LOCUS       sequence-311305-       14342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS 
            mutant construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14342)
  AUTHORS   Lopacinski AB, Sweatt AJ, Smolko CM, Gray-Gaillard E, Borgman CA, 
            Shah M, Janes KA
  TITLE     Modeling the complete kinetics of coxsackievirus B3 reveals human 
            determinants of host-cell feedback.
  JOURNAL   Cell Syst. 2021 Apr 21;12(4):304-323.e13. doi: 
            10.1016/j.cels.2021.02.004. Epub 2021 Mar 18.
  PUBMED    33740397
REFERENCE   2  (bases 1 to 14342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2021.02.004"; journalName: "Cell Syst"; date: 
            "2021-04-21- 21"; volume: "12"; issue: "4"; pages: "304-323.e13"
COMMENT     Sequence Label: pSLIK 3xFLAG-MAVS-Gln93 hygro
FEATURES             Location/Qualifiers
     source          1..14342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(97..116)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        288..667
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        669..867
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             885..1065
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1112..1237
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1730..1963
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2148..2192
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2490..2607
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2664..2688
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2720..3034
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2728..2746
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2764..2782
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2799..2817
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2835..2853
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2871..2889
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2906..2924
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2942..2960
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3007..3031
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3043..3108
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(4773..4797)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4847..6058
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5895..5914
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6183..6800,6801..6806,6807..6842,6843..6845,6846..6881,
                     6882..6884,6885..6920,6921..6929)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7074..7536
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7130..7147)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7357..7376
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7595..8614
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             7595..8614
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    9080..9668
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9133..9153)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9551..9562)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9671..9687)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9672..9688)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10197..10377
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10403..10420)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10409..10633
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10679..11107
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10766..10785)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10976..10997
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11116..11136)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11121..11450
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11301..11436
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11363..11382
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11498..11545
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11564..11938
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12068..12189
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12105..12124)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12159..12178
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12238..12254)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12238..12254)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12251..12273)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12262..12278
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12286..12292,12293..12310,12311..12316))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12331..12352
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12469..12486)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12640..13228)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12720..12739)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13399..14190,14191..14259))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14022..14041
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(14260..14342,1..22))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
       61 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
      121 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
      181 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
      241 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
      301 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      361 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      421 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      481 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      541 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      601 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      661 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      721 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      781 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      841 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
      901 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      961 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     1021 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     1081 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     1141 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     1201 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     1261 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     1321 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     1381 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     1441 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1501 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1561 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1621 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1681 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1741 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1801 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1861 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1921 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1981 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     2041 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     2101 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     2161 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     2221 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     2281 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     2341 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     2401 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
     2461 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     2521 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2581 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     2641 tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt aaaggaacca
     2701 attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
     2761 tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg atagagaacg
     2821 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
     2881 tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat gtcgagttta
     2941 ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg aggcctatat
     3001 aagcagagct cgtttagtga accgtcagat cgccactaga tggactacaa agaccatgac
     3061 ggtgattata aagatcatga catcgattac aaggatgacg atgacaagac tagtatgccg
     3121 tttgctgaag acaagaccta taagtatatc tgccgcaatt tcagcaattt ttgcaatgtg
     3181 gatgttgtag agattctgcc ttacctgccc tgcctcacag caagagacca ggatcgactg
     3241 cgggccacct gcacactctc agggaaccgg gacaccctct ggcatctctt caataccctt
     3301 cagcggcggc ccggctgggt ggagtacttc attgcggcac tgaggggctg tgagctagtt
     3361 gatctcgcgg acgaagtggc ctctgtctac cagagctacc agcctcggac ctcggaccgt
     3421 cccccagacc cactggagcc accgtcactt cctgctgaga ggccagggcc ccccacacct
     3481 gctgcggccc acagcatccc ctacaacagc tgcagagaga aggagccaag ttaccccatg
     3541 cctgtccagg agacccaggc gccagagtcc ccaggagaga attcagagca agccctgcag
     3601 acgctcagcc ccagagccat cccaaggaat ccagatggtg gccccctgga gtcctcctct
     3661 gacctggcag ccctcagccc tctgacctcc agcgggcatc aggagaagga cacagaactg
     3721 ggcagtaccc acacagcagg tgcgacctcc agcctcacac catcccgtgg gcctgtgtct
     3781 ccatctgtct ccttccagcc cctggcccgt tccaccccca gggcaagccg cttgcctgga
     3841 cccacagggt cagttgtatc tactggcacc tccttctcct cctcatcccc tggcttggcc
     3901 tctgcagggg ctgcagaggg taaacagggt gcagagagtg accaggccga gcctatcatc
     3961 tgctccagtg gggcagaggc acctgccaac tctctgccct ccaaagtgcc taccaccttg
     4021 atgcctgtga acacagtggc cctgaaagtg cctgccaacc cagcatctgt cagcacagtg
     4081 ccctccaagt tgccaactag ctcaaagccc cctggtgcag tgccttctaa tgcgctcacc
     4141 aatccagcac catccaaatt gcccatcaac tcaacccgtg ctggcatggt gccatccaaa
     4201 gtgcctacta gcatggtgct caccaaggtg tctgccagca cagtccccac tgacgggagc
     4261 agcagaaatg aggagacccc agcagctcca acacccgccg gcgccactgg aggcagctca
     4321 gcctggctag acagcagctt tgagaatagg ggccttgggt cggagctgag taagcctggc
     4381 gtgctggcat cccaggtaga cagcccgttc tcgggctgct tcgaggatct tgccatcagt
     4441 gccagcacct ccttgggcat ggggccctgc catggcccag aggagaatga gtataagtcc
     4501 gagggcacct ttgggatcca cgtggctgag aaccccagca tccagctcct ggagggcaac
     4561 cctgggccac ctgcggaccc ggatggcggc cccaggccac aagccgaccg gaagttccag
     4621 gagagggagg tgccatgcca caggccctca cctggggctc tgtggctcca ggtggctgtg
     4681 acaggggtgc tggtagtcac actcctggtg gtgctgtacc ggcggcgtct gcactagcaa
     4741 ttggaagact aatgcgtttt cgagatatct agacccagct ttcttgtaca aagtggttga
     4801 taacatctcg agccataaag atggttaatt aacccaccca agatctggcc tccgcgccgg
     4861 gttttggcgc ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa
     4921 gggcgcagcg agcgtcctga tccttccgcc cggacgctca ggacagcggc ccgctgctca
     4981 taagactcgg ccttagaacc ccagtatcag cagaaggaca ttttaggacg ggacttgggt
     5041 gactctaggg cactggtttt ctttccagag agcggaacag gcgaggaaaa gtagtccctt
     5101 ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt atataaggac
     5161 gcgccgggtg tggcacagct agttccgtcg cagccgggat ttgggtcgcg gttcttgttt
     5221 gtggatcgct gtgatcgtca cttggtgagt agcgggctgc tgggctggcc ggggctttcg
     5281 tggccgccgg gccgctcggt gggacggaag cgtgtggaga gaccgccaag ggctgtagtc
     5341 tgggtccgcg agcaaggttg ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg
     5401 ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa
     5461 ggtggggggc atggtgggcg gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa
     5521 agctcttatt cgggtgagat gggctggggc accatctggg gaccctgacg tgaagtttgt
     5581 cactgactgg agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt
     5641 tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt
     5701 tctgttggct tataatgcag ggtggggcca cctgccggta ggtgtgcggt aggcttttct
     5761 ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc ctgaatcgac aggcgccgga
     5821 cctctggtga ggggagggat aagtgaggcg tcagtttctt tggtcggttt tatgtaccta
     5881 tcttcttaag tagctgaagc tccggttttg aactatgcgc tcggggttgg cgagtgtgtt
     5941 ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt gggtcaatat gtaattttca
     6001 gtgttagact agtaaattgt ccgctaaatt ctggccgttt ttggcttttt tgttagacga
     6061 agcttggtac cgagctcgga tctccacccc gtaccggtcc tgcaggattt aaatggcgcg
     6121 ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca gtcgaattca
     6181 ccatgtctag actggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg
     6241 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
     6301 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc
     6361 tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc
     6421 ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
     6481 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
     6541 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
     6601 ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga
     6661 cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc
     6721 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
     6781 agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc
     6841 tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg
     6901 attttgacct tgacatgctc cccgggtaac taagtaagga tccgcggccg cactagagga
     6961 attccgcccc tctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     7021 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     7081 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     7141 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     7201 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     7261 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     7321 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     7381 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     7441 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     7501 ggggacgtgg ttttcctttg aaaaacacga tgataagctt accggtacgc gtgatagctt
     7561 gccacaaccc gtaccaaaga tggatagatc cggaaagcct gaactcaccg cgacgtctgt
     7621 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
     7681 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
     7741 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
     7801 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
     7861 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
     7921 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
     7981 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
     8041 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     8101 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     8161 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
     8221 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
     8281 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
     8341 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
     8401 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
     8461 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
     8521 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
     8581 acgccccagc actcgtccga gggcaaagga atagagtaga tgccgaccga acaagagctg
     8641 atttcgagaa cgcctcagcc agcaactcgc gcgagcctag caaggcaaat gcgagagaac
     8701 ggccttacgc ttggtggcac agttctcgtc cacagttcgc taagctcgct cggctgggtc
     8761 gcgggagggc cggtcgcagt gattcaggcc cttctggatt gtgttggtcc ccagggcacg
     8821 attgtcatgc ccacgcactc gggtgatctg actgatcccg cagattggag atcgccgccc
     8881 gtgcctgccg attgggtgca gatctctaga tctgtacaag taaagcggcc gcgactctag
     8941 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     9001 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgtttagtc cctcccaatt
     9061 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     9121 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     9181 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     9241 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     9301 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     9361 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     9421 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     9481 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     9541 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     9601 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     9661 ctccccgcat cgataccgtc gacctcgatc gagacctaga aaaacatgga gcaatcacaa
     9721 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     9781 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     9841 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     9901 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     9961 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
    10021 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
    10081 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
    10141 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
    10201 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
    10261 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
    10321 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
    10381 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
    10441 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
    10501 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
    10561 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
    10621 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
    10681 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
    10741 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
    10801 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    10861 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
    10921 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    10981 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
    11041 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
    11101 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
    11161 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
    11221 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
    11281 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
    11341 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
    11401 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
    11461 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
    11521 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
    11581 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
    11641 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
    11701 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
    11761 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
    11821 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
    11881 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
    11941 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
    12001 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
    12061 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    12121 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    12181 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
    12241 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    12301 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    12361 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    12421 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    12481 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    12541 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    12601 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    12661 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    12721 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    12781 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    12841 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    12901 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    12961 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    13021 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    13081 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    13141 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    13201 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    13261 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    13321 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    13381 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    13441 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    13501 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    13561 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    13621 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    13681 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    13741 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    13801 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    13861 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    13921 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    13981 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    14041 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    14101 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    14161 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    14221 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    14281 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    14341 aa
//
