LOCUS       sequence-31132-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Mikolajczyk J, Drag M, Bekes M, Cao JT, Ronai Z, Salvesen GS
  TITLE     Small ubiquitin-related modifier (SUMO)-specific proteases: 
            profiling the specificities and activities of human SENPs.
  JOURNAL   J Biol Chem. 2007 Sep 7. 282(36):26217-24.
  PUBMED    17591783
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Sep 7. 282(36):26217-24."
COMMENT     Sequence Label: pcDNA3flag-RanGAPdeltaN
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             128..151
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(701..719)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(702..719)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(739..756)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    745..953
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agcagagctc tctggctanc tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctctagcc tcgacgttta gtgaaccgtc agaattgatc
      121 taccatggac tacaaagacg atgacgacaa gcttgatatc aacactgggg agccagctcc
      181 cgtgctgtcc tccccacctc ctgcagacgt ctccaccttc ctggcttttc cctctccaga
      241 gaagctgctg cgcctagggc ccaagagctc cgtgctgata gcccagcaga ctgacacgtc
      301 tgaccccgag aaggtggtct ctgccttcct aaaggtgtca tctgtgttca aggacgaagc
      361 tactgtgagg atggcagtgc aggatgcagt agatgccctg atgcagaagg ctttcaactc
      421 ctcgtccttc aactccaaca ccttcctcac caggctcctc gtgcacatgg gtctgctcaa
      481 gagtgaagac aaggtcaagg ccattgccaa cctgtacggc cccctgatgg cgctgaacca
      541 catggtgcag caggactatt tccccaaggc ccttgcaccc ctgctgctgg cgttcgtgac
      601 caagcccaac agcgccctgg aatcctgctc cttcgcccgc cacagtctgc tgcagacgct
      661 gtacaaggtc tagctcgagc atgcatctag agggccctat tctatagtgt cacctaaatg
      721 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
      781 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
      841 atgaggaaat tgcatcgcan tgtctgagta ggtgtcattc tattctgggg ggtgggggtg
      901 gggcaggaca gcaaggggga ggantgggaa gacaatagca ancatgctgg ggatgcgg
//
