LOCUS       sequence-311163-       10023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A mutational reporter plasmid that can gain Kanamycin and/or 
            Tetracycline resistance upon mutation. Contains T7 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   Moore CL, Papa LJ 3rd, Shoulders MD
  TITLE     A Processive Protein Chimera Introduces Mutations across Defined DNA
            Regions In Vivo.
  JOURNAL   J Am Chem Soc. 2018 Sep 19;140(37):11560-11564. doi: 
            10.1021/jacs.8b04001. Epub 2018 Jul 18.
  PUBMED    29991261
REFERENCE   2  (bases 1 to 10023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.8b04001"; journalName: "J Am Chem Soc"; date: 
            "2018-09-19- 19"; volume: "140"; issue: "37"; pages: "11560-11564"
COMMENT     Sequence Label: T7 promoter + filler DNA reporter plasmid
FEATURES             Location/Qualifiers
     source          1..10023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        63..110
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             132..932
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(192..211)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     802..821
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(1023..2672)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     promoter        join(2787..2792,2793..2809,2810..2815)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             2861..4063
                     /codon_start=1
                     /gene="tetA from transposon Tn10"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="NSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFGVL
                     LALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRLLSG
                     ITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSPFFIA
                     ALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYFSAQLI
                     GQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKTAVLLGF
                     IADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGALQGLLVSL
                     TNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQAQGSKQETS
                     A"
     primer_bind     complement(4089..4107)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    4388..4861
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(4934..5905)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(5905..7080)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    7406..7656
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(7659..8414)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      8505..8724
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             complement(join(8950..9741,9742..9810))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9573..9592
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9811..9915)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taatacgact cactataggg agacccaagc tggcttgaca attaatcatc ggctcgaagc
       61 ttgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
      121 actaaaccac gggatcggcc attgaacaag atggattgca cgcaggttct ccggccgctt
      181 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
      241 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
      301 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
      361 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
      421 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
      481 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
      541 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
      601 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
      661 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
      721 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
      781 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
      841 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
      901 ccttctatcg ccttcttgac gagttcttct gaggggatca attctctaga gcttaattaa
      961 cgcagcctga atggcgaata gaagtttaaa cgctagattg gacaatggcg agcctagtct
     1021 cccacggcga tcttgccgcc cttcttggcc ttaatgagaa tctcgcggat cttgcgggcg
     1081 tccaacttgc cggtcagtcc tttaggcacc tcgtccacga acacaacacc accgcgcagc
     1141 ttcttggcgg ttgtaacctg gctggccaca tagtccacga tctccttctc ggtcatggtt
     1201 ttaccgtgtt ccagcacgac gactgcggcg ggcagctcgc cggcatcgtc gtcgggcagg
     1261 ccggcgaccc cggcgtcgaa gatgttgggg tgttgcagca ggatgctctc cagttcggct
     1321 ggggctacct ggtagccctt gtatttgatc aggctcttca gccggtccac gatgaagaag
     1381 tgctcgtcct cgtcccagta ggcgatgtcg ccgctgtgca gccagccgtc cttgtcgatg
     1441 agagcgtttg tagcctcggg gttgttaacg tagccgctca tgatcatggg gccacggacg
     1501 cacagctcgc cgcgctggtt cacacccagt gtcttaccgg tgtccaagtc caccacctta
     1561 gcctcgaaga agggcaccac cttgcctact gcgccaggct tgtcgtcccc ttcgggggtg
     1621 atcagaatgg cgctggttgt ttctgtcagg ccgtagccct ggcggatgcc tggtaggtgg
     1681 aagcgtttgg ccacggcctc acctacctcc ttgctgagcg gcgccccgcc gctggcgatc
     1741 tcgtgcaagt tgcttaggtc gtacttgtcg atgagagtgc tcttagcgaa gaagctaaat
     1801 agtgtgggca ccagcagggc agattgaatc ttatagtctt gcaagctgcg caagaatagc
     1861 tcctcctcga agcggtacat gagcacgacc cgaaagccgc agatcaagta gcccagcgtg
     1921 gtgaacatgc cgaagccgtg gtgaaatggc accacgctga ggatagcggt gtcggggatg
     1981 atctggttgc cgaagatggg gtcgcgggca tgactgaatc ggacacaagc ggtgcggtgc
     2041 ggtagggcta cgcccttggg caatccggta ctgccactac tgttcatgat cagggcgatg
     2101 gttttgtccc ggtcgaagct ctcgggcacg aagtcgtact cgttgaagcc gggtggcaaa
     2161 tgggaagtca cgaaggtgta catgctttgg aagccctggt agtcggtctt gctatccatg
     2221 atgatgatct tttgtatgat cggtagcttc ttttgcacgt tgaggatctt ttgcagccct
     2281 ttcttgctca cgaatacgac ggtgggctgg ctgatgccca tgctgttcag cagctcgcgc
     2341 tcgttgtaga tgtcgttagc tggggccaca gccacaccga tgaacagggc acccaacacg
     2401 ggcatgaaga actgcaagct attctcgctg cacaccacga tccgatggtt tgtattcagc
     2461 ccatagcgct tcatagcttc tgccagccga acgctcatct cgaagtactc ggcgtaggta
     2521 atgtccacct cgatatgtgc gtcggtaaag gcgatggtgc cgggcaccag ggcgtagcgc
     2581 ttcatggctt tgtgcagctg ctcgccggcg gtcccgtctt cgagtgggta gaatggcgct
     2641 gggcccttct taatgttttt ggcatcttcc atggtggtga attccaccac actggactag
     2701 tggatcctag ggatgttttg gctccatatg atcactacaa agacaccaga acaggtgttg
     2761 tagttggacc agattccaac cgatccttga cagcttatca tcgataagct ttaatgcggt
     2821 agtttatcac agttgctaac gcagtcaggc accgtgtacg aatagttcga caaagatcgc
     2881 attggtaatt acgttactcg atgccatggg gattggcctt atcatgccag tcttgccaac
     2941 gttattacgt gaatttattg cttcggaaga tatcgctaac cactttggcg tattgcttgc
     3001 actttatgcg ttaatgcagg ttatctttgc tccttggctt ggaaaaatgt ctgaccgatt
     3061 tggtcggcgc ccagtgctgt tgttgtcatt aataggcgca tcgctggatt acttattgct
     3121 ggctttttca agtgcgcttt ggatgctgta tttaggccgt ttgctttcag ggatcacagg
     3181 agctactggg gctgtcgcgg catcggtcat tgccgatacc acctcagctt ctcaacgcgt
     3241 gaagtggttc ggttggttag gggcaagttt tgggcttggt ttaatagcgg ggcctattat
     3301 tggtggtttt gcaggagaga tttcaccgca tagtcccttt tttatcgctg cgttgctaaa
     3361 tattgtcact ttccttgtgg ttatgttttg gttccgtgaa accaaaaata cacgtgataa
     3421 tacagatacc gaagtagggg ttgagacgca atcgaattcg gtatacatca ctttatttaa
     3481 aacgatgccc attttgttga ttatttattt ttcagcgcaa ttgataggcc aaattcccgc
     3541 aacggtgtgg gtgctattta ccgaaaatcg ttttggatgg aatagcatga tggttggctt
     3601 ttcattagcg ggtcttggtc ttttacactc agtattccaa gcctttgtgg caggaagaat
     3661 agccactaaa tggggcgaaa aaacggcagt actgctcgga tttattgcag atagtagtgc
     3721 atttgccttt ttagcgttta tatctgaagg ttggttagtt ttccctgttt taattttatt
     3781 ggctggtggt gggatcgctt tacctgcatt acagggagtg atgtctatcc aaacaaagag
     3841 tcatcagcaa ggtgctttac agggattatt ggtgagcctt accaatgcaa ccggtgttat
     3901 tggcccatta ctgtttgctg ttatttataa tcattcacta ccaatttggg atggctggat
     3961 ttggattatt ggtttagcgt tttactgtat tattatcctg ctatcgatga ccttcatgtt
     4021 aacccctcaa gctcagggga gtaaacagga gacaagtgct tagtgatcca attcttgaag
     4081 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4141 ttagacgtca ggtggcactt ttcggggaaa tgtgcccggt taacgtgccg gcacggcctg
     4201 ggtaaccagg tattttgtcc acataaccgt gcgcaaaatg ttgtggataa gcaggacaca
     4261 gcagcaatcc acagcaggca tacaaccgca caccgaggtt actccgttct acaggttacg
     4321 acgacatgtc aatacttgcc cttgacaggc attgatggaa tcgtagtctc acgctgatag
     4381 tctgatcgac aatacaagtg ggaccgtggt cccagaccga taatcagacc gacaacacga
     4441 gtgggatcgt ggtcccagac taataatcag accgacgata cgagtgggac cgtggtccca
     4501 gactaataat cagaccgacg atacgagtgg gaccgtggtt ccagactaat aatcagaccg
     4561 acgatacgag tgggaccgtg gtcccagact aataatcaga ccgacgatac gagtgggacc
     4621 atggtcccag actaataatc agaccgacga tacgagtggg accgtggtcc cagtctgatt
     4681 atcagaccga cgatacgagt gggaccgtgg tcccagacta ataatcagac cgacgatacg
     4741 agtgggaccg tggtcccaga ctaataatca gaccgacgat acgagtggga ccgtggtccc
     4801 agtctgatta tcagaccgac gatacaagtg gaacagtggg cccagagaga atattcaggc
     4861 cagttatgct ttctggcctg taacaaagga cattaagtaa agacagataa acgtagacta
     4921 aaacgtggtc gcatcagggt gctggctttt caagttcctt aagaatggcc tcaattttct
     4981 ctatacactc agttggaaca cgagacctgt ccaggttaag caccatttta tcgcccttat
     5041 acaatactgt cgctccagga gcaaactgat gtcgtgagct taaactagtt cttgatgcag
     5101 atgacgtttt aagcacagaa gttaaaagag tgataacttc ttcagcttca aatatcaccc
     5161 cagctttttt ctgctcatga aggttagatg cctgctgctt aagtaattcc tctttatctg
     5221 taaaggcttt ttgaagtgca tcacctgacc gggcagatag ttcaccgggg tgagaaaaaa
     5281 gagcaacaac tgatttaggc aatttggcgg tgttgataca gcgggtaata atcttacgtg
     5341 aaatattttc cgcatcagcc agcgcagaaa tatttccagc aaattcattc tgcaatcggc
     5401 ttgcataacg ctgaccacgt tcataagcac ttgttgggcg ataatcgtta cccaatctgg
     5461 ataatgcagc catctgctca tcatccagct cgccaaccag aacacgataa tcactttcgg
     5521 taagtgcagc agctttacga cggcgactcc catcggcaat ttctatgaca ccagatactc
     5581 ttcgaccgaa cgccggtgtc tgttgaccag tcagtagaaa agaagggatg agatcatcca
     5641 gtgcgtcctc agtaagcagc tcctggtcac gttcattacc tgaccatacc cgagaggtct
     5701 tctcaacact atcaccccgg agcacttcaa gagtaaactt cacatcccga ccacatacag
     5761 gcaaagtaat ggcattaccg cgagccatta ctcctacgcg cgcaattaac gaatccacca
     5821 tcggggcagc tggtgtcgat aacgaagtat cttcaaccgg ttgagtattg agcgtatgtt
     5881 ttggaataac aggcgcacgc ttcattatct aatctcccag cgtggtttaa tcagacgatc
     5941 gaaaatttca ttgcagacag gttcccaaat agaaagagca tttctccagg caccagttga
     6001 agagcgttga tcaatggcct gttcaaaaac agttctcatc cggatctgac ctttaccaac
     6061 ttcatccgtt tcacgtacaa cattttttag aaccatgctt ccccaggcat cccgaatttg
     6121 ctcctccatc cacggggact gagagccatt actattgctg tatttggtaa gcaaaatacg
     6181 tacatcaggc tcgaaccctt taagatcaac gttcttgagc agatcacgaa gcatatcgaa
     6241 aaactgcagt gcggaggtgt agtcaaacaa ctcagcaggc gtgggaacaa tcagcacatc
     6301 agcagcacat acgacattaa tcgtgccgat acccaggtta ggcgcgctgt caataactat
     6361 gacatcatag tcatgagcaa cagtttcaat ggccagtcgg agcatcaggt gtggatcggt
     6421 gggcagttta ccttcatcaa atttgcccat taactcagtt tcaatacggt gcagagccag
     6481 acaggaagga ataatgtcaa gccccggcca gcaagtgggc tttattgcat aagtgacatc
     6541 gtccttttcc ccaagataga aaggcaggag agtgtcttct gcatgaatat gaagatctgg
     6601 tacccatccg tgatacattg aggctgttcc ctgggggtcg ttaccttcca cgagcaaaac
     6661 acgtagcccc ttcagagcca gatcctgagc aagatgaaca gaaactgagg ttttgtaaac
     6721 gccaccttta tgggcagcaa ccccgatcac cggtggaaat acgtcttcag cacgtcgcaa
     6781 tcgcgtacca aacacatcac gcatatgatt aatttgttca attgtataac caacacgttg
     6841 ctcaacccgt cctcgaattt ccatatccgg gtgcggtagt cgccctgctt tctcggcatc
     6901 tctgatagcc tgagaagaaa ccccaactaa atccgctgct tcacctattc tccagcgccg
     6961 ggttattttc ctcgcttccg ggctgtcatc attaaactgt gcaatggcga tagccttcgt
     7021 catttcatga ccagcgttta tgcactggtt aagtgtttcc atgagtttca ttctgaacat
     7081 cctttaatca ttgctttgcg tttttttatt aaatcttgca atttactgca aagcaacaac
     7141 aaaatcgcaa agtcatcaaa aaaccgcaaa gttgtttaaa ataagagcaa cactacaaaa
     7201 ggagataaga agagcacata cctcagtcac ttattatcac tagcgctcgc cgcagccgtg
     7261 taaccgagca tagcgagcga actggcgagg aagcaaagaa gaactgttct gtcagatagc
     7321 tcttacgctc agcgcaagaa gaaatatcca ccgtgggaaa aactccaggt agaggtacac
     7381 acgcggatag ccaattcaga gtaataaact gtgataatca accctcatca atgatgacga
     7441 actaaccccc gatatcaggt cacatgacga agggaaagag aaggaaatca actgtgacaa
     7501 actgccctca aatttggctt ccttaaaaat tacagttcaa aaagtatgag aaaatccatg
     7561 caggctgaag gaaacagcaa aactgtgaca aattaccctc agtaggtcag aacaaatgtg
     7621 acgaaccacc ctcaaatctg tgacagataa ccctcagact atcctgtcgt catggaagtg
     7681 atatcgcgga aggaaaatac gatatgagtc gtctggcggc ctttcttttt ctcaatgtat
     7741 gagaggcgca ttggagttct gctgttgatc tcattaacac agacctgcag gaagcggcgg
     7801 cggaagtcag gcatacgctg gtaactttga ggcagctggt aacgctctat gatccagtcg
     7861 attttcagag agacgatgcc tgagccatcc ggcttacgat actgacacag ggattcgtat
     7921 aaacgcatgg catacggatt ggtgatttct tttgtttcac taagccgaaa ctgcgtaaac
     7981 cggttctgta acccgataaa gaagggaatg agatatgggt tgatatgtac actgtaaagc
     8041 cctctggatg gactgtgcgc acgtttgata aaccaaggaa aagattcata gcctttttca
     8101 tcgccggcat cctcttcagg gcgataaaaa accacttcct tccccgcgaa actcttcaat
     8161 gcctgccgta tatccttact ggcttccgca gaggtcaatc cgaatatttc agcatattta
     8221 gcaacatgga tctcgcagat accgtcatgt tcctgtaggg tgccatcaga ttttctgatc
     8281 tggtcaacga acagatacag catacgtttt tgatcccggg agagactata tgccgcctca
     8341 gtgaggtcgt ttgactggac gattcgcggg ctatttttac gtttcttgtg attgataacc
     8401 gctgtttccg ccatgacaga tccatgtgaa gtgtgacaag tttttagatt gtcacactaa
     8461 ataaaaaaga gtcaataagc agggataact ttgtgaaaaa acagcttctt ctgagggcaa
     8521 tttgtcacag ggttaagggc aatttgtcac agacaggact gtcatttgag ggtgatttgt
     8581 cacactgaaa gggcaatttg tcacaacacc ttctctagaa ccagcatgga taaaggccta
     8641 caaggcgctc taaaaaagaa gatctaaaaa ctataaaaaa aataattata aaaatatccc
     8701 cgtggataag tggataaccc caagggaagt tttttcaggc atcgtgtgta agcagaatat
     8761 ataagtgctg ttccctggtg cttcctcgct cactcgagct acggggtctg acgctcagtg
     8821 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8881 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8941 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9001 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9061 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9121 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9181 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9241 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9301 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9361 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9421 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9481 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9541 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9601 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9661 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9721 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9781 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9841 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9901 aataggggtt ccgcgcacat ttccccgaaa agtgccacct ggcggccgcc tttcagcaaa
     9961 aaaccccgcg agacccccga agaggccccg cggggttatg ctaggtcgac ggagctcgaa
    10021 ttc
//
