LOCUS       sequence-311258-        3114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Signaling reporter island (SiRI) construct for spatially multiplexed
            imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA 
            indicator), V5 tag, and S3 protein scaffold..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Linghu C, Johnson SL, Valdes PA, Shemesh OA, Park WM, Park D, 
            Piatkevich KD, Wassie AT, Liu Y, An B, Barnes SA, Celiker OT, Yao 
            CC, Yu CJ, Wang R, Adamala KP, Bear MF, Keating AE, Boyden ES
  TITLE     Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling 
            Network Dynamics.
  JOURNAL   Cell. 2020 Nov 17. pii: S0092-8674(20)31399-4. doi: 
            10.1016/j.cell.2020.10.035.
  PUBMED    33232692
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Nov 17. pii: S0092-8674(20)31399-4. doi: 
            10.1016/j.cell.2020.10.035."
COMMENT     Sequence Label: pAAV-UBC-S3-ExRaiAKAR
FEATURES             Location/Qualifiers
     source          1..3114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2728..2769
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgccattca ttactgttgg acaggagaat agcacaagca ttgatctgta ctacgaagat
       61 catggcacag gcgtgcctgt tgtgctcatc cacggattcc cactcagcgg acactcctgg
      121 gaaaggcagt ccgctgcact cctggatgct ggatacaggg tgattaccta tgacaggaga
      181 ggattcggtc agagcagcca acccaccaca ggctacgact acgatacctt tgcagctgac
      241 ctgaacaccg tgctcgaaac cctcgatctg caggatgctg tcctggtggg attctctatg
      301 ggaactggtg aagttgctag gtacgtgagc agctacggaa cagctagaat cgctgcagtc
      361 gctttcctgg cttctctcga acctttcctg ctgaagacag acgacaatcc tgacggtgct
      421 gctcctcaag agttctttga tggtattgtt gctgccgtca aggctgacag gtatgccttc
      481 tacacaggat tcttcaacga cttctacaat ctggatgaga acctgggtac tcgcatttct
      541 gaggaggctg tcaggaactc ttggaacact gcagcctctg gtggtttctt tgcagcagca
      601 gctgcaccca ctacctggta cacagacttc agggcagata tcccaaggat tgacgtccca
      661 gctctgatcc tgcatggaac aggtgatcgc acactgccca tcgagaacac agctagagtc
      721 tttcacaaag cactgccttc cgcagaatac gttgaggtcg aaggtgcacc tcacggactg
      781 ctctggaccc acgctgagga ggttaacact gcactgctcg cattcctggc taaggctctg
      841 gaagcccaga aacagaagct gctgaccgag gtggagacat acgtcctctc tatcattccc
      901 tctggacctc tgaaagctga gattgctcag agactggaag acgtctttgc aggcaagaac
      961 accgacctgg aagttctgat ggaatggctg aagactaggc caattctgag ccctctcacc
     1021 aaaggaatcc tcggtttcgt ctttactctg acagtgccta gcgaaagagg tctgcaacgc
     1081 aggagattcg ttcagaatgc tctgaatggt aacggagatc caaacaacat ggacaaagct
     1141 gtgaaactgt accgcaaact caaacgcgag attaccttcc acggagctaa agaaatctct
     1201 ctcagctatt ctgcaggtgc actcgcttct tgcatgggac tgatctacaa tagaatgggt
     1261 gcagttacaa cagaagttgc attcggtctg gtgtgtgcaa cctgcgaaca gattgccgac
     1321 agccagcatc gctctcatag acaactcgag tctagaggag gttctggtgg aaccggtgga
     1381 agtggaggga caggaggttc aggtggtact ggtggctctg gaggaacagg aggctccgga
     1441 ggtacaggag ggtcaggagg gactggaggc tctggaggta ctggcggttc aggaggtacc
     1501 ggaggtctga gaagagctac actcgttgat ggaggtaccg gaggatctga gctgtctgca
     1561 atcaatgtgt acatcaaagc tgataagcag aagaacggca tcaaggctaa cttcaagatt
     1621 cgccacaaca ttgaggacgg aggcgtccag ctcgcttacc actaccagca gaacacacct
     1681 attggcgacg gtccagttct cctgcctgac aatcactacc tgtctgtgca gagcaaactg
     1741 agcaaagacc caaacgagaa gagagatcac atggtcctgc tggagtttgt gactgctgct
     1801 ggcatcactc tgggaatgga tgaactgtac aagggtggaa ccggaggtag catggttagc
     1861 aagggtgagg aactctttac tggcgtggtg cctatcctcg tcgagctgga cggtgatgtt
     1921 aacggacaca agttctccgt cagcggagaa ggtgagggtg acgcaaccta cggcaaactc
     1981 actctgaagt tcatttgtac cactggaaag ctgcctgtgc cctggccaac tctggtgact
     2041 acactgactt acggagtgca gtgcttctct cgctatcctg atcacatgaa gcagcatgac
     2101 ttcttcaaga gcgctatgcc agagggctac atccaagaga gaactatctt cttcaaggat
     2161 gacggaaact acaagacaag agcagaggtt aagtttgagg gagacacact ggttaacagg
     2221 attgagctga aaggcatcga cttcaaagaa gatggaaaca tcctgggaca caagctggag
     2281 tacaattcta tgcacaagtt ctctcaggag cagatcggag agaacatcgt gtgcagagtg
     2341 atctgcacaa ctggccagat ccctattagg gatctgagcg cagacatttc ccaagtcctg
     2401 aaggagaaga ggtccatcaa gaaagtttgg acatttggtc gcaaccctgc ttgtgactac
     2461 cacctgggaa acatttccag gctgtccaac aagcacttcc agattctcct cggagaagat
     2521 ggaaacctgc tcctcaacga catctccaca aacggaactt ggctgaacgg acagaaggtc
     2581 gagaagaact ccaaccagct gctgtctcaa ggcgacgaga ttactgttgg tgttggcgtg
     2641 gagagcgaca tcctgtccct cgttatcttc atcaacgata agttcaaaca gtgcctcgaa
     2701 cagaacaagg tggatcgcgg atccggtggt aagcctatcc ctaaccctct gctcggtctc
     2761 gattctaccg gaggttctgg aggttctggt ggtactggtg gttctggagg atctggtggt
     2821 acaggaggta gcggaggttc tggtggaacc tctgaagaac tgagagctgt cgcagacctg
     2881 caaagactca acattgaact ggccaggaag ctgctggaag ctgtggctag actgcaggaa
     2941 ctcaacattg atctcgtgag gaagacctcc gaactgaccg atgagaagac aatcagggag
     3001 gagatcagaa aggtgaagga agagtccaag aggattgtgg aggaagccga agaagaaatc
     3061 agaagagcca aggaagaatc caggtacatc gcagatgagt ccagaggaag ctaa
//
