LOCUS       sequence-311271-       10755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant mitoTALEN vector assembly, Destination vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10755)
  AUTHORS   Kazama T, Okuno M, Watari Y, Yanase S, Koizuka C, Tsuruta Y, Sugaya 
            H, Toyoda A, Itoh T, Tsutsumi N, Toriyama K, Koizuka N, Arimura SI
  TITLE     Curing cytoplasmic male sterility via TALEN-mediated mitochondrial 
            genome editing.
  JOURNAL   Nat Plants. 2019 Jul;5(7):722-730. doi: 10.1038/s41477-019-0459-z. 
            Epub 2019 Jul 8.
  PUBMED    31285556
REFERENCE   2  (bases 1 to 10755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-019-0459-z"; journalName: "Nat Plants"; date: 
            "2019-07"; volume: "5"; issue: "7"; pages: "722-730"
COMMENT     Sequence Label: pDESTpK7WG2mito
FEATURES             Location/Qualifiers
     source          1..10755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    366..421
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     protein_bind    618..742
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(767..772,773..790,791..797)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             851..1510
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(917..936)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1852..2157
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2036..2055
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2198..2322)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2694..2873
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2907..3701
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2961..2980)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3571..3590
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      3741..3993
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4086..4110
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4631..5422
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5668..6256
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6157..6176
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6410..6427
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6442..6582
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6508..6527)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6668..6690
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6926..7120
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7186..8259)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8688..9317)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10648..10672
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ctctaccgac agtggtccca aagatggacc
      181 cccacccacg aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacaggct tcttgagatc
      361 cttcaacaat taccaacaac aacaaacaac aaacaacatt acaattacta tttacaatta
      421 cagtcgaccc acgcgtccga taatttcccc aatttctcag agagactcaa aatcaccacc
      481 accaccaaat cgccgccatg tttaaacaag cttctcgtct cctctcccga tctgtcgccg
      541 ccgcatcttc caaatcggtg acgactcgtg ccttttcaac ggaacttcca tcgacgctcg
      601 attccgctag tgatatcaca agtttgtaca aaaaagctga acgagaaacg taaaatgata
      661 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
      721 cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc
      781 ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa
      841 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
      901 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
      961 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     1021 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     1081 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     1141 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     1201 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     1261 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     1321 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     1381 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1441 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     1501 cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
     1561 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
     1621 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
     1681 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
     1741 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
     1801 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt
     1861 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
     1921 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
     1981 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
     2041 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
     2101 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
     2161 tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt
     2221 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
     2281 atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatatccc gcggccatgc
     2341 tagagtccgc aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca
     2401 gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat
     2461 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     2521 aaaatttcta attcctaaaa ccaaaatcca gtgacctgca ggcatgcgac gtcgggccct
     2581 ctagaggatc cccgggtacc gcgaattatc gatcatgagc ggagaattaa gggagtcacg
     2641 ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg acagaaccgc
     2701 aacgttgaag gagccactga gccgcgggtt tctggagttt aatgagctaa gcacatacgt
     2761 cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt
     2821 cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc
     2881 atattcactc tcaactcgat cgaggcatga ttgaacaaga tggattgcac gcaggttctc
     2941 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     3001 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     3061 acctgtccgg tgccctgaat gaactccaag acgaggcagc gcggctatcg tggctggcca
     3121 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     3181 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     3241 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     3301 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     3361 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     3421 ccaggctcaa ggcgcggatg cccgacggcg aggatctcgt cgtgacccac ggcgatgcct
     3481 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     3541 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     3601 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     3661 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgg
     3721 actctagcta gagtcaagca gatcgttcaa acatttggca ataaagtttc ttaagattga
     3781 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     3841 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     3901 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     3961 tatcgcgcgc ggtgtcatct atgttactag atcgaccggc atgcaagctg ataattcaat
     4021 tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
     4081 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
     4141 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg
     4201 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
     4261 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
     4321 gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga cataagcctg
     4381 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     4441 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     4501 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     4561 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     4621 gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     4681 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     4741 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     4801 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     4861 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     4921 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     4981 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     5041 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     5101 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     5161 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     5221 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     5281 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     5341 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     5401 caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg
     5461 cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta
     5521 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     5581 agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5641 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5701 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5761 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     5821 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5881 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5941 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6001 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6061 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6121 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6181 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6241 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6301 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6361 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6421 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     6481 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     6541 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     6601 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     6661 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     6721 cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc
     6781 tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg
     6841 acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc
     6901 ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat
     6961 aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta
     7021 catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc
     7081 aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc
     7141 tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga
     7201 tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc
     7261 ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa
     7321 acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca
     7381 tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc
     7441 gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt
     7501 gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc
     7561 gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg
     7621 gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca
     7681 ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac
     7741 gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg
     7801 gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact
     7861 ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat
     7921 cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct
     7981 gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc
     8041 gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt
     8101 gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg
     8161 ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc
     8221 gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg
     8281 gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc
     8341 gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg
     8401 ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg
     8461 tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta
     8521 catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg
     8581 cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc
     8641 acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt
     8701 gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat
     8761 tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg
     8821 gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc
     8881 gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca
     8941 atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc
     9001 tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc
     9061 tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca
     9121 atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa
     9181 tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc
     9241 agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt
     9301 acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat
     9361 acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc
     9421 ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca
     9481 aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt
     9541 acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc
     9601 cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc
     9661 gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt
     9721 cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc
     9781 agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg
     9841 tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg
     9901 cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac
     9961 ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg
    10021 caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg
    10081 ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat
    10141 cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt
    10201 gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac
    10261 gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt
    10321 ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa
    10381 gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga
    10441 cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt
    10501 actttaaagt actttaaagt actttaaagt actttgatcc cgaggggaac cctgtggttg
    10561 gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt
    10621 attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga
    10681 tagtttaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctaa gctcctcaga
    10741 agaccagagg gctat
//
