LOCUS       sequence-311195-        9002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  making transgenics expressing a QUAS driven YFP and NTR 2.0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Sharrock AV, Mulligan TS, Hall KR, Williams EM, White DT, Zhang L, 
            Emmerich K, Matthews F, Nimmagadda S, Washington S, Le KD, Meir-Levi
            D, Cox OL, Saxena MT, Calof AL, Lopez-Burks ME, Lander AD, Ding D, 
            Ji H, Ackerley DF, Mumm JS
  TITLE     NTR 2.0: a rationally engineered prodrug-converting enzyme with 
            substantially enhanced efficacy for targeted cell ablation.
  JOURNAL   Nat Methods. 2022 Feb;19(2):205-215. doi: 
            10.1038/s41592-021-01364-4. Epub 2022 Feb 7.
  PUBMED    35132245
REFERENCE   2  (bases 1 to 9002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-021-01364-4"; journalName: "Nat Methods"; date: 
            "2022-02"; volume: "19"; issue: "2"; pages: "205-215"
COMMENT     Sequence Label: 5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:ECFP
FEATURES             Location/Qualifiers
     source          1..9002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(82..103)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(452..463,464..626)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     999..1015
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1035..1055
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             1425..2141
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein"
                     /label=TagYFP
                     /note="mammalian codon optimized"
                     /translation="MVSKGEELFAGIVPVLIELDGDVHGHKFSVRGEGEGDADYGKLEI
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPKHMKMNDFFKSAMPEGYIQERTILFQDD
                     GKYKTRGEVKFEGDTLVNRIELKGKDFKEDGNILGHKLEYSFNSHNVYITPDKANNGLE
                     VNFKTRHNIEGGGVQLADHYQTNVPLGDGPVLIPINHYLSYQTDISKDRNEARDHMVLL
                     ESVSACSHTHGMDELYR"
     CDS             2163..2219
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     intron          2905..3477
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_signal    3554..3675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(4004..4037)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      4517..4526
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4523..4525,4526..4528,4529..5242)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     5176..5197
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5605..5622)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5611..5836
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(5837..5857)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(5865..5881)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5897..6031
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(6470..6657,6658..6669)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6703..6719)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6703..6719)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6756..6776)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6756..6774)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6795..6811)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6808..6830)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6819..6835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6843..6849,6850..6867,6868..6873))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6888..6909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7026..7043)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7197..7785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7277..7296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7956..8747,8748..8816))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8579..8598
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8817..8921)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
       61 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
      121 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccacgctg gctaagaact
      181 catcagcctc cccggtccat ctacccacgt accaatgcac caattggcca caatgacggc
      241 tactacatgg tgccattcct tcctctttat aggaatggag actacctcct gtccaacaat
      301 gctcttggat acgagtacgc ctacctgttg gacccaggtc attgcacaac accagaaatg
      361 ccctctgatc tgcaaaagac gtgaatatct gttcagacac ccatatccac tctgttccac
      421 acaggtcaga ggtttgtcca ggagttcttg acagaggtgt aaaaagtact caaaaatttt
      481 actcaagtga aagtacaagt acttagggaa aattttactc aattaaaagt aaaagtatct
      541 ggctagaatc ttacttgagt aaaagtaaaa aagtactcca ttaaaattgt acttgagtat
      601 taaggaagta aaagtaaaag caagaaagaa aactagagat tcttgtttaa gcttttaatc
      661 tcaaaaaaca ttaaatgaaa tgcatacaag gttttatcct gctttagaac tgtttgtatt
      721 taattatcaa actataagac agacaatcta atgccagtac acgctactca aagttgtaaa
      781 acctcagatt taacttcagt agaagctgat tctcaaaatt gttagtgtca agcctagctc
      841 ttttggggct gaaaagcaat cctgcagtgc tgaaaagcct ctcacaggca gccgatgcgg
      901 gaagaggtgt attagtcttg atagagaggc tgcaaatagc aggaaacgtg agcagagact
      961 ccctggtgtc tgaaacacag gccagatggg ccctcgagca ggaaacagct atgaccatga
     1021 ttacgccaag ctatcaactt tgtatagaaa agttggggta atcgcttatc ctcggataaa
     1081 caattatcct cacgggtaat cgcttatccg ctcgggtaat cgcttatcct cgggtaatcg
     1141 cttatccttg caagggtcga ctctagaggg tatataatgg atcccatcgc gtctcagcct
     1201 cactttgagc tcctccacac gaattccctc gacctcgaag acgcgtagca gggtagactg
     1261 tagagggtat ataatggaac ccatcgcgtc tcagcctcac tttgagctcc tccacacgat
     1321 ttcaccaggc cgccaccatg ctgtgttgta tcagaagaac aaagcctgtg gagaagaatg
     1381 aggaggctga ccaggaactg cagcctgcag gaagcacagc tacaatggtt agcaaaggcg
     1441 aggagctgtt cgccggcatc gtgcccgtgc tgatcgagct ggacggcgac gtgcacggcc
     1501 acaagttcag cgtgcgcggc gagggcgagg gcgacgccga ctacggcaag ctggagatca
     1561 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctggtg accaccctca
     1621 cctacggcgt acagtgcttc gcccgctacc ccaagcacat gaagatgaac gacttcttca
     1681 agagcgccat gcccgagggc tacatccagg agcgcaccat cctcttccaa gacgacggca
     1741 agtacaagac ccgcggcgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1801 tgaagggcaa ggacttcaag gaggacggca acatcctggg ccacaagctg gagtacagct
     1861 tcaacagcca caacgtctac atcacccccg acaaggccaa caacggcctg gaggtgaact
     1921 tcaagacccg ccacaacatc gagggcggcg gcgtgcagct ggccgaccac taccagacca
     1981 acgtgcccct gggcgacggc cccgtgctga tccccatcaa ccactacctg agctaccaga
     2041 ccgacatcag caaggaccgc aacgaggccc gcgaccacat ggtgctcctg gagtccgtca
     2101 gcgcctgcag ccacacccac ggcatggacg agctgtaccg ctacttgtac aagggaagcg
     2161 gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac cccgggccta
     2221 tgactattgt tcaagctgcc caatcccgct actccaccaa agcctttgat gcttcgcgca
     2281 aattgcctga agagaaagtc gcggcagtga aagagttaat ccgcatgagt gcgtccagtg
     2341 tcaactcgca accttggcat tttattgtcg cgagcagtga agagggaaaa gcgcgcatcg
     2401 ccaaagcaac acaaggtggt tttgctgcta atgagcgcaa gattttggat gcttctcacg
     2461 ttgtggtgtt ctgcgccaaa acggcgattg atgaagcgta cctactcgac cttttggaaa
     2521 gcgaagacaa agatggccgc tacgccgatg tcgaagcaaa aaatggcatg cacgctggtc
     2581 gttcattttt cgtcaacatg caccgctttg acttgaaaga cgcgcatcac tggatggaaa
     2641 agcaagttta cctcaatgtg gggacgctgc tattgggtgc ttctgcgatg gagatcgacg
     2701 cggtgccaat tgaaggcttc gatgccaagg tgcttgatga ggagtttggt ctgcgtgaga
     2761 agggctttac cagcgtggtg attgtgccgc tgggttacca tagcgaagac gattttaatg
     2821 ctaagctgcc aaaatcacgt tggtcagcag agactgtttt taccgaaatc taagtcgagc
     2881 acgaccgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc tttctttttc
     2941 gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt gtttagaatg
     3001 ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt tcactttcta
     3061 ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac tttttcgtta
     3121 aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt gtcagattgt
     3181 aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata ttgtacttca
     3241 gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt tctgcatata
     3301 aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacaccct ggtcatcatc
     3361 ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat aaaatactct
     3421 gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttctcttt cctacagctc
     3481 ctgggcaacg tgctggttgt tgtgctgtct catcattttg gcaaagaatt aattcctcga
     3541 cgtccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     3601 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     3661 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     3721 gtgtgggagg ttttttgtcg cgaatctaga ttaagattga atcctgttgc cggtcttgcg
     3781 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     3841 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     3901 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     3961 atgttactag atcgggaatt gatcccccct cgacagcaag cttataactt cgtatagcat
     4021 acattatacg aagttattca accactccag gcatagctag agccactgat gcatctatag
     4081 agctgaagta aagatagggt tgatgcaaat cgatttagaa ttttcaatgt agaaaaaaca
     4141 aaataaaaag tctattcact tgatggatag attagtcaaa caaacataga gtaggcactt
     4201 cccttgactc tgtaaacttt tggatttaac agattgtgca ctccttttag ttaaacacat
     4261 tagtccctga aaactaagac cctaaccata atcccattgt agacccacct actcgggtga
     4321 atataaagct cgcacagatc atctcacaga catcagacct tggcaaagga gcatcctgaa
     4381 catctctaca cactgaggca accctagggc cgccaccatg ctgtgttgta tcagaagaac
     4441 aaagcctgtg gagaagaatg aggaggctga ccaggaactg cagagcacgg taccgcgggc
     4501 ccgggatcca ccggccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     4561 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     4621 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     4681 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag
     4741 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     4801 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     4861 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     4921 ggacggcaac atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat
     4981 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     5041 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     5101 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     5161 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     5221 catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca
     5281 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     5341 aaaatgaatg caattgttgt tgttatcgaa ttctgcagga attgggtggc atccctgtga
     5401 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt
     5461 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg
     5521 gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta gggcacgagg
     5581 gggggcccga aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     5641 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     5701 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     5761 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaaggca tgctggggat
     5821 gcggtgggct ctatggcaac tttattatac atagttgata attcactggc cgtcgtttta
     5881 cggtaccatc gatgatgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     5941 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     6001 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     6061 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     6121 tatggctgat tatgatcctc tagatcagat ctgcgaagat acggccacgg gtgctcttga
     6181 tcctgtggct gattttggac tgtgctgctc gcagctgctg atgaatcaca tacttcctcc
     6241 attttcttcc actgattgac tgttataatt tccctaattt ccaggtcaag gtgctgtgca
     6301 ttgtggtaat agatgtgaca tgacgtcact tccaaaggac caatgaacat gtctgaccaa
     6361 tttcatataa tgtgaaaacg attttcatag gcagaataaa taacatttaa attaaactgg
     6421 gcatcagcgc aattcaattg gtttggtaat agcaagggaa aatagaatga agtgatctcc
     6481 aaaaaataag tactttttga ctgtaaataa aattgtaagg agtaaaaagt actttttttt
     6541 ctaaaaaaat gtaattaagt aaaagtaaaa gtattgattt ttaattgtac tcaagtaaag
     6601 taaaaatccc caaaaataat acttaagtac agtaatcaag taaaattact caagtacttt
     6661 acacctctgg ttcttgaccc cctaccttca gcaagcccag cagatccact agttctagag
     6721 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
     6781 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6841 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6901 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6961 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7021 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7081 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7141 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7201 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7261 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7321 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7381 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7441 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7501 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7561 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7621 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7681 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7741 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7801 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaaggcat tttggtcatg
     7861 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7921 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat aagtgaggca
     7981 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8041 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8101 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8161 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8221 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8281 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8341 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8401 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8461 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8521 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8581 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8641 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8701 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8761 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8821 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8881 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8941 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     9001 gc
//
