LOCUS       sequence-311153-        5539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a rabbit Fc-ICAM1 fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Reimer D, Meyer-Hermann M, Rakhymzhan A, Steinmetz T, Tripal P, 
            Thomas J, Boettcher M, Mougiakakos D, Schulz SR, Urbanczyk S, Hauser
            AE, Niesner RA, Mielenz D
  TITLE     B Cell Speed and B-FDC Contacts in Germinal Centers Determine Plasma
            Cell Output via Swiprosin-1/EFhd2.
  JOURNAL   Cell Rep. 2020 Aug 11;32(6):108030. doi: 
            10.1016/j.celrep.2020.108030.
  PUBMED    32783949
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-08-11"; volume: "32(6):108030. doi"; pages: " 
            10.1016/j.celrep.2020.108030"
COMMENT     Sequence Label: pFUSE-rIgG-Fc2-ICAM1
FEATURES             Location/Qualifiers
     source          1..5539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        49..260
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             273..541
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     591..650
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     polyA_signal    2722..2843
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2733..2752)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2787..2806
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    2947..3341
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(3355..3729)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        4322..4625
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(4705..5293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4785..4804)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5401..5449
                     /note="synthetic polyadenylation signal"
     misc_feature    join(5460..5539,1..12)
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5500..5519
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 gaacatttct ctatcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca
       61 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga
      121 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
      181 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
      241 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc
      301 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct
      361 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg
      421 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc
      481 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat
      541 ccaagctgtg accggcgcct acctgagatc accggcgaag gagggccacc atgtacagga
      601 tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg caggtatcca
      661 tccatcccag agaagccttc ctgccccagg gtgggtccgt gcaggtgaac tgttcttcct
      721 catgcaagga ggacctcagc ctgggcttgg agactcagtg gctgaaagat gagctcgaga
      781 gtggatccaa ctggaagctg tttgagctga gcgagatcgg ggaggacagc agtccgctgt
      841 gctttgagaa ctgtggcacc gtgcagtcgt ccgcttccgc taccatcacc gtgtattcgt
      901 ttccggagag tgtggagctg agacctctgc cagcctggca gcaagtaggc aaggacctca
      961 ccctgcgctg ccacgtggat ggtggagcac cgcggaccca gctctcagca gtgctgctcc
     1021 gtggggagga gatactgagc cgccagccag tgggtgggca ccccaaggac cccaaggaga
     1081 tcacattcac ggtgctggct agcagagggg accacggagc caatttctca tgccgcacag
     1141 aactggatct caggccgcaa gggctggcat tgttctctaa tgtctccgag gccaggagcc
     1201 tccggacttt cgatcttcca gctaccatcc caaagctcga cacccctgac ctcctggagg
     1261 tgggcaccca gcagaagttg ttttgctccc cggaaggcct gtttcctgcc tctgaagctc
     1321 ggatatacct ggagctggga ggccagatgc cgacccagga gagcacaaac agcagtgact
     1381 ctgtgtcagc cactgccttg gtagaggtga ctgaggagtt cgacagaacc ctgccgctgc
     1441 gctgcgtttt ggagctagcg gaccagatcc tggagacgca gaggacctta acagtctaca
     1501 acttttcagc tccggtcctg accctgagcc agctggaggt ctcggaaggg agccaagtaa
     1561 ctgtgaagtg tgaagcccac agtgggtcga aggtggttct tctgagcggc gtcgagccta
     1621 ggccacccac cccgcaggtc caattcacac tgaatgccag ctcggaggat cacaaacgaa
     1681 gcttcttttg ctctgccgct ctggaggtgg cgggaaagtt cctgtttaaa aaccagaccc
     1741 tggaactgca cgtgctgtat ggtcctcggc tggacgagac ggactgcttg gggaactgga
     1801 cctggcaaga ggggtctcag cagactctga aatgccaggc ctgggggaac ccatctccta
     1861 agatgacctg cagacggaag gcagatggtg ccctgctgcc catcggggtg gtgaagtctg
     1921 tcaaacagga gatgaatggt acatacgtgt gccatgcctt tagctcccat gggaatgtca
     1981 ccaggaatgt gtacctgaca gtactgtacc actctcaaaa taacagatct agcaagccca
     2041 cgtgcccacc ccctgaactc ctggggggac cgtctgtctt catcttcccc ccaaaaccca
     2101 aggacaccct catgatctca cgcacccccg aggtcacatg cgtggtggtg gacgtgagcc
     2161 aggatgaccc cgaggtgcag ttcacatggt acataaacaa cgagcaggtg cgcaccgccc
     2221 ggccgccgct acgggagcag cagttcaaca gcacgatccg cgtggtcagc accctcccca
     2281 tcgcgcacca ggactggctg aggggcaagg agttcaagtg caaagtccac aacaaggcac
     2341 tcccggcccc catcgagaaa accatctcca aagccagagg gcagcccctg gagccgaagg
     2401 tctacaccat gggccctccc cgggaggagc tgagcagcag gtcggtcagc ctgacctgca
     2461 tgatcaacgg cttctaccct tccgacatct cggtggagtg ggagaagaac gggaaggcag
     2521 aggacaacta caagaccacg ccggccgtgc tggacagcga cggctcctac ttcctctaca
     2581 gcaagctctc agtgcccacg agtgagtggc agcggggcga cgtcttcacc tgctccgtga
     2641 tgcacgaggc cttgcacaac cactacacgc agaagtccat ctcccgctct ccgggtaaat
     2701 gagctagctg gccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     2761 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     2821 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     2881 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggaat
     2941 taattctaaa atacagcata gcaaaacttt aacctccaaa tcaagcctct acttgaatcc
     3001 ttttctgagg gatgaataag gcataggcat caggggctgt tgccaatgtg cattagctgt
     3061 ttgcagcctc accttctttc atggagttta agatatagtg tattttccca aggtttgaac
     3121 tagctcttca tttctttatg ttttaaatgc actgacctcc cacattccct ttttagtaaa
     3181 atattcagaa ataatttaaa tacatcattg caatgaaaat aaatgttttt tattaggcag
     3241 aatccagatg ctcaaggccc ttcataatat cccccagttt agtagttgga cttagggaac
     3301 aaaggaacct ttaatagaaa ttggacagca agaaagcgag cttctagctt atcctcagtc
     3361 ctgctcctct gccacaaagt gcacgcagtt gccggccggg tcgcgcaggg cgaactcccg
     3421 cccccacggc tgctcgccga tctcggtcat ggccggcccg gaggcgtccc ggaagttcgt
     3481 ggacacgacc tccgaccact cggcgtacag ctcgtccagg ccgcgcaccc acacccaggc
     3541 cagggtgttg tccggcacca cctggtcctg gaccgcgctg atgaacaggg tcacgtcgtc
     3601 ccggaccaca ccggcgaagt cgtcctccac gaagtcccgg gagaacccga gccggtcggt
     3661 ccagaactcg accgctccgg cgacgtcgcg cgcggtgagc accggaacgg cactggtcaa
     3721 cttggccatg atggctcctc ctgtcaggag aggaaagaga agaaggttag tacaattgct
     3781 atagtgagtt gtattatact atgcagatat actatgccaa tgattaattg tcaaactagg
     3841 gctgcagggt tcatagtgcc acttttcctg cactgcccca tctcctgccc accctttccc
     3901 aggcatagac agtcagtgac ttaccaaact cacaggaggg agaaggcaga agcttgagac
     3961 agacccgcgg gaccgccgaa ctgcgagggg acgtggctag ggcggcttct tttatggtgc
     4021 gccggccctc ggaggcaggg cgctcgggga ggcctagcgg ccaatctgcg gtggcaggag
     4081 gcggggccga aggccgtgcc tgaccaatcc ggagcacata ggagtctcag ccccccgccc
     4141 caaagcaagg ggaagtcacg cgcctgtagc gccagcgtgt tgtgaaatgg gggcttgggg
     4201 gggttggggc cctgactagt caaaacaaac tcccattgac gtcaatgggg tggagacttg
     4261 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     4321 tcatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     4381 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     4441 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     4501 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     4561 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     4621 taacgcctgc aggttaatta agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4681 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4741 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4801 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4861 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4921 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4981 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5041 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5101 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     5161 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5221 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5281 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5341 aaactcacgt taagggattt tggtcatggc tagttaatta acatttaaat cagcggccgc
     5401 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgtaacta
     5461 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
     5521 agtgcaagtg caggtgcca
//
