LOCUS       sequence-311263-        3273 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Signaling reporter island (SiRI) construct for spatially multiplexed
            imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK 
            indicator), VSV-G tag, and S2 protein scaffold..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Linghu C, Johnson SL, Valdes PA, Shemesh OA, Park WM, Park D, 
            Piatkevich KD, Wassie AT, Liu Y, An B, Barnes SA, Celiker OT, Yao 
            CC, Yu CJ, Wang R, Adamala KP, Bear MF, Keating AE, Boyden ES
  TITLE     Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling 
            Network Dynamics.
  JOURNAL   Cell. 2020 Nov 17. pii: S0092-8674(20)31399-4. doi: 
            10.1016/j.cell.2020.10.035.
  PUBMED    33232692
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Nov 17. pii: S0092-8674(20)31399-4. doi: 
            10.1016/j.cell.2020.10.035."
COMMENT     Sequence Label: pAAV-UBC-S2-RAB_EKARev
FEATURES             Location/Qualifiers
     source          1..3273
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1807..2154
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             2929..2961
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
ORIGIN
        1 atgaagccca ccacaatcag cctgctgcag aagtacaagc aggagaagaa gcggttcgcc
       61 accatcacag cttacgacta ctccttcgcc aagctgtttg ctgatgaggg actgaacgtg
      121 atgctggtgg gagactctct gggcatgacc gtgcagggac acgacagcac cctgccagtg
      181 acagtggccg atatcgctta ccacacagcc gctgtgagga gaggagcccc taactgcctg
      241 ctgctggctg atctgccctt catggcctac gctacccctg agcaggcctt tgagaacgcc
      301 gctacagtga tgagggccgg cgctaacatg gtgaagatcg agggcggaga gtggctggtg
      361 gagaccgtgc agatgctgac agagagagcc gtgccagtgt gcggacacct gggactgacc
      421 ccacagtctg tgaacatctt tggcggatac aaggtgcagg gaaggggact gctggctgga
      481 ctgcagctgc tgtccgacgc tctggctctg gaggctgctg gagctcagct gctggtgctg
      541 gagtgcgtgc ctgtggagct ggctaagcgc atcacagagg ccctggctat cccagtgatc
      601 ggaatcggag ctggaaacgt gaccgacgga cagatcctgg tcatgcacga tgctttcggc
      661 atcacaggcg gacacatccc aaagttcgcc aagaactttc tggctgagac cggcgatatc
      721 agggctgctg tgcgccagta catggctgag gtggagtccg gagtgtaccc aggagaggag
      781 cacagctttc actctagagg aggttctggt ggaaccggtg gaagtggagg gacaggaggt
      841 tcaggtggta ctggtggctc tggaggaaca ggaggctccg gaggtacagg agggtcagga
      901 gggactggag gctctggagg tactggcggt tcaggaggta ccatggtgtc caagagcgaa
      961 gaagtgatca aggagttcat gaggttcaag gtcaggctgg aaggaagcat gaatggccac
     1021 gagttcgaga ttgagggaga gggtgaaggc agaccttatg aaggaacaca gactgctaaa
     1081 ctcaaagtga ctaaaggtgg tcctctccca ttcgcttggg acatcctgtc tccacagatc
     1141 atgtatggca gcaaagcata cgtcaagcac ccagccgacg tgccagatta catgaaactg
     1201 agcttcccag agggattcaa atgggaaagg gtgatgcact tcgaggatgg aggactcgtc
     1261 acagttaccc aagacacttc tctccaagac ggaaccctga tctacaaggt caagatgaga
     1321 ggcactaact ttccacccga tggacccgtt atgcagaaga agactctcgg ttgggactat
     1381 gcaactgaga gactgtaccc agaagacgga gtgctcaagg gtgaactgct cggtagactc
     1441 aagctcaagg acggtggcct gaacctggtg gagttcaaga caatctacat ggctaagaag
     1501 ccagtccagc tccctggcta ctacttcgtg gacaccaaac tgggcattac atctcacaat
     1561 gaagactaca ccatcgtgga gcagtacgaa cgctccgaag gtaggcatca cctgggacat
     1621 ggaactggtt ccaggtccat ggctgatgaa gagaagctgc cacctggatg ggagaagaga
     1681 atgtctcgca gctctggcag agtttactac ttcaaccaca tcacaaacgc ctcccagtgg
     1741 gaaaggccat ccggtaactc ttcctccggt ggcaagaacg gacaaggcga acctgctaga
     1801 ggaaccagtg ctggtggtag tgctggtggt agtgctggtg gtagtgctgg tggtagtgct
     1861 ggtggttccg gcagtgctgg tggtagtgct ggtggtagta ccagtgctgg tggtagtgct
     1921 ggtggtagtg ctggtggtag tgctggtggt agtgctggtg gttccggcag tgctggtggt
     1981 agtgctggtg gtagtaccag tgctggtggt agtgctggtg gtagtgctgg tggtagtgct
     2041 ggtggtagtg ctggtggttc cggcagtgct ggtggtagtg ctggtggtag taccagtgct
     2101 ggtggtagtg ctggtggtag tgctggtggt agtgctggtg gtagtgctgg tggttctggt
     2161 ccagatgtgc ctcgcactcc tgtggacaag gctaaactga gcttccagtt tccaggtact
     2221 gctagcagcg aagataacaa catggctacc atcaaggagt tcatgagatt caaagttcgc
     2281 atggagggca gcatgaacgg acacgagttc gaaatcgagg gtgaaggtga gggcagacct
     2341 tacgaaggta ctcagactgc aaagctgaag gtgaccaagg gtggacctct gccatttgcc
     2401 tgggacatcc tctctccaca gttcatgtac ggcagcgagg catacgtcag gcatcctgct
     2461 gatatccctg attacaagaa actcccattt cctgagggat tcaaatggga gagagtcatg
     2521 aactttgaag acggtggtct ggtcactgtg acacaggact cttctctgca ggacggaacc
     2581 ctcatctgca aagttaagat gagaggaaca aactttcctc cagacggacc cgtgatgcag
     2641 aagaagacta tgggctggga ggcttctaca gagatgctgt accctgagga cggagtgctg
     2701 aagggtcatt cctaccaagc tctcaagctg aaggacggtg gccactatct cgtggagttc
     2761 gagactatct acatggcaaa gaaacccgtg caactgcctg gtgactactg tgttgacaca
     2821 aagctggaca ttacaagcca caacgaggat tacaccatcg ttgagcaata cggaaggtct
     2881 gagggcaggc ataggctcgg catggatgag aggtacaagg gatccggtta cactgacatt
     2941 gagatgaaca gactcggtaa gggaggttct ggaggttctg gtggtactgg tggttctgga
     3001 ggatctggtg gtacaggagg tagcggaggt tctggtggaa ccggaagcag agtgtacgag
     3061 tctgagaaac tggctagaga ggcagacaag ctggctcaga agagcgagga tatggctaga
     3121 gaggcagata agcaggccag gagggctgag gaaagacctg acagagaaga gattgctagg
     3181 ctcgctgcca tcattgctag gatggtggct ctcaactcca gaatcgcaat gctcatggca
     3241 cgcatgatca tgctgaactc tcaagagagc taa
//
