LOCUS       sequence-311139-       11010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Venus under CCK gene promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11010)
  AUTHORS   Nagai Y, Nishitani N, Yasuda M, Ueda Y, Fukui Y, Andoh C, Shirakawa 
            H, Nakagawa T, Inoue KI, Nagayasu K, Kasparov S, Nakamura K, Kaneko 
            S
  TITLE     Identification of neuron-type specific promoters in monkey genome 
            and their functional validation in mice.
  JOURNAL   Biochem Biophys Res Commun. 2019 Oct 22;518(4):619-624. doi: 
            10.1016/j.bbrc.2019.08.101. Epub 2019 Aug 23.
  PUBMED    31451217
REFERENCE   2  (bases 1 to 11010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2019.08.101"; journalName: "Biochem Biophys Res 
            Commun"; date: "2019-10-22- 22"; volume: "518"; issue: "4"; pages: 
            "619-624"
COMMENT     Sequence Label: pTYF-super-mfCCK-0.5k-Venus-WPRE
FEATURES             Location/Qualifiers
     source          1..11010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    111..205
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             join(732..734,735..737,738..1451)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(777..798)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1038..1057)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1385..1406
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(1480..2054,2055..2057,2058..2066)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1647..1664)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1874..1893
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2067..2507
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             2532..3074
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     misc_feature    3158..3746
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3211..3231)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3629..3640)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3951..4175
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4308..4327)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(5349..5366)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5520..6108)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5600..5619)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6279..7070,7071..7139))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6902..6921
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7140..7244)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7312..7330
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7368..7390)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7490..7509
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8771..8791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8834..9014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10396..10629
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10891..11008
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggtttattac agggacagca gagatccact ttgggcggcc tagggataac agggtaatag
       61 atcttgctag ctcttatcat gtctggatcc aagcttgcat gcctgcaggt cggagtactg
      121 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
      181 tccgagcgga gtactgtcct ccgagcggag actctagagg gtcccgggat acgcgttgcc
      241 atttcccacc tacactttga gaaaacttta taaatacggc tttatttatt gctcctacat
      301 tttaaactgt gcttctcttt ttcgtcattt actgtatttc catattgaaa gcttgttagc
      361 tatctggaaa ggtgctttcc ctagggttct catttctccc acggagtctt ccctttagga
      421 tatttttttc acactatttt taatctcgtg gggttttatt tttcttcttt cagttagaag
      481 agaggcactc tgcaaacact tcctcaaact ttagggaagc agaaaacatt ccttctgctc
      541 aacacggcaa aaaacagggt ctctgtctgc ccctcggcac tgggtgtgga gttcccaaag
      601 accagccgtg tgcgcccggg ggcgcgcgcc atggcactcg gcttgagccc tcgtgcttgt
      661 ggtggcctgg ggagtctgga gcacctgtgt ttgtcctaag ccggtttctg ggtggcggcc
      721 gcggatccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      781 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      841 atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc
      901 cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg
      961 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1021 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1081 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1141 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca
     1201 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     1261 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1321 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg
     1381 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     1441 tgtacaagta aggcgcgccc tagtcggata tccgtcgagc ccctctccct cccccccccc
     1501 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     1561 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     1621 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     1681 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     1741 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     1801 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     1861 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     1921 ggtaccccat tgtatgggat ctgatctggg gcctcggtac acatgcttta catgtgttta
     1981 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     2041 aacacgatga taatatggcc acaaccatga agctactgtc ttctatcgaa caagcatgcg
     2101 atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa accgaagtgc gccaagtgtc
     2161 tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa aaggtctccg ctgactaggg
     2221 cacatctgac agaagtggaa tcaaggctag aaagactgga acagctattt ctactgattt
     2281 ttcctcgaga agaccttgac atgattttga aaatggattc tttacaggat ataaaagcat
     2341 tgttaacagg attatttgta caagataatg tgaataaaga tgccgtcaca gatagattgg
     2401 cttcagtgga gactgatatg cctctaacat tgagacagca tagaataagt gcgacatcat
     2461 catcggaaga gagtagtaac aaaggtcaaa gacagttgac tgtatcgccg gaattgggat
     2521 ccatggtgtt tccttctggg cagatctcaa accaggccct ggccttagca ccgtcctctg
     2581 ccccagtcct tgcccagacc atggtccctt cctcagccat ggtacctctg gctcagcccc
     2641 cagctcctgc cccagttcta accccgggtc ctccccagtc cctgtctgca cctgttccaa
     2701 agagcaccca ggctggggaa ggcacgctgt cggaagccct gctgcacctg cagtttgatg
     2761 ctgatgaaga cttgggggcc ttgcttggca acagcacaga cccaggagtg ttcacagacc
     2821 tggcatctgt ggacaactca gagtttcagc agctcctgaa ccagggtgtg tccatgtctc
     2881 actccacagc tgagcccatg ctgatggagt accctgaagc tataactcgc ctggtgacag
     2941 ggtcccagag gccccctgac ccagctccca cacccctggg gacctcgggg cttcccaatg
     3001 gtctctccgg agatgaagac ttctcctcca ttgcggacat ggacttctct gctctgctga
     3061 gtcagatcag ctccagcggc caatctagac tgcagctcga cctcgagggg gggcccggta
     3121 ccaggtaagt taacatcgag ggatcaagct tatcgataat caacctctgg attacaaaat
     3181 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     3241 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     3301 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     3361 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     3421 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     3481 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     3541 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     3601 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     3661 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     3721 gatctccctt tgggccgcct ccccgcatcg tcgactaggg ataacagggt aacggtacct
     3781 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     3841 ggactggaag ggctaattca ctcccagggt ctctctggtt agaccagatc tgagcctggg
     3901 agctctctgg ctaactaggg aacccactgc ttaagcctca taaagctcga ctgtgccttc
     3961 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     4021 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     4081 tcattctatt ctggggggta gggtggggca ggacagcaag ggggaggatt gggaagacaa
     4141 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     4201 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4261 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4321 cttcccttcc tttctcgcca cgttcgccgg ctgtgcggga gaacggagtt ctattatgac
     4381 tcaaatcagt ctccccaagc attcggggat cagagttttt aaggataact tagtgtgtag
     4441 ggggccagtg agttggagat gaaagcgtag ggagtcgaag gtgtcctttt gcgccgagtc
     4501 agttcctggg tgggggccac aagatcggat gagccagttt atcaatccgg gggtgccagc
     4561 tgatccatgg agtgcagggt ctgcaaaata tctcaagcac tgattgatct taggttttac
     4621 aatagtgatg ttaccccagg aacaatttgg ggaaggtcag aatcttgtag cctgtagctg
     4681 catgactcct aaaccataat ttcttttttg tttttttttt tttatttttg agacagggtc
     4741 tcactctgtc acctaggctg gagtgcagtg gtgcaatcac agctcactgc agcctcaacg
     4801 tcgtaagctc aagcgatcct cccacctcag cctgcctggt agctgagact acaagcgacg
     4861 ccccagttaa tttttgtatt tttggtagag gcagcgtttt gccgtgtggc cctggctggt
     4921 ctcgaactcc tgggctcaag tgatccagcc tcagcctccc aaagtgctgg gacaaccggg
     4981 gccagtcact gcacctggcc ctaaaccata atttctaatc ttttggctaa tttgttagtc
     5041 ctacaaaggc agtctagtcc ccaggcaaaa agggggtttg tttcgggaaa gggctgttac
     5101 tgtctttgtt tcaaactata aactaagttc ctcctaaact tagttcggcc tacacccagg
     5161 aatgaacaag gagagcttgg aggttagaag cacgatggaa ttggttaggt cagatctctt
     5221 tcactgtctg agttataatt ttgcaatggt ggttcaaaga ctgcccgctt ctgacaccag
     5281 tcgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5341 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5401 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5461 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5521 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5581 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5641 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5701 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5761 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5821 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5881 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5941 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6001 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6061 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6121 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6181 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6241 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6301 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6361 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6421 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6481 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6541 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6601 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6661 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6721 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6781 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6841 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6901 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     6961 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7021 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7081 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7141 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7201 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7261 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     7321 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     7381 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     7441 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     7501 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     7561 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     7621 gtgcgggcct cttcgctatt acgccagggg aggcagagat tgcagtaagc tgagatcgca
     7681 gcactgcact ccagcctggg cgacagagta agactctgtc tcaaaaataa aataaataaa
     7741 tcaatcagat attccaatct tttcctttat ttatttattt attttctatt ttggaaacac
     7801 agtccttcct tattccagaa ttacacatat attctatttt tctttatatg ctccagtttt
     7861 ttttagacct tcacctgaaa tgtgtgtata caaaatctag gccagtccag cagagcctaa
     7921 aggtaaaaaa taaaataata aaaaataaat aaaatctagc tcactccttc acatcaaaat
     7981 ggagatacag ctgttagcat taaataccaa ataacccatc ttgtcctcaa taattttaag
     8041 cgcctctctc caccacatct aactcctgtc aaaggcatgt gccccttccg ggcgctctgc
     8101 tgtgctgcca accaactggc atgtggactc tgcagggtcc ctaactgcca agccccacag
     8161 tgtgccctga ggctgcccct tccttctagc ggctgccccc actcggcttt gctttcccta
     8221 gtttcagtta cttgcgttca gccaaggtct gaaactaggt gcgcacagag cggtaagact
     8281 gcgagagaaa gagaccagct ttacaggggg tttatcacag tgcaccctga cagtcgtcag
     8341 cctcacaggg ggtttatcac attgcaccct gacagtcgtc agcctcacag ggggtttatc
     8401 acagtgcacc cttacaatca ttccatttga ttcacaattt ttttagtctc tactgtgcct
     8461 aacttgtaag ttaaatttga tcagaggtgt gttcccagag gggaaaacag tatatacagg
     8521 gttcagtact atcgcatttc aggcccgaga gctgcatccg gagtatctag atggagttcc
     8581 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     8641 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     8701 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc
     8761 gccccgttga cgcaaatggg cggtaggcgt gtactctaga aggtctatat aagcagagct
     8821 cgtttagtga accgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8881 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgctca aagtagtgtg
     8941 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9001 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagtaaa gccagaggag
     9061 atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     9121 ctgcagagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     9181 agcgtcggta ttaagcgggg gagaattaga taaatgggaa aaaattcggt taaggccagg
     9241 gggaaagaaa caatataaac taaaacatat agtatgggca agcagggagc tagaacgatt
     9301 cgcagttaat cctggccttt tagagacatc agaaggctgt agacaaatac tgggacagct
     9361 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata caatagcagt
     9421 cctctattgt gtgcatcaaa ggatagatgt aaaagacacc aaggaagcct tagataagat
     9481 agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa gcagcagctg acacaggaaa
     9541 caacagccag gtcagccaaa attaccctat agtgcagaac ctccaggggc aaatggtaca
     9601 tcaggccata tcacctagaa ctttaaatgc atgggtaaaa gtagtagaag agaaggcttt
     9661 cagcccagaa gtaataccca tgttttcagc attatcagaa ggagccaccc cacaagattt
     9721 aaataccatg ctaaacacag tggggggaca tcaagcagcc atgcaaatgt taaaagagac
     9781 catcaatgag gaagctgcag aatgggatag attgcatcca gtgcatgcag ggcctattgc
     9841 accaggccag atgagagaac caaggggaag tgacatagca ggaactacta gcaaattaag
     9901 agaacaattt ggaaataata aaacaataat ctttaagcaa tcctcaggag gggacccaga
     9961 aattgtaacg cacagtttta attgtggagg ggaatttttc tactgtaatt caacacaact
    10021 gtttaatagt acttggttta atagtacttg gagtactgaa gggtcaaata acactgaagg
    10081 aagtgacaca atcacactcc catgcagaat aaaacaattt ataaacatgt ggcaggaagt
    10141 aggaaaagca atgtatgccc ctcccatcag tggacaaatt agatgttcat caaatattac
    10201 tgggctgcta ttaacaagag atggtggtaa taacaacaat gggtccgaga tcttcagacc
    10261 tggaggaggc gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
    10321 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
    10381 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
    10441 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg atatagtgca
    10501 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
    10561 ctggggcatc aaacagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
    10621 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
    10681 gaatgctagt tggagtaata aatctctgga acagatttgg aataacatga cctggatgga
    10741 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
    10801 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg cttgcggccg
    10861 gaattcacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
    10921 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
    10981 aaaacaaatt acaaaaattc aaaattttcg
//
