LOCUS       sequence-311225-       10088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression of human TMPRSS2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Chavez Lab
  TITLE     Coronavirus host receptors and proteases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX307-TMPRSS2-G418
FEATURES             Location/Qualifiers
     source          1..10088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2804..2828)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2836..2877
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2928..3516
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2981..3001)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3399..3410)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3588..3821
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3899..4020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3936..3955)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3990..4009
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4060..4195
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4215..4234)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4216..4234)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4244..4261)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4244..4260)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4253..4275)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4402..4857
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4489..4508)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4699..4720
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4883..4987
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4988..5056,5057..5848)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5206..5225)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6019..6607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6508..6527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6761..6778
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6895..6916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6931..6936,6937..6954,6955..6961)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6969..6985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6974..6996
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6993..7009
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6993..7009
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7028..7048
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7030..7048
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7076..7302
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7303..7483
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7530..7655
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8148..8381
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8566..8610
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8841..9051
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8863..9059
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8910..9045
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9087..9881
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9087..9881
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9141..9160)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9751..9770
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    9929..10046
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctac
     1321 aaaatggctt tgaactcagg gtcaccacca gctattggac cttactatga aaaccatgga
     1381 taccaaccgg aaaaccccta tcccgcacag cccactgtgg tccccactgt ctacgaggtg
     1441 catccggctc agtactaccc gtcccccgtg ccccagtacg ccccgagggt cctgacgcag
     1501 gcttccaacc ccgtcgtctg cacgcagccc aaatccccat ccgggacagt gtgcacctca
     1561 aagactaaga aagcactgtg catcaccttg accctgggga ccttcctcgt gggagctgcg
     1621 ctggccgctg gcctactctg gaagttcatg ggcagcaagt gctccaactc tgggatagag
     1681 tgcgactcct caggtacctg catcaacccc tctaactggt gtgatggcgt gtcacactgc
     1741 cccggcgggg aggacgagaa tcggtgtgtt cgcctctacg gaccaaactt catccttcag
     1801 gtgtactcat ctcagaggaa gtcctggcac cctgtgtgcc aagacgactg gaacgagaac
     1861 tacgggcggg cggcctgcag ggacatgggc tataagaata atttttactc tagccaagga
     1921 atagtggatg acagcggatc caccagcttt atgaaactga acacaagtgc cggcaatgtc
     1981 gatatctata aaaaactgta ccacagtgat gcctgttctt caaaagcagt ggtttcttta
     2041 cgctgtatag cctgcggggt caacttgaac tcaagccgcc agagcaggat cgtgggcggc
     2101 gagagcgcgc tcccgggggc ctggccctgg caggtcagcc tgcacgtcca gaacgtccac
     2161 gtgtgcggag gctccatcat cacccccgag tggatcgtga cagccgccca ctgcgtggaa
     2221 aaacctctta acaatccatg gcattggacg gcatttgcgg ggattttgag acaatctttc
     2281 atgttctatg gagccggata ccaagtagaa aaagtgattt ctcatccaaa ttatgactcc
     2341 aagaccaaga acaatgacat tgcgctgatg aagctgcaga agcctctgac tttcaacgac
     2401 ctagtgaaac cagtgtgtct gcccaaccca ggcatgatgc tgcagccaga acagctctgc
     2461 tggatttccg ggtggggggc caccgaggag aaagggaaga cctcagaagt gctgaacgct
     2521 gccaaggtgc ttctcattga gacacagaga tgcaacagca gatatgtcta tgacaacctg
     2581 atcacaccag ccatgatctg tgccggcttc ctgcagggga acgtcgattc ttgccagggt
     2641 gacagtggag ggcctctggt cacttcgaag aacaatatct ggtggctgat aggggataca
     2701 agctggggtt ctggctgtgc caaagcttac agaccaggag tgtacgggaa tgtgatggta
     2761 ttcacggact ggatttatcg acaaatgagg gcagacggct aaaacccagc tttcttgtac
     2821 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     2881 taatgaacta gtaccggtta agtcgacaat caacgcgtta agtcgacaat caacctctgg
     2941 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     3001 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     3061 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     3121 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     3181 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     3241 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     3301 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     3361 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     3421 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     3481 agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta
     3541 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     3601 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     3661 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     3721 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     3781 atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg
     3841 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     3901 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3961 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4021 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     4081 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     4141 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     4201 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     4261 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     4321 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     4381 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4441 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4501 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4561 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4621 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4681 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4741 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4801 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     4861 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4921 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4981 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5041 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5101 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5161 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5221 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5281 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5341 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5401 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5461 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5521 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     5581 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     5641 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5701 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5761 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5821 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     5881 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     5941 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6001 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6061 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6121 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     6181 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6241 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6301 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6361 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6421 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6481 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6541 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6601 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6661 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     6721 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     6781 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     6841 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     6901 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     6961 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     7021 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     7081 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     7141 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     7201 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     7261 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     7321 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7381 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7441 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     7501 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     7561 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     7621 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     7681 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     7741 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     7801 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     7861 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     7921 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     7981 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     8041 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     8101 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     8161 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     8221 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     8281 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     8341 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     8401 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     8461 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     8521 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     8581 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     8641 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     8701 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     8761 cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca accaggtgtg
     8821 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc aattagtcag
     8881 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     8941 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     9001 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     9061 agctttctag aggtaccgtg gccaccatga ttgaacaaga tggattgcac gcaggttctc
     9121 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     9181 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     9241 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     9301 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     9361 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     9421 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     9481 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     9541 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     9601 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     9661 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     9721 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     9781 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     9841 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agttaacgaa ttctagatct
     9901 tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     9961 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
    10021 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
    10081 ggcgccgg
//
