LOCUS       sequence-31130-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Mikolajczyk J, Drag M, Bekes M, Cao JT, Ronai Z, Salvesen GS
  TITLE     Small ubiquitin-related modifier (SUMO)-specific proteases: 
            profiling the specificities and activities of human SENPs.
  JOURNAL   J Biol Chem. 2007 Sep 7. 282(36):26217-24.
  PUBMED    17591783
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Sep 7. 282(36):26217-24."
COMMENT     Sequence Label: pET28a-pro-sumo2
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(51..329)
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 2"
                     /label=SUMO2
                     /translation="MADEKPKEGVKTENNDHINLKVAGQDGSVVQFKIKRHTPLSKLMK
                     AYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG"
     RBS             336..358
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    373..397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(397..416)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(398..416)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(500..519)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(821..840)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgaggtagac acctcccgtc
       61 tgctgttgga acacatcaat tgtatcttca tcctccattt ccaactgtgc aggtgtgtct
      121 gtttcattga ttggttgccc gtcaaatcgg aatctgatct gcctcattga caatccctgt
      181 cgttcacaat aggctttcat tagtttacta agtggtgtat gcctcttaat cttaaactgc
      241 accacagaac catcctgccc cgccaccttc aaattaatat gatcgttgtt ctcagtcttg
      301 actccttcct tgggcttttc gtcggccatg gtatatctcc ttcttaaagt taaacaaaat
      361 tatttctaga ggggaattgt tatccgctca caattcccct atagtgagtc gtattaattt
      421 cgcgggatcg agatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
      481 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
      541 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
      601 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
      661 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagat
      721 cccggacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
      781 gagtcaattc nggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc
      841 ggtgtctctt atcagaccgt ttcccgcgtg tgaaccaggc cagccacgtt tc
//
