LOCUS       sequence-311333-         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5 repeats of Ptet2 sponge.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Wan X, Pinto F, Yu L, Wang B
  TITLE     Synthetic protein-binding DNA sponge as a tool to tune gene 
            expression and mitigate protein toxicity.
  JOURNAL   Nat Commun. 2020 Nov 24;11(1):5961. doi: 10.1038/s41467-020-19552-9.
  PUBMED    33235249
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-11-24"; volume: "11(1):5961. doi"; pages: " 
            10.1038/s41467-020-19552-9"
COMMENT     Sequence Label: pXWS5Ptet2
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..59
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    66..84
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    186..204
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    281..299
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    306..324
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    401..419
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    426..444
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    521..539
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    546..564
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      613..684
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      700..727
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 atcgatttta gagctgcctt ttcagcagga cgcactgacc tccctatcag tgatagagat
       61 tgacatccct atcagtgata gagatactga gcacatatct taggtgtccc tagaatgagt
      121 atcgatttta gagctgcctt ttcagcagga cgcactgacc tccctatcag tgatagagat
      181 tgacatccct atcagtgata gagatactga gcacatatct taggtgtccc tagaatttcc
      241 atcgatttta gagctgcctt ttcagcagga cgcactgacc tccctatcag tgatagagat
      301 tgacatccct atcagtgata gagatactga gcacatatct taggtgtccc tagaatgact
      361 atcgatttta gagctgcctt ttcagcagga cgcactgacc tccctatcag tgatagagat
      421 tgacatccct atcagtgata gagatactga gcacatatct taggtgtccc tagaattgat
      481 atcgatttta gagctgcctt ttcagcagga cgcactgacc tccctatcag tgatagagat
      541 tgacatccct atcagtgata gagatactga gcacatatct taggtgtccc tagaattact
      601 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      661 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
      721 ttctgcgttt ata
//
