LOCUS       sequence-311275-       11649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BRET biosensor for detection of G-alpha-i-GTP in lentiviral vector 
            pLenti-hSyn.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11649)
  AUTHORS   Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, 
            Garcia-Marcos M
  TITLE     Revealing the Activity of Trimeric G-proteins in Live Cells with a 
            Versatile Biosensor Design.
  JOURNAL   Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020.
  PUBMED    32634377
REFERENCE   2  (bases 1 to 11649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020."
COMMENT     Sequence Label: pLenti-hSyn-G-alpha-i*-BERKY3
FEATURES             Location/Qualifiers
     source          1..11649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..448
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      485..494
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             566..1078
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             1103..1123
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1844..2095
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             2576..2827
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             3305..3556
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             3647..3676
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    3710..4298
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3763..3783)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4181..4192)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4301..4317)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4302..4318)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4823..5003
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5029..5046)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5035..5259
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5305..5733
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5392..5411)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5602..5623
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5742..5762)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5747..6076
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5927..6062
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5989..6008
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6124..6171
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6190..6564
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6694..6815
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6731..6750)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6785..6804
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6864..6880)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6864..6880)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6877..6899)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6888..6904
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6912..6918,6919..6936,6937..6942))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6957..6978
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7095..7112)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7266..7854)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7346..7365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8025..8816,8817..8885))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8648..8667
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8886..8990)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9065..9084)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9256..9635
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9637..9835
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9790..9810
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9853..10033
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10080..10205
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10698..10931
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11116..11160
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11458..11575
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
       61 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      121 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      181 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      241 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      301 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      361 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      421 aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg ctagaggatc cttcgaaagc
      481 tagcgccacc atgggatgta taaaatcaaa agggaaagac agcaagcttg gatcaggcgg
      541 tagcggggga tcaggcggta gcgggatggt cttcacactc gaagatttcg ttggggactg
      601 gcgacagaca gccggctaca acctggacca agtccttgaa cagggaggtg tgtccagttt
      661 gtttcagaat ctcggggtgt ccgtaactcc gatccaaagg attgtcctga gcggtgaaaa
      721 tgggctgaag atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg gcgaccaaat
      781 gggccagatc gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc actttaaggt
      841 gatcctgcac tatggcacac tggtaatcga cggggttacg ccgaacatga tcgactattt
      901 cggacggccg tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg taacagggac
      961 cctgtggaac ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg gctccctgct
     1021 gttccgagta accatcaacg gagtgaccgg ctggcggctg tgcgaacgca ttctggcgga
     1081 attcggatct ggtggttcag gcgaaaacct gtattttcag ggcggaagcg gagaagagga
     1141 ggaaaagaaa aaacaacagg aggaggaggc ggagaggctg aggaggatac aagaggaaat
     1201 ggaaaaagaa agaaagcggc gagaggaaga tgagcagcga cgccgcaagg aggaggaaga
     1261 gagaaggatg aaattggaga tggaggcaaa acgcaaacaa gaagaggaag aacggaagaa
     1321 acgggaggat gatgaaaagc ggaagaaaaa gggatctgga gggtcagggg gcgcgcccat
     1381 ggtttcaaag ggagaagagt tgttcacagg ggttgtaccg attcttgtag aacttgacgg
     1441 agatgtcaac gggcacaagt ttagtgtaag tggagaaggc gagggcgatg cgacctatgg
     1501 aaaactgacc cttaaattta tttgtactac gggtaaattg cccgttccct ggccgacatt
     1561 ggtaactaca ttcggatatg gtttgatgtg ctttgcaagg tatccggatc acatgaagca
     1621 gcatgatttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1681 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1741 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1801 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1861 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1921 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1981 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2041 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgc
     2101 gcccggagga atggtttcaa agggagaaga gttgttcaca ggggttgtac cgattcttgt
     2161 agaacttgac ggagatgtca acgggcacaa gtttagtgta agtggagaag gcgagggcga
     2221 tgcgacctat ggaaaactga cccttaaatt tatttgtact acgggtaaat tgcccgttcc
     2281 ctggccgaca ttggtaacta cattcggata tggtttgatg tgctttgcaa ggtatccgga
     2341 tcacatgaag cagcatgatt tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     2401 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     2461 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     2521 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     2581 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     2641 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     2701 cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga
     2761 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     2821 gtacaaggga tccggaggaa tggtttcaaa gggagaagag ttgttcacag gggttgtacc
     2881 gattcttgta gaacttgacg gagatgtcaa cgggcacaag tttagtgtaa gtggagaagg
     2941 cgagggcgat gcgacctatg gaaaactgac ccttaaattt atttgtacta cgggtaaatt
     3001 gcccgttccc tggccgacat tggtaactac attcggatat ggtttgatgt gctttgcaag
     3061 gtatccggat cacatgaagc agcatgattt cttcaagtcc gccatgcccg aaggctacgt
     3121 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     3181 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     3241 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     3301 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     3361 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     3421 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     3481 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     3541 ggacgagctg tacaagggat ccggtggcac cggtggatca ggctcttctc gaggttacta
     3601 ccatggtatt tgggtgggtg aagaaggtcg actttctcga ggtggagaac aaaaacttat
     3661 ttctgaagaa gatctgtgat ctagaccggt cgatatcaag cttatcgata atcaacctct
     3721 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     3781 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     3841 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     3901 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     3961 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     4021 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4081 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     4141 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4201 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     4261 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga
     4321 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     4381 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     4441 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     4501 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     4561 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     4621 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
     4681 caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     4741 ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg
     4801 agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     4861 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     4921 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     4981 gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     5041 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     5101 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     5161 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     5221 acaatagcag gcatgctggg gatgcggcgg gctctatggc ttctgaggcg gaaagaacca
     5281 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5341 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5401 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5461 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5521 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5581 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5641 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5701 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     5761 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5821 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5881 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5941 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6001 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6061 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     6121 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     6181 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     6241 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     6301 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     6361 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     6421 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     6481 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     6541 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     6601 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     6661 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     6721 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6781 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     6841 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     6901 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     6961 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7021 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7081 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7141 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7201 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7261 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7321 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7381 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7441 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7501 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7561 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7621 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7681 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7741 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7801 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7861 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7921 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7981 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8041 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8101 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8161 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8221 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8281 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8341 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8401 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8461 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8521 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8581 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8641 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8701 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8761 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8821 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8881 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8941 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9001 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
     9061 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     9121 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     9181 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     9241 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
     9301 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     9361 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     9421 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     9481 acttggcagt acatcaagtg tatcatatgc caagcacgcc ccctattgac gtcaatgacg
     9541 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     9601 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     9661 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     9721 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     9781 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt
     9841 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     9901 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     9961 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    10021 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
    10081 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
    10141 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
    10201 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
    10261 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
    10321 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
    10381 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
    10441 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
    10501 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
    10561 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
    10621 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
    10681 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
    10741 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    10801 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    10861 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
    10921 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    10981 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    11041 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    11101 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    11161 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
    11221 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
    11281 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
    11341 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
    11401 aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt
    11461 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
    11521 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
    11581 ttattacagg gacagcagag atccagtttg gttaattaag tgtctagact gcagagggcc
    11641 ctgcgtatg
//
