LOCUS       sequence-311200-       10846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi ftsI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S(RBSmut)_Psyn-sgRNAftsI
FEATURES             Location/Qualifiers
     source          1..10846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        572..606
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        628..703
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(2146..2160,2161..2166,2167..2183,2184..2190)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2166..2182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2198..2214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(2251..2277,2278..2280,2281..4767,4768..4770,
                     4771..6357)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6527..7318
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7441..8029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7930..7949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atttatagct ccttgaatac cggatttttt atctataaag tatatatatc acaattttga
       61 tttaaaagta aattcttttt ttaaaaatac aaaaattttt tataaattat atagaccatg
      121 atattgtatt aatactaatt gattttaatt taaggttgaa ctaaaataaa catagttttg
      181 tagaaaataa tttttcatta ttttgctact tgatattgga tcgtaaaaat attagggctt
      241 tacaaagttc ttttaaaaga gaatttagta aagccctaag tcttttaata aaaaattttt
      301 tgtaaaaaag ttggcaaaaa tagtttttgc tatatattta tatataagaa aaaattcaat
      361 actcaaggag ataaaaaatg gaaaacttaa acaataatcc acaaaaaatt aatcaaataa
      421 ataagcaaac taattttatt ggttttgaaa attttataga acttgataat caagttaatc
      481 aaactaaaga aaaaagtaaa ttaagtaaga taggtaagaa attacccgga ataagcagtc
      541 aagtctagtg cggccggaat tctaaagatc tttgacagct agctcagtcc taggtataat
      601 actagtgagt gttattaagc attctttgtt ttagagctag aaatagcaag ttaaaataag
      661 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt ggcgcgccaa
      721 ataatgcccg tgtccctgtt gaatagggac acggtttttt aacaattttt ttattcctag
      781 gccttagtgg ccggtacccg gggatcctta ttactggccg ctttctagcc tggatacttg
      841 tctggctagt tgcattagag agtcagctag agccctaggg ctagctgttt gtgtattcgg
      901 agccagtgta gtttttcttt ttactaacga tacgggtaat aattgattgc cttttacagc
      961 ctgaccttga gacaattgca aaagtctgtc cacagaggtt tgacctaata atctaaaatc
     1021 ttgtttgata gtagtaagtg gaggtatgta gcaactagaa tcttctgtat catcataacc
     1081 cacaactgaa atatcagccc caactctaag tccactttca gtaatagctc gcatagctcc
     1141 taaagccatt tgatcattag caactaacat agcagtaggg acaatgcctt catttaacat
     1201 ttgcattgtt tgctgaaatc ctgacatagc agaccaatct ccttctcttt ctgctattgg
     1261 ttgtatttga tttcttgtta aatacttgtg ccatcctgct aatctcaatc tggcacttac
     1321 tgaacttaag ggacctgcaa gtagtgcaat ttgttgatgg cctaatgcaa caaggtgttc
     1381 aactcctaac cttgttccat cttcgtgaga aaaaattata gagtttattg gggtttggtc
     1441 cgacacatcc aaaaacaatg ctggaacatt agtacatgct gcttcaactg caatcgcatc
     1501 ttggtcatca agaggataat taattataag tccactaaca cgttgagcta ataaattatg
     1561 tactgctgcc ttacaagcct ctacacctga tctctcaacc attgatacaa caacgcttgc
     1621 accaagttga tcagctctcg atttaattgc ggctacaatt tgactaggcg catgtaatgc
     1681 aagtgatgat gtggcaactc caattaaaag tgattgtttc cccgcaagtt gctgagcaac
     1741 gcgatttggt atataattca actctgccat agctgcttca accttctctc ttgtttttgc
     1801 agatacatga cttgcttggt tcacaactct cgaaaccgtt tggtagctaa ccccggcata
     1861 ttcagcaaca tcatacaaag taacaggttt catggatcat tcctccatga taaaatttaa
     1921 atttctgact ttggcaaatc cttttgcctg ttaaaaagaa tagcacaaaa attaaaaaaa
     1981 aagaaacgca aatagcaata gcattaaaaa taattattat tatttatttc tttgattaaa
     2041 atcaaaagcc tttaatgtat tttcttgatc tttcatgcgc ttaacggtaa aatccaaggt
     2101 attaagttca ccttccactt ttctctctct atcttctcta gaaaatcata aaaaatttat
     2161 ttgctttgtg agcggataac aattataata gattcaattg tgagcggata acaatttcac
     2221 acagaattca ttaaagaaga gaaattacat atggataaga aatactcaat aggcttagct
     2281 atcggcacaa atagcgtcgg atgggcggtg atcactgatg aatataaggt tccgtctaaa
     2341 aagttcaagg ttctgggaaa tacagaccgc cacagtatca aaaaaaatct tataggggct
     2401 cttttatttg acagtggaga gacagcggaa gcgactcgtc tcaaacggac agctcgtaga
     2461 aggtatacac gtcggaagaa tcgtatttgt tatctacagg agattttttc aaatgagatg
     2521 gcgaaagtag atgatagttt ctttcatcga cttgaagagt cttttttggt ggaagaagac
     2581 aagaagcatg aacgtcatcc tatttttgga aatatagtag atgaagttgc ttatcatgag
     2641 aaatatccaa ctatctatca tctgcgaaaa aaattggtag attctactga taaagcggat
     2701 ttgcgcttaa tctatttggc cttagcgcat atgattaagt ttcgtggtca ttttttgatt
     2761 gagggagatt taaatcctga taatagtgat gtggacaaac tatttatcca gttggtacaa
     2821 acctacaatc aattatttga agaaaaccct attaacgcaa gtggagtaga tgctaaagcg
     2881 attctttctg cacgattgag taaatcaaga cgattagaaa atctcattgc tcagctcccc
     2941 ggtgagaaga aaaatggctt atttgggaat ctcattgctt tgtcattggg tttgacccct
     3001 aattttaaat caaattttga tttggcagaa gatgctaaat tacagctttc aaaagatact
     3061 tacgatgatg atttagataa tttattggcg caaattggag atcaatatgc tgatttgttt
     3121 ttggcagcta agaatttatc agatgctatt ttactttcag atatcctaag agtaaatact
     3181 gaaataacta aggctcccct atcagcttca atgattaaac gctacgatga acatcatcaa
     3241 gacttgactc ttttaaaagc tttagttcga caacaacttc cagaaaagta taaagaaatc
     3301 ttttttgatc aatcaaaaaa cggatatgca ggttatattg atgggggagc tagccaagaa
     3361 gaattttata aatttatcaa accaatttta gaaaaaatgg atggtactga ggaattattg
     3421 gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa cggctctatt
     3481 ccccatcaaa ttcacttggg tgagctgcat gctattttga gaagacaaga agacttttat
     3541 ccatttttaa aagacaatcg tgagaagatt gaaaaaatct tgacttttcg aattccttat
     3601 tatgttggtc cattggcgcg tggcaatagt cgttttgcat ggatgactcg gaagtctgaa
     3661 gaaacaatta ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc agctcaatca
     3721 tttattgaac gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt actaccaaaa
     3781 catagtttgc tttatgagta ttttacggtt tataacgaat tgacaaaggt caaatatgtt
     3841 actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc cattgttgat
     3901 ttactcttca aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa
     3961 aaaatagaat gttttgatag tgttgaaatt tcaggagttg aagatagatt taatgcttca
     4021 ttaggtacct accatgattt gctaaaaatt attaaagata aagatttttt ggataatgaa
     4081 gaaaatgaag atatcttaga ggatattgtt ttaacattga ccttatttga agatagggag
     4141 atgattgagg aaagacttaa aacatatgct cacctctttg atgataaggt gatgaaacag
     4201 cttaaacgtc gccgttatac tggttgggga cgtttgtctc gaaaattgat taatggtatt
     4261 agggataagc aatctggcaa aacaatatta gattttttga aatcagatgg ttttgccaat
     4321 cgcaatttta tgcagctgat ccatgatgat agtttgacat ttaaagaaga cattcaaaaa
     4381 gcacaagtgt ctggacaagg cgatagttta catgaacata ttgcaaattt agctggtagc
     4441 cctgctatta aaaaaggtat tttacagact gtaaaagttg ttgatgaatt ggtcaaagta
     4501 atggggcggc ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact
     4561 caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa
     4621 ttaggaagtc agattcttaa agagcatcct gttgaaaata ctcaattgca aaatgaaaag
     4681 ctctatctct attatctcca aaatggaaga gacatgtatg tggaccaaga attagatatt
     4741 aatcgtttaa gtgattatga tgtcgatgcc attgttccac aaagtttcct taaagacgat
     4801 tcaatagaca ataaggtctt aacgcgttct gataaaaatc gtggtaaatc ggataacgtt
     4861 ccaagtgaag aagtagtcaa aaagatgaaa aactattgga gacaacttct aaacgccaag
     4921 ttaatcactc aacgtaagtt tgataattta acgaaagctg aacgtggagg tttgagtgaa
     4981 cttgataaag ctggttttat caaacgccaa ttggttgaaa ctcgccaaat cactaagcat
     5041 gtggcacaaa ttttggatag tcgcatgaat actaaatacg atgaaaatga taaacttatt
     5101 cgagaggtta aagtgattac cttaaaatct aaattagttt ctgacttccg aaaagatttc
     5161 caattctata aagtacgtga gattaacaat taccatcatg cccatgatgc gtatctaaat
     5221 gccgtcgttg gaactgcttt gattaagaaa tatccaaaac ttgaatcgga gtttgtctat
     5281 ggtgattata aagtttatga tgttcgtaaa atgattgcta agtctgagca agaaataggc
     5341 aaagcaaccg caaaatattt cttttactct aatatcatga acttcttcaa aacagaaatt
     5401 acacttgcaa atggagagat tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga
     5461 gaaattgtct gggataaagg gcgagatttt gccacagtgc gcaaagtatt gtccatgccc
     5521 caagtcaata ttgtcaagaa aacagaagtc cagacaggcg gattctccaa ggagtcaatt
     5581 ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa aagactggga tccaaaaaaa
     5641 tatggtggtt ttgatagtcc aacggtagct tattcagtcc tagtggttgc taaggtggaa
     5701 aaagggaaat cgaagaagtt aaaatccgtt aaagagttac tagggatcac aattctggaa
     5761 agaagttcct ttgaaaaaaa tccgattgac tttttagaag ctaaaggata taaggaagtt
     5821 aaaaaagact taatcattaa actacctaaa tatagtcttt ttgagttaga aaacggtcgt
     5881 aaacggatgc tggctagtgc cggagaatta caaaaaggaa atgagctggc tctgccaagc
     5941 aaatatgtga attttttata tttagctagt cattatgaaa agttgaaggg tagtccagaa
     6001 gataacgaac aaaaacaatt gtttgtggag cagcataagc attatttaga tgagattatt
     6061 gagcaaatca gtgaattttc taagcgtgtt attttagcag atgccaattt agataaagtt
     6121 cttagtgcat ataacaaaca tagagacaaa ccaatacgtg aacaagcaga aaatattatt
     6181 catttattta cgttgacgaa tcttggagct cccgctgctt ttaaatattt tgatacaaca
     6241 attgatcgta aacgatatac gtctacaaaa gaagttttag atgccactct tatccatcaa
     6301 tccatcactg gtctttatga aacacgcatt gatttgagtc agctaggagg tgactaataa
     6361 aagcttgatc agatctcagc ttttttttga agtgcctggc agtaagttga aaaaatttga
     6421 aataaacttg gactatgtta ataaaaaaat acaaattaac gaacaaaaag atttgaatta
     6481 ttatataatt tagttaaaag caattttaaa tgagggaggt ttccatatga gggaagcggt
     6541 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     6601 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     6661 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     6721 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     6781 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     6841 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     6901 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     6961 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     7021 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     7081 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     7141 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     7201 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     7261 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg
     7321 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcatga ccaaaatccc
     7381 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7441 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7501 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7561 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7621 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7681 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7741 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7801 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7861 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7921 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7981 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8041 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8101 actagatccc cggcaagttc atccttaggg ttttcatatc tcttaaaaag tcaatttctg
     8161 cttgattaat ttcttgtgga ttattgtttt tgcggttttt cattttttat ctccttgagt
     8221 attgaatttt ttcttatata taaatatata gcaaaaacta tttttgccaa cttttttaca
     8281 aaaaattttt tattaaaaca cttagggctt tgctaaattc tcttttaaaa gaacttagta
     8341 aagccctatg gatttaagaa ctgattatac ttacgtagta aaaatactac agatattaat
     8401 agtatagacc atatcaaaat cataatcaat ttatatttat ctaaaattgt agtccataca
     8461 aaatacaaaa aaattaggtt tttctaaatt ctattttaaa agaacttagt aacacctaat
     8521 ctctaaatta cttacaaaat tctaaaatta gatttttagt tcttcatatt tctttaaaag
     8581 ttttttaaga aaatcttttt gattttcaaa aatttcatgc atcataaaac tagtaaattt
     8641 ggattttttt ttgtaaaaat cataactttc ttgggtttta agttgaaatc ttaatggttt
     8701 tattggattt tgcctagatt tttttaacgc cggactttct ttatctttta taaaattata
     8761 agaattttta ataccatttt ctattaaata atcttctttt ataagcccct cttgcagtgc
     8821 agttgctaat cttaaatact tatacgcttg agtttttgct acatcaaagt cttttataaa
     8881 atcaagaaaa gttttataac catcaaattt ataatattga ttatctttta tttcttttaa
     8941 aattttaata ttttccaccc tgtggaatat ttcttttttg aaattgtcat ttaacttttc
     9001 ctttagtgtc ttgtaataag aaattttact ttcattaatc attaaatttt gttcaacata
     9061 agtatctatc ttttttgtta aaactttaac aacacgtttt ttttccataa aaattttcct
     9121 ttatttttgc aacattttcc ttaattgata taattgttac tattttagat tttttaatat
     9181 ccacttaaga attaatccct cactagaata ttttttatta tagaattcat atacatgttt
     9241 ttctaaatct ataaatttat caacaattgc tttattatag ggtgtatcta tcttttcttt
     9301 tcttaataat cgtctcatat tctttaaata acaacaaaca ctgccggttt taaacctaaa
     9361 ttctatgtaa tatatttttg aaaaagtata tacattttca accccgttta tttcatactt
     9421 tacaaaaaca ttatttattg gttttctata accatagtaa atgcctaaaa atttgtcccc
     9481 gtctttcata ggatatagat aaagtttttg aatctctgat ttatctaata atttttcaaa
     9541 aaaaattata caccgctttt gtttgttact aactactcta tgcaatgcct ttaatatttt
     9601 tgtgtgatat atatttttat cacctttttt ttctttcagc aggaagatac tttcttttgg
     9661 aaatttaatt ttttgttcat ttattcttgc agccttaagt ccgattttca tatttaatcc
     9721 ttatatagcc tttctattta attcttttaa actcaataaa cttttttggt ttttcattag
     9781 ttctaataat tcgcaataat atgtgcaaaa cactttattg tattctattt tcttttggtt
     9841 attcaaatat tcttttataa ttggctttag aagttcaata tttacttctt tttttagttg
     9901 ttcaataagg atattgaaaa tgtttgtttt taaatcttga taattttgtt gagaattttc
     9961 tttttttctt tcaattgact tctctaattt acgctttata ttgtttaaat cgctatattt
    10021 atgactttca ataataaaat gaggcttaaa tttgtaattt tcgtatattt tttggaaact
    10081 tgtttctaat tgtttagaat tatattcatt tttttcaaat tctttttgag tgttgcatag
    10141 aatttccttt aatttttctt gtttttcttt catacaagat ttagtaaaat gtatattttt
    10201 attttttagt aatttaattt ctattatttt tatgatttta agtattttga ttatattatc
    10261 tttatcaata tttaaattga ataaaattgt aagaaaaatt gaaagaattt ttttacataa
    10321 aaagttacat ttattgaagt aatttcttac ttgatacttt tctatttcat taatctttct
    10381 ttcttctttt atattatttt tattatttaa acactccttt aaatctacac tcccattttt
    10441 agtaaatcta tctttaaggc cccttgaaac tctattttga aatcttaaat cttttttttc
    10501 ttgaaagtat ttatagattt tttggtaaca ttcttttttt gaaaaattaa gttgatagta
    10561 tatttcggtc cccaaattta cccctaagtg tttgtgataa ttagttgtga ctttaaattc
    10621 tttttctaat ttgtaaaggt aattttgcag tgtttttagt ttaacgggtg tttgactatt
    10681 tcttttaagg ttttcattaa agtaatagag tatgtttttt tgtgtgtatt tcttatatgt
    10741 tttatttatg tacgctagtg ttgagataag gactactaat ttatactgta atttattgta
    10801 gcaagttgtg gttttactgg ttttgtttat attagataag ttttcc
//
