LOCUS       sequence-311277-       10553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BRET biosensor for detection of G-alpha-q-GTP in lentiviral vector 
            pLenti-hSyn.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10553)
  AUTHORS   Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, 
            Garcia-Marcos M
  TITLE     Revealing the Activity of Trimeric G-proteins in Live Cells with a 
            Versatile Biosensor Design.
  JOURNAL   Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020.
  PUBMED    32634377
REFERENCE   2  (bases 1 to 10553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020."
COMMENT     Sequence Label: pLenti-hSyn-G-alpha-q*-BERKY1
FEATURES             Location/Qualifiers
     source          1..10553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..448
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      485..494
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             566..1078
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             1103..1123
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(1685..1704)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1844..2095
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     2032..2053
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2551..2580
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    2614..3202
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2667..2687)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3085..3096)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3205..3221)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3206..3222)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3727..3907
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3933..3950)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3939..4163
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4209..4637
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4296..4315)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4506..4527
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4646..4666)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4651..4980
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4831..4966
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4893..4912
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5028..5075
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5094..5468
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5598..5719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5635..5654)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5689..5708
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5768..5784)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5768..5784)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5781..5803)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5792..5808
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5816..5822,5823..5840,5841..5846))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5861..5882
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5999..6016)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6170..6758)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6250..6269)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6929..7720,7721..7789))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7552..7571
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7790..7894)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7969..7988)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8160..8539
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8541..8739
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8694..8714
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8757..8937
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8984..9109
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9602..9835
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10020..10064
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10362..10479
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
       61 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      121 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      181 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      241 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      301 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      361 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      421 aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg ctagaggatc cttcgaaagc
      481 tagcgccacc atgggatgta taaaatcaaa agggaaagac agcaagcttg gatcaggcgg
      541 tagcggggga tcaggcggta gcgggatggt cttcacactc gaagatttcg ttggggactg
      601 gcgacagaca gccggctaca acctggacca agtccttgaa cagggaggtg tgtccagttt
      661 gtttcagaat ctcggggtgt ccgtaactcc gatccaaagg attgtcctga gcggtgaaaa
      721 tgggctgaag atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg gcgaccaaat
      781 gggccagatc gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc actttaaggt
      841 gatcctgcac tatggcacac tggtaatcga cggggttacg ccgaacatga tcgactattt
      901 cggacggccg tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg taacagggac
      961 cctgtggaac ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg gctccctgct
     1021 gttccgagta accatcaacg gagtgaccgg ctggcggctg tgcgaacgca ttctggcgga
     1081 attcggatct ggtggttcag gcgaaaacct gtattttcag ggcggaagcg gagaagagga
     1141 ggaaaagaaa aaacaacagg aggaggaggc ggagaggctg aggaggatac aagaggaaat
     1201 ggaaaaagaa agaaagcggc gagaggaaga tgagcagcga cgccgcaagg aggaggaaga
     1261 gagaaggatg aaattggaga tggaggcaaa acgcaaacaa gaagaggaag aacggaagaa
     1321 acgggaggat gatgaaaagc ggaagaaaaa gggatctgga gggtcagggg gcgcgcccat
     1381 ggtttcaaag ggagaagagt tgttcacagg ggttgtaccg attcttgtag aacttgacgg
     1441 agatgtcaac gggcacaagt ttagtgtaag tggagaaggc gagggcgatg cgacctatgg
     1501 aaaactgacc cttaaattta tttgtactac gggtaaattg cccgttccct ggccgacatt
     1561 ggtaactaca ttcggatatg gtttgatgtg ctttgcaagg tatccggatc acatgaagca
     1621 gcatgatttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1681 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1741 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1801 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1861 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1921 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1981 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2041 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggatc
     2101 cggtggcacc ggtggatcag gctcttctcg agagaagtac ctggaggacc ggggcgaggt
     2161 gacttttgag aagatcttct cccagaagct ggggtacctg cttttccgag acttctgcct
     2221 gaagcacctg gaggaggcca agcccttggt agagttctac gaggagatca agaaatacga
     2281 gaagctggag acagaggagg agcgcctggt ctgcagccga gagatcttcg acacgtacat
     2341 catgaaggag ctgctggcct gctcacatcc tttctcgaag agcgccattg agcacgtcca
     2401 gggccatctg gtgaagaagc aggtgcctcc ggatctcttc cagccatata ttgaagaaat
     2461 ttgccagaac ctccgaggag acgtgttcca gaaattcatc gagagcgata aattcacacg
     2521 gttttgccag tggaagtgac tcgaggtgga gaacaaaaac ttatttctga agaagatctg
     2581 tgatctagac cggtcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     2641 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2701 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2761 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2821 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2881 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2941 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3001 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     3061 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     3121 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     3181 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga
     3241 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     3301 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     3361 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     3421 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     3481 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     3541 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     3601 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     3661 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     3721 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     3781 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     3841 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     3901 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     3961 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     4021 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     4081 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     4141 tggggatgcg gcgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     4201 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4261 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4321 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4381 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4441 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4501 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4561 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4621 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4681 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     4741 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4801 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4861 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     4921 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     4981 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
     5041 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
     5101 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
     5161 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
     5221 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
     5281 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
     5341 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
     5401 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
     5461 aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     5521 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     5581 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5641 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5701 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     5761 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5821 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5881 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     5941 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6001 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6061 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6121 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6181 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6241 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6301 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6361 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6421 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6481 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6541 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6601 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6661 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6721 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6781 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6841 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6901 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6961 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7021 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7081 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7141 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7201 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7261 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7321 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7381 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7441 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7501 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7561 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7621 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7681 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7741 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7801 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7861 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7921 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
     7981 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     8041 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     8101 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
     8161 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     8221 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     8281 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     8341 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     8401 agtgtatcat atgccaagca cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     8461 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     8521 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     8581 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     8641 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     8701 gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt
     8761 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8821 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8881 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     8941 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     9001 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     9061 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     9121 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     9181 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     9241 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     9301 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     9361 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     9421 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     9481 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     9541 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     9601 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     9661 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     9721 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     9781 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     9841 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     9901 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     9961 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
    10021 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
    10081 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
    10141 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
    10201 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
    10261 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc
    10321 gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
    10381 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
    10441 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
    10501 agagatccag tttggttaat taagtgtcta gactgcagag ggccctgcgt atg
//
