LOCUS       sequence-311320-        1931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AHL-responsive single-layered circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Wan X, Pinto F, Yu L, Wang B
  TITLE     Synthetic protein-binding DNA sponge as a tool to tune gene 
            expression and mitigate protein toxicity.
  JOURNAL   Nat Commun. 2020 Nov 24;11(1):5961. doi: 10.1038/s41467-020-19552-9.
  PUBMED    33235249
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-11-24"; volume: "11(1):5961. doi"; pages: " 
            10.1038/s41467-020-19552-9"
COMMENT     Sequence Label: pXW101Plux2-gfp
FEATURES             Location/Qualifiers
     source          1..1931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..35
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     RBS             47..58
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      862..933
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      949..976
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             1057..1068
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1075..1788
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1105..1133)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1728..1748
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1811..1882
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1898..1925
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagattaaag aggagaaata
       61 ctagatgaaa aacataaatg ccgacgacac atacagaata attaataaaa ttaaagcttg
      121 tagaagcaat aatgatatta atcaatgctt atctgatatg actaaaatgg tacattgtga
      181 atattattta ctcgcgatca tttatcctca ttctatggtt aaatctgata tttcaatcct
      241 agataattac cctaaaaaat ggaggcaata ttatgatgac gctaatttaa taaaatatga
      301 tcctatagta gattattcta actccaatca ttcaccaatt aattggaata tatttgaaaa
      361 caatgctgta aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat caggtcttat
      421 cactgggttt agtttcccta ttcatacggc taacaatggc ttcggaatgc ttagttttgc
      481 acattcagaa aaagacaact atatagatag tttattttta catgcgtgta tgaacatacc
      541 attaattgtt ccttctctag ttgataatta tcgaaaaata aatatagcaa ataataaatc
      601 aaacaacgat ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag
      661 ctcttgggat atttcaaaaa tattaggttg cagtgagcgt actgtcactt tccatttaac
      721 caatgcgcaa atgaaactca atacaacaaa ccgctgccaa agtatttcta aagcaatttt
      781 aacaggagca attgattgcc catactttaa aaattaataa cactgatagt gctagtgtag
      841 atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      901 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
      961 ggtgggcctt tctgcgttta tatactagag acctgtagga tcgtacaggt ttacgcaaga
     1021 aaatggtttg ttactttcga ataaatacta gagattaaag aggagaaata ctagatgcgt
     1081 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     1141 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
     1201 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
     1261 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac
     1321 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
     1381 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
     1441 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
     1501 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
     1561 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
     1621 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc
     1681 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
     1741 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag
     1801 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1861 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1921 ctgcgtttat a
//
