LOCUS       sequence-311144-       11427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Venus under PENK gene promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Nagai Y, Nishitani N, Yasuda M, Ueda Y, Fukui Y, Andoh C, Shirakawa 
            H, Nakagawa T, Inoue KI, Nagayasu K, Kasparov S, Nakamura K, Kaneko 
            S
  TITLE     Identification of neuron-type specific promoters in monkey genome 
            and their functional validation in mice.
  JOURNAL   Biochem Biophys Res Commun. 2019 Oct 22;518(4):619-624. doi: 
            10.1016/j.bbrc.2019.08.101. Epub 2019 Aug 23.
  PUBMED    31451217
REFERENCE   2  (bases 1 to 11427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2019.08.101"; journalName: "Biochem Biophys Res 
            Commun"; date: "2019-10-22- 22"; volume: "518"; issue: "4"; pages: 
            "619-624"
COMMENT     Sequence Label: pTYF-super-mfPENK-0.9k-Venus-WPRE
FEATURES             Location/Qualifiers
     source          1..11427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    75..169
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             join(1074..1076,1077..1079,1080..1793)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1119..1140)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1380..1399)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1727..1748
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(1822..2396,2397..2399,2400..2408)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1989..2006)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2216..2235
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2409..2849
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             2874..3416
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     misc_feature    3500..4088
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3553..3573)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3971..3982)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4293..4517
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4650..4669)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(5691..5708)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5862..6450)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5942..5961)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6621..7412,7413..7481))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7244..7263
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7482..7586)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7654..7672
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7710..7732)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9152..9172
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9215..9395
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10777..11010
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    11272..11389
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctaggga taacagggta atagatcttg ctagctctta tcatgtctgg atccaagctt
       61 gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
      121 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagactcta
      181 gagggtcccg ggatatttgt cacctggccg tgtcgggagc caccgagcgc cccccgtggc
      241 cgtcaccctc ggtgggggaa gcggcgcgcg ggtactgggg gaccgacccc tcccgcgaag
      301 gcgtcggcgc ggggctggcg tagggcctgc gtcagctgca gcccgccggc gattggggag
      361 cgcgcgcctc cttcggtttg gggctaatta taaagtggct ccagccgccg ttaagccccg
      421 ggacggcgag gcaggcgctc agagccccgc ggcgacgctg aggaccgcga cggtgaggcc
      481 cacgtccgcc agcgcaccca ggcccgctcc tccccgacgc ccgtcctcct cacccttgcc
      541 ttcttctctt ccctctagag tcgtgtctgg aacccggctt ttccaattgg cctgctgcat
      601 ccgaacagcg tgaacgtgag tgaatttgcc cgaagcttgt ctttgctgcg cgggtttggg
      661 gacggctgcc cgccctcttt tccttcacat ttcattgcat gggttcccca accgcgttct
      721 ctggttcttc tttgtgaccc cagtcaatgt ccttcctccc ccggcgcccg gtccctcgcc
      781 ctggtctgcg gcgttctctc tggaatcttg ccctgggccg tggacgccca ggaaaagagt
      841 tgggtgcccc aggcagcctc gcgttggggg cgaccgcgcc atcccgggag ccgcaaggcg
      901 atctgagtcg cctccaggtc tacctgaaag ctgtcggccg ggagggcggg gccccaaaaa
      961 ggagcattcc tgcgggcttt tgctcgacga tcccttgctg aggctgttgt ggcgagggtc
     1021 ctgcagaggt acccgttctg cgcccagaca ggctcgaagc acgcggatcc accatggtga
     1081 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1141 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1201 tgaccctgaa gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1261 ccaccctggg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     1321 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1381 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1441 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1501 agtacaacta caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     1561 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact
     1621 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1681 gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1741 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaggcgcgc
     1801 cctagtcgga tatccgtcga gcccctctcc ctcccccccc cctaacgtta ctggccgaag
     1861 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     1921 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     1981 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     2041 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     2101 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     2161 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     2221 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     2281 atctgatctg gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     2341 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     2401 ccacaaccat gaagctactg tcttctatcg aacaagcatg cgatatttgc cgacttaaaa
     2461 agctcaagtg ctccaaagaa aaaccgaagt gcgccaagtg tctgaagaac aactgggagt
     2521 gtcgctactc tcccaaaacc aaaaggtctc cgctgactag ggcacatctg acagaagtgg
     2581 aatcaaggct agaaagactg gaacagctat ttctactgat ttttcctcga gaagaccttg
     2641 acatgatttt gaaaatggat tctttacagg atataaaagc attgttaaca ggattatttg
     2701 tacaagataa tgtgaataaa gatgccgtca cagatagatt ggcttcagtg gagactgata
     2761 tgcctctaac attgagacag catagaataa gtgcgacatc atcatcggaa gagagtagta
     2821 acaaaggtca aagacagttg actgtatcgc cggaattggg atccatggtg tttccttctg
     2881 ggcagatctc aaaccaggcc ctggccttag caccgtcctc tgccccagtc cttgcccaga
     2941 ccatggtccc ttcctcagcc atggtacctc tggctcagcc cccagctcct gccccagttc
     3001 taaccccggg tcctccccag tccctgtctg cacctgttcc aaagagcacc caggctgggg
     3061 aaggcacgct gtcggaagcc ctgctgcacc tgcagtttga tgctgatgaa gacttggggg
     3121 ccttgcttgg caacagcaca gacccaggag tgttcacaga cctggcatct gtggacaact
     3181 cagagtttca gcagctcctg aaccagggtg tgtccatgtc tcactccaca gctgagccca
     3241 tgctgatgga gtaccctgaa gctataactc gcctggtgac agggtcccag aggccccctg
     3301 acccagctcc cacacccctg gggacctcgg ggcttcccaa tggtctctcc ggagatgaag
     3361 acttctcctc cattgcggac atggacttct ctgctctgct gagtcagatc agctccagcg
     3421 gccaatctag actgcagctc gacctcgagg gggggcccgg taccaggtaa gttaacatcg
     3481 agggatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3541 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3601 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3661 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3721 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3781 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3841 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     3901 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     3961 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4021 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4081 ctccccgcat cgtcgactag ggataacagg gtaacggtac ctttaagacc aatgacttac
     4141 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     4201 cactcccagg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4261 ggaacccact gcttaagcct cataaagctc gactgtgcct tctagttgcc agccatctgt
     4321 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4381 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4441 tagggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     4501 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     4561 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4621 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4681 cacgttcgcc ggctgtgcgg gagaacggag ttctattatg actcaaatca gtctccccaa
     4741 gcattcgggg atcagagttt ttaaggataa cttagtgtgt agggggccag tgagttggag
     4801 atgaaagcgt agggagtcga aggtgtcctt ttgcgccgag tcagttcctg ggtgggggcc
     4861 acaagatcgg atgagccagt ttatcaatcc gggggtgcca gctgatccat ggagtgcagg
     4921 gtctgcaaaa tatctcaagc actgattgat cttaggtttt acaatagtga tgttacccca
     4981 ggaacaattt ggggaaggtc agaatcttgt agcctgtagc tgcatgactc ctaaaccata
     5041 atttcttttt tgtttttttt tttttatttt tgagacaggg tctcactctg tcacctaggc
     5101 tggagtgcag tggtgcaatc acagctcact gcagcctcaa cgtcgtaagc tcaagcgatc
     5161 ctcccacctc agcctgcctg gtagctgaga ctacaagcga cgccccagtt aatttttgta
     5221 tttttggtag aggcagcgtt ttgccgtgtg gccctggctg gtctcgaact cctgggctca
     5281 agtgatccag cctcagcctc ccaaagtgct gggacaaccg gggccagtca ctgcacctgg
     5341 ccctaaacca taatttctaa tcttttggct aatttgttag tcctacaaag gcagtctagt
     5401 ccccaggcaa aaagggggtt tgtttcggga aagggctgtt actgtctttg tttcaaacta
     5461 taaactaagt tcctcctaaa cttagttcgg cctacaccca ggaatgaaca aggagagctt
     5521 ggaggttaga agcacgatgg aattggttag gtcagatctc tttcactgtc tgagttataa
     5581 ttttgcaatg gtggttcaaa gactgcccgc ttctgacacc agtcgctgca ttaatgaatc
     5641 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5701 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5761 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5821 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5881 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5941 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6001 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6061 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6121 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6181 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6241 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6301 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6361 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6421 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6481 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6541 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6601 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6661 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6721 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6781 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6841 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6901 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6961 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7021 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7081 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7141 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7201 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7261 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7321 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7381 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7441 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7501 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7561 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     7621 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     7681 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     7741 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     7801 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgrgmkkg
     7861 cwymactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     7921 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
     7981 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccaggg gaggcagaga
     8041 ttgcagtaag ctgagatcgc agcactgcac tccagcctgg gcgacagagt aagactctgt
     8101 ctcaaaaata aaataaataa atcaatcaga tattccaatc ttttccttta tttatttatt
     8161 tattttctat tttggaaaca cagtccttcc ttattccaga attacacata tattctattt
     8221 ttctttatat gctccagttt tttttagacc ttcacctgaa atgtgtgtat acaaaatcta
     8281 ggccagtcca gcagagccta aaggtaaaaa ataaaataat aaaaaataaa taaaatctag
     8341 ctcactcctt cacatcaaaa tggagataca gctgttagca ttaaatacca aataacccat
     8401 cttgtcctca ataattttaa gcgcctctct ccaccacatc taactcctgt caaaggcatg
     8461 tgccccttcc gggcgctctg ctgtgctgcc aaccaactgg catgtggact ctgcagggtc
     8521 cctaactgcc aagccccaca gtgtgccctg aggctgcccc ttccttctag cggctgcccc
     8581 cactcggctt tgctttccct agtttcagtt acttgcgttc agccaaggtc tgaaactagg
     8641 tgcgcacaga gcggtaagac tgcgagagaa agagaccagc tttacagggg gtttatcaca
     8701 gtgcaccctg acagtcgtca gcctcacagg gggtttatca cattgcaccc tgacagtcgt
     8761 cagcctcaca gggggtttat cacagtgcac ccttacaatc attccatttg attcacaatt
     8821 tttttagtct ctactgtgcc taacttgtaa gttaaatttg atcagaggtg tgttcccaga
     8881 ggggaaaaca gtatatacag ggttcagtac tatcgcattt caggcccgag agctgcatcc
     8941 ggagtatcta gatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     9001 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     9061 tagggacttt ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     9121 ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtactctag
     9181 aaggtctata taagcagagc tcgtttagtg aaccgggtct ctctggttag accagatctg
     9241 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     9301 ttgagtgctc aaagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     9361 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     9421 gcgaaagtaa agccagagga gatctctcga cgcaggactc ggcttgctga agcgcgcacg
     9481 gcaagaggcg aggggcggcg actgcagagt acgccaaaaa ttttgactag cggaggctag
     9541 aaggagagag atgggtgcga gagcgtcggt attaagcggg ggagaattag ataaatggga
     9601 aaaaattcgg ttaaggccag ggggaaagaa acaatataaa ctaaaacata tagtatgggc
     9661 aagcagggag ctagaacgat tcgcagttaa tcctggcctt ttagagacat cagaaggctg
     9721 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     9781 attatataat acaatagcag tcctctattg tgtgcatcaa aggatagatg taaaagacac
     9841 caaggaagcc ttagataaga tagaggaaga gcaaaacaaa agtaagaaaa aggcacagca
     9901 agcagcagct gacacaggaa acaacagcca ggtcagccaa aattacccta tagtgcagaa
     9961 cctccagggg caaatggtac atcaggccat atcacctaga actttaaatg catgggtaaa
    10021 agtagtagaa gagaaggctt tcagcccaga agtaataccc atgttttcag cattatcaga
    10081 aggagccacc ccacaagatt taaataccat gctaaacaca gtggggggac atcaagcagc
    10141 catgcaaatg ttaaaagaga ccatcaatga ggaagctgca gaatgggata gattgcatcc
    10201 agtgcatgca gggcctattg caccaggcca gatgagagaa ccaaggggaa gtgacatagc
    10261 aggaactact agcaaattaa gagaacaatt tggaaataat aaaacaataa tctttaagca
    10321 atcctcagga ggggacccag aaattgtaac gcacagtttt aattgtggag gggaattttt
    10381 ctactgtaat tcaacacaac tgtttaatag tacttggttt aatagtactt ggagtactga
    10441 agggtcaaat aacactgaag gaagtgacac aatcacactc ccatgcagaa taaaacaatt
    10501 tataaacatg tggcaggaag taggaaaagc aatgtatgcc cctcccatca gtggacaaat
    10561 tagatgttca tcaaatatta ctgggctgct attaacaaga gatggtggta ataacaacaa
    10621 tgggtccgag atcttcagac ctggaggagg cgatatgagg gacaattgga gaagtgaatt
    10681 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
    10741 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
    10801 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
    10861 attattgtct gatatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
    10921 gcatctgttg caactcacag tctggggcat caaacagctc caggcaagaa tcctggctgt
    10981 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
    11041 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
    11101 gaataacatg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
    11161 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
    11221 agataaatgg gcttgcggcc ggaattcaca aatggcagta ttcatccaca attttaaaag
    11281 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
    11341 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
    11401 cagggacagc agagatccac tttgggc
//
