LOCUS       sequence-31129-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Mikolajczyk J, Drag M, Bekes M, Cao JT, Ronai Z, Salvesen GS
  TITLE     Small ubiquitin-related modifier (SUMO)-specific proteases: 
            profiling the specificities and activities of human SENPs.
  JOURNAL   J Biol Chem. 2007 Sep 7. 282(36):26217-24.
  PUBMED    17591783
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Sep 7. 282(36):26217-24."
COMMENT     Sequence Label: pET28a-pro-sumo1
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             363..385
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    400..424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(424..443)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(425..443)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(527..546)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(848..867)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagaactgtt
       61 gaatgacctc ccgtttgttc ctgataaact tcaatcacat cttcttcctc cattcccagt
      121 tcttttggag tatgattatc agcaattctc tgaccctcaa agagaaacct gagtgaattc
      181 attggaacac cctgtctttg acagtatgat tctttgagtt tcttgagatg tgttgtcatt
      241 ttcactttga agtgaatctc actgctatcc tgtccaatga ctttgagttt aatatattca
      301 ccttccttct tatcccccaa gtcctcagtt gaaggttttg cctcctggtc agccatggta
      361 tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa
      421 ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctacgccgga
      481 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
      541 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
      601 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
      661 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
      721 gagtcgcata anggagagcg tcgagatccc ggacaccatc gaatggcgca aaacctttcg
      781 cggtatggca tgatagcgcc cggaagagag tcaattcagg tggtgaatgt gaaaccagta
      841 acgttatacg atgtcgcaga gtatgccggt gtctcttatc
//
