LOCUS       sequence-311179-        2605 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the firefly luciferase reporter upon binding of a 
            transcriptional activator to target sites. The plasmid contains 10 
            copies of target sites for the TALEN1257 DNA-binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Mesko M, Lebar T, Dekleva P, Jerala R, Bencina M
  TITLE     Engineering and Rewiring of a Calcium-Dependent Signaling Pathway.
  JOURNAL   ACS Synth Biol. 2020 Aug 21;9(8):2055-2065. doi: 
            10.1021/acssynbio.0c00133. Epub 2020 Jul 20.
  PUBMED    32643923
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00133"; journalName: "ACS Synth Biol"; date: 
            "2020-08-21- 21"; volume: "9"; issue: "8"; pages: "2055-2065"
COMMENT     Sequence Label: 10TALE_Pmin_fLuc
FEATURES             Location/Qualifiers
     source          1..2605
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        706..737
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             776..2425
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             2432..2479
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             2483..2602
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
ORIGIN
        1 tttactgctg ctcccgcttc ttccgtttcc acatcttgct ggcagccggg gtaccgcagg
       61 caagtcgctt tactgctgct cccgcttctt ccgtttccac atctatggaa tcacggcggc
      121 cgcccgtatc tcatccttta ctgctgctcc cgcttcttcc gtttccacat ctaatgcgtc
      181 ggcgtgcggc cgctgccagt tgcatttact gctgctcccg cttcttccgt ttccacatct
      241 ttggctgtcc tagggtacct ggacgctcct tgtttactgc tgctcccgct tcttccgttt
      301 ccacatcttg gctggtgccg caccggtaag cagaactaga ggtggcaccg gtttgattgc
      361 acagctttac tgctgctccc gcttcttccg tttccacatc ttgctggcag ccggggtacc
      421 gcaggcaagt cgctttactg ctgctcccgc ttcttccgtt tccacatcta tggaatcacg
      481 gcggccgccc gtatctcatc ctttactgct gctcccgctt cttccgtttc cacatctaat
      541 gcgtcggcgt gcggccgctg ccagttgcat ttactgctgc tcccgcttct tccgtttcca
      601 catctttggc tgtcctaggg tacctggacg ctccttgttt actgctgctc ccgcttcttc
      661 cgtttccaca tcttggctgg tgccgcaccg gtaagcagag gatcttagag ggtatataat
      721 ggaagctcga cttccagctc gagggcaatc cggtactgtt ggtaaagcca ccatgatgga
      781 agatgccaaa aacattaaga agggcccagc gccattctac ccactcgaag acgggaccgc
      841 cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt
      901 taccgacgca catatcgagg tggacattac ctacgccgag tacttcgaga tgagcgttcg
      961 gctggcagaa gctatgaagc gctatgggct gaatacaaac catcggatcg tggtgtgcag
     1021 cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt
     1081 ggccccagct aacgacatct acaacgagcg cgagctgctg aacagcatgg gcatcagcca
     1141 gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa
     1201 gctaccgatc atacaaaaga tcatcatcat ggatagcaag accgactacc agggcttcca
     1261 aagcatgtac accttcgtga cttcccattt gccacccggc ttcaacgagt acgacttcgt
     1321 gcccgagagc ttcgaccggg acaaaaccat cgccctgatc atgaacagta gtggcagtac
     1381 cggattgccc aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc
     1441 ccgcgacccc atcttcggca accagatcat ccccgacacc gctatcctca gcgtggtgcc
     1501 atttcaccac ggcttcggca tgttcaccac gctgggctac ttgatctgcg gctttcgggt
     1561 cgtgctcatg taccgcttcg aggaggagct attcttgcgc agcttgcaag actataagat
     1621 tcaatctgcc ctgctggtgc ccacactatt tagcttcttc gctaagagca ctctcatcga
     1681 caagtacgac ctaagcaact tgcacgagat cgccagcggc ggggcgccgc tcagcaagga
     1741 ggtaggtgag gccgtggcca aacgcttcca cctaccaggc atccgccagg gctacggcct
     1801 gacagaaaca accagcgcca ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt
     1861 aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac ttggacaccg gtaagacact
     1921 gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga gcggctacgt
     1981 taacaacccc gaggctacaa acgctctcat cgacaaggac ggctggctgc acagcggcga
     2041 catcgcctac tgggacgagg acgagcactt cttcatcgtg gaccggctga agagcctgat
     2101 caaatacaag ggctaccagg tagccccagc cgaactggag agcatcctgc tgcaacaccc
     2161 caacatcttc gacgccgggg tcgccggcct gcccgacgac gatgccggcg agctgcccgc
     2221 cgcagtcgtc gtgctggaac acggtaaaac catgaccgag aaggagatcg tggactatgt
     2281 ggccagccag gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt
     2341 gcctaaagga ctgaccggca agttggacgc ccgcaagatc cgcgagattc tcattaaggc
     2401 caagaagggc ggcaagatcg ccgtgaattc tgcttgcaag aactggttca gtagcttaag
     2461 ccactttgtg atccacctta acagccacgg cttccctccc gaggtggagg agcaggccgc
     2521 cggcaccctg cccatgagct gcgcccagga gagcggcatg gatagacacc ctgctgcttg
     2581 cgccagcgcc aggatcaacg tctaa
//
