LOCUS       sequence-311138-       12409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Venus under PV gene promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12409)
  AUTHORS   Nagai Y, Nishitani N, Yasuda M, Ueda Y, Fukui Y, Andoh C, Shirakawa 
            H, Nakagawa T, Inoue KI, Nagayasu K, Kasparov S, Nakamura K, Kaneko 
            S
  TITLE     Identification of neuron-type specific promoters in monkey genome 
            and their functional validation in mice.
  JOURNAL   Biochem Biophys Res Commun. 2019 Oct 22;518(4):619-624. doi: 
            10.1016/j.bbrc.2019.08.101. Epub 2019 Aug 23.
  PUBMED    31451217
REFERENCE   2  (bases 1 to 12409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2019.08.101"; journalName: "Biochem Biophys Res 
            Commun"; date: "2019-10-22- 22"; volume: "518"; issue: "4"; pages: 
            "619-624"
COMMENT     Sequence Label: pTYF-super-mfPV-1.8k-Venus-WPRE
FEATURES             Location/Qualifiers
     source          1..12409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     135..154
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             328..768
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             793..1335
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     misc_feature    1419..2007
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1472..1492)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1890..1901)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2212..2436
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2569..2588)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(3610..3627)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3781..4369)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3861..3880)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4540..5331,5332..5400))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5163..5182
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5401..5505)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5573..5591
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5629..5651)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5751..5770
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7032..7052
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7095..7275
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8657..8890
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9152..9269
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    9382..9476
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             join(11402..11404,11405..11407,11408..12121)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(11447..11468)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11708..11727)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12055..12076
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(12150..12409,1..315,316..318,319..327)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(12317..12334)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
       61 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
      121 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
      181 aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt acatgtgttt
      241 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
      301 aaacacgatg ataatatggc cacaaccatg aagctactgt cttctatcga acaagcatgc
      361 gatatttgcc gacttaaaaa gctcaagtgc tccaaagaaa aaccgaagtg cgccaagtgt
      421 ctgaagaaca actgggagtg tcgctactct cccaaaacca aaaggtctcc gctgactagg
      481 gcacatctga cagaagtgga atcaaggcta gaaagactgg aacagctatt tctactgatt
      541 tttcctcgag aagaccttga catgattttg aaaatggatt ctttacagga tataaaagca
      601 ttgttaacag gattatttgt acaagataat gtgaataaag atgccgtcac agatagattg
      661 gcttcagtgg agactgatat gcctctaaca ttgagacagc atagaataag tgcgacatca
      721 tcatcggaag agagtagtaa caaaggtcaa agacagttga ctgtatcgcc ggaattggga
      781 tccatggtgt ttccttctgg gcagatctca aaccaggccc tggccttagc accgtcctct
      841 gccccagtcc ttgcccagac catggtccct tcctcagcca tggtacctct ggctcagccc
      901 ccagctcctg ccccagttct aaccccgggt cctccccagt ccctgtctgc acctgttcca
      961 aagagcaccc aggctgggga aggcacgctg tcggaagccc tgctgcacct gcagtttgat
     1021 gctgatgaag acttgggggc cttgcttggc aacagcacag acccaggagt gttcacagac
     1081 ctggcatctg tggacaactc agagtttcag cagctcctga accagggtgt gtccatgtct
     1141 cactccacag ctgagcccat gctgatggag taccctgaag ctataactcg cctggtgaca
     1201 gggtcccaga ggccccctga cccagctccc acacccctgg ggacctcggg gcttcccaat
     1261 ggtctctccg gagatgaaga cttctcctcc attgcggaca tggacttctc tgctctgctg
     1321 agtcagatca gctccagcgg ccaatctaga ctgcagctcg acctcgaggg ggggcccggt
     1381 accaggtaag ttaacatcga gggatcaagc ttatcgataa tcaacctctg gattacaaaa
     1441 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     1501 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     1561 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     1621 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     1681 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     1741 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     1801 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     1861 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     1921 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     1981 ggatctccct ttgggccgcc tccccgcatc gtcgactagg gataacaggg taacggtacc
     2041 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     2101 gggactggaa gggctaattc actcccaggg tctctctggt tagaccagat ctgagcctgg
     2161 gagctctctg gctaactagg gaacccactg cttaagcctc ataaagctcg actgtgcctt
     2221 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     2281 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     2341 gtcattctat tctggggggt agggtggggc aggacagcaa gggggaggat tgggaagaca
     2401 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     2461 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     2521 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     2581 tcttcccttc ctttctcgcc acgttcgccg gctgtgcggg agaacggagt tctattatga
     2641 ctcaaatcag tctccccaag cattcgggga tcagagtttt taaggataac ttagtgtgta
     2701 gggggccagt gagttggaga tgaaagcgta gggagtcgaa ggtgtccttt tgcgccgagt
     2761 cagttcctgg gtgggggcca caagatcgga tgagccagtt tatcaatccg ggggtgccag
     2821 ctgatccatg gagtgcaggg tctgcaaaat atctcaagca ctgattgatc ttaggtttta
     2881 caatagtgat gttaccccag gaacaatttg gggaaggtca gaatcttgta gcctgtagct
     2941 gcatgactcc taaaccataa tttctttttt gttttttttt ttttattttt gagacagggt
     3001 ctcactctgt cacctaggct ggagtgcagt ggtgcaatca cagctcactg cagcctcaac
     3061 gtcgtaagct caagcgatcc tcccacctca gcctgcctgg tagctgagac tacaagcgac
     3121 gccccagtta atttttgtat ttttggtaga ggcagcgttt tgccgtgtgg ccctggctgg
     3181 tctcgaactc ctgggctcaa gtgatccagc ctcagcctcc caaagtgctg ggacaaccgg
     3241 ggccagtcac tgcacctggc cctaaaccat aatttctaat cttttggcta atttgttagt
     3301 cctacaaagg cagtctagtc cccaggcaaa aagggggttt gtttcgggaa agggctgtta
     3361 ctgtctttgt ttcaaactat aaactaagtt cctcctaaac ttagttcggc ctacacccag
     3421 gaatgaacaa ggagagcttg gaggttagaa gcacgatgga attggttagg tcagatctct
     3481 ttcactgtct gagttataat tttgcaatgg tggttcaaag actgcccgct tctgacacca
     3541 gtcgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     3601 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3661 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3721 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3781 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3841 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3901 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3961 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4021 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4081 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4141 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4201 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4261 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4321 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4381 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4441 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4501 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4561 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4621 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4681 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4741 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4801 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4861 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4921 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4981 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5041 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5101 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5161 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5221 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5281 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5341 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5401 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5461 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5521 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     5581 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     5641 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     5701 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     5761 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     5821 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     5881 ggtgcgggcc tcttcgctat tacgccaggg gaggcagaga ttgcagtaag ctgagatcgc
     5941 agcactgcac tccagcctgg gcgacagagt aagactctgt ctcaaaaata aaataaataa
     6001 atcaatcaga tattccaatc ttttccttta tttatttatt tattttctat tttggaaaca
     6061 cagtccttcc ttattccaga attacacata tattctattt ttctttatat gctccagttt
     6121 tttttagacc ttcacctgaa atgtgtgtat acaaaatcta ggccagtcca gcagagccta
     6181 aaggtaaaaa ataaaataat aaaaaataaa taaaatctag ctcactcctt cacatcaaaa
     6241 tggagataca gctgttagca ttaaatacca aataacccat cttgtcctca ataattttaa
     6301 gcgcctctct ccaccacatc taactcctgt caaaggcatg tgccccttcc gggcgctctg
     6361 ctgtgctgcc aaccaactgg catgtggact ctgcagggtc cctaactgcc aagccccaca
     6421 gtgtgccctg aggctgcccc ttccttctag cggctgcccc cactcggctt tgctttccct
     6481 agtttcagtt acttgcgttc agccaaggtc tgaaactagg tgcgcacaga gcggtaagac
     6541 tgcgagagaa agagaccagc tttacagggg gtttatcaca gtgcaccctg acagtcgtca
     6601 gcctcacagg gggtttatca cattgcaccc tgacagtcgt cagcctcaca gggggtttat
     6661 cacagtgcac ccttacaatc attccatttg attcacaatt tttttagtct ctactgtgcc
     6721 taacttgtaa gttaaatttg atcagaggtg tgttcccaga ggggaaaaca gtatatacag
     6781 ggttcagtac tatcgcattt caggcccgag agctgcatcc ggagtatcta gatggagttc
     6841 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     6901 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     6961 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
     7021 cgccccgttg acgcaaatgg gcggtaggcg tgtactctag aaggtctata taagcagagc
     7081 tcgtttagtg aaccgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7141 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctc aaagtagtgt
     7201 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7261 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaagtaa agccagagga
     7321 gatctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     7381 actgcagagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     7441 gagcgtcggt attaagcggg ggagaattag ataaatggga aaaaattcgg ttaaggccag
     7501 ggggaaagaa acaatataaa ctaaaacata tagtatgggc aagcagggag ctagaacgat
     7561 tcgcagttaa tcctggcctt ttagagacat cagaaggctg tagacaaata ctgggacagc
     7621 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acaatagcag
     7681 tcctctattg tgtgcatcaa aggatagatg taaaagacac caaggaagcc ttagataaga
     7741 tagaggaaga gcaaaacaaa agtaagaaaa aggcacagca agcagcagct gacacaggaa
     7801 acaacagcca ggtcagccaa aattacccta tagtgcagaa cctccagggg caaatggtac
     7861 atcaggccat atcacctaga actttaaatg catgggtaaa agtagtagaa gagaaggctt
     7921 tcagcccaga agtaataccc atgttttcag cattatcaga aggagccacc ccacaagatt
     7981 taaataccat gctaaacaca gtggggggac atcaagcagc catgcaaatg ttaaaagaga
     8041 ccatcaatga ggaagctgca gaatgggata gattgcatcc agtgcatgca gggcctattg
     8101 caccaggcca gatgagagaa ccaaggggaa gtgacatagc aggaactact agcaaattaa
     8161 gagaacaatt tggaaataat aaaacaataa tctttaagca atcctcagga ggggacccag
     8221 aaattgtaac gcacagtttt aattgtggag gggaattttt ctactgtaat tcaacacaac
     8281 tgtttaatag tacttggttt aatagtactt ggagtactga agggtcaaat aacactgaag
     8341 gaagtgacac aatcacactc ccatgcagaa taaaacaatt tataaacatg tggcaggaag
     8401 taggaaaagc aatgtatgcc cctcccatca gtggacaaat tagatgttca tcaaatatta
     8461 ctgggctgct attaacaaga gatggtggta ataacaacaa tgggtccgag atcttcagac
     8521 ctggaggagg cgatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     8581 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     8641 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     8701 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct gatatagtgc
     8761 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     8821 tctggggcat caaacagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     8881 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     8941 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaataacatg acctggatgg
     9001 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     9061 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcttgcggcc
     9121 ggaattcaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     9181 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     9241 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     9301 tttgggcggc ctagggataa cagggtaata gatcttgcta gctcttatca tgtctggatc
     9361 caagcttgca tgcctgcagg tcggagtact gtcctccgag cggagtactg tcctccgagc
     9421 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     9481 gactctagag ggtcccggga tacgcgtctg agctgagatg tatggcacct gatagagctg
     9541 ggtcccaggc accagggagg atcacccaga gagtcactga actccacatg agtgagccca
     9601 ggggtgtgct ctgctctgct attgctttgc catgtgtcct cagaccactc acggattctc
     9661 tgcgcctcca tttctccact ggtatagctg aagggtcttt gagctgggga aatccattct
     9721 ccaactagga gtgagctgag cctgggagtt gaaatgatag cgtaggagcc tggcatgtct
     9781 gagtcagcag cccagctggg gacaaggtgc agaggtgacg gcagtagcag caggggccag
     9841 ggcagctgag ggatcctggg aagtcctgga tcagagaggc atcaggtcag gagccgcagt
     9901 ctgggtgcct tggaagccag ctggccggct gtggcctgaa ggagctgact cacagggagg
     9961 gggcagctgg agccagcaac ggtatcctaa ggtttccaag aagggcaccc atccacggct
    10021 gagctgcagg agacagtgtt gaccatgatg tcagggaact tgagctactg ggcagctgga
    10081 cagctgctct ccctgggccc caccaccgag ggccaccaca gccccaggcc ctgcctacct
    10141 gcccatacct taagttgcag tccctaggtc ctactacatg ggaccactga catcaacaca
    10201 tcatcgagag acagaagccc agagaaggtc acaacccaag gtcacacagg gaattagcac
    10261 agcccaggat ctagaaccca ggcctcctga ctccctgtcc agtgctcaca cactctagaa
    10321 gcagctggtt gcagcttgaa accttgggaa ccgtggggaa gaacacaact gatttctggc
    10381 tgcatgaaga tagacactgt gacatttttc cagaggtgaa gttcagagcc cccacgcctg
    10441 cttagattgt ctagaaagtg tggtctgaag gtgctaccct tcttccaaac atatgcgggg
    10501 ctttctggga gggaccttag caatttcaag cagcctcccc caccctactc tgcccaaaaa
    10561 ggtttatgga ggcaggggga gtctggtccc atgatgccag gacccaggag accaggaaag
    10621 caagtagtta gtagagatct gagaagtgtt ctttgaaaaa tcaagccaac aaaaaaatga
    10681 aaataaaaca aatcaggttt tagaatgtgc atccctggcc agatgtggga tggggcaccc
    10741 gaagtgggcc ccagcagcta ctgcttgtcc ctgtccctgt ccctgccacc tcctccctac
    10801 gtccgtgggt caggtttcac gacctcagcg ctcagatttt gcagcataaa tttgcgtcca
    10861 ggaaagacca gagcagaggc tgaagtagga gtggagagag aaagagagca ggtcctaggg
    10921 ggtcctgggg tgcaggctgg ccgctgaggg ttcaggatct tccgccacgc ggcaggacat
    10981 ccttggaggt tcccagatct gagaccccgc acctcctgcg tgtccttgac agcaggtgga
    11041 ctttccagac ctggctggtg actccagtgc cccctaaatc ccagaggtgg cccccttccc
    11101 cttgtgccct ggccgcagcc cctgggatcc cgcggacccc cggcctggcg gtgcaggagc
    11161 ggtagcaggt ccgcgcgccc tcttgcccgc ggccggcgct gctcagccgc gcgactggac
    11221 tgagccgctc ccgctgcgct cagagtccgg gcccctggcg ccatgccagg cccggcgggc
    11281 gtgcgcccgg cgcgcagcac tgcagcgctg gtcatatgag cagaaatgat gagaaaagca
    11341 ctttttaatc ttttcgcact tgctctgccc gctcaaacag ttgcgcggcc gcggatccac
    11401 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
    11461 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
    11521 cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc cctggcccac
    11581 cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa
    11641 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
    11701 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
    11761 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
    11821 caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca agcagaagaa
    11881 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc
    11941 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
    12001 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
    12061 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
    12121 aggcgcgccc tagtcggata tccgtcgagc ccctctccct cccccccccc taacgttact
    12181 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
    12241 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
    12301 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
    12361 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccc
//
