LOCUS       sequence-311343-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  10 repeats of Pecf11 sponge.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Wan X, Pinto F, Yu L, Wang B
  TITLE     Synthetic protein-binding DNA sponge as a tool to tune gene 
            expression and mitigate protein toxicity.
  JOURNAL   Nat Commun. 2020 Nov 24;11(1):5961. doi: 10.1038/s41467-020-19552-9.
  PUBMED    33235249
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-11-24"; volume: "11(1):5961. doi"; pages: " 
            10.1038/s41467-020-19552-9"
COMMENT     Sequence Label: pXWS10Pecf11
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      903..974
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      990..1017
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 atcgatttta gagctgccgc ctccacaccg ctcgtcacat cctgtgatcc actcttcatc
       61 ccgctacgta acacctctgt actcagctta tctcgggcct ccacaccgct cgtcacatcc
      121 tgtgatccac tcttcatccc gctacgtaac acctctctta ggtgtcccta gaatgagtat
      181 cgattttaga gctgccgcct ccacaccgct cgtcacatcc tgtgatccac tcttcatccc
      241 gctacgtaac acctctgtac tcagcttatc tcgggcctcc acaccgctcg tcacatcctg
      301 tgatccactc ttcatcccgc tacgtaacac ctctcttagg tgtccctaga atttccatcg
      361 attttagagc tgccgcctcc acaccgctcg tcacatcctg tgatccactc ttcatcccgc
      421 tacgtaacac ctctgtactc agcttatctc gggcctccac accgctcgtc acatcctgtg
      481 atccactctt catcccgcta cgtaacacct ctcttaggtg tccctagaat gactatcgat
      541 tttagagctg ccgcctccac accgctcgtc acatcctgtg atccactctt catcccgcta
      601 cgtaacacct ctgtactcag cttatctcgg gcctccacac cgctcgtcac atcctgtgat
      661 ccactcttca tcccgctacg taacacctct cttaggtgtc cctagaattg atatcgattt
      721 tagagctgcc gcctccacac cgctcgtcac atcctgtgat ccactcttca tcccgctacg
      781 taacacctct gtactcagct tatctcgggc ctccacaccg ctcgtcacat cctgtgatcc
      841 actcttcatc ccgctacgta acacctctct taggtgtccc tagaattact agagccaggc
      901 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      961 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1021 ata
//
