LOCUS       sequence-311270-       12238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BRET biosensor for detection of free G-beta-gamma in lentiviral 
            vector pLVX-IRES-Hyg.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12238)
  AUTHORS   Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, 
            Garcia-Marcos M
  TITLE     Revealing the Activity of Trimeric G-proteins in Live Cells with a 
            Versatile Biosensor Design.
  JOURNAL   Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020.
  PUBMED    32634377
REFERENCE   2  (bases 1 to 12238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020."
COMMENT     Sequence Label: pLVX-IRES-G-beta-gamma-BERKY3
FEATURES             Location/Qualifiers
     source          1..12238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             696..1208
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             1233..1253
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1974..2225
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             2706..2957
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             3435..3686
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             4289..4318
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    join(4362..4936,4937..4939,4940..4948)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4529..4546)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4756..4775
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4974..5993
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             4974..5993
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    6007..6595
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6060..6080)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6478..6489)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6802..7435
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7563..7579)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7563..7579)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7576..7598)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7587..7603
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7611..7617,7618..7635,7636..7641))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7656..7677
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7794..7811)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7965..8553)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8045..8064)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8724..9515,9516..9584))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9347..9366
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9585..9689)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9737..9871
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9774..9793)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9828..9847
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10039..10672
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10719..10844
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11341..11574
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11759..11803
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12066..12181
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tactagagga tctatttccg
      601 gtgaattcct cgaggctagc atgggatgta taaaatcaaa agggaaagac agcaagcttg
      661 gatcaggcgg tagcggggga tcaggcggta gcgggatggt cttcacactc gaagatttcg
      721 ttggggactg gcgacagaca gccggctaca acctggacca agtccttgaa cagggaggtg
      781 tgtccagttt gtttcagaat ctcggggtgt ccgtaactcc gatccaaagg attgtcctga
      841 gcggtgaaaa tgggctgaag atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg
      901 gcgaccaaat gggccagatc gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc
      961 actttaaggt gatcctgcac tatggcacac tggtaatcga cggggttacg ccgaacatga
     1021 tcgactattt cggacggccg tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg
     1081 taacagggac cctgtggaac ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg
     1141 gctccctgct gttccgagta accatcaacg gagtgaccgg ctggcggctg tgcgaacgca
     1201 ttctggcgga attcggatct ggtggttcag gcgaaaacct gtattttcag ggcggaagcg
     1261 gagaagagga ggaaaagaaa aaacaacagg aggaggaggc ggagaggctg aggaggatac
     1321 aagaggaaat ggaaaaagaa agaaagcggc gagaggaaga tgagcagcga cgccgcaagg
     1381 aggaggaaga gagaaggatg aaattggaga tggaggcaaa acgcaaacaa gaagaggaag
     1441 aacggaagaa acgggaggat gatgaaaagc ggaagaaaaa gggatctgga gggtcagggg
     1501 gcgcgcccat ggtttcaaag ggagaagagt tgttcacagg ggttgtaccg attcttgtag
     1561 aacttgacgg agatgtcaac gggcacaagt ttagtgtaag tggagaaggc gagggcgatg
     1621 cgacctatgg aaaactgacc cttaaattta tttgtactac gggtaaattg cccgttccct
     1681 ggccgacatt ggtaactaca ttcggatatg gtttgatgtg ctttgcaagg tatccggatc
     1741 acatgaagca gcatgatttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1801 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1861 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1921 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     1981 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2041 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2101 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     2161 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2221 acaagggcgc gcccggagga atggtttcaa agggagaaga gttgttcaca ggggttgtac
     2281 cgattcttgt agaacttgac ggagatgtca acgggcacaa gtttagtgta agtggagaag
     2341 gcgagggcga tgcgacctat ggaaaactga cccttaaatt tatttgtact acgggtaaat
     2401 tgcccgttcc ctggccgaca ttggtaacta cattcggata tggtttgatg tgctttgcaa
     2461 ggtatccgga tcacatgaag cagcatgatt tcttcaagtc cgccatgccc gaaggctacg
     2521 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     2581 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2641 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     2701 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     2761 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2821 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
     2881 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     2941 tggacgagct gtacaaggga tccggaggaa tggtttcaaa gggagaagag ttgttcacag
     3001 gggttgtacc gattcttgta gaacttgacg gagatgtcaa cgggcacaag tttagtgtaa
     3061 gtggagaagg cgagggcgat gcgacctatg gaaaactgac ccttaaattt atttgtacta
     3121 cgggtaaatt gcccgttccc tggccgacat tggtaactac attcggatat ggtttgatgt
     3181 gctttgcaag gtatccggat cacatgaagc agcatgattt cttcaagtcc gccatgcccg
     3241 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     3301 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     3361 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     3421 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     3481 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     3541 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag
     3601 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     3661 ctctcggcat ggacgagctg tacaagggat ccggtggcac cggtggatca ggctcttctc
     3721 gagagaactt ccctctggtg atctctgagc gctggcagca ggaagtggcg gaaacagttt
     3781 atgaagcagt aaatgcagac acggataaaa tcgaggccag gaagagagct aaaaataagc
     3841 agcttggcca cgaagaagat tacgccctgg gaagagactg catcgtgcac gggtacatgc
     3901 tgaagctggg gaaccctttc ctgacccagt ggcagcgccg ctatttttac ctctttccga
     3961 acagacttga gtggagagga gaaggcgagt cgcgacaaag tttactgaca atggaacaga
     4021 ttgtgtccgt ggaagaaact cagattaaag acaaaaagtg cattttgttg agaataaaag
     4081 gagggaagca gttcgttttg cagtgtgaga gtgacccaga gtttgtgcag tggaagaaag
     4141 agctgacgga gacattcatg gaggcccagc ggctgctacg gcgagccccc aagttcctca
     4201 acaaatcccg ctcagccgtc gtggaactct caaagcctcc cctctgccat aggaacagca
     4261 acggcctcgg aggttgactc gaggtggaga acaaaaactt atttctgaag aagatctgtg
     4321 atctagactc gagactagtt ctagagcggc cgcggatccc gcccctctcc ctcccccccc
     4381 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     4441 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     4501 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     4561 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     4621 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     4681 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     4741 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     4801 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     4861 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     4921 aaaacacgat gataagcttg ccacaacccg taccaaagat ggatagatcc ggaaagcctg
     4981 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
     5041 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
     5101 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
     5161 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
     5221 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
     5281 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
     5341 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
     5401 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
     5461 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
     5521 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
     5581 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
     5641 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
     5701 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
     5761 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
     5821 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
     5881 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
     5941 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagacgcgtc
     6001 tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6061 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6121 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6181 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6241 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6301 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6361 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     6421 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     6481 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     6541 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
     6601 attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     6661 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     6721 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     6781 ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     6841 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     6901 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     6961 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     7021 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     7081 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     7141 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     7201 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     7261 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7321 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7381 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7441 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7501 gagaggcctt gacattgcta gcgtttaccg tcgacctcta gctagagctt ggcgtaatca
     7561 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     7621 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     7681 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7741 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7801 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7861 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7921 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7981 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8041 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8101 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8161 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8221 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8281 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8341 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8401 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8461 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8521 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8581 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8641 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8701 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8761 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8821 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8881 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8941 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9001 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9061 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9121 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9181 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9241 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9301 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9361 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9421 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9481 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9541 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9601 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9661 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     9721 gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     9781 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     9841 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
     9901 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
     9961 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
    10021 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
    10081 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
    10141 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
    10201 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
    10261 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
    10321 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
    10381 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
    10441 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
    10501 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
    10561 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
    10621 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
    10681 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
    10741 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
    10801 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
    10861 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
    10921 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
    10981 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
    11041 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
    11101 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
    11161 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
    11221 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
    11281 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
    11341 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
    11401 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
    11461 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
    11521 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
    11581 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
    11641 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
    11701 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
    11761 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
    11821 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
    11881 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
    11941 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
    12001 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
    12061 tcgcctttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
    12121 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
    12181 ttcgggttta ttacagggac agcagagatc cagtttatcg ataagcttgg gagttccg
//
