LOCUS       sequence-311154-        7497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express a bright monomeric red FP for labelling and detection of 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7497)
  AUTHORS   Unpublished
  TITLE     Pantelis Tsoulfas Lab plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-mScarlet-I
FEATURES             Location/Qualifiers
     source          1..7497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    115..231
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        254..557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        558..761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     712..732
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     758..782
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      866..875
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1654..2242
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1707..1727)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2125..2136)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2329..2562
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2634..2755
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2671..2690)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2725..2744
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2795..2930
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2950..2969)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2951..2969)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2979..2996)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2979..2995)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2988..3010)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3137..3592
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3224..3243)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3434..3455
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3618..3722
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3723..3791,3792..4583)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3941..3960)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4754..5342
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5243..5262
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5496..5513
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5630..5651
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5666..5671,5672..5689,5690..5696)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5704..5720
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5709..5731
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5728..5744
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5728..5744
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5763..5783
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5765..5783
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5811..6037
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6038..6218
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6265..6390
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6883..7116
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7301..7345
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
       61 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
      121 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
      181 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgataag
      241 cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      421 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      481 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      541 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      661 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
      781 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgc ccgggcgtac
      841 gggatcctct agaaagcttg aattcgccac catggtttcc aagggcgaag ccgtgatcaa
      901 agagttcatg cggttcaagg tgcacatgga aggcagcatg aacggccacg agttcgagat
      961 cgaaggcgaa ggcgagggca gaccttatga gggaacacag accgccaagc tgaaagtgac
     1021 caaaggcggc cctctgcctt tcagctggga cattctgagc cctcagttta tgtacggcag
     1081 ccgggccttc atcaagcacc ctgccgatat tcccgactac tacaagcaga gcttccccga
     1141 gggcttcaag tgggagagag tgatgaactt cgaggacggc ggagccgtga ccgtgacaca
     1201 ggatacaagc ctggaagatg gcaccctgat ctacaaagtg aagctgcggg gcaccaactt
     1261 tccacctgat ggccccgtga tgcagaaaaa gaccatgggc tgggaagcca gcaccgagag
     1321 actgtatcct gaggatggcg tgctgaaggg cgacatcaag atggccctga gactgaagga
     1381 tggcggcaga tacctggccg acttcaagac cacctacaag gccaagaaac ccgtgcagat
     1441 gcctggcgcc tacaacgtgg acagaaagct ggacatcacc agccacaacg aggactacac
     1501 cgtggtggaa cagtacgagc ggagcgaagg cagacactct acaggcggaa tggacgagct
     1561 gtacaagtga ctcgagatgc atactagtga attcacgcgt tctagacctg cagggtcgac
     1621 tccggatgat cagggccctg tacagatatc gacaatcaac ctctggatta caaaatttgt
     1681 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     1741 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     1801 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     1861 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     1921 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     1981 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     2041 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     2101 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     2161 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     2221 tccctttggg ccgcctcccc gcctggaatt cgagctcggt acctttaaga ccaatgactt
     2281 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     2341 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     2401 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     2461 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     2521 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     2581 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     2641 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2701 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     2761 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     2821 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     2881 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     2941 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     3001 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     3061 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     3121 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3181 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3241 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3301 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3361 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3421 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     3481 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     3541 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     3601 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     3661 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3721 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     3781 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     3841 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     3901 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     3961 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4021 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4081 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4141 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     4201 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     4261 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     4321 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     4381 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     4441 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4501 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     4561 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     4621 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4681 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4741 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4801 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4861 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     4921 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4981 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5041 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5101 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5161 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5221 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5281 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5341 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5401 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5461 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5521 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     5581 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     5641 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     5701 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     5761 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     5821 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     5881 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     5941 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     6001 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     6061 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6121 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6181 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     6241 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     6301 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     6361 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     6421 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     6481 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     6541 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     6601 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     6661 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     6721 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     6781 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     6841 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     6901 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     6961 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     7021 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     7081 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     7141 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     7201 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     7261 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     7321 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     7381 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     7441 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agaccca
//
