LOCUS       sequence-311287-        8506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid constitutively expressing the Cas9 nuclease and the 
            non-coding tracrRNA from S. pyogenes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8506)
  AUTHORS   Velazquez E, Lorenzo V, Al-Ramahi Y
  TITLE     Recombination-Independent Genome Editing through 
            CRISPR/Cas9-Enhanced TargeTron Delivery.
  JOURNAL   ACS Synth Biol. 2019 Sep 20;8(9):2186-2193. doi: 
            10.1021/acssynbio.9b00293. Epub 2019 Sep 3.
  PUBMED    31419111
REFERENCE   2  (bases 1 to 8506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00293"; journalName: "ACS Synth Biol"; date: 
            "2019-09-20- 20"; volume: "8"; issue: "9"; pages: "2186-2193"
COMMENT     Sequence Label: pSEVA421-Cas9tr
FEATURES             Location/Qualifiers
     source          1..8506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..31
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      155..249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(261..4367)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(264..4367)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_RNA        4663..4741
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     CDS             5109..5900
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            6046..6154
                     /note="incP origin of transfer"
     CDS             complement(6215..7363)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      7756..8287
                     /label=oriV
                     /note="incP origin of replication"
     terminator      complement(8414..8500)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc tcagtcacct cctagctgac tcaaatcaat gcgtgtttca
      301 taaagaccag tgatggattg atggataaga gtggcatcta aaacttcttt tgtagacgta
      361 tatcgtttac gatcaattgt tgtatcaaaa tatttaaaag cagcgggagc tccaagattc
      421 gtcaacgtaa ataaatgaat aatattttct gcttgttcac gtattggttt gtctctatgt
      481 ttgttatatg cactaagaac tttatctaaa ttggcatctg ctaaaataac acgcttagaa
      541 aattcactga tttgctcaat aatctcatct aaataatgct tatgctgctc cacaaacaat
      601 tgtttttgtt cgttatcttc tggactaccc ttcaactttt cataatgact agctaaatat
      661 aaaaaattca catatttgct tggcagagcc agctcatttc ctttttgtaa ttctccggca
      721 ctagccagca tccgtttacg accgttttct aactcaaaaa gactatattt aggtagttta
      781 atgattaagt cttttttaac ttccttatat cctttagctt ctaaaaagtc aatcggattt
      841 ttttcaaagg aacttctttc cataattgtg atccctagta actctttaac ggattttaac
      901 ttcttcgatt tccctttttc caccttagca accactagga ctgaataagc taccgttgga
      961 ctatcaaaac caccatattt ttttggatcc cagtcttttt tacgagcaat aagcttgtcc
     1021 gaatttcttt ttggtaaaat tgactccttg gagaatccgc ctgtctgtac ttctgttttc
     1081 ttgacaatat tgacttgggg catggacaat actttgcgca ctgtggcaaa atctcgccct
     1141 ttatcccaga caatttctcc agtttcccca ttagtttcga ttagagggcg tttgcgaatc
     1201 tctccatttg caagtgtaat ttctgttttg aagaagttca tgatattaga gtaaaagaaa
     1261 tattttgcgg ttgctttgcc tatttcttgc tcagacttag caatcatttt acgaacatca
     1321 taaactttat aatcaccata gacaaactcc gattcaagtt ttggatattt cttaatcaaa
     1381 gcagttccaa cgacggcatt tagatacgca tcatgggcat gatggtaatt gttaatctca
     1441 cgtactttat agaattggaa atcttttcgg aagtcagaaa ctaatttaga ttttaaggta
     1501 atcactttaa cctctcgaat aagtttatca ttttcatcgt atttagtatt catgcgacta
     1561 tccaaaattt gtgccacatg cttagtgatt tggcgagttt caaccaattg gcgtttgata
     1621 aaaccagctt tatcaagttc actcaaacct ccacgttcag ctttcgttaa attatcaaac
     1681 ttacgttgag tgattaactt ggcgtttaga agttgtctcc aatagttttt catctttttg
     1741 actacttctt cacttggaac gttatccgat ttaccacgat ttttatcaga acgcgttaag
     1801 accttattgt ctattgaatc gtctttaagg aaactttgtg gaacaatgtg atcgacatca
     1861 taatcactta aacgattaat atctaattct tggtccacat acatgtctct tccattttgg
     1921 agataataga gatagagctt ttcattttgc aattgagtat tttcaacagg atgctcttta
     1981 agaatctgac ttcctaattc tttgatacct tcttcgattc gtttcatacg ctctcgcgaa
     2041 tttttctggc ccttttgagt tgtctgattt tcacgtgcca tttcaataac gatattttct
     2101 ggcttatgcc gccccattac tttgaccaat tcatcaacaa cttttacagt ctgtaaaata
     2161 ccttttttaa tagcagggct accagctaaa tttgcaatat gttcatgtaa actatcgcct
     2221 tgtccagaca cttgtgcttt ttgaatgtct tctttaaatg tcaaactatc atcatggatc
     2281 agctgcataa aattgcgatt ggcaaaacca tctgatttca aaaaatctaa tattgttttg
     2341 ccagattgct tatccctaat accattaatc aattttcgag acaaacgtcc ccaaccagta
     2401 taacggcgac gtttaagctg tttcatcacc ttatcatcaa agaggtgagc atatgtttta
     2461 agtctttcct caatcatctc cctatcttca aataaggtca atgttaaaac aatatcctct
     2521 aagatatctt cattttcttc attatccaaa aaatctttat ctttaataat ttttagcaaa
     2581 tcatggtagg tacctaatga agcattaaat ctatcttcaa ctcctgaaat ttcaacacta
     2641 tcaaaacatt ctattttttt gaaataatct tcttttaatt gcttaacggt tacttttcga
     2701 tttgttttga agagtaaatc aacaatggct ttcttctgtt cacctgaaag aaatgctggt
     2761 tttcgcattc cttcagtaac atatttgacc tttgtcaatt cgttataaac cgtaaaatac
     2821 tcataaagca aactatgttt tggtagtact ttttcatttg gaagattttt atcaaagttt
     2881 gtcatgcgtt caataaatga ttgagctgaa gcacctttat cgacaacttc ttcaaaattc
     2941 catggggtaa ttgtttcttc agacttccga gtcatccatg caaaacgact attgccacgc
     3001 gccaatggac caacataata aggaattcga aaagtcaaga ttttttcaat cttctcacga
     3061 ttgtctttta aaaatggata aaagtcttct tgtcttctca aaatagcatg cagctcaccc
     3121 aagtgaattt gatggggaat agagccgttg tcaaaggtcc gttgcttgcg cagcaaatct
     3181 tcacgattta gtttcaccaa taattcctca gtaccatcca ttttttctaa aattggtttg
     3241 ataaatttat aaaattcttc ttggctagct cccccatcaa tataacctgc atatccgttt
     3301 tttgattgat caaaaaagat ttctttatac ttttctggaa gttgttgtcg aactaaagct
     3361 tttaaaagag tcaagtcttg atgatgttca tcgtagcgtt taatcattga agctgatagg
     3421 ggagccttag ttatttcagt atttactctt aggatatctg aaagtaaaat agcatctgat
     3481 aaattcttag ctgccaaaaa caaatcagca tattgatctc caatttgcgc caataaatta
     3541 tctaaatcat catcgtaagt atcttttgaa agctgtaatt tagcatcttc tgccaaatca
     3601 aaatttgatt taaaattagg ggtcaaaccc aatgacaaag caatgagatt cccaaataag
     3661 ccatttttct tctcaccggg gagctgagca atgagatttt ctaatcgtct tgatttactc
     3721 aatcgtgcag aaagaatcgc tttagcatct actccacttg cgttaatagg gttttcttca
     3781 aataattgat tgtaggtttg taccaactgg ataaatagtt tgtccacatc actattatca
     3841 ggatttaaat ctccctcaat caaaaaatga ccacgaaact taatcatatg cgctaaggcc
     3901 aaatagatta agcgcaaatc cgctttatca gtagaatcta ccaatttttt tcgcagatga
     3961 tagatagttg gatatttctc atgataagca acttcatcta ctatatttcc aaaaatagga
     4021 tgacgttcat gcttcttgtc ttcttccacc aaaaaagact cttcaagtcg atgaaagaaa
     4081 ctatcatcta ctttcgccat ctcatttgaa aaaatctcct gtagataaca aatacgattc
     4141 ttccgacgtg tataccttct acgagctgtc cgtttgagac gagtcgcttc cgctgtctct
     4201 ccactgtcaa ataaaagagc ccctataaga ttttttttga tactgtggcg gtctgtattt
     4261 cccagaacct tgaacttttt agacggaacc ttatattcat cagtgatcac cgcccatccg
     4321 acgctatttg tgccgatatc taagcctatt gagtatttct tatccatttt tgcctcctaa
     4381 aataaaaagt ttaaattaaa tccataatga gtttgatgat ttcaataata gttttaatga
     4441 cctccgaaat tagtttaata tgctttaatt tttctttttc aaaatatctc ttcaaaaaat
     4501 attacccaat acttaataat aaatagatta taacacaaaa ttcttttaaa aagtagttta
     4561 ttttgttatc attctatagt attaagtatt gttttatggc tgataaattt ctttgaattt
     4621 ctccttgatt atttgttata aaagttataa aataatcttg ttggaaccat tcaaaacagc
     4681 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
     4741 ctttttttga tacttctatt ctactctgac tgcaaaccaa aaaaacaagc gctttcaaaa
     4801 cgcttgtttt atcattttta gggaaattaa tctcttaatc cttttatcat tctacattta
     4861 ggcgctgcca tcttgctaaa cctactaagc tccacaggat gatatttaaa tgaaccttga
     4921 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     4981 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     5041 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     5101 taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     5161 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     5221 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     5281 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     5341 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     5401 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     5461 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     5521 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     5581 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     5641 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     5701 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     5761 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     5821 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     5881 ccaaggtagt cggcaaataa gacaattgtc cttttccgct gcataaccct gcttcggggt
     5941 cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca
     6001 aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga
     6061 agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt
     6121 gctcaacggg aatcctgctc tgcgaggctg gccgtaggcc ggccgcgatg caggtggctg
     6181 ctgaaccccc agccggaact gaccccacaa ggccctagcg tttgcaatgc accaggtcat
     6241 cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga
     6301 cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc gcacatgagg cggaaggttt
     6361 ccagcttgag cgggtacggc tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg
     6421 tcggcgacag cttgcggtac ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca
     6481 gcacgacgat ttcctcgtcg atcaggacct ggcaacggga cgttttcttg ccacggtcca
     6541 ggacgcggaa gcggtgcagc agcgacaccg attccaggtg cccaacgcgg tcggacgtga
     6601 agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg taataccggc
     6661 cattgatcga ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc
     6721 cgataggggt gcgcttcgcg tactccaaca cctgctgcca caccagttcg tcatcgtcgg
     6781 cccgcagctc gacgccggtg taggtgatct tcacgtcctt gttgacgtgg aaaatgacct
     6841 tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg gcagagcggg
     6901 ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa
     6961 gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc ttggcttcgc
     7021 tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc atagttcctc
     7081 gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc ctgttcgaga cgacgcgaac
     7141 gctccacggc ggccgatggc gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct
     7201 cgatcttggc cgtagcttgc tggactatcg agccgacgga ctggaaggtt tcgcggggcg
     7261 cacgcatgac ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga
     7321 tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt cattcaccct ccttgcggga
     7381 ttgccccgga attaattccc cggatcgatc cgtcgatctt gatcccctgc gccatcagat
     7441 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
     7501 cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt ctagctatcg
     7561 ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt cccttgtcca
     7621 gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta
     7681 cgtggctgcc atttttgggg tgaggccgtt cgcggccgag gggcgcagcc cctgggggga
     7741 tgggaggccc gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc cccttcggcg
     7801 tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac aaggtttata aatattggtt
     7861 taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa
     7921 tgctggattt tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc cctcatctgt
     7981 cagcactctg cccctcaagt gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc
     8041 tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac
     8101 tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg ccagctccac gtcgccggcc
     8161 gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt
     8221 caacgtccgc ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc
     8281 ggcggcccta catggctctg ctgtagtgag tgggttgcgc tccggcagcg gtcctgatcc
     8341 cccgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacggcg cgcccagctg
     8401 tctagggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     8461 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcct
//
