LOCUS       sequence-311146-       17760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for expression of PULSE system (under the control of 
            CaMV35S promoter) controlling GUS. It constitutively expresses dsRed
            and a plant selection cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17760)
  AUTHORS   Ochoa-Fernandez R, Abel NB, Wieland FG, Schlegel J, Koch LA, Miller 
            JB, Engesser R, Giuriani G, Brandl SM, Timmer J, Weber W, Ott T, 
            Simon R, Zurbriggen MD
  TITLE     Optogenetic control of gene expression in plants in the presence of 
            ambient white light.
  JOURNAL   Nat Methods. 2020 Jul;17(7):717-725. doi: 10.1038/s41592-020-0868-y.
            Epub 2020 Jun 29.
  PUBMED    32601426
REFERENCE   2  (bases 1 to 17760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0868-y"; journalName: "Nat Methods"; date: 
            "2020-07"; volume: "17"; issue: "7"; pages: "717-725"
COMMENT     Sequence Label: BM00369
FEATURES             Location/Qualifiers
     source          1..17760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     548..572
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    2686..2860
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        2961..3306
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3347..3367
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        4378..4723
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             6876..7109
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             7110..7130
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7146..7398
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      9627..9879
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        9983..10328
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             11272..11292
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      11308..11560
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        11663..11842
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             11864..12658
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11918..11937)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12528..12547
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      12671..12923
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    13018..13042
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             13306..13935
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             14364..15437
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      15503..15697
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        15980..16084
                     /gene="bla"
                     /label=AmpR promoter
     CDS             16085..16879
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      17050..17638
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17539..17558
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactccctcg gtcacatgtg
       61 catcctcgct cggagcttcg aagtttaaac gattgaatat aaccgacgtg actgttacat
      121 ttagggattg aatataaccg acgtgactgt tacatttagg gattgaatat aaccgacgtg
      181 actgttacat ttagggattg aatataaccg acgtgactgt tacatttagg gataaacgat
      241 tgaatataac cgacgtgact gttacattta gggattgaat ataaccgacg tgactgttac
      301 atttagggat tgaatataac cgacgtgact gttacattta gggattgaat ataaccgacg
      361 tgactgttac atttagggat gctagcctcg agtaggtagc ctttagtcca tgcgttatag
      421 gtagccttta gtccatgcgt tataggtagc ctttagtcca tgcgttatag gtagccttta
      481 gtccatgcgt tataggtagc ctttagtcca tgaagcttag acactagagc tagccctata
      541 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
      601 acctccatag aagacaccgg gaccgatcca gcctaatggg tcagtccctt atgttacgtc
      661 ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg
      721 atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg
      781 caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt cgtaattatg
      841 cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggtaagtct tactctctct
      901 tttttggtct gtatttttaa ttttttgaag tatactattt gtactgacgc taataatctt
      961 ttttcaggtt gggcaggcca gcgtatcgtg ctgcgtttcg atgcggtcac tcattacggc
     1021 aaagtgtggg tcaataatca ggaagtgatg gagcatcagg gcggctatac gccatttgaa
     1081 gccgatgtca cgccgtatgt tattgccggg aaaagtgtac gtatcaccgt ttgtgtgaac
     1141 aacgaactga actggcagac tatcccgccg ggaatggtga ttaccgacga aaacggcaag
     1201 aaaaagcagt cttacttcca tgatttcttt aactatgccg gaatccatcg cagcgtaatg
     1261 ctctacacca cgccgaacac ctgggtggac gatatcaccg tggtgacgca tgtcgcgcaa
     1321 gactgtaacc acgcgtctgt tgactggcag gtacttcatg cttcaacgtg taacttaaga
     1381 gatactgtgt gaaattttat atttccatac atttgcttga cctttgcttt ttgtcaattt
     1441 ttttcccctt acaggtggtg gccaatggtg atgtcagcgt tgaactgcgt gatgcggatc
     1501 aacaggtggt tgcaactgga caaggcacta gcgggacttt gcaagtggtg aatccgcacc
     1561 tctggcaacc gggtgaaggt tatctctatg aactgtgcgt cacagccaaa agccagacag
     1621 agtgtgatat ctacccgctt cgcgtcggca tccggtcagt ggcagtgaag ggcgaacagt
     1681 tcctgattaa ccacaaaccg ttctacttta ctggctttgg tcgtcatgaa gatgcggact
     1741 tgcgtggcaa aggattcgat aacgtgctga tggtgcacga ccacgcatta atggactgga
     1801 ttggggccaa ctcctaccgt acctcgcatt acccttacgc tgaagagatg ctcgactggg
     1861 cagatgaaca tggcatcgtg gtgattgatg aaactgctgc tgtcggcttt aacctctctt
     1921 taggcattgg tttcgaagcg ggcaacaagc cgaaagaact gtacagcgaa gaggcagtca
     1981 acggggaaac tcagcaagcg cacttacagg cgattaaaga gctgatagcg cgtgacaaaa
     2041 accacccaag cgtggtgatg tggagtattg ccaacgaacc ggatacccgt ccgcaaggtg
     2101 cacgggaata tttcgcgcca ctggcggaag caacgcgtaa actcgacccg acgcgtccga
     2161 tcacctgcgt caatgtaatg ttctgcgacg ctcacaccga taccatcagc gatctctttg
     2221 atgtgctgtg cctgaaccgt tattacggat ggtatgtcca aagcggcgat ttggaaacgg
     2281 cagagaaggt actggaaaaa gaacttctgg cctggcagga gaaactgcat cagccgatta
     2341 tcatcaccga atacggcgtg gatacgttag ccgggctgca ctcaatgtac accgacatgt
     2401 ggagtgaaga gtatcagtgt gcatggctgg atatgtatca ccgcgtcttt gatcgcgtca
     2461 gcgccgtcgt cggtgaacag gtatggaatt tcgccgattt tgcgacctcg caaggcatat
     2521 tgcgcgttgg cggtaacaag aaagggatct tcactcgcga ccgcaaaccg aagtcggcgg
     2581 cttttctgct gcaaaaacgc tggactggca tgaacttcgg tgaaaaaccg cagcagggag
     2641 gcaaacaatg agcttctcta gctagagtcg atcgacaagc tcgagtttct ccataataat
     2701 gtgtgagtag ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga
     2761 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     2821 ctaattccta aaaccaaaat ccagtactaa aatccagatc gctctcgagg aattccacag
     2881 gaggtcaaca tggtggagca cgacactctg gtctactcca aaaatgtcaa agatacagtc
     2941 tcagaagatc aaagggctat tgagactttt caacaaagga taatttcggg aaacctcctc
     3001 ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc
     3061 tcctacaaat gccatcattg cgataaagga aaggctatca ttcaagatct ctctgccgac
     3121 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agaggttcca
     3181 accacgtcta caaagcaagt ggattgatgt gacatctcca ctgacgtaag ggatgacgca
     3241 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
     3301 aggacacgca atgatgcttg atcttgaact tagacttggt tttgctccaa agaagaagag
     3361 gaaggtggga gctggagctg gggcagacga cacacgcgtt gaggtgcaac cgccggcgca
     3421 gtgggtcctc gacctgatcg aggccagccc gatcgcatcg gtcgtgtccg atccgcgtct
     3481 cgccgacaat ccgctgatcg ccatcaacca ggccttcacc gacctgaccg gctattccga
     3541 agaagaatgc gtcggccgca attgccgatt cctggcaggt tccggcaccg agccgtggct
     3601 gaccgacaag atccgccaag gcgtgcgcga gcacaagccg gtgctggtcg agatcctgaa
     3661 ctacaagaag gacggcacgc cgttccgcaa tgccgtgctc gttgcaccga tctacgatga
     3721 cgacgacgag cttctctatt tcctcggcag ccaggtcgaa gtcgacgacg accagcccaa
     3781 catgggcatg gcgcgccgcg aacgcgccgc ggaaatgctc aagacgctgt cgccgcgcca
     3841 gctcgaggtt acgacgctgg tggcatcggg cttgcgcaac aaggaagtgg cggcccggct
     3901 cggcctgtcg gagaaaaccg tcaagatgca ccgcgggctg gtgatggaaa agctcaacct
     3961 gaagaccagt gccgatctgg tgcgcattgc cgtcgaagcc ggaatctaag cttgtcaagc
     4021 agatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgacgct gcatgcgaat tctctgggag gtcaacatgg tggagcacga
     4321 cactctggtc tactccaaaa atgtcaaaga tacagtctca gaagatcaaa gggctattga
     4381 gacttttcaa caaaggataa tttcgggaaa cctcctcgga ttccattgcc cagctatctg
     4441 tcacttcatc gaaaggacag tagaaaagga aggtggctcc tacaaatgcc atcattgcga
     4501 taaaggaaag gctatcattc aagatctctc tgccgacagt ggtcccaaag atggaccccc
     4561 acccacgagg agcatcgtgg aaaaagaaga ggttccaacc acgtctacaa agcaagtgga
     4621 ttgatgtgac atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
     4681 cccttcctct atataaggaa gttcatttca tttggagagg acacgcaatg acaacaatgg
     4741 tttccggagt cgggggtagt ggcggtggcc gtggcggtgg ccgtggcgga gaagaagaac
     4801 cgtcgtcaag tcacactcct aataaccgaa gaggaggaga acaagctcaa tcgtcgggaa
     4861 cgaaatctct cagaccaaga agcaacactg aatcaatgag caaagcaatt caacagtaca
     4921 ccgtcgacgc aagactccac gccgttttcg aacaatccgg cgaatcaggg aaatcattcg
     4981 actactcaca atcactcaaa acgacgacgt acggttcctc tgtacctgag caacagatca
     5041 cagcttatct ctctcgaatc cagcgaggtg gttacattca gcctttcgga tgtatgatcg
     5101 ccgtcgatga atccagtttc cggatcatcg gttacagtga aaacgccaga gaaatgttag
     5161 ggattatgcc tcaatctgtt cctactcttg agaaacctga gattctagct atgggaactg
     5221 atgtgagatc tttgttcact tcttcgagct cgattctact cgagcgtgct ttcgttgctc
     5281 gagagattac cttgttaaat ccggtttgga tccattccaa gaatactggt aaaccgtttt
     5341 acgccattct tcataggatt gatgttggtg ttgttattga tttagagcca gctagaactg
     5401 aagatcctgc gctttctatt gctggtgctg ttcaatcgca gaaactcgcg gttcgtgcga
     5461 tttctcagtt acaggctctt cctggtggag atattaagct tttgtgtgac actgtcgtgg
     5521 aaagtgtgag ggacttgact ggttatgatc gtgttatggt ttataagttt catgaagatg
     5581 agcatggaga agttgtagct gagagtaaac gagatgattt agagccttat attggactgc
     5641 attatcctgc tactgatatt cctcaagcgt caaggttctt gtttaagcag aaccgtgtcc
     5701 gaatgatagt agattgcaat gccacacctg ttcttgtggt ccaggacgat aggctaactc
     5761 agtctatgtg cttggttggt tctactctta gggctcctca tggttgtcac tctcagtata
     5821 tggctaacat gggatctatt gcgtctttag caatggcggt tataatcaat ggaaatgaag
     5881 atgatgggag caatgtagct agtggaagaa gctcgatgag gctttggggt ttggttgttt
     5941 gccatcacac ttcttctcgc tgcataccgt ttccgctaag gtatgcttgt gagtttttga
     6001 tgcaggcttt cggtttacag ttaaacatgg aattgcagtt agctttgcaa atgtcagaga
     6061 aacgcgtttt gagaacgcag acactgttat gtgatatgct tctgcgtgac tcgcctgctg
     6121 gaattgttac acagagtccc agtatcatgg acttagtgaa atgtgacggt gcagcatttc
     6181 tttaccacgg gaagtattac ccgttgggtg ttgctcctag tgaagttcag ataaaagatg
     6241 ttgtggagtg gttgcttgcg aatcatgcgg attcaaccgg attaagcact gatagtttag
     6301 gcgatgcggg gtatcccggt gcagctgcgt taggggatgc tgtgtgcggt atggcagttg
     6361 catatatcac aaaaagagac tttctttttt ggtttcgatc tcacactgcg aaagaaatca
     6421 aatggggagg cgctaagcat catccggagg ataaagatga tgggcaacga atgcatcctc
     6481 gttcgtcctt tcaggctttt cttgaagttg ttaagagccg gagtcagcca tgggaaactg
     6541 cggaaatgga tgcgattcac tcgctccagc ttattctgag agactctttt aaagaatctg
     6601 aggcggctat gaactctaaa gttgtggatg gtgtggttca gccatgtagg gatatggcgg
     6661 gggaacaggg gattgatgag ttaggtgaat tcgatagtgc tggtagtgct ggtagtgctg
     6721 gttccgcgta cagccgcgcg cgtacgaaaa acaattacgg gtctaccatc gagggcctgc
     6781 tcgatctccc ggacgacgac gcccccgaag aggcggggct ggcggctccg cgcctgtcct
     6841 ttctccccgc gggacacacg cgcagactgt cgacggcccc cccgaccgat gtcagcctgg
     6901 gggacgagct ccacttagac ggcgaggacg tggcgatggc gcatgccgac gcgctagacg
     6961 atttcgatct ggacatgttg ggggacgggg attccccggg tccgggattt accccccacg
     7021 actccgcccc ctacggcgct ctggatatgg ccgacttcga gtttgagcag atgtttaccg
     7081 atgcccttgg aattgacgag tacggtgggc caaagaagaa gaggaaggtg taggcttgtc
     7141 aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     7201 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     7261 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     7321 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     7381 catctatgtt actagatcga cgctccatgg gaattcgtaa ggaggacgtc gttgtggttg
     7441 gtgctttcct tacattctga gcctctttcc ttctaatcca ctcatctgca tcttcttgtg
     7501 tccttactaa tacctcattg gttccaaatt ccctcccttt aagcaccagc tcgtttctgt
     7561 tcttccacag cctcccaagt atccaaggga ctaaagcctc cacattcttc agatcaggat
     7621 attcttgttt aagatgttga actctatgga ggtttgtatg aactgatgat ctaggaccgg
     7681 ataagttccc ttcttcatag cgaacttatt caaagaatgt tttgtgtatc attcttgtta
     7741 cattgttatt aatgaaaaaa tattattggt cattggactg aacacgagtg ttaaatatgg
     7801 accaggcccc aaataagatc cattgatata tgaattaaat aacaagaata aatcgagtca
     7861 ccaaaccact tgcctttttt aacgagactt gttcaccaac ttgatacaaa agtcattatc
     7921 ctatgcaaat caataatcat acaaaaatat ccaataacac taaaaaaatt aaaagaaatg
     7981 gataatttca caatatgtta tacgataaag aagttacttt tccaagaaat tcactgattt
     8041 tataagccca cttgcattag ataaatggca aaaaaaaaca aaaaggaaaa gaaataaagc
     8101 acgaagaatt ctagaaaata cgaaatacgc ttcaatgcag tgggacccac ggttcaatta
     8161 ttgccaattt tcagctccac cgtatattta aaaaataaaa cgataatgct aaaaaaatat
     8221 aaatcgtaac gatcgttaaa tctcaacggc tggatcttat gacgaccgtt aaggaaattg
     8281 tggttgtcgg acgaagtcca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg
     8341 agtcgtgttt atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta
     8401 gccaaaaaca actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta
     8461 ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt
     8521 ctataaaaca atacccaaag agctcttctt cttcacaatt cagatttcaa tttctcaaaa
     8581 tcttaaaaac tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta
     8641 ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt
     8701 ttagattctg ttaatcttag atcgtagacg attttctggg tttgatcgtt agatatcatc
     8761 ttaattctcg attagggttt catagatatc atccgatttg ttcaaataat ttgagttttg
     8821 tcgaataatt actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg
     8881 tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta acagatgcag atcttaatgg
     8941 ggtcatccaa gaatgttatc aaggagttca tgaggtttaa ggttcgcatg gaaggaacgg
     9001 tcaatgggca cgagtttgaa atagaaggcg aaggagaggg gaggccatac gaaggccaca
     9061 ataccgtaaa gcttaaggta accaaggggg gacctttgcc atttgcttgg gatattttgt
     9121 caccacaatt tcagtatgga agcaaggtat atgtcaagca ccctgccgac ataccagact
     9181 ataaaaagct gtcatttcct gaaggattta aatgggaaag ggtcatgaac tttgaagatg
     9241 gtggcgtcgt tactgtaacc caggattcca gtttgcagga tggctgtttc atctacaagg
     9301 tcaagttcat tggcgtgaac tttccttccg atggacctgt tatgcaaaag aaaacaatgg
     9361 gctgggaagc cagcactgag cgtttgtatc ctcgtgatgg cgtgttgaaa ggagagattc
     9421 ataaggctct gaagctgaaa gacggtggtc attacctagt tgaattcaaa agtatttaca
     9481 tggcaaagaa gcctgtgcag ctaccagggt actactatgt tgactccaaa ctggatataa
     9541 caagccacaa cgaagattat acaatcgttg agcagtatga aagaaccgag ggacgccacc
     9601 atctgttcct ttaagcttgt caagcagatc gttcaaacat ttggcaataa agtttcttaa
     9661 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     9721 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     9781 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     9841 ataaattatc gcgcgcggtg tcatctatgt tactagatcg acgctgagct cgaattctag
     9901 tggaggtcaa catggtggag cacgacactc tggtctactc caaaaatgtc aaagatacag
     9961 tctcagaaga tcaaagggct attgagactt ttcaacaaag gataatttcg ggaaacctcc
    10021 tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaaggtg
    10081 gctcctacaa atgccatcat tgcgataaag gaaaggctat cattcaagat ctctctgccg
    10141 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagaggttc
    10201 caaccacgtc tacaaagcaa gtggattgat gtgacatctc cactgacgta agggatgacg
    10261 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
    10321 agaggacacg caatgacaac aatgccccgc cccaagctca agtccgatga cgaggtactc
    10381 gaggccgcca ccgtagtgct gaagcgttgc ggtcccatag agttcacgct cagcggagta
    10441 gcaaaggagg tggggctctc ccgcgcagcg ttaatccagc gcttcaccaa ccgcgatacg
    10501 ctgctggtga ggatgatgga gcgcggcgtc gagcaggtgc ggcattacct gaatgcgata
    10561 ccgataggcg cagggccgca agggctctgg gaatttttgc aggtgctcgt tcggagcatg
    10621 aacactcgca acgacttctc ggtgaactat ctcatctcct ggtacgagct ccaggtgccg
    10681 gagctacgca cgcttgcgat ccagcggaac cgcgcggtgg tggaggggat ccgcaagcga
    10741 ctgcccccag gtgctcctgc ggcagctgag ttgctcctgc actcggtcat cgctggcgcg
    10801 acgatgcagt gggccgtcga tccggatggt gagctagctg atcatgtgct ggctcagatc
    10861 gctgccatcc tgtgtttaat gtttcccgaa cacgacgatt tccaactcct ccaggcacat
    10921 gcgtccgcgt acagcttaat taacagtgct ggtagtgctg gtagtgctgg tatgatgttc
    10981 ttaccaaccg attattgttg caggttaagc gatcaagagt atatggagct tgtgtttgag
    11041 aatggccaga ttcttgcaaa gggccaaaga tccaacgttt ctctgcataa tcaacgtacc
    11101 aaatcgatca tggatttgta tgaggcagag tataacgagg atttcatgaa gagtatcatc
    11161 catggtggtg gtggtgccat cacaaatctc ggggacacgc aggttgttcc acaaagtcat
    11221 gttgctgctg cccatgaaac aaacatgttg gaaagcaata aacatgttga cccaaagaag
    11281 aagaggaagg tgtaggcttg tcaagcagat cgttcaaaca tttggcaata aagtttctta
    11341 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
    11401 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
    11461 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
    11521 gataaattat cgcgcgcggt gtcatctatg ttactagatc gacgctaagc ttgaattctt
    11581 gcggagagcg gagaattaag ggagtcacgt tatgaccccc gccgatgacg cgggacaagc
    11641 cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactcag ccgcgggttt
    11701 ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc
    11761 ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata
    11821 aattcccctc ggtatccaat tagagtctca tattcactct caaatgattg aacaagatgg
    11881 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
    11941 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
    12001 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctccaagacg aggcagcgcg
    12061 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
    12121 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
    12181 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
    12241 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gaggacgtac
    12301 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
    12361 gccagccgaa ctgttcgcca ggctcaaggc gcggatgccc gacggcgagg atctcgtcgt
    12421 gacccagggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
    12481 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
    12541 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
    12601 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
    12661 ttgtcaagca gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
    12721 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
    12781 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
    12841 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
    12901 ggtgtcatct atgttactag atcgacgctg gatccgaatt cggcagggcc cactgcatcc
    12961 accccagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
    13021 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
    13081 aaaatcacca ctcgatacag gcagcccatc agccgtgcgg ctgcatgaaa tcctggccgg
    13141 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg
    13201 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
    13261 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
    13321 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc
    13381 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
    13441 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
    13501 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
    13561 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
    13621 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
    13681 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
    13741 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
    13801 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
    13861 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
    13921 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
    13981 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
    14041 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
    14101 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc
    14161 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
    14221 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
    14281 acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga
    14341 acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg
    14401 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
    14461 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
    14521 gcaaagaatc ccggcaaccg ccggcagccg gtgcgtcgtc gattaggaag ccgcccaagg
    14581 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
    14641 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
    14701 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
    14761 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
    14821 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
    14881 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
    14941 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
    15001 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
    15061 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
    15121 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
    15181 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
    15241 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
    15301 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
    15361 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
    15421 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
    15481 aaggtcgaaa aagcttcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
    15541 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
    15601 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
    15661 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
    15721 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
    15781 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
    15841 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccg
    15901 agctcccatc cccccagggg ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg
    15961 cagcgctggc caattcgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    16021 tatgtatccg ctcatgagac aataaccttg ataaatgctt caataatatt gaaaaaggaa
    16081 gagtatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
    16141 cgcaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
    16201 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
    16261 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
    16321 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
    16381 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
    16441 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
    16501 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
    16561 ggaagaggac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
    16621 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
    16681 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
    16741 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
    16801 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
    16861 actggatgaa ttgttttagc tgtcagacca agtttactca tatatacttt agattgattt
    16921 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    16981 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    17041 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    17101 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    17161 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
    17221 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    17281 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    17341 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    17401 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    17461 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    17521 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    17581 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    17641 cgccagcaac gcggcctttt tacggttcct gtggttggca catacaaatg gacgaacgga
    17701 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
//
