LOCUS       sequence-311269-       12091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BRET biosensor for detection of G-alpha-q-GTP in lentiviral vector 
            pLVX-IRES-Hyg.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12091)
  AUTHORS   Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, 
            Garcia-Marcos M
  TITLE     Revealing the Activity of Trimeric G-proteins in Live Cells with a 
            Versatile Biosensor Design.
  JOURNAL   Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020.
  PUBMED    32634377
REFERENCE   2  (bases 1 to 12091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020."
COMMENT     Sequence Label: pLVX-IRES-G-alpha-q*-BERKY3
FEATURES             Location/Qualifiers
     source          1..12091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             696..1208
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             1233..1253
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1974..2225
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             2706..2957
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             3435..3686
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             4142..4171
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    join(4215..4789,4790..4792,4793..4801)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4382..4399)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4609..4628
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4827..5846
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             4827..5846
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    5860..6448
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5913..5933)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6331..6342)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6655..7288
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7416..7432)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7416..7432)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7429..7451)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7440..7456
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7464..7470,7471..7488,7489..7494))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7509..7530
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7647..7664)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7818..8406)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7898..7917)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8577..9368,9369..9437))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9200..9219
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9438..9542)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9590..9724
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9627..9646)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9681..9700
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9892..10525
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10572..10697
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11194..11427
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11612..11656
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11919..12034
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tactagagga tctatttccg
      601 gtgaattcct cgaggctagc atgggatgta taaaatcaaa agggaaagac agcaagcttg
      661 gatcaggcgg tagcggggga tcaggcggta gcgggatggt cttcacactc gaagatttcg
      721 ttggggactg gcgacagaca gccggctaca acctggacca agtccttgaa cagggaggtg
      781 tgtccagttt gtttcagaat ctcggggtgt ccgtaactcc gatccaaagg attgtcctga
      841 gcggtgaaaa tgggctgaag atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg
      901 gcgaccaaat gggccagatc gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc
      961 actttaaggt gatcctgcac tatggcacac tggtaatcga cggggttacg ccgaacatga
     1021 tcgactattt cggacggccg tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg
     1081 taacagggac cctgtggaac ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg
     1141 gctccctgct gttccgagta accatcaacg gagtgaccgg ctggcggctg tgcgaacgca
     1201 ttctggcgga attcggatct ggtggttcag gcgaaaacct gtattttcag ggcggaagcg
     1261 gagaagagga ggaaaagaaa aaacaacagg aggaggaggc ggagaggctg aggaggatac
     1321 aagaggaaat ggaaaaagaa agaaagcggc gagaggaaga tgagcagcga cgccgcaagg
     1381 aggaggaaga gagaaggatg aaattggaga tggaggcaaa acgcaaacaa gaagaggaag
     1441 aacggaagaa acgggaggat gatgaaaagc ggaagaaaaa gggatctgga gggtcagggg
     1501 gcgcgcccat ggtttcaaag ggagaagagt tgttcacagg ggttgtaccg attcttgtag
     1561 aacttgacgg agatgtcaac gggcacaagt ttagtgtaag tggagaaggc gagggcgatg
     1621 cgacctatgg aaaactgacc cttaaattta tttgtactac gggtaaattg cccgttccct
     1681 ggccgacatt ggtaactaca ttcggatatg gtttgatgtg ctttgcaagg tatccggatc
     1741 acatgaagca gcatgatttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1801 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1861 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1921 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     1981 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2041 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2101 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     2161 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2221 acaagggcgc gcccggagga atggtttcaa agggagaaga gttgttcaca ggggttgtac
     2281 cgattcttgt agaacttgac ggagatgtca acgggcacaa gtttagtgta agtggagaag
     2341 gcgagggcga tgcgacctat ggaaaactga cccttaaatt tatttgtact acgggtaaat
     2401 tgcccgttcc ctggccgaca ttggtaacta cattcggata tggtttgatg tgctttgcaa
     2461 ggtatccgga tcacatgaag cagcatgatt tcttcaagtc cgccatgccc gaaggctacg
     2521 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     2581 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2641 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     2701 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     2761 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2821 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
     2881 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     2941 tggacgagct gtacaaggga tccggaggaa tggtttcaaa gggagaagag ttgttcacag
     3001 gggttgtacc gattcttgta gaacttgacg gagatgtcaa cgggcacaag tttagtgtaa
     3061 gtggagaagg cgagggcgat gcgacctatg gaaaactgac ccttaaattt atttgtacta
     3121 cgggtaaatt gcccgttccc tggccgacat tggtaactac attcggatat ggtttgatgt
     3181 gctttgcaag gtatccggat cacatgaagc agcatgattt cttcaagtcc gccatgcccg
     3241 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     3301 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     3361 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     3421 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     3481 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     3541 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag
     3601 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     3661 ctctcggcat ggacgagctg tacaagggat ccggtggcac cggtggatca ggctcttctc
     3721 gagagaagta cctggaggac cggggcgagg tgacttttga gaagatcttc tcccagaagc
     3781 tggggtacct gcttttccga gacttctgcc tgaagcacct ggaggaggcc aagcccttgg
     3841 tagagttcta cgaggagatc aagaaatacg agaagctgga gacagaggag gagcgcctgg
     3901 tctgcagccg agagatcttc gacacgtaca tcatgaagga gctgctggcc tgctcacatc
     3961 ctttctcgaa gagcgccatt gagcacgtcc agggccatct ggtgaagaag caggtgcctc
     4021 cggatctctt ccagccatat attgaagaaa tttgccagaa cctccgagga gacgtgttcc
     4081 agaaattcat cgagagcgat aaattcacac ggttttgcca gtggaagtga ctcgaggtgg
     4141 agaacaaaaa cttatttctg aagaagatct gtgatctaga ctcgagacta gttctagagc
     4201 ggccgcggat cccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
     4261 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     4321 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     4381 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     4441 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     4501 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     4561 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     4621 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     4681 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     4741 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac
     4801 ccgtaccaaa gatggataga tccggaaagc ctgaactcac cgcgacgtct gtcgagaagt
     4861 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     4921 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     4981 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     5041 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     5101 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     5161 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     5221 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     5281 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     5341 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     5401 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     5461 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     5521 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     5581 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     5641 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     5701 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     5761 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     5821 gcactcgtcc gagggcaaag gaatagacgc gtctggaaca atcaacctct ggattacaaa
     5881 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     5941 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6001 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6061 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6121 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6181 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     6241 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     6301 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6361 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     6421 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa
     6481 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     6541 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     6601 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag
     6661 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     6721 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     6781 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     6841 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     6901 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     6961 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     7021 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     7081 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     7141 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7201 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7261 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7321 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgttta
     7381 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7441 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7501 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7561 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7621 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7681 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7741 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7801 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7861 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7921 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7981 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8041 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8101 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8161 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8221 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8281 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8341 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8401 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8461 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8521 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8581 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8641 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8701 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8761 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8821 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8881 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8941 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9001 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9061 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9121 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9181 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9241 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9301 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9361 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9421 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9481 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9541 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat
     9601 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9661 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9721 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
     9781 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
     9841 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc
     9901 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     9961 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
    10021 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
    10081 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
    10141 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
    10201 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
    10261 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
    10321 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
    10381 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
    10441 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
    10501 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
    10561 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
    10621 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
    10681 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
    10741 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
    10801 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
    10861 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
    10921 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
    10981 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
    11041 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
    11101 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
    11161 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
    11221 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
    11281 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
    11341 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
    11401 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
    11461 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
    11521 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
    11581 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
    11641 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
    11701 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
    11761 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
    11821 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
    11881 atccattcga ttagtgaacg gatctcgacg gtatcgcctt taaaagaaaa ggggggattg
    11941 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
    12001 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
    12061 atccagttta tcgataagct tgggagttcc g
//
