LOCUS       sequence-311265-        3864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Signaling reporter island (SiRI) construct for spatially multiplexed
            imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK 
            indicator), VSV-G tag, and S3 protein scaffold..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Linghu C, Johnson SL, Valdes PA, Shemesh OA, Park WM, Park D, 
            Piatkevich KD, Wassie AT, Liu Y, An B, Barnes SA, Celiker OT, Yao 
            CC, Yu CJ, Wang R, Adamala KP, Bear MF, Keating AE, Boyden ES
  TITLE     Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling 
            Network Dynamics.
  JOURNAL   Cell. 2020 Nov 17. pii: S0092-8674(20)31399-4. doi: 
            10.1016/j.cell.2020.10.035.
  PUBMED    33232692
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Nov 17. pii: S0092-8674(20)31399-4. doi: 
            10.1016/j.cell.2020.10.035."
COMMENT     Sequence Label: pAAV-UBC-S3-RAB_EKARev
FEATURES             Location/Qualifiers
     source          1..3864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2365..2712
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             3487..3519
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
ORIGIN
        1 atgccattca ttactgttgg acaggagaat agcacaagca ttgatctgta ctacgaagat
       61 catggcacag gcgtgcctgt tgtgctcatc cacggattcc cactcagcgg acactcctgg
      121 gaaaggcagt ccgctgcact cctggatgct ggatacaggg tgattaccta tgacaggaga
      181 ggattcggtc agagcagcca acccaccaca ggctacgact acgatacctt tgcagctgac
      241 ctgaacaccg tgctcgaaac cctcgatctg caggatgctg tcctggtggg attctctatg
      301 ggaactggtg aagttgctag gtacgtgagc agctacggaa cagctagaat cgctgcagtc
      361 gctttcctgg cttctctcga acctttcctg ctgaagacag acgacaatcc tgacggtgct
      421 gctcctcaag agttctttga tggtattgtt gctgccgtca aggctgacag gtatgccttc
      481 tacacaggat tcttcaacga cttctacaat ctggatgaga acctgggtac tcgcatttct
      541 gaggaggctg tcaggaactc ttggaacact gcagcctctg gtggtttctt tgcagcagca
      601 gctgcaccca ctacctggta cacagacttc agggcagata tcccaaggat tgacgtccca
      661 gctctgatcc tgcatggaac aggtgatcgc acactgccca tcgagaacac agctagagtc
      721 tttcacaaag cactgccttc cgcagaatac gttgaggtcg aaggtgcacc tcacggactg
      781 ctctggaccc acgctgagga ggttaacact gcactgctcg cattcctggc taaggctctg
      841 gaagcccaga aacagaagct gctgaccgag gtggagacat acgtcctctc tatcattccc
      901 tctggacctc tgaaagctga gattgctcag agactggaag acgtctttgc aggcaagaac
      961 accgacctgg aagttctgat ggaatggctg aagactaggc caattctgag ccctctcacc
     1021 aaaggaatcc tcggtttcgt ctttactctg acagtgccta gcgaaagagg tctgcaacgc
     1081 aggagattcg ttcagaatgc tctgaatggt aacggagatc caaacaacat ggacaaagct
     1141 gtgaaactgt accgcaaact caaacgcgag attaccttcc acggagctaa agaaatctct
     1201 ctcagctatt ctgcaggtgc actcgcttct tgcatgggac tgatctacaa tagaatgggt
     1261 gcagttacaa cagaagttgc attcggtctg gtgtgtgcaa cctgcgaaca gattgccgac
     1321 agccagcatc gctctcatag acaactcgag tctagaggag gttctggtgg aaccggtgga
     1381 agtggaggga caggaggttc aggtggtact ggtggctctg gaggaacagg aggctccgga
     1441 ggtacaggag ggtcaggagg gactggaggc tctggaggta ctggcggttc aggaggtacc
     1501 atggtgtcca agagcgaaga agtgatcaag gagttcatga ggttcaaggt caggctggaa
     1561 ggaagcatga atggccacga gttcgagatt gagggagagg gtgaaggcag accttatgaa
     1621 ggaacacaga ctgctaaact caaagtgact aaaggtggtc ctctcccatt cgcttgggac
     1681 atcctgtctc cacagatcat gtatggcagc aaagcatacg tcaagcaccc agccgacgtg
     1741 ccagattaca tgaaactgag cttcccagag ggattcaaat gggaaagggt gatgcacttc
     1801 gaggatggag gactcgtcac agttacccaa gacacttctc tccaagacgg aaccctgatc
     1861 tacaaggtca agatgagagg cactaacttt ccacccgatg gacccgttat gcagaagaag
     1921 actctcggtt gggactatgc aactgagaga ctgtacccag aagacggagt gctcaagggt
     1981 gaactgctcg gtagactcaa gctcaaggac ggtggcctga acctggtgga gttcaagaca
     2041 atctacatgg ctaagaagcc agtccagctc cctggctact acttcgtgga caccaaactg
     2101 ggcattacat ctcacaatga agactacacc atcgtggagc agtacgaacg ctccgaaggt
     2161 aggcatcacc tgggacatgg aactggttcc aggtccatgg ctgatgaaga gaagctgcca
     2221 cctggatggg agaagagaat gtctcgcagc tctggcagag tttactactt caaccacatc
     2281 acaaacgcct cccagtggga aaggccatcc ggtaactctt cctccggtgg caagaacgga
     2341 caaggcgaac ctgctagagg aaccagtgct ggtggtagtg ctggtggtag tgctggtggt
     2401 agtgctggtg gtagtgctgg tggttccggc agtgctggtg gtagtgctgg tggtagtacc
     2461 agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg ctggtggtag tgctggtggt
     2521 tccggcagtg ctggtggtag tgctggtggt agtaccagtg ctggtggtag tgctggtggt
     2581 agtgctggtg gtagtgctgg tggtagtgct ggtggttccg gcagtgctgg tggtagtgct
     2641 ggtggtagta ccagtgctgg tggtagtgct ggtggtagtg ctggtggtag tgctggtggt
     2701 agtgctggtg gttctggtcc agatgtgcct cgcactcctg tggacaaggc taaactgagc
     2761 ttccagtttc caggtactgc tagcagcgaa gataacaaca tggctaccat caaggagttc
     2821 atgagattca aagttcgcat ggagggcagc atgaacggac acgagttcga aatcgagggt
     2881 gaaggtgagg gcagacctta cgaaggtact cagactgcaa agctgaaggt gaccaagggt
     2941 ggacctctgc catttgcctg ggacatcctc tctccacagt tcatgtacgg cagcgaggca
     3001 tacgtcaggc atcctgctga tatccctgat tacaagaaac tcccatttcc tgagggattc
     3061 aaatgggaga gagtcatgaa ctttgaagac ggtggtctgg tcactgtgac acaggactct
     3121 tctctgcagg acggaaccct catctgcaaa gttaagatga gaggaacaaa ctttcctcca
     3181 gacggacccg tgatgcagaa gaagactatg ggctgggagg cttctacaga gatgctgtac
     3241 cctgaggacg gagtgctgaa gggtcattcc taccaagctc tcaagctgaa ggacggtggc
     3301 cactatctcg tggagttcga gactatctac atggcaaaga aacccgtgca actgcctggt
     3361 gactactgtg ttgacacaaa gctggacatt acaagccaca acgaggatta caccatcgtt
     3421 gagcaatacg gaaggtctga gggcaggcat aggctcggca tggatgagag gtacaaggga
     3481 tccggttaca ctgacattga gatgaacaga ctcggtaagg gaggttctgg aggttctggt
     3541 ggtactggtg gttctggagg atctggtggt acaggaggta gcggaggttc tggtggaacc
     3601 tctgaagaac tgagagctgt cgcagacctg caaagactca acattgaact ggccaggaag
     3661 ctgctggaag ctgtggctag actgcaggaa ctcaacattg atctcgtgag gaagacctcc
     3721 gaactgaccg atgagaagac aatcagggag gagatcagaa aggtgaagga agagtccaag
     3781 aggattgtgg aggaagccga agaagaaatc agaagagcca aggaagaatc caggtacatc
     3841 gcagatgagt ccagaggaag ctaa
//
