LOCUS       sequence-311257-        2454 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Signaling reporter island (SiRI) construct for spatially multiplexed
            imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC 
            indicator), OLLAS tag, and S4 protein scaffold..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Linghu C, Johnson SL, Valdes PA, Shemesh OA, Park WM, Park D, 
            Piatkevich KD, Wassie AT, Liu Y, An B, Barnes SA, Celiker OT, Yao 
            CC, Yu CJ, Wang R, Adamala KP, Bear MF, Keating AE, Boyden ES
  TITLE     Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling 
            Network Dynamics.
  JOURNAL   Cell. 2020 Nov 17. pii: S0092-8674(20)31399-4. doi: 
            10.1016/j.cell.2020.10.035.
  PUBMED    33232692
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Nov 17. pii: S0092-8674(20)31399-4. doi: 
            10.1016/j.cell.2020.10.035."
COMMENT     Sequence Label: pAAV-UBC-S4-ExRaiCKAR
FEATURES             Location/Qualifiers
     source          1..2454
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1711..1752
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
ORIGIN
        1 atgattactg tgttcggact caagagcaaa ctggcaccaa gaagagagaa gctcgcagag
       61 gttatctaca gcagcctgca cctcggactg gacattccca agggaaagca cgctatccgc
      121 tttctgtgtc tggagaagga ggacttctac tatcctttcg atcgctccga cgactacact
      181 gtgattgaga tcaacctcat ggctggacgc agcgaagaga ctaagatgct cctcatcttt
      241 ctgctgttca ttgcactcga acgcaagctg ggaatcaggg ctcatgacgt tgagatcacc
      301 atcaaggagc agccagctca ttgctgggga ttcagaggac gcactggaga tagcgcacgc
      361 gacctggatt acgatatcta tgtgggaggt tctggaggtt ctggtggtac tggtggttct
      421 ggaggatctg gtggtacagg aggtagcgga ggttctggtg gaaccggagg tagattcaga
      481 cgctttagga ctctcaaaga caaggctaag gctggaggta ccggaggatc tgagctgtct
      541 gcaatcaatg tgtacatcaa agctgataag cagaagaacg gcatcaaggc taacttcaag
      601 attcgccaca acattgagga cggaggcgtc cagctcgctt accactacca gcagaacaca
      661 cctattggcg acggtccagt tctcctgcct gacaatcact acctgtctgt gcagagcaaa
      721 ctgagcaaag acccaaacga gaagagagat cacatggtcc tgctggagtt tgtgactgct
      781 gctggcatca ctctgggaat ggatgaactg tacaagggtg gaaccggagg tagcatggtt
      841 agcaagggtg aggaactctt tactggcgtg gtgcctatcc tcgtcgagct ggacggtgat
      901 gttaacggac acaagttctc cgtcagcgga gaaggtgagg gtgacgcaac ctacggcaaa
      961 ctcactctga agttcatttg taccactgga aagctgcctg tgccctggcc aactctggtg
     1021 actacactga cttacggagt gcagtgcttc tctcgctatc ctgatcacat gaagcagcat
     1081 gacttcttca agagcgctat gccagagggc tacatccaag agagaactat cttcttcaag
     1141 gatgacggaa actacaagac aagagcagag gttaagtttg agggagacac actggttaac
     1201 aggattgagc tgaaaggcat cgacttcaaa gaagatggaa acatcctggg acacaagctg
     1261 gagtacaatt ctatgcacaa gttctctcag gagcagatcg gagagaacat cgtgtgcaga
     1321 gtgatctgca caactggcca gatccctatt agggatctga gcgcagacat ttcccaagtc
     1381 ctgaaggaga agaggtccat caagaaagtt tggacatttg gtcgcaaccc tgcttgtgac
     1441 taccacctgg gaaacatttc caggctgtcc aacaagcact tccagattct cctcggagaa
     1501 gatggaaacc tgctcctcaa cgacatctcc acaaacggaa cttggctgaa cggacagaag
     1561 gtcgagaaga actccaacca gctgctgtct caaggcgacg agattactgt tggtgttggc
     1621 gtggagagcg acatcctgtc cctcgttatc ttcatcaacg ataagttcaa acagtgcctc
     1681 gaacagaaca aggtggatcg cggatccggt agcggtttcg caaacgagct gggaccaagg
     1741 ctgatgggta agggaggctc cggaggtaca ggagggtcag gagggactgg aggctctgga
     1801 ggtactggcg gttcaggagg aaccggtatg accgactaca ttagagatgg atctgctatc
     1861 aaagctctct cctttgccat catcctggct gaagctgacc tgagacacat cccacaagac
     1921 ctgcaaaggc tcgctgttcg cgtgattcac gcctgcggta tggtcgatgt cgccaacgat
     1981 ctggccttta gcgaaggtgc tggcaaggca ggtagaaacg ctctgctggc tggagcacct
     2041 atcctctgcg atgctaggat ggtggctgaa ggaatcacca ggtccaggct gcctgctgac
     2101 aatcgcgtca tctatacact gagcgatcct tccgttcctg agctggccaa gaagattgga
     2161 aacactcgct ctgcagcagc tctcgacctg tggctgccac acattgaagg aagcatcgtg
     2221 gcaattggca acgcaccaac tgccctgttt aggctcttcg aactcctgga cgcaggtgct
     2281 cctaagcctg ccctgatcat cggtatgcct gtcggatttg tcggtgcagc tgaatccaag
     2341 gacgagctgg cagccaacag cagaggcgtg ccctacgtga tcgtgagagg tagaagaggt
     2401 ggatctgcaa tgactgctgc agcagtgaat gctctggcct ccgagagaga gtaa
//
