LOCUS       sequence-31128-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Kim JS, Lee C, Bonifant CL, Ressom H, Waldman T
  TITLE     Activation of p53-dependent growth suppression in human cells by 
            mutations in PTEN or PIK3CA.
  JOURNAL   Mol Cell Biol. 2007 Jan . 27(2):662-77.
  PUBMED    17060456
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2007 Jan . 27(2):662-77."
COMMENT     Sequence Label: pLKO-p53-shRNA-427
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    130..247
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        296..779
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     725..743
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 ggctttatat atcttgtgga aaggacgaaa caccgggtcc agatgaagct cccagaactc
       61 gagttctggg agcttcatct ggacttttta attctcgacc tcgagacaaa tggcagtatt
      121 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
      181 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
      241 aaattttcgg gtttattaca gggacagcag agatccactt tggccgcggc tcgagggggt
      301 tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
      361 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
      421 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
      481 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
      541 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
      601 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
      661 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg
      721 cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg
      781 accgaatcac cgacctctct
//
