct cttatcatgt ctggatccaa
    11941 gcttgcatgc ctgcaggtcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
    12001 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagac
    12061 tctagagggt cccgggatac gcgttttacg tcacccagac catggtactt tcttacagca
    12121 gcctgaacta acaaaaccaa ttataatatg tggacttcat tataattctg atttcaacta
    12181 gtaaactgcg tggggaggaa ttagg
//
