LOCUS       sequence-311266-       11726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BRET biosensor for detection of G-alpha-i-GTP in lentiviral vector 
            pLVX-IRES-Hyg.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11726)
  AUTHORS   Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, 
            Garcia-Marcos M
  TITLE     Revealing the Activity of Trimeric G-proteins in Live Cells with a 
            Versatile Biosensor Design.
  JOURNAL   Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020.
  PUBMED    32634377
REFERENCE   2  (bases 1 to 11726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020."
COMMENT     Sequence Label: pLVX-IRES-G-alpha-i*-BERKY3
FEATURES             Location/Qualifiers
     source          1..11726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             696..1208
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             1233..1253
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1974..2225
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             2706..2957
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             3435..3686
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             3777..3806
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    join(3850..4424,4425..4427,4428..4436)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4017..4034)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4244..4263
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4462..5481
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             4462..5481
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    5495..6083
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5548..5568)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5966..5977)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6290..6923
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7051..7067)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7051..7067)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7064..7086)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7075..7091
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7099..7105,7106..7123,7124..7129))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7144..7165
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7282..7299)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7453..8041)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7533..7552)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8212..9003,9004..9072))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8835..8854
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9073..9177)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9225..9359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9262..9281)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9316..9335
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9527..10160
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10207..10332
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10829..11062
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11247..11291
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11554..11669
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tactagagga tctatttccg
      601 gtgaattcct cgaggctagc atgggatgta taaaatcaaa agggaaagac agcaagcttg
      661 gatcaggcgg tagcggggga tcaggcggta gcgggatggt cttcacactc gaagatttcg
      721 ttggggactg gcgacagaca gccggctaca acctggacca agtccttgaa cagggaggtg
      781 tgtccagttt gtttcagaat ctcggggtgt ccgtaactcc gatccaaagg attgtcctga
      841 gcggtgaaaa tgggctgaag atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg
      901 gcgaccaaat gggccagatc gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc
      961 actttaaggt gatcctgcac tatggcacac tggtaatcga cggggttacg ccgaacatga
     1021 tcgactattt cggacggccg tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg
     1081 taacagggac cctgtggaac ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg
     1141 gctccctgct gttccgagta accatcaacg gagtgaccgg ctggcggctg tgcgaacgca
     1201 ttctggcgga attcggatct ggtggttcag gcgaaaacct gtattttcag ggcggaagcg
     1261 gagaagagga ggaaaagaaa aaacaacagg aggaggaggc ggagaggctg aggaggatac
     1321 aagaggaaat ggaaaaagaa agaaagcggc gagaggaaga tgagcagcga cgccgcaagg
     1381 aggaggaaga gagaaggatg aaattggaga tggaggcaaa acgcaaacaa gaagaggaag
     1441 aacggaagaa acgggaggat gatgaaaagc ggaagaaaaa gggatctgga gggtcagggg
     1501 gcgcgcccat ggtttcaaag ggagaagagt tgttcacagg ggttgtaccg attcttgtag
     1561 aacttgacgg agatgtcaac gggcacaagt ttagtgtaag tggagaaggc gagggcgatg
     1621 cgacctatgg aaaactgacc cttaaattta tttgtactac gggtaaattg cccgttccct
     1681 ggccgacatt ggtaactaca ttcggatatg gtttgatgtg ctttgcaagg tatccggatc
     1741 acatgaagca gcatgatttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1801 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1861 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1921 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     1981 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2041 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2101 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     2161 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2221 acaagggcgc gcccggagga atggtttcaa agggagaaga gttgttcaca ggggttgtac
     2281 cgattcttgt agaacttgac ggagatgtca acgggcacaa gtttagtgta agtggagaag
     2341 gcgagggcga tgcgacctat ggaaaactga cccttaaatt tatttgtact acgggtaaat
     2401 tgcccgttcc ctggccgaca ttggtaacta cattcggata tggtttgatg tgctttgcaa
     2461 ggtatccgga tcacatgaag cagcatgatt tcttcaagtc cgccatgccc gaaggctacg
     2521 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     2581 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2641 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     2701 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     2761 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2821 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
     2881 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     2941 tggacgagct gtacaaggga tccggaggaa tggtttcaaa gggagaagag ttgttcacag
     3001 gggttgtacc gattcttgta gaacttgacg gagatgtcaa cgggcacaag tttagtgtaa
     3061 gtggagaagg cgagggcgat gcgacctatg gaaaactgac ccttaaattt atttgtacta
     3121 cgggtaaatt gcccgttccc tggccgacat tggtaactac attcggatat ggtttgatgt
     3181 gctttgcaag gtatccggat cacatgaagc agcatgattt cttcaagtcc gccatgcccg
     3241 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     3301 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     3361 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     3421 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     3481 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     3541 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag
     3601 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     3661 ctctcggcat ggacgagctg tacaagggat ccggtggcac cggtggatca ggctcttctc
     3721 gaggttacta ccatggtatt tgggtgggtg aagaaggtcg actttctcga ggtggagaac
     3781 aaaaacttat ttctgaagaa gatctgtgat ctagactcga gactagttct agagcggccg
     3841 cggatcccgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     3901 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     3961 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     4021 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     4081 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     4141 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     4201 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     4261 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     4321 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     4381 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccgta
     4441 ccaaagatgg atagatccgg aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     4501 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     4561 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     4621 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     4681 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     4741 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     4801 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     4861 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     4921 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     4981 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     5041 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     5101 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     5161 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     5221 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     5281 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     5341 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     5401 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     5461 cgtccgaggg caaaggaata gacgcgtctg gaacaatcaa cctctggatt acaaaatttg
     5521 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     5581 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     5641 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     5701 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     5761 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     5821 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     5881 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     5941 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     6001 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     6061 ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat
     6121 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     6181 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     6241 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta
     6301 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     6361 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     6421 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     6481 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     6541 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     6601 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     6661 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     6721 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6781 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6841 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6901 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     6961 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc
     7021 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7081 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7141 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7201 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7261 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7321 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7381 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7441 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7501 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7561 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7621 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7681 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7741 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7801 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7861 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7921 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7981 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8041 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8101 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8161 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8221 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8281 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8341 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8401 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8461 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8521 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8581 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8641 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8701 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8761 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8821 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8881 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8941 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9001 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9061 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9121 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9181 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag
     9241 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9301 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
     9361 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
     9421 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     9481 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
     9541 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     9601 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     9661 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     9721 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     9781 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     9841 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     9901 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     9961 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
    10021 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    10081 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    10141 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
    10201 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
    10261 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
    10321 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
    10381 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
    10441 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
    10501 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
    10561 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
    10621 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
    10681 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
    10741 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
    10801 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
    10861 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
    10921 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
    10981 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
    11041 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
    11101 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
    11161 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
    11221 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
    11281 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
    11341 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
    11401 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
    11461 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
    11521 ttcgattagt gaacggatct cgacggtatc gcctttaaaa gaaaaggggg gattgggggg
    11581 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
    11641 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
    11701 gtttatcgat aagcttggga gttccg
//
