LOCUS       sequence-311268-        2106 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Signaling reporter island (SiRI) construct for spatially multiplexed
            imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium 
            indicator), Xpress tag, and S1 protein scaffold..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Linghu C, Johnson SL, Valdes PA, Shemesh OA, Park WM, Park D, 
            Piatkevich KD, Wassie AT, Liu Y, An B, Barnes SA, Celiker OT, Yao 
            CC, Yu CJ, Wang R, Adamala KP, Bear MF, Keating AE, Boyden ES
  TITLE     Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling 
            Network Dynamics.
  JOURNAL   Cell. 2020 Nov 17. pii: S0092-8674(20)31399-4. doi: 
            10.1016/j.cell.2020.10.035.
  PUBMED    33232692
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Nov 17. pii: S0092-8674(20)31399-4. doi: 
            10.1016/j.cell.2020.10.035."
COMMENT     Sequence Label: pAAV-UBC-S1-jRCaMP1a
FEATURES             Location/Qualifiers
     source          1..2106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             136..153
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             157..189
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(193..201,202..216)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             244..300
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     CDS             1671..1685
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
ORIGIN
        1 atggcagaag cagaaagcgc actggagtat gcacaacaag cactggagaa ggctcagctg
       61 gcactccaag ctgcaagaca ggctctcaag gctggaggat ccggtggaac cggtggaagt
      121 ggagggacag gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag
      181 caaatgggtc gggatctgta cgacgatgac gataaggatc tcgcaacaat ggtcgactca
      241 tcacgtcgta agtggaataa gacaggtcac gcagtcagag ctataggtcg gctgagctca
      301 gcgatcaact gcgaaatgat gtacccagcg gatggtggtc tgcgtggtta cactcacatg
      361 gcgctgaaag ttgatggcgg cggtcacctg tcctgttctt tcgtgaccac ctaccgctcc
      421 aaaaagactg tcggcaacat taagatgcct gccattcata gcgtcagcca ccgtctggag
      481 cgcctggagg agagcgataa cgaaatgttt gtcgtacagc gtgaacacgc agttgccaag
      541 tttgtgggcc tgggtggtgg cggcggtacc ggagggagca tgaactccct gatcaaggag
      601 aacatgcgta tgaaagtggt tctggaaggc tccgtaaacg gccaccagtt caaatgcact
      661 ggtgaaggcg aaggcaaccc gtatatgggc acccagacta tgcgtatcaa agtgatcgag
      721 ggtggtccgc tgccgtttgc gttcgacatc ctggcgacgt cctttatgta tggctcccgt
      781 accttcatca aatatccgaa aggcatcccg gatttcttta agcagtcctt cccggaaggt
      841 tttacctggg aacgtgtgac ccgttacgaa gacggcggcg taattaccgt tatgcaagac
      901 acgtctctgg aggatggctg cctggtgtat cacgtgcagg ttcgcggtgt gaacttcccg
      961 agcaatggtg ctgtaatgca aaagaaaacc aaaggttggg agcctacgga ctcccaaacc
     1021 actgaagagc agatcgcaga atttaaagag gctttctccc tatttgacaa ggacggggat
     1081 gggacaataa caaccaagga gctggggacg gtgatgcggt ctctggggca gaaccccaca
     1141 gaagcagagc tgcaggacat gatcaatgaa gtagatgccg acggtgacgg cacaatcgac
     1201 ttccctgagt tcctgattat gatggcaaga aaaatgaaat acacagacag tgaagaagaa
     1261 attagagaag cgttcggcgt gtttgataag gatggcaatg gctacatcag tgcagcagag
     1321 cttcgccacg tgatgacaaa ccttggagag aagttaacag atgaagaggt tgatgaaatg
     1381 atcagggaag cagacagcga tggggatggt caggtaaact acgaagagtt tgtacaaatg
     1441 atgacagcga agggaggttc aggtggaacc ggtggaagtg gaggtaccat gaagatggag
     1501 gagctgttca agaagcataa gattgtggct gtgctgagag ctaactctgt ggaggaagct
     1561 aagaagaagg ctctggctgt gttcctggga ggcgttcacc tgatcgagat tactttcaca
     1621 gtcccagatg ccgataccgt gatcaaggaa ctgtctttcc tgaaagaaat gggagccatc
     1681 attggagctg gcaccgtcac ctccgtcgaa caatgtagga aagcagttga gtccggtgct
     1741 gagttcatcg tctctccaca cctggacgag gagatttctc agttctgcaa agagaaaggt
     1801 gtgttctaca tgcctggcgt catgacacct actgaactcg tcaaagccat gaagctcgga
     1861 cataccatcc tgaagctgtt cccaggagaa gtggtgggac cacagttcgt gaaagccatg
     1921 aaaggaccct tccctaatgt gaagtttgtg cccactggtg gtgtgaatct ggataacgtg
     1981 tgtgagtggt tcaaggcagg cgttctcgct gtcggagttg gttctgcact cgtgaagggt
     2041 acacctgtgg aagtcgctga gaaggcaaag gctttcgttg agaagattag aggatgtact
     2101 gagtaa
//
