LOCUS       sequence-311214-       17767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, 
            CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17767)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 17767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pRN120
FEATURES             Location/Qualifiers
     source          1..17767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1843..2640)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1954..1973)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2564..2583
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2703..3380)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    3571..3592
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3607..3612,3613..3630,3631..3637)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3645..3661
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3650..3672
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3669..3685
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3669..3685
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4596..6248
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4662..4680)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      6258..6506
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        6652..6831
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        6684..6867
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     polyA_signal    11124..11298
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12999..13023
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             14323..14952
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             15386..16453
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      16519..16713
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(16949..16971)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    17057..17197
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     17112..17131
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(17212..17229)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(17383..17767,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17463..17482)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgactctag ctagaggatc gatccgaacc ccagagtccc gctcagaaga actcgtcaag
     1861 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     1921 gcggtcagcc cattcgccgc caagttcttc agcaatatca cgggtagcca acgctatgtc
     1981 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     2041 ttccaccatg atattcggca agcaggcatc gccatgtgtc acgacgagat cctcgccgtc
     2101 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgttcttc
     2161 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     2221 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     2281 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     2341 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     2401 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctggag
     2461 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     2521 cagccgaaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     2581 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatccccat
     2641 ggtcgatcga cagatctgcg aaagctcgag agagatagat ttgtagagag agactggtga
     2701 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg tcttgcgaag
     2761 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct
     2821 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
     2881 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat
     2941 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg
     3001 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc
     3061 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca
     3121 ccatgttatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
     3181 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
     3241 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
     3301 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
     3361 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
     3421 tggagtagac gagagtgtcg tgctccacca tgttggcaag ctgctctagc caatacgcaa
     3481 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     3541 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     3601 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     3661 atttcacaca ggaaacagct atgaccatga ttacgaattc gagctcaaag tttaacgcgt
     3721 tagcagaagg catgttgttg tgactccgag gggttgcctc aaactctatc ttataaccgg
     3781 cgtggaggca tggaggcagg ggtattttgg tcattttaat agatagtgga aaatgacgtg
     3841 gaatttactt aaagacgaag tctttgcgac aagggggggc ccacgccgaa tttaatatta
     3901 ccggcgtggc ccccccttat cgcgagtgct ttagcacgag cggtccagat ttaaagtaga
     3961 aaatttcccg cccactaggg ttaaaggtgt tcacactata aaagcatata cgatgtgatg
     4021 gtatttgatg gagcgtatat tgtatcaggt atttccgttg gatacgaatt attcgtacga
     4081 ccctcggtac cgatcaaaag caggtgtcat gatcggcgcg cctggcagac atactgtccc
     4141 acaaatgaag atggaatctg taaaagaaaa cgcgtgaaat aatgcgtctg acaaaggtta
     4201 ggtcggctgc ctttaatcaa taccaaagtg gtccctacca cgatggaaaa actgtgcagt
     4261 cggtttggct ttttctgacg aacaaataag attcgtggcc gacaggtggg ggtccaccat
     4321 gtgaaggcat cttcagactc caataatgga gcaatgacgt aagggcttac gaaataagta
     4381 agggtagttt gggaaatgtc cactcacccg tcagtctata aatacttagc ccctccctca
     4441 ttgttaaggg agcaaaatct cagagagata gtcctagaga gagaaagaga gcaagtagcc
     4501 tagaagtagt caaggcggcg aagtattcag gcacgtggcc aggaagaaga aaagccaaga
     4561 cgacgaaaac aggtaagagc taagcttcct gcaggatgga agacgccaaa aacataaaga
     4621 aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg
     4681 ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg
     4741 tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac
     4801 gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct
     4861 ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt
     4921 ataatgaacg tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt
     4981 ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa
     5041 ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca
     5101 catctcatct acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg
     5161 acaagacaat tgcactgatc atgaactcct ctggatctac tggtctgcct aaaggtgtcg
     5221 ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca
     5281 atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa
     5341 tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg
     5401 aagaagagct gtttctgagg agccttcagg attacaagat tcaaagtgcg ctgctggtgc
     5461 caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat ttatctaatt
     5521 tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa gcggttgcca
     5581 agaggttcca tctgccaggt atcaggcaag gatatgggct cactgagact acatcagcta
     5641 ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt
     5701 ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat caaagaggcg
     5761 aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg gaagcgacca
     5821 acgccttgat tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag
     5881 acgaacactt cttcatcgtt gaccgcctga agtctctgat taagtacaaa ggctatcagg
     5941 tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc gacgctggtg
     6001 tcgcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc
     6061 acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg
     6121 cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa
     6181 aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagatcg
     6241 ccgtgtgacg tcgacgatat gaagatgaag atgaaatatt tggtgtgtca aataaaaagc
     6301 ttgtgtgctt aagtttgtgt ttttttcttg gcttgttgtg ttatgaattt gtggcttttt
     6361 ctaatattaa atgaatgtaa gatcacatta taatgaataa acaaatgttt ctataatcca
     6421 ttgtgaatgt tttgttggat ctcttctgca gcatataact actgtatgtg ctatggtatg
     6481 gactatggaa tatgattaaa gataagccag agctctggtg acggacggcg cgactagttt
     6541 tacgtacgtt aattaacccg ggcgcgccga tcatgagcgg agaattaagg gagtcacgtt
     6601 atgacccccg ccgatgacgc gggacaagcc gttttacgtt tggaactgac agaaccgcaa
     6661 cgttgaagga gccactcagc cgcgggtttc tggagtttaa tgagctaagc acatacgtca
     6721 gaaaccatta ttgcgcgttc aaaagtcgcc taaggtcact atcagctagc aaatatttct
     6781 tgtcaaaaat gctccactga cgttccataa attcccctcg gtatccaatt agagtctcat
     6841 attcactctc aatccaaata atctgcaccg tacctgcagg gtccgagcta ggtcacagaa
     6901 gcgctcagga aggccgctga gatagaggca tggcggccaa tgcgggcggc ggtggagcgg
     6961 gaggaggcag cggcagcggc agcgtggctg cgccggcggt gtgccgcccc agcggctcgc
     7021 ggtggacgcc gacgccggag cagatcagga tgctgaagga gctgtactac ggctgcggca
     7081 tccggtcgcc cagctcggag cagatccagc gcatcaccgc catgctgcgg cagcacggca
     7141 agatcgaggg caagaacgtc ttctactggt tccagaacca caaggcccgc gagcgccaga
     7201 agcgccgcct caccagcctc gacgtgaacg tgcccgccgc cggcgcggcc gacgccacca
     7261 ccagccaact cggcgtcctc tcgctgtcgt cgccgccgcc ttcaggcgcg gcgcctccct
     7321 cgcccaccct cggcttctac gccgccggca atggcggcgg atcggctgtg ctgctggaca
     7381 cgagttccga ctggggcagc agcggcgctg cgatggccac cgagacatgc ttcctccagg
     7441 actacatggg cgtgacggac acgggcagct cgtcgcagtg gccacgcttc tcgtcgtcgg
     7501 acacgataat ggcggcggcc gcggcgcggg cggcgacgac gcgggcgccc gagactctcc
     7561 ctctcttccc gacctgcggc gacgacggcg gcagcggtag cagcagctac ttgccgttct
     7621 ggggtgccgc gtccacaact gccggcgcca cttcttccgt tgcgatccag cagcaacacc
     7681 agctgcagga gcagtacagc ttttacagca acagcaacag cacccagctg gccggcaccg
     7741 gcaaccaaga cgtatcggca acagcagcag cagccgccgc cctggagctg agcctcagct
     7801 catggtgctc cccttaccct gctgcaggga gtatgtgaga gcaacgcgag ctgccactgc
     7861 tcttcactta tgtctctgga atggaaggag gaggaagtga gcatagcgtt ggtgcgttgc
     7921 tgtcattgtc ctaggttagt agctagtgcc agttactagt aagcatcagg cataggagta
     7981 tgtagtagaa gcatgcacgt tgccggccag ccaggcttta gacgggaaaa gaatttggtg
     8041 cagccggctg caaaacagga tgtttacagc ccccccctcg agccctagac ttgtccatct
     8101 tctggattgg ccaagttaat taatgtatga aataaaagga tgcacacata gtgacatgct
     8161 aatcactata atgtgggcat caaagttgtg tgttatgtgt aattactaat tatctgaata
     8221 agagaaagag atcatccata tttcttatcc taaatgaatg tcacgtgtct ttataattct
     8281 ttgatgaacc agatgcattt tattaaccaa ttccatatac atataaatat taatcatata
     8341 taattaatat caattgggtt agcaaaacaa atctagtcta ggtgtgtttt gctaattatt
     8401 gggggatagt gcaaaaagaa atctacgttc tcaataattc agatagaaaa cttaataaag
     8461 tgagataatt tacatagatt gcttttatcc tttgatatat gtgaaaccat gcatgatata
     8521 aggaaaatag atagagaaat aattttttac atcgttgaat atgtaaacaa tttaattcaa
     8581 gaagctagga atataaatat tgaggagttt atgattagag ctctcccact aaacgtcccg
     8641 cgtcgagctg caggtcaacg gatcaggata ttcttgttta agatgttgaa ctctatggag
     8701 gtttgtatga actgatgatc taggaccgga taagttccct tcttcatagc gaacttattc
     8761 aaagaatgtt ttgtgtatca ttcttgttac attgttatta atgaaaaaat attattggtc
     8821 attggactga acacgagtgt taaatatgga ccaggcccca aataagatcc attgatatat
     8881 gaattaaata acaagaataa atcgagtcac caaaccactt gcctttttta acgagacttg
     8941 ttcaccaact tgatacaaaa gtcattatcc tatgcaaatc aataatcata caaaaatatc
     9001 caataacact aaaaaattaa aagaaatgga taatttcaca atatgttata cgataaagaa
     9061 gttacttttc caagaaattc actgatttta taagcccact tgcattagat aaatggcaaa
     9121 aaaaaacaaa aaggaaaaga aataaagcac gaagaattct agaaaatacg aaatacgctt
     9181 caatgcagtg ggacccacgg ttcaattatt gccaattttc agctccaccg tatatttaaa
     9241 aaataaaacg ataatgctaa aaaaatataa atcgtaacga tcgttaaatc tcaacggctg
     9301 gatcttatga cgaccgttag aaattgtggt tgtcgacgag tcagtaataa acggcgtcaa
     9361 agtggttgca gccggcacac acgagtcgtg tttatcaact caaagcacaa atacttttcc
     9421 tcaacctaaa aataaggcaa ttagccaaaa acaactttgc gtgtaaacaa cgctcaatac
     9481 acgtgtcatt ttattattag ctattgcttc accgccttag ctttctcgtg acctagtcgt
     9541 cctcgtcttt tcttcttctt cttctataaa acaataccca aagagctctt cttcttcaca
     9601 attcagattt caatttctca aaatcttaaa aactttctct caattctctc taccgtgatc
     9661 aaggtaaatt tctgtgttcc ttattctctc aaaatcttcg attttgtttt cgttcgatcc
     9721 caatttcgta tatgttcttt ggtttagatt ctgttaatct tagatcgaac acgattttct
     9781 gggtttgatc gttagatatc atcttaattc tcgattaggg tttcatagat atcatccgat
     9841 ttgttcaaat aatttgagtt ttgtcgaata attactcttc gatttgtgat ttctatctag
     9901 atctggtgtt agtttctagt ttgtgcgatc gaatttgtcg attaatctga gtttttctga
     9961 ttaacaggat ggagagtggt tccaacagca cttcttgtcc aatggctttt gccggggata
    10021 atagtgatgg tccgatgtgt cctatgatga tgatgatgcc gcccatcatg acatcacatc
    10081 aacatcatgg tcatgatcat caacatcaac aacaagaaca tgatggttat gcatatcagt
    10141 cacaccacca acaaagtagt tccctttttc ttcaatcact agctcctccc caaggaacta
    10201 agaacaaagt tgcttcttct tcttctcctt cctcttgtgc tcctgcctat tctctaatgg
    10261 agatccatca taacgaaatc gttgcaggag gaatcaaccc ttgctcctct tcctcttctt
    10321 cagcctctgt caaggccaag atcatggctc atcctcacta ccaccgcctc ttggccgctt
    10381 atgtcaattg tcagaaggtt ggagcaccac cggaggttgt ggcgaggcta gaggaggcat
    10441 gctcgtctgc cgcagccgct gccgcatcta tgggaccaac aggatgtcta ggtgaagatc
    10501 cagggcttga tcaattcatg gaagcttact gtgaaatgct cgttaagtat gagcaagagc
    10561 tctccaaacc tttcaaggaa gctatggtct tccttcaacg tgtcgagtgt caattcaaat
    10621 ccctctctct atcctcacct tcctctttct ccggttatgg agagacagca attgatagga
    10681 acaataatgg gtcatccgag gaagaagtcg atatgaacaa tgaatttgta gatccacaag
    10741 ctgaggatag agagcttaaa ggacagctct tgcgcaagta cagtggttac ttagggagcc
    10801 tcaagcaaga gttcatgaag aagaggaaga aaggaaagct ccctaaagaa gctcgtcaac
    10861 aactgcttga ttggtggagc cgtcactaca aatggcctta cccttcggag caacaaaagc
    10921 tcgcccttgc ggaatcaacg gggctggacc agaaacagat aaacaattgg ttcataaacc
    10981 agaggaaacg gcattggaag ccgtcggagg acatgcagtt tgtagtaatg gacgcaacac
    11041 atcctcacca ttacttcatg gataatgtct tgggcaatcc tttcccaatg gatcacatct
    11101 cctccaccat gctttgactc gagtttctcc ataataatgt gtgagtagtt cccagataag
    11161 ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
    11221 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
    11281 agtactaaaa tccagatccc ccgaattaag tgactacacc tgcaaaaagt gtttgatcgc
    11341 cggcggtacc gagtgtactt caagtcagtg ggaaatcaat aaaatgatta ttttatgaat
    11401 atatttcatt gtgcaagtag atagaaatta catatgttac ataacacacg aaataaacaa
    11461 aaaaagacaa tccaaaaaca aacaccccaa aaaaaataat cactttagat aaactcgtat
    11521 gaggagaggc acgttcagtg actcgacgat tcccgagcaa aaaaagtctc cccgtcacac
    11581 atgtagtggg tgacgcaatt atctttaaag taatccttct gttgacttgt cattgataac
    11641 atccagtctt cgtcaggatt gcaaagaatt atagaaggga tcccaccttt tattttcttc
    11701 ttttttccat atttagggtt gacagtgaaa tcagactggc aacctattaa ttgcttccac
    11761 aatgggacga acttgaaggg gatgtcgtcg atgatattat aggtggcgtg ttcatcgtag
    11821 ttggtgaaat cgatggtacc gttccaatag ttgtgtcgtc cgagacttct agcccaggtg
    11881 gtctttccgg tacgagttgg tccgcagatg tagaggctgg ggtgtcggat tccattcctt
    11941 ccattgtcct tgttaaatcg gccatccatt caaggtcaga ttgagcttgt tggtatgaga
    12001 caggatgtat gtaagtataa gcgtctatgc ttacatggta tagatgggtt tccctccagg
    12061 agtgtagatc ttcgtggcag cgaagatctg attctgtgaa gggcgacaca tacggttcag
    12121 gttgtggagg gaataatttg ttggctgaat attccagcca ttgaagcttt gttgcccatt
    12181 catgagggaa ttcttccttg atcatgtcaa gatattcctc cttagacgtt gcagtctgga
    12241 taatagttct ccatcgtgcg tcagatttgc gaggagaaac cttatgatct cggaaatctc
    12301 ctctggtttt aatatctccg tcctttgata tgtaatcaag gacttgttta gagtttctag
    12361 ctggctggat attagggtga tttccttcaa aatcgaaaaa agaaggatcc ctaatacaag
    12421 gttttttatc aagctggaga agagcatgat agtgggtagt gccatcttga tgaagctcag
    12481 aagcaacacc aaggaagaaa ataagaaaag gtgtgagttt ctcccagaga aactggaata
    12541 aatcatctct ttgagatgag cacttgggat aggtaaggaa aacatattta gattggagtc
    12601 tgaagttctt actagcagaa ggcatgttgt tgtgactccg aggggttgcc tcaaactcta
    12661 tcttataacc ggcgtggagg catggaggca ggggtatttt ggtcatttta atagatagtg
    12721 gaaaatgacg tggaatttac ttaaagacga agtctttgcg acaagggggg gcccacgccg
    12781 aatttaatat taccggcgtg gccccccctt atcgcgagtg ctttagcacg agcggtccag
    12841 atttaaagta gaaaatttcc cgcccactag ggttaaaggt gttcacacta taaaagcata
    12901 tacgatgtga tggtatttga tggagcgtat attgtatcag gtatttccgt tggatacgaa
    12961 ttattcgtac gaccctcata gtttaaacta tcagtgtttg acaggatata ttggcgggta
    13021 aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg tgaaaaggtt
    13081 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta
    13141 ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt
    13201 cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct
    13261 tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga
    13321 accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg
    13381 tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca
    13441 ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga
    13501 tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc
    13561 gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc
    13621 gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg
    13681 tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc
    13741 gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac
    13801 agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg
    13861 cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga
    13921 cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg
    13981 ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg
    14041 ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta
    14101 cgtgttcgag ccgcccgcgc acggctcaac cgtgcggctg catgaaatcc tggccggttt
    14161 gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg
    14221 ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta
    14281 tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta
    14341 cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg
    14401 caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat
    14461 tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt
    14521 gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag
    14581 gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag
    14641 ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt
    14701 gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg
    14761 cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc
    14821 cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa
    14881 tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca
    14941 aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg
    15001 ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc
    15061 cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt
    15121 acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac
    15181 cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc
    15241 atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg
    15301 ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc
    15361 cccaagcccg aggaatcggc gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc
    15421 ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg
    15481 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa
    15541 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga
    15601 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag
    15661 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat
    15721 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag
    15781 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg
    15841 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt
    15901 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg
    15961 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg
    16021 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga
    16081 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac
    16141 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg
    16201 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg
    16261 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt
    16321 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc
    16381 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc
    16441 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg
    16501 tcgaaaaggc ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg
    16561 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta
    16621 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat
    16681 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagcccaaga
    16741 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg
    16801 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg
    16861 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc
    16921 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gaaacggtca
    16981 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
    17041 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
    17101 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
    17161 accgcacaga tgcgtaagga gaaaataccg catcaggccc tcttccgctt cctcgctcac
    17221 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    17281 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    17341 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    17401 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    17461 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    17521 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    17581 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    17641 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    17701 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    17761 gaggtat
//
