LOCUS       sequence-311259-        3558 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Signaling reporter island (SiRI) construct for spatially multiplexed
            imaging. Contains GFP-based fluorescent reporter cAMPr (cAMP 
            indicator), HA tag, and S2 protein scaffold..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Linghu C, Johnson SL, Valdes PA, Shemesh OA, Park WM, Park D, 
            Piatkevich KD, Wassie AT, Liu Y, An B, Barnes SA, Celiker OT, Yao 
            CC, Yu CJ, Wang R, Adamala KP, Bear MF, Keating AE, Boyden ES
  TITLE     Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling 
            Network Dynamics.
  JOURNAL   Cell. 2020 Nov 17. pii: S0092-8674(20)31399-4. doi: 
            10.1016/j.cell.2020.10.035.
  PUBMED    33232692
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Nov 17. pii: S0092-8674(20)31399-4. doi: 
            10.1016/j.cell.2020.10.035."
COMMENT     Sequence Label: pAAV-UBC-S2-cAMPr
FEATURES             Location/Qualifiers
     source          1..3558
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2208..2229
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3220..3246
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atgaagccca ccacaatcag cctgctgcag aagtacaagc aggagaagaa gcggttcgcc
       61 accatcacag cttacgacta ctccttcgcc aagctgtttg ctgatgaggg actgaacgtg
      121 atgctggtgg gagactctct gggcatgacc gtgcagggac acgacagcac cctgccagtg
      181 acagtggccg atatcgctta ccacacagcc gctgtgagga gaggagcccc taactgcctg
      241 ctgctggctg atctgccctt catggcctac gctacccctg agcaggcctt tgagaacgcc
      301 gctacagtga tgagggccgg cgctaacatg gtgaagatcg agggcggaga gtggctggtg
      361 gagaccgtgc agatgctgac agagagagcc gtgccagtgt gcggacacct gggactgacc
      421 ccacagtctg tgaacatctt tggcggatac aaggtgcagg gaaggggact gctggctgga
      481 ctgcagctgc tgtccgacgc tctggctctg gaggctgctg gagctcagct gctggtgctg
      541 gagtgcgtgc ctgtggagct ggctaagcgc atcacagagg ccctggctat cccagtgatc
      601 ggaatcggag ctggaaacgt gaccgacgga cagatcctgg tcatgcacga tgctttcggc
      661 atcacaggcg gacacatccc aaagttcgcc aagaactttc tggctgagac cggcgatatc
      721 agggctgctg tgcgccagta catggctgag gtggagtccg gagtgtaccc aggagaggag
      781 cacagctttc actctagagg aggttctggt ggaaccggtg gaagtggagg gacaggaggt
      841 tcaggtggta ctggtggctc tggaggaaca ggaggctccg gaggtacagg agggtcagga
      901 gggactggag gctctggagg tactggcggt tcaggaggta ccatgggcaa cgccgccgcc
      961 gccaagaagg gcagcgagca ggagagcgtg aaagagttcc tagccaaagc caaggaagat
     1021 ttcctgaaaa aatgggagac cccttctcag aatacagccc agttggatca gtttgataga
     1081 atcaagaccc ttggcaccgg ctcctttggg cgagtgatgc tggtgaagca caaggagagt
     1141 gggaaccact acgccatgaa gatcttagac aagcagaagg tggtgaagct aaagcagatc
     1201 gagcacactc tgaatgagaa gcgcatcctg caggccgtca acttcccgtt cctggtcaaa
     1261 cttgaattct ccttcaagga caactcaaac ctgtacatgg tcatggagta tgtagctggt
     1321 ggcgagatgt tctcccacct acggcggatt ggaaggttca gcgagcccca tgcccgtttc
     1381 tacgcggcgc agatcgtcct gacctttgag tatctgcact ccctggacct catctaccgg
     1441 gacctgaagc ccgagaatct tctcatcgac cagcagggct atattcaggt gacagacttc
     1501 ggttttgcca agcgtgtgaa aggccgtact tggaccttgt gtgggacccc tgagtacttg
     1561 gcccccgaga ttatcctgag caaaggctac aacaaggctg tggactggtg ggctctcgga
     1621 gtcctcatct acgagatggc tgctggttac ccacccttct tcgctgacca gcctatccag
     1681 atctatgaga aaatcgtctc tgggaaggtg cggttcccat cccacttcag ctctgacttg
     1741 aaggacctgc tgcggaacct tctgcaagtg gatctcacca agcgctttgg gaacctcaag
     1801 aacggggtca atgacatcaa gaaccacaag tggtttgcca cgactgactg gattgccatc
     1861 tatcagagaa aggtggaagc tcccttcata ccaaagttta aaggccctgg ggacacgagt
     1921 aactttgacg actatgagga ggaagagatc cgggtctcca tcaatgagaa gtgtggcaag
     1981 gagtttactg agtttcccgg gaacgtctat atcaaggccg acgagcagaa gaacggcatc
     2041 aaggcgtact tcaagatccg ccacaacatc gagggcggcg gcgtgcagct cgcctaccac
     2101 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     2161 agcgtgcagt ccatgctttc gaaagacccc aacgagaagc gcgatcacat ggtcctgctg
     2221 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggtacc
     2281 ggagggagcg aatccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     2341 caggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggtgag
     2401 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     2461 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     2521 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacatccag
     2581 gagcgcacca tcttcttcaa gggcgacggc aactacaaga cccgcgccga ggtgaagttc
     2641 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     2701 aacatcctgg ggcacaagct ggagtacaac accgcccccc ccgcatgcaa gggccggcgg
     2761 cgacgcgggg ccatcagcgc tgaggtctac accgaggagg atgccgcgtc ctatgttcgg
     2821 aaggttatac cgaaagatta taagacaatg gctgctttag ctaaagccat tgaaaagaac
     2881 gtactgtttt cacatcttga tgataacgag agaagtgaca tttttgacgc catgttcccg
     2941 gtttccttta ttgctggaga gactgttatt cagcagggtg acgaagggga taacttctac
     3001 gtgattgacc aaggagagat ggacgtctat gtcaacaatg aatgggcaac cagtgttggg
     3061 gaaggaggga gcttcgggga gcttgctctg atttacggga ctcctcgagc ggccactgtc
     3121 aaggccaaga cgaacgtgaa actgtggggc attgaccggg acagctacag aaggatcctc
     3181 atgggaagca cgctgagaaa gcggaagatg ggatccggtt acccatacga cgttccagac
     3241 tacgctggag gttctggagg ttctggtggt actggtggtt ctggaggatc tggtggtaca
     3301 ggaggtagcg gaggttctgg tggaaccgga agcagagtgt acgagtctga gaaactggct
     3361 agagaggcag acaagctggc tcagaagagc gaggatatgg ctagagaggc agataagcag
     3421 gccaggaggg ctgaggaaag acctgacaga gaagagattg ctaggctcgc tgccatcatt
     3481 gctaggatgg tggctctcaa ctccagaatc gcaatgctca tggcacgcat gatcatgctg
     3541 aactctcaag agagctaa
//
