LOCUS       sequence-311335-        2545 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  20 repeats of Ptet2 sponge.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Wan X, Pinto F, Yu L, Wang B
  TITLE     Synthetic protein-binding DNA sponge as a tool to tune gene 
            expression and mitigate protein toxicity.
  JOURNAL   Nat Commun. 2020 Nov 24;11(1):5961. doi: 10.1038/s41467-020-19552-9.
  PUBMED    33235249
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-11-24"; volume: "11(1):5961. doi"; pages: " 
            10.1038/s41467-020-19552-9"
COMMENT     Sequence Label: pXWS20Ptet2
FEATURES             Location/Qualifiers
     source          1..2545
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..59
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    66..84
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    186..204
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    281..299
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    306..324
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    401..419
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    426..444
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    521..539
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    546..564
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    645..663
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    670..688
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    765..783
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    790..808
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    885..903
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    910..928
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1005..1023
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1030..1048
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1125..1143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1150..1168
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1249..1267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1274..1292
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1369..1387
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1394..1412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1489..1507
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1514..1532
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1609..1627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1634..1652
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1729..1747
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1754..1772
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1853..1871
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1878..1896
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1973..1991
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1998..2016
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2093..2111
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2118..2136
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2213..2231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2238..2256
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2333..2351
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2358..2376
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      2425..2496
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2512..2539
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 atcgatttta gagctgcctt ttcagcagga cgcactgacc tccctatcag tgatagagat
       61 tgacatccct atcagtgata gagatactga gcacatatct taggtgtccc tagaatgagt
      121 atcgatttta gagctgcctt ttcagcagga cgcactgacc tccctatcag tgatagagat
      181 tgacatccct atcagtgata gagatactga gcacatatct taggtgtccc tagaatttcc
      241 atcgatttta gagctgcctt ttcagcagga cgcactgacc tccctatcag tgatagagat
      301 tgacatccct atcagtgata gagatactga gcacatatct taggtgtccc tagaatgact
      361 atcgatttta gagctgcctt ttcagcagga cgcactgacc tccctatcag tgatagagat
      421 tgacatccct atcagtgata gagatactga gcacatatct taggtgtccc tagaattgat
      481 atcgatttta gagctgcctt ttcagcagga cgcactgacc tccctatcag tgatagagat
      541 tgacatccct atcagtgata gagatactga gcacatatct taggtgtccc tagaattact
      601 agagatcgat tttagagctg ccttttcagc aggacgcact gacctcccta tcagtgatag
      661 agattgacat ccctatcagt gatagagata ctgagcacat atcttaggtg tccctagaat
      721 gagtatcgat tttagagctg ccttttcagc aggacgcact gacctcccta tcagtgatag
      781 agattgacat ccctatcagt gatagagata ctgagcacat atcttaggtg tccctagaat
      841 ttccatcgat tttagagctg ccttttcagc aggacgcact gacctcccta tcagtgatag
      901 agattgacat ccctatcagt gatagagata ctgagcacat atcttaggtg tccctagaat
      961 gactatcgat tttagagctg ccttttcagc aggacgcact gacctcccta tcagtgatag
     1021 agattgacat ccctatcagt gatagagata ctgagcacat atcttaggtg tccctagaat
     1081 tgatatcgat tttagagctg ccttttcagc aggacgcact gacctcccta tcagtgatag
     1141 agattgacat ccctatcagt gatagagata ctgagcacat atcttaggtg tccctagaat
     1201 tactagagat cgattttaga gctgcctttt cagcaggacg cactgacctc cctatcagtg
     1261 atagagattg acatccctat cagtgataga gatactgagc acatatctta ggtgtcccta
     1321 gaatgagtat cgattttaga gctgcctttt cagcaggacg cactgacctc cctatcagtg
     1381 atagagattg acatccctat cagtgataga gatactgagc acatatctta ggtgtcccta
     1441 gaatttccat cgattttaga gctgcctttt cagcaggacg cactgacctc cctatcagtg
     1501 atagagattg acatccctat cagtgataga gatactgagc acatatctta ggtgtcccta
     1561 gaatgactat cgattttaga gctgcctttt cagcaggacg cactgacctc cctatcagtg
     1621 atagagattg acatccctat cagtgataga gatactgagc acatatctta ggtgtcccta
     1681 gaattgatat cgattttaga gctgcctttt cagcaggacg cactgacctc cctatcagtg
     1741 atagagattg acatccctat cagtgataga gatactgagc acatatctta ggtgtcccta
     1801 gaattactag agatcgattt tagagctgcc ttttcagcag gacgcactga cctccctatc
     1861 agtgatagag attgacatcc ctatcagtga tagagatact gagcacatat cttaggtgtc
     1921 cctagaatga gtatcgattt tagagctgcc ttttcagcag gacgcactga cctccctatc
     1981 agtgatagag attgacatcc ctatcagtga tagagatact gagcacatat cttaggtgtc
     2041 cctagaattt ccatcgattt tagagctgcc ttttcagcag gacgcactga cctccctatc
     2101 agtgatagag attgacatcc ctatcagtga tagagatact gagcacatat cttaggtgtc
     2161 cctagaatga ctatcgattt tagagctgcc ttttcagcag gacgcactga cctccctatc
     2221 agtgatagag attgacatcc ctatcagtga tagagatact gagcacatat cttaggtgtc
     2281 cctagaattg atatcgattt tagagctgcc ttttcagcag gacgcactga cctccctatc
     2341 agtgatagag attgacatcc ctatcagtga tagagatact gagcacatat cttaggtgtc
     2401 cctagaatta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     2461 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     2521 tcgggtgggc ctttctgcgt ttata
//
