LOCUS       sequence-311273-       10210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BRET biosensor for detection of G-alpha-i-GTP in lentiviral vector 
            pLenti-hSyn.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, 
            Garcia-Marcos M
  TITLE     Revealing the Activity of Trimeric G-proteins in Live Cells with a 
            Versatile Biosensor Design.
  JOURNAL   Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020.
  PUBMED    32634377
REFERENCE   2  (bases 1 to 10210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020."
COMMENT     Sequence Label: pLenti-hSyn-G-alpha-i*-BERKY1
FEATURES             Location/Qualifiers
     source          1..10210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..448
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      507..516
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             588..1100
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             1125..1145
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(1707..1726)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1866..2117
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     2054..2075
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2208..2237
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    2271..2859
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2324..2344)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2742..2753)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2862..2878)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2863..2879)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3384..3564
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3590..3607)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3596..3820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3866..4294
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3953..3972)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4163..4184
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4303..4323)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4308..4637
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4488..4623
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4550..4569
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4685..4732
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4751..5125
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5255..5376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5292..5311)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5346..5365
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5425..5441)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5425..5441)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5438..5460)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5449..5465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5473..5479,5480..5497,5498..5503))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5518..5539
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5656..5673)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5827..6415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5907..5926)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6586..7377,7378..7446))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7209..7228
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7447..7551)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7626..7645)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7817..8196
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8198..8396
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8351..8371
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8414..8594
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8641..8766
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9259..9492
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9677..9721
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10019..10136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
       61 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      121 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      181 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      241 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      301 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      361 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      421 aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg ctagaggatc cttcgaaacc
      481 ggctagagga tccttcgaaa gctagcgcca ccatgggatg tataaaatca aaagggaaag
      541 acagcaagct tggatcaggc ggtagcgggg gatcaggcgg tagcgggatg gtcttcacac
      601 tcgaagattt cgttggggac tggcgacaga cagccggcta caacctggac caagtccttg
      661 aacagggagg tgtgtccagt ttgtttcaga atctcggggt gtccgtaact ccgatccaaa
      721 ggattgtcct gagcggtgaa aatgggctga agatcgacat ccatgtcatc atcccgtatg
      781 aaggtctgag cggcgaccaa atgggccaga tcgaaaaaat ttttaaggtg gtgtaccctg
      841 tggatgatca tcactttaag gtgatcctgc actatggcac actggtaatc gacggggtta
      901 cgccgaacat gatcgactat ttcggacggc cgtatgaagg catcgccgtg ttcgacggca
      961 aaaagatcac tgtaacaggg accctgtgga acggcaacaa aattatcgac gagcgcctga
     1021 tcaaccccga cggctccctg ctgttccgag taaccatcaa cggagtgacc ggctggcggc
     1081 tgtgcgaacg cattctggcg gaattcggat ctggtggttc aggcgaaaac ctgtattttc
     1141 agggcggaag cggagaagag gaggaaaaga aaaaacaaca ggaggaggag gcggagaggc
     1201 tgaggaggat acaagaggaa atggaaaaag aaagaaagcg gcgagaggaa gatgagcagc
     1261 gacgccgcaa ggaggaggaa gagagaagga tgaaattgga gatggaggca aaacgcaaac
     1321 aagaagagga agaacggaag aaacgggagg atgatgaaaa gcggaagaaa aagggatctg
     1381 gagggtcagg gggcgcgccc atggtttcaa agggagaaga gttgttcaca ggggttgtac
     1441 cgattcttgt agaacttgac ggagatgtca acgggcacaa gtttagtgta agtggagaag
     1501 gcgagggcga tgcgacctat ggaaaactga cccttaaatt tatttgtact acgggtaaat
     1561 tgcccgttcc ctggccgaca ttggtaacta cattcggata tggtttgatg tgctttgcaa
     1621 ggtatccgga tcacatgaag cagcatgatt tcttcaagtc cgccatgccc gaaggctacg
     1681 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1741 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     1801 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     1861 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     1921 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     1981 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
     2041 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     2101 tggacgagct gtacaaggga tccggtggca ccggtggatc aggctcttct cgaggttact
     2161 accatggtat ttgggtgggt gaagaaggtc gactttctcg aggtggagaa caaaaactta
     2221 tttctgaaga agatctgtga tctagaccgg tcgatatcaa gcttatcgat aatcaacctc
     2281 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     2341 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     2401 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     2461 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     2521 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     2581 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     2641 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     2701 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     2761 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     2821 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
     2881 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     2941 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     3001 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     3061 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     3121 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     3181 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     3241 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     3301 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     3361 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     3421 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     3481 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     3541 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
     3601 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     3661 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     3721 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     3781 gacaatagca ggcatgctgg ggatgcggcg ggctctatgg cttctgaggc ggaaagaacc
     3841 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3901 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3961 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4021 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4081 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4141 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4201 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4261 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     4321 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4381 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4441 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4501 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4561 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4621 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     4681 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     4741 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     4801 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     4861 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     4921 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     4981 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     5041 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     5101 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     5161 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     5221 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     5281 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5341 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     5401 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     5461 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5521 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5581 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5641 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5701 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5761 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5821 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5881 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5941 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6001 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6061 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6121 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6181 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6241 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     6301 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6361 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6421 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6481 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6541 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6601 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6661 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6721 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6781 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6841 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6901 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6961 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7021 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7081 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7141 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7201 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7261 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7321 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7381 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7441 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7501 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7561 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     7621 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     7681 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     7741 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     7801 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     7861 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     7921 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     7981 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     8041 cacttggcag tacatcaagt gtatcatatg ccaagcacgc cccctattga cgtcaatgac
     8101 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     8161 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     8221 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     8281 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     8341 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
     8401 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8461 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8521 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8581 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     8641 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     8701 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     8761 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     8821 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     8881 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     8941 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     9001 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     9061 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     9121 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     9181 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     9241 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     9301 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     9361 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     9421 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     9481 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     9541 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     9601 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     9661 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     9721 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     9781 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     9841 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     9901 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     9961 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
    10021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
    10081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
    10141 tttattacag ggacagcaga gatccagttt ggttaattaa gtgtctagac tgcagagggc
    10201 cctgcgtatg
//
