LOCUS       sequence-31131-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Mikolajczyk J, Drag M, Bekes M, Cao JT, Ronai Z, Salvesen GS
  TITLE     Small ubiquitin-related modifier (SUMO)-specific proteases: 
            profiling the specificities and activities of human SENPs.
  JOURNAL   J Biol Chem. 2007 Sep 7. 282(36):26217-24.
  PUBMED    17591783
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Sep 7. 282(36):26217-24."
COMMENT     Sequence Label: pET28a-pro-sumo3
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(86..361)
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 3"
                     /label=SUMO3
                     /translation="MAEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKA
                     YCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG"
     RBS             368..390
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    405..429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(429..448)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(430..448)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(532..551)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg aggaaactgt
       61 gccctgccag gctgctctcc ggcacacctc ccgtctgctg ctggaacacg tcgatggtgt
      121 cctcgtcctc catctccagc tgtgctggag tgtcagtttc attgattggc tgcccgtcga
      181 acctgaatct gatctgcctc attgacaagc cctgcctctc gcagtaggcc ttcatcagct
      241 tgctcagcgg cgtgtgcctc ttgattttga actgcaccac ggagccgtcc tgcccggcca
      301 ccttcaggtt gatgtggtca ttctctgtct tcacaccctc cttgggcttc tcctcggcca
      361 tggtatatct ccttcttaaa gttaaacaaa attatttcta gaggggaatt gttatccgct
      421 cacaattccc ctatagtgag tcgtattaat ttcgcgggat cgagatctcg atcctctacg
      481 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
      541 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
      601 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
      661 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
      721 tgcaggagtc gcataaggga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc
      781 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcnggtggtg aatgtgaaac
      841 cagtaacgtt atacgatgtc gcaga
//
