LOCUS       sequence-311276-       10700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BRET biosensor for detection of free G-beta-gamma in lentiviral 
            vector pLenti-hSyn.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10700)
  AUTHORS   Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, 
            Garcia-Marcos M
  TITLE     Revealing the Activity of Trimeric G-proteins in Live Cells with a 
            Versatile Biosensor Design.
  JOURNAL   Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020.
  PUBMED    32634377
REFERENCE   2  (bases 1 to 10700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020."
COMMENT     Sequence Label: pLenti-hSyn-G-beta-gamma-BERKY1
FEATURES             Location/Qualifiers
     source          1..10700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..448
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      485..494
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             566..1078
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             1103..1123
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(1685..1704)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1844..2095
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     2032..2053
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2698..2727
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    2761..3349
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2814..2834)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3232..3243)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3352..3368)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3353..3369)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3874..4054
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4080..4097)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4086..4310
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4356..4784
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4443..4462)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4653..4674
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4793..4813)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4798..5127
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4978..5113
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5040..5059
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5175..5222
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5241..5615
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5745..5866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5782..5801)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5836..5855
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5915..5931)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5915..5931)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5928..5950)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5939..5955
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5963..5969,5970..5987,5988..5993))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6008..6029
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6146..6163)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6317..6905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6397..6416)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7076..7867,7868..7936))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7699..7718
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7937..8041)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8116..8135)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8307..8686
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8688..8886
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8841..8861
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8904..9084
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9131..9256
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9749..9982
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10167..10211
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10509..10626
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
       61 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      121 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      181 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      241 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      301 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      361 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      421 aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg ctagaggatc cttcgaaagc
      481 tagcgccacc atgggatgta taaaatcaaa agggaaagac agcaagcttg gatcaggcgg
      541 tagcggggga tcaggcggta gcgggatggt cttcacactc gaagatttcg ttggggactg
      601 gcgacagaca gccggctaca acctggacca agtccttgaa cagggaggtg tgtccagttt
      661 gtttcagaat ctcggggtgt ccgtaactcc gatccaaagg attgtcctga gcggtgaaaa
      721 tgggctgaag atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg gcgaccaaat
      781 gggccagatc gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc actttaaggt
      841 gatcctgcac tatggcacac tggtaatcga cggggttacg ccgaacatga tcgactattt
      901 cggacggccg tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg taacagggac
      961 cctgtggaac ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg gctccctgct
     1021 gttccgagta accatcaacg gagtgaccgg ctggcggctg tgcgaacgca ttctggcgga
     1081 attcggatct ggtggttcag gcgaaaacct gtattttcag ggcggaagcg gagaagagga
     1141 ggaaaagaaa aaacaacagg aggaggaggc ggagaggctg aggaggatac aagaggaaat
     1201 ggaaaaagaa agaaagcggc gagaggaaga tgagcagcga cgccgcaagg aggaggaaga
     1261 gagaaggatg aaattggaga tggaggcaaa acgcaaacaa gaagaggaag aacggaagaa
     1321 acgggaggat gatgaaaagc ggaagaaaaa gggatctgga gggtcagggg gcgcgcccat
     1381 ggtttcaaag ggagaagagt tgttcacagg ggttgtaccg attcttgtag aacttgacgg
     1441 agatgtcaac gggcacaagt ttagtgtaag tggagaaggc gagggcgatg cgacctatgg
     1501 aaaactgacc cttaaattta tttgtactac gggtaaattg cccgttccct ggccgacatt
     1561 ggtaactaca ttcggatatg gtttgatgtg ctttgcaagg tatccggatc acatgaagca
     1621 gcatgatttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1681 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1741 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1801 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1861 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1921 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1981 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2041 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggatc
     2101 cggtggcacc ggtggatcag gctcttctcg agagaacttc cctctggtga tctctgagcg
     2161 ctggcagcag gaagtggcgg aaacagttta tgaagcagta aatgcagaca cggataaaat
     2221 cgaggccagg aagagagcta aaaataagca gcttggccac gaagaagatt acgccctggg
     2281 aagagactgc atcgtgcacg ggtacatgct gaagctgggg aaccctttcc tgacccagtg
     2341 gcagcgccgc tatttttacc tctttccgaa cagacttgag tggagaggag aaggcgagtc
     2401 gcgacaaagt ttactgacaa tggaacagat tgtgtccgtg gaagaaactc agattaaaga
     2461 caaaaagtgc attttgttga gaataaaagg agggaagcag ttcgttttgc agtgtgagag
     2521 tgacccagag tttgtgcagt ggaagaaaga gctgacggag acattcatgg aggcccagcg
     2581 gctgctacgg cgagccccca agttcctcaa caaatcccgc tcagccgtcg tggaactctc
     2641 aaagcctccc ctctgccata ggaacagcaa cggcctcgga ggttgactcg aggtggagaa
     2701 caaaaactta tttctgaaga agatctgtga tctagaccgg tcgatatcaa gcttatcgat
     2761 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     2821 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     2881 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     2941 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     3001 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     3061 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     3121 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     3181 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     3241 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     3301 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     3361 cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     3421 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     3481 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     3541 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     3601 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
     3661 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
     3721 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
     3781 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
     3841 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
     3901 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     3961 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     4021 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc
     4081 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     4141 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4201 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     4261 ggattgggaa gacaatagca ggcatgctgg ggatgcggcg ggctctatgg cttctgaggc
     4321 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     4381 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4441 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4501 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4561 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4621 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4681 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4741 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     4801 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4861 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4921 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4981 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     5041 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     5101 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     5161 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     5221 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     5281 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     5341 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     5401 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     5461 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     5521 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     5581 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
     5641 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     5701 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     5761 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5821 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     5881 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     5941 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6001 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6061 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6121 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6181 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6241 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6301 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6361 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6421 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6481 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6541 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6601 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6661 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6721 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6781 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6841 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6901 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6961 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7021 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7081 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7141 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7201 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7261 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7321 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7381 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7441 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7501 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7561 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7621 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7681 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7741 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7801 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7861 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7921 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7981 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8041 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     8101 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     8161 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     8221 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     8281 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
     8341 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     8401 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     8461 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     8521 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagcacgc cccctattga
     8581 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     8641 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     8701 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     8761 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     8821 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     8881 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8941 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     9001 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     9061 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     9121 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     9181 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     9241 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     9301 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     9361 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     9421 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     9481 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     9541 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     9601 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     9661 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     9721 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     9781 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     9841 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     9901 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     9961 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
    10021 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
    10081 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
    10141 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
    10201 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
    10261 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
    10321 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
    10381 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
    10441 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
    10501 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
    10561 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
    10621 aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa gtgtctagac
    10681 tgcagagggc cctgcgtatg
//
