LOCUS       sequence-311260-       16986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant mitoTALEN vector to cut Brassica CMS gene orf125 (orf138).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16986)
  AUTHORS   Kazama T, Okuno M, Watari Y, Yanase S, Koizuka C, Tsuruta Y, Sugaya 
            H, Toyoda A, Itoh T, Tsutsumi N, Toriyama K, Koizuka N, Arimura SI
  TITLE     Curing cytoplasmic male sterility via TALEN-mediated mitochondrial 
            genome editing.
  JOURNAL   Nat Plants. 2019 Jul;5(7):722-730. doi: 10.1038/s41477-019-0459-z. 
            Epub 2019 Jul 8.
  PUBMED    31285556
REFERENCE   2  (bases 1 to 16986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-019-0459-z"; journalName: "Nat Plants"; date: 
            "2019-07"; volume: "5"; issue: "7"; pages: "722-730"
COMMENT     Sequence Label: pK7WG2-mitoTAL1-orf125
FEATURES             Location/Qualifiers
     source          1..16986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        127..306
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             340..1134
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(394..413)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1004..1023
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1174..1426
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1519..1543
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2064..2855
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3101..3689
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3590..3609
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3843..3860
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3875..4015
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3941..3960)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4101..4123
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4359..4553
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4619..5692)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6121..6750)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8051..8075
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        8159..8504
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    8524..8579
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     protein_bind    8776..8800
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8803..8829
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             11383..11970
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     protein_bind    complement(12008..12028)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      12033..12281
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        12866..13211
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    13231..13286
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     protein_bind    13472..13492
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             13495..13539
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    complement(16717..16741)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gacgtcgggc cctctagagg atccccgggt accgcgaatt atcgatcatg agcggagaat
       61 taagggagtc acgttatgac ccccgccgat gacgcgggac aagccgtttt acgtttggaa
      121 ctgacagaac cgcaacgttg aaggagccac tgagccgcgg gtttctggag tttaatgagc
      181 taagcacata cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg tcactatcag
      241 ctagcaaata tttcttgtca aaaatgctcc actgacgttc cataaattcc cctcggtatc
      301 caattagagt ctcatattca ctctcaactc gatcgaggca tgattgaaca agatggattg
      361 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
      421 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
      481 tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc aagacgaggc agcgcggcta
      541 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
      601 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
      661 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
      721 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
      781 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
      841 gccgaactgt tcgccaggct caaggcgcgg atgcccgacg gcgaggatct cgtcgtgacc
      901 cacggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
      961 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     1021 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     1081 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     1141 ctctggggtt cggactctag ctagagtcaa gcagatcgtt caaacatttg gcaataaagt
     1201 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     1261 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     1321 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     1381 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgacc ggcatgcaag
     1441 ctgataattc aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag
     1501 ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct
     1561 ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga
     1621 cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt
     1681 cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca
     1741 tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt
     1801 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     1861 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     1921 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     1981 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     2041 gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta tcgactcaac
     2101 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     2161 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     2221 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     2281 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     2341 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     2401 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     2461 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     2521 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     2581 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     2641 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     2701 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     2761 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     2821 tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc
     2881 cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct
     2941 cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc
     3001 tacacaaatt gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3061 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3121 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3181 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3241 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3301 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3361 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3421 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3481 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3541 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3601 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3661 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3721 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3781 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3841 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     3901 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     3961 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
     4021 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     4081 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     4141 gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg
     4201 cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg
     4261 ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg
     4321 ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt
     4381 taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta
     4441 tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg
     4501 ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga
     4561 gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt
     4621 aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc
     4681 cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct
     4741 tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg
     4801 tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa
     4861 aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt
     4921 tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg
     4981 gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata
     5041 ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt
     5101 ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc
     5161 agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc
     5221 cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt
     5281 aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa
     5341 gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca
     5401 cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat
     5461 ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt
     5521 gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt
     5581 ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat
     5641 cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc
     5701 ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc
     5761 tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc
     5821 ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc
     5881 tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt
     5941 ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc
     6001 gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc
     6061 tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac
     6121 tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc
     6181 ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg
     6241 ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc
     6301 accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc
     6361 atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc
     6421 gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc
     6481 caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt
     6541 cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg
     6601 cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc
     6661 cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc
     6721 tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact
     6781 catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac
     6841 ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc
     6901 ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc
     6961 ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac
     7021 ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg
     7081 cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg
     7141 cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca
     7201 cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg
     7261 cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct
     7321 tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa
     7381 tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct
     7441 ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt
     7501 cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta
     7561 ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg
     7621 aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt
     7681 cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt
     7741 aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa
     7801 aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt
     7861 cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg
     7921 gttggatcaa agtactttra wsymswkkrr arymctktrr tkggcatgca catacaaatg
     7981 gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct
     8041 tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc
     8101 gggaaacgac aatctgatcc aagctcaagc taagctcctc agaagaccag agggctattg
     8161 agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct
     8221 gtcacttcat cgaaaggaca gtagaaaagg aaggtggctc ctacaaatgc catcattgcg
     8281 ataaaggaaa ggctatcgtt caagatgcct ctaccgacag tggtcccaaa gatggacccc
     8341 cacccacgag gaacatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     8401 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
     8461 acccttcctc tatataagga agttcatttc atttggagag gacaggcttc ttgagatcct
     8521 tcaacaatta ccaacaacaa caaacaacaa acaacattac aattactatt tacaattaca
     8581 gtcgacccac gcgtccgata atttccccaa tttctcagag agactcaaaa tcaccaccac
     8641 caccaaatcg ccgccatgtt taaacaagct tctcgtctcc tctcccgatc tgtcgccgcc
     8701 gcatcttcca aatcggtgac gactcgtgcc ttttcaacgg aacttccatc gacgctcgat
     8761 tccgctagtg atatcacaag tttgtacaaa aaagcaggct tttacccata cgatgttcca
     8821 gattacgctg atatcgccga tctacgcacg ctcggctaca gccagcagca acaggagaag
     8881 atcaaaccga aggttcgttc gacagtggcg cagcaccacg aggcactggt cggccacggg
     8941 tttacacacg cgcacatcgt tgcgttaagc caacacccgg cagcgttagg gaccgtcgct
     9001 gtcaagtatc aggacatgat cgcagcgttg ccagaggcga cacacgaagc gatcgttggc
     9061 gtcggcaaac agtggtccgg cgcacgcgct ctggaggcct tgctcacggt ggcgggagag
     9121 ttgagaggtc caccgttaca gttggacaca ggccaacttc tcaagattgc aaaacgtggc
     9181 ggcgtgaccg cagtggaggc agtgcatgca tggcgcaatg cactgacggg tgccccgctc
     9241 aacctgaccc cagaccaggt tgtggccatc gccagcaaca taggtggcaa gcaggccctc
     9301 gaaaccgtcc agagactgtt accggttctc tgccaggacc acggcctgac cccagaacag
     9361 gttgtggcca tcgccagcaa cataggtggc aagcaggccc tcgaaaccgt ccagagactg
     9421 ttaccggttc tctgccaggc ccacggcctg acccccgacc aggttgtcgc tattgctagt
     9481 aacggcggag gcaaacaggc gctggaaaca gttcagcgcc tcttgccggt cttgtgtcag
     9541 gcccacggcc tgaccccagc ccaagttgtc gcgattgcaa gcaacaacgg aggcaaacaa
     9601 gccttagaaa cagtccagag attgttgccg gtgctgtgcc aagaccacgg cctgacccca
     9661 gaccaagttg tcgcgattgc aagcaacaac ggaggcaaac aagccttaga aacagtccag
     9721 agattgttgc cggtgctgtg ccaagaccac ggcctgaccc cggaacaggt ggttgcaatc
     9781 gcgtcacacg atgggggaaa gcaggcccta gaaaccgttc agcgactcct gcccgtcctg
     9841 tgccaggccc acggcctgac cccggaccag gtggttgcaa tcgcgtcaca cgatggggga
     9901 aagcaggccc tagaaaccgt tcagcgactc ctgcccgtcc tgtgccaggc ccacggcctg
     9961 accccggccc aggtggttgc aatcgcgtca cacgatgggg gaaagcaggc cctagaaacc
    10021 gttcagcgac tcctgcccgt cctgtgccag gaccacggcc tgaccccaga ccaggttgtg
    10081 gccatcgcca gcaacatagg tggcaagcag gccctcgaaa ccgtccagag actgttaccg
    10141 gttctctgcc aggaccacgg cctgaccccc gaacaggttg tcgctattgc tagtaacggc
    10201 ggaggcaaac aggcgctgga aacagttcag cgcctcttgc cggtcttgtg tcaggcccac
    10261 ggcctgaccc cagaccaggt tgtggccatc gccagcaaca taggtggcaa gcaggccctc
    10321 gaaaccgtcc agagactgtt accggttctc tgccaggccc acggcctgac ccccgcccag
    10381 gttgtcgcta ttgctagtaa cggcggaggc aaacaggcgc tggaaacagt tcagcgcctc
    10441 ttgccggtct tgtgtcagga ccacggcctg acccccgacc aggttgtcgc tattgctagt
    10501 aacggcggag gcaaacaggc gctggaaaca gttcagcgcc tcttgccggt cttgtgtcag
    10561 gaccacggcc tgacccccga acaggttgtc gctattgcta gtaacggcgg aggcaaacag
    10621 gcgctggaaa cagttcagcg cctcttgccg gtcttgtgtc aggcccacgg cctgacccca
    10681 gaccaagttg tcgcgattgc aagcaacaac ggaggcaaac aagccttaga aacagtccag
    10741 agattgttgc ctgtgctgtg ccaagcccac ggcctgaccc cagcccaagt tgtcgcgatt
    10801 gcaagcaaca acggaggcaa acaagcctta gaaacagtcc agagattgtt gccggtgctg
    10861 tgccaagacc acggcctgac cccggaccag gtggttgcaa tcgcgtcaca cgatggggga
    10921 aagcaggccc tagaaaccgt tcagcgactc ctgcccgtcc tgtgccagga ccacggcctg
    10981 acccccgaac aggttgtcgc tattgctagt aacggcggag gcaaacaggc gctggaaaca
    11041 gttcagcgcc tcttgccggt cttgtgtcag gcccacggcc tgaccccaga ccaggttgtg
    11101 gccatcgcca gcaacatagg tggcaagcag gccctcgaaa ccgtccagag actgttaccg
    11161 gttctctgcc aggcccacgg cctgacccct gagcaggtag tggctattgc atccaacata
    11221 gggggcagac ccgcactgga gtcaattgtt gcccagttat ctcgccctga tccggcgttg
    11281 gccgcgttga ccaacgacca cctcgtcgcc ttggcctgcc tcggcgggcg tcctgcgctg
    11341 gatgcagtga aaaagggatt gggggatcct atcagccgtt cccagctggt gaagtccgag
    11401 ctggaggaga agaaatccga gttgaggcac aagctgaagt acgtgcccca cgagtacatc
    11461 gagctgatcg agatcgcccg gaacagcacc caggaccgta tcctggagat gaaggtgatg
    11521 gagttcttca tgaaggtgta cggctacagg ggcaagcacc tgggcggctc caggaagccc
    11581 gacggcgcca tctacaccgt gggctccccc atcgactacg gcgtgatcgt ggacaccaag
    11641 gcctactccg gcggctacaa cctgcccatc ggccaggccg acgaaatgca gaggtacgtg
    11701 gaggagaacc agaccaggaa caagcacatc aaccccaacg agtggtggaa ggtgtacccc
    11761 tccagcgtga ccgagttcaa gttcctgttc gtgtccggcc acttcaaggg caactacaag
    11821 gcccagctga ccaggctgaa ccacatcacc aactgcaacg gcgccgtgct gtccgtggag
    11881 gagctcctga tcggcggcga gatgatcaag gccggcaccc tgaccctgga ggaggtgagg
    11941 aggaagttca acaacggcga gatcaacttc gcggccgact gataactcga gcgatcctct
    12001 agacacccaa cttttctata caaagttgtg aatatgaaga tgaagatgaa atatttggtg
    12061 tgtcaaataa aaagcttgtg tgcttaagtt tgtgtttttt tcttggcttg ttgtgttatg
    12121 aatttgtggc tttttctaat attaaatgaa tgtaagatct cattataatg aataaacaaa
    12181 tgtttctata atccattgtg aatgttttgt tggatctctt ctgcagcata taactactgt
    12241 atgtgctatg gtatggacta tggaatatga ttaaagataa gatgggctca tagagtaaaa
    12301 cgaggcgagg gacctataaa cctcccttca tcatgctatt tcatgatcta ttttataaaa
    12361 taaagatgta gaaaaaagta agcgtaataa ccgcaaaaca aatgatttaa aacatggcac
    12421 ataatgagga gattaagttc ggtttacgtt tattttagta ctaattgtaa cgtgagacta
    12481 cgtatcggga atcgcctaat taaagcatta atgcgaacct gattagattc accgaccctc
    12541 ctatcgtgtc gacctttctg tttcttagaa ttttttggta gtctatgtac taataatgtc
    12601 agcttcgtat ttatttcata agcaatttgc atttgcaatt tgttttttac ttttattttt
    12661 attgtattgt ggaatgtgga ctcgtaccaa catgaagtta tataccacca aaaaaattac
    12721 agttagtcaa aagattcacg agtgagagct acttatgatt gtcttttacg tatatgtcta
    12781 attgtctatt tgctcaataa tctttgtact ttcttttgtc gttgataaat ctagaggatc
    12841 ccccctcaga agaccagagg gctattgaga cttttcaaca aagggtaata tcgggaaacc
    12901 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
    12961 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctcta
    13021 ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa aaagaagacg
    13081 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
    13141 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
    13201 tggagaggac aggcttcttg agatccttca acaattacca acaacaacaa acaacaaaca
    13261 acattacaat tactatttac aattacagtc gacccacgcg tccgataatt tccccaattt
    13321 ctcagagaga ctcaaaatca ccaccaccac caaatcgccg ccatgtttaa acaagcttct
    13381 cgtctcctct cccgatctgt cgccgccgca tcttccaaat cggtgacgac tcgtgccttt
    13441 tcaacggaac ttccatcgac gctcgattcc acaactttgt ataataaagt tgataaggag
    13501 accgccgctg ccaagttcga gagacagcac atggacagca tcgatatcgc cgatctacgc
    13561 acgctcggct acagccagca gcaacaggag aagatcaaac cgaaggttcg ttcgacagtg
    13621 gcgcagcacc acgaggcact ggtcggccac gggtttacac acgcgcacat cgttgcgtta
    13681 agccaacacc cggcagcgtt agggaccgtc gctgtcaagt atcaggacat gatcgcagcg
    13741 ttgccagagg cgacacacga agcgatcgtt ggcgtcggca aacagtggtc cggcgcacgc
    13801 gctctggagg ccttgctcac ggtggcggga gagttgagag gtccaccgtt acagttggac
    13861 acaggccaac ttctcaagat tgcaaaacgt ggcggcgtga ccgcagtgga ggcagtgcat
    13921 gcatggcgca atgcactgac gggtgccccg ctcaacctga ccccagacca agttgtcgcg
    13981 attgcaagca acaacggagg caaacaagcc ttagaaacag tccagagatt gttgccggtg
    14041 ctgtgccaag accacggcct gaccccagaa caagttgtcg cgattgcaag caacaacgga
    14101 ggcaaacaag ccttagaaac agtccagaga ttgttgccgg tgctgtgcca agcccacggc
    14161 ctgacccccg accaggttgt cgctattgct agtaacggcg gaggcaaaca ggcgctggaa
    14221 acagttcagc gcctcttgcc ggtcttgtgt caggcccacg gcctgacccc cgcccaggtt
    14281 gtcgctattg ctagtaacgg cggaggcaaa caggcgctgg aaacagttca gcgcctcttg
    14341 ccggtcttgt gtcaggacca cggcctgacc ccggaccagg tggttgcaat cgcgtcacac
    14401 gatgggggaa agcaggccct agaaaccgtt cagcgactcc tgcccgtcct gtgccaggac
    14461 cacggcctga ccccagaaca agttgtcgcg attgcaagca acaacggagg caaacaagcc
    14521 ttagaaacag tccagagatt gttgccggtg ctgtgccaag cccacggcct gacccccgac
    14581 caggttgtcg ctattgctag taacggcgga ggcaaacagg cgctggaaac agttcagcgc
    14641 ctcttgccgg tcttgtgtca ggcccacggc ctgaccccag cccaggttgt ggccatcgcc
    14701 agcaacatag gtggcaagca ggccctcgaa accgtccaga gactgttacc ggttctctgc
    14761 caggaccacg gcctgacccc agaccaggtt gtggccatcg ccagcaacat aggtggcaag
    14821 caggccctcg aaaccgtcca gagactgtta ccggttctct gccaggacca cggcctgacc
    14881 ccagaacagg ttgtggccat cgccagcaac ataggtggca agcaggccct cgaaaccgtc
    14941 cagagactgt taccggttct ctgccaggcc cacggcctga ccccggacca ggtggttgca
    15001 atcgcgtcac acgatggggg aaagcaggcc ctagaaaccg ttcagcgact cctgcccgtc
    15061 ctgtgccagg cccacggcct gaccccagcc caggttgtgg ccatcgccag caacataggt
    15121 ggcaagcagg ccctcgaaac cgtccagaga ctgttaccgg ttctctgcca ggaccacggc
    15181 ctgaccccag accaggttgt ggccatcgcc agcaacatag gtggcaagca ggccctcgaa
    15241 accgtccaga gactgttacc ggttctctgc caggaccacg gcctgacccc cgaacaggtt
    15301 gtcgctattg ctagtaacgg cggaggcaaa caggcgctgg aaacagttca gcgcctcttg
    15361 ccggtcttgt gtcaggccca cggcctgacc ccagaccaag ttgtcgcgat tgcaagcaac
    15421 aacggaggca aacaagcctt agaaacagtc cagagattgt tgcctgtgct gtgccaagcc
    15481 cacggcctga cccccgccca ggttgtcgct attgctagta acggcggagg caaacaggcg
    15541 ctggaaacag ttcagcgcct cttgccggtc ttgtgtcagg accacggcct gacccccgac
    15601 caggttgtcg ctattgctag taacggcgga ggcaaacagg cgctggaaac agttcagcgc
    15661 ctcttgccgg tcttgtgtca ggaccacggc ctgaccccag aacaagttgt cgcgattgca
    15721 agcaacaacg gaggcaaaca agccttagaa acagtccaga gattgttgcc ggtgctgtgc
    15781 caagcccacg gcctgacccc agaccaagtt gtcgcgattg caagcaacaa cggaggcaaa
    15841 caagccttag aaacagtcca gagattgttg cctgtgctgt gccaagccca cggcctgacc
    15901 cctgagcagg tagtggctat tgcatccaac ggagggggca gacccgcact ggagtcaatt
    15961 gttgcccagt tatctcgccc tgatccggcg ttggccgcgt tgaccaacga ccacctcgtc
    16021 gccttggcct gcctcggcgg gcgtcctgcg ctggatgcag tgaaaaaggg attgggggat
    16081 cctatcagcc gttcccagct ggtgaagtcc gagctggagg agaagaaatc cgagttgagg
    16141 cacaagctga agtacgtgcc ccacgagtac atcgagctga tcgagatcgc ccggaacagc
    16201 acccaggacc gtatcctgga gatgaaggtg atggagttct tcatgaaggt gtacggctac
    16261 aggggcaagc acctgggcgg ctccaggaag cccgacggcg ccatctacac cgtgggcccc
    16321 cccatcgact acggcgtgat cgtggacacc aaggcctact ccggcggcta caacctgccc
    16381 atcggccagg ccgacgaaat gcagaggtac gtggaggaga accagaccag gaacaagcac
    16441 atcaacccca acgagtggtg gaaggtgtac ccctccagcg tgaccgagtt caagttcctg
    16501 ttcgtgtccg gccacttcaa gggcaactac aaggcccagc tgaccaggct gaaccacatc
    16561 accaactgca acggcgccgt gctgtccgtg gaggagctcc tgatcggcgg cgagatgatc
    16621 aaggccggca ccctgaccct ggaggaggtg aggaggaagt tcaacaacgg cgagatcaac
    16681 ttcgcggccg actgataact cgagcgatcc tctagaaccc agctttcttg tacaaagtgg
    16741 tgatatcccg cggccatgct agagtccgca aaaatcacca gtctctctct acaaatctat
    16801 ctctctctat ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt
    16861 cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt
    16921 gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacctgcag
    16981 gcatgc
//
